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公开(公告)号：US20110086427A1
公开(公告)日：2011-04-14
申请号：US12716680
申请日：2010-03-03
摘要： A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.
摘要翻译： 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。
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公开(公告)号：US08481303B2
公开(公告)日：2013-07-09
申请号：US12716680
申请日：2010-03-03
IPC分类号： C12M3/00
摘要： A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.
摘要翻译： 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。
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公开(公告)号：US07566567B2
公开(公告)日：2009-07-28
申请号：US10574163
申请日：2004-10-07
申请人： Jin Liu , Ronald A. Faris
发明人： Jin Liu , Ronald A. Faris
CPC分类号： C12N5/067 , A61K38/00 , C07K14/47 , C12N2503/02 , C12N2510/02 , C12N2510/04 , G01N33/5067 , G01N2333/90245 , Y02A50/467
摘要： This invention relates to virally-immortalized hepatocyte cell lines, which are derived from a normal primary human liver cell, have the ability to proliferate in a serum-free media, are nontumorigenic, and produce proteins. These cell lines can be used for toxicity testing of potential therapeutic drugs and chemical entities. The cell lines may also be used for the production of therapeutic plasma proteins.
摘要翻译： 本发明涉及源自正常原代人肝细胞的病毒永生化肝细胞细胞系，具有在无血清培养基中增殖的能力，是非致癌性的，并且产生蛋白质。 这些细胞系可用于潜在的治疗药物和化学实体的毒性测试。 细胞系也可用于生产治疗性血浆蛋白。
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公开(公告)号：US06872389B1
公开(公告)日：2005-03-29
申请号：US09743544
申请日：1999-07-08
申请人： Ronald A. Faris
发明人： Ronald A. Faris
CPC分类号： C12N5/0672 , C12N2501/58
摘要： The invention provides methods of isolating a primary liver stem cell by identifying a cell doublet containing a hepatocytes and the stem cell and isolating the doublet from liver tissue.
摘要翻译： 本发明提供了通过鉴定含有肝细胞和干细胞并从肝组织分离双链体的细胞双体分离原代肝干细胞的方法。
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公开(公告)号：US20100304427A1
公开(公告)日：2010-12-02
申请号：US12788033
申请日：2010-05-26
CPC分类号： C12N5/0068 , C12N2533/30
摘要： The present invention provides substrates useful for culturing cells under low serum or serum free conditions. The substrates are useful for conducting cell-based assays where there is interference from serum proteins. Methods are also provided for using the substrates of the present invention as well as methods for making the substrates of the present invention.
摘要翻译： 本发明提供了用于在低血清或无血清条件下培养细胞的底物。 底物可用于进行基于细胞的测定，其中存在来自血清蛋白质的干扰。 还提供了使用本发明的基材的方法以及制备本发明的基材的方法。
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公开(公告)号：US6129911A
公开(公告)日：2000-10-10
申请号：US113774
申请日：1998-07-10
申请人： Ronald A. Faris
发明人： Ronald A. Faris
CPC分类号： C12N5/0672 , A61K35/12 , C12N2510/00
摘要： The invention provides a primary liver stem cell and a cell doublet consisting of a hepatocyte and the stem cell, both of which are derived from normal liver tissue. Methods of isolating the cells, genetically altering the cells, and using the cells for transplantation are also within the invention.
摘要翻译： 本发明提供了一种原代肝干细胞和由肝细胞和干细胞组成的细胞双重体，两者均源于正常的肝组织。 分离细胞，遗传改变细胞和使用细胞进行移植的方法也在本发明内。
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公开(公告)号：US07935528B2
公开(公告)日：2011-05-03
申请号：US11039119
申请日：2005-01-19
申请人： Ronald A. Faris
发明人： Ronald A. Faris
CPC分类号： C12N5/0672 , C12N2501/58
摘要： The invention provides a primary liver stem cell and a cell doublet consisting of a hepatocyte and the stem cell, both of which are derived from normal liver tissue. Methods of isolating the cells, genetically altering the cells, and using the cells for transplantation are also within the invention.
摘要翻译： 本发明提供了一种原代肝干细胞和由肝细胞和干细胞组成的细胞双重体，两者均源于正常的肝组织。 分离细胞，遗传改变细胞和使用细胞进行移植的方法也在本发明内。
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