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    Method for isolating and purifying nucleic acids
    3.
    发明授权
    Method for isolating and purifying nucleic acids 有权
    分离和纯化核酸的方法

    公开(公告)号：US09163228B2

    公开(公告)日：2015-10-20

    申请号：US13639808

    申请日：2011-04-08

    申请人： Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

    发明人： Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1003 ,  C12N15/101

    摘要： The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

    摘要翻译： 本发明涉及用于分离和纯化来自生物样品的核酸的优选包含基因组DNA的方法，包括以下步骤：使用包含阴离子表面活性剂离子源的裂解缓冲液裂解样品，任选地分解裂解物中存在的RNA， 从裂解物沉淀表面活性剂离子，并通过尺寸排阻色谱法将沉淀物和其它污染物分离出来。 本发明还涉及裂解缓冲液，裂解细胞的方法和用于分离和纯化核酸的试剂盒。

    METHOD FOR ISOLATING AND PURIFYING NUCLEIC ACIDS
    4.
    发明申请
    METHOD FOR ISOLATING AND PURIFYING NUCLEIC ACIDS 有权
    分离和纯化核酸的方法

    公开(公告)号：US20130030163A1

    公开(公告)日：2013-01-31

    申请号：US13639808

    申请日：2011-04-08

    申请人： Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

    发明人： Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

    IPC分类号： C07H21/04 ,  B01D15/08 ,  C09K3/00 ,  C12N9/50 ,  C09K15/02

    CPC分类号： C12N15/1003 ,  C12N15/101

    摘要： The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

    摘要翻译： 本发明涉及用于分离和纯化来自生物样品的核酸的优选包含基因组DNA的方法，包括以下步骤：使用包含阴离子表面活性剂离子源的裂解缓冲液裂解样品，任选地分解裂解物中存在的RNA， 从裂解物沉淀表面活性剂离子，并通过尺寸排阻色谱法将沉淀物和其它污染物分离出来。 本发明还涉及裂解缓冲液，裂解细胞的方法和用于分离和纯化核酸的试剂盒。

    WATER-SOLUBLE POLYMERS HAVING CHELATORS
    7.
    发明申请
    WATER-SOLUBLE POLYMERS HAVING CHELATORS 审中-公开
    具有洗衣机的水溶性聚合物

    公开(公告)号：US20100261289A1

    公开(公告)日：2010-10-14

    申请号：US12680932

    申请日：2008-10-10

    申请人： Roland Fabis ,  Bernd Springer

    发明人： Roland Fabis ,  Bernd Springer

    IPC分类号： G01N21/00 ,  C08F12/08 ,  C08F10/06 ,  C08F20/18 ,  C08F20/56

    CPC分类号： C08F8/30

    摘要： The present invention relates to a functionalized polymer having a high solubility in at least one solvent having high Eτ(30) value, and being functionalized with at least one N-containing carboxylate chelator.

    摘要翻译： 本发明涉及在具有高Eτ（30）值的至少一种溶剂中具有高溶解度并且用至少一种含N羧酸盐螯合剂官能化的官能化聚合物。

    NUCLEIC ACID PURIFICATION METHOD
    10.
    发明申请
    NUCLEIC ACID PURIFICATION METHOD 有权
    核酸纯化方法

    公开(公告)号：US20110319506A1

    公开(公告)日：2011-12-29

    申请号：US13141878

    申请日：2009-12-23

    申请人： Christoph Erbacher ,  Roland Fabis

    发明人： Christoph Erbacher ,  Roland Fabis

    IPC分类号： B01J41/14 ,  C07F7/18 ,  C07H21/00

    CPC分类号： C12N15/1006

    摘要： The present invention relates to a method for purifying nucleic acids using a nucleic acid-binding phase which is furnished with a deficit of nucleic acid-binding groups A having a pK of 8 to 13, or which has groups A and binding-inhibiting groups N which are neutrally charged during the binding, and preferably during the elution, and the method comprises the following steps: (a) binding the nucleic acids to the nucleic acid-binding phase at a pH which is below the pH of the pK of the nucleic acid-binding groups A (binding pH); (b) eluting the nucleic acids at a pH which is above the binding pH (elution pH). In addition, corresponding kits and also nucleic acid-binding phases which can be used for purifying nucleic acids are disclosed. The technology according to the invention permits the purification of nucleic acids and, in particular, elution, with use of low salt concentrations, and so the purified nucleic acids can be directly processed, for example used in a PCR.

    摘要翻译： 本发明涉及使用核酸结合相提纯核酸的方法，所述核酸结合相具有pK为8〜13的核酸结合基团A的缺陷，或具有A基团和结合抑制基团N 其在结合期间是中性带电的，优选在洗脱过程中，并且该方法包括以下步骤：（a）在低于核酸pK的pH的pH下将核酸与核酸结合相结合 酸结合基团A（结合pH）; （b）在高于结合pH（洗脱pH）的pH下洗脱核酸。 此外，公开了可用于纯化核酸的相应试剂盒和核酸结合相。 根据本发明的技术允许核酸的纯化，特别是使用低盐浓度洗脱，因此纯化的核酸可以直接加工，例如用于PCR中。