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    Method to derivatize dextran
    2.
    发明授权
    Method to derivatize dextran 失效
    衍生葡聚糖的方法

    公开(公告)号：US4975532A

    公开(公告)日：1990-12-04

    申请号：US137986

    申请日：1987-12-24

    申请人： Gerald L. Rowley ,  Larry R. Hillis

    发明人： Gerald L. Rowley ,  Larry R. Hillis

    IPC分类号： C08B37/02 ,  G01N33/532

    CPC分类号： G01N33/532 ,  C08B37/0021

    摘要： A method to convert the hydroxyl groups of solubilized dextran to the tresylate forms which can be displaced by a nucleophile is disclosed. The method of the invention requires the reaction of dextran with the tresylating reagent in the presence of dry DMSO. The resulting tresylate can be converted to labeled dextrans which have direct linkages of a nucleophilic label to the carbons of the dextran backbone.

    摘要翻译： 公开了将可溶性葡聚糖的羟基转化为可被亲核试剂置换的三氟甲磺酸酯形式的方法。 本发明的方法需要在干燥的DMSO存在下使葡聚糖与三聚反应试剂反应。 所得到的三氟甲磺酸酯可转化为具有亲核标记与葡聚糖主链碳原子的直接连接的标记的葡聚糖。

    Double antibody for enhanced sensitivity in immunoassay
    3.
    发明授权
    Double antibody for enhanced sensitivity in immunoassay 失效
    双抗体，增强免疫测定的灵敏度

    公开(公告)号：US4281061A

    公开(公告)日：1981-07-28

    申请号：US61542

    申请日：1979-07-27

    申请人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    发明人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    IPC分类号： C12N9/96 ,  G01N33/542 ,  G01N31/14 ,  G01N33/48 ,  G01N33/54

    CPC分类号： G01N33/542 ,  C12N9/96 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/971 ,  Y10S436/80 ,  Y10S436/808

    摘要： Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides a detectable signal, which system includes a macromolecular member. The determination of the analyte, which is a member of a specific binding pair consisting of a ligand and its homologous receptor, is performed by creating an extensive matrix in the assay medium by having in the assay medium in addition to the analyte, ligand labeled with one of the members of the signal producing system, antiligand either present as the analyte or added, a polyvalent receptor for antiligand, the macromolecular member of the signal producing system, and any additional members of the signal producing system. The labeled ligand, antiligand, and polyvalent receptor for the antiligand create a matrix which modulates, e.g. inhibits, the approach of the macromolecular member of the signal producing system to the labeled ligand. The extent and degree of formation of the matrix is dependent upon the concentration of the analyte in the medium. By comparing the signal from an assay medium having an unknown amount of analyte, with a signal obtained from an assay medium having a known amount of analyte, the amount of analyte in the unknown sample may be determined qualitatively or quantitatively.Kits are provided having predetermined amounts of the various reagents to allow for enhanced sensitivity of the method.

    摘要翻译： 提供方法和组合物用于进行均匀的免疫测定。 该方法包括具有提供可检测信号的信号产生系统，该系统包括大分子成员。 由配体及其同源受体组成的特异性结合对成员的分析物的测定通过在测定培养基中通过在测定培养基中除了分析物之外产生广泛的基质来进行，所述分析物被标记有 信号生成系统的成员之一，反配位体作为分析物存在或添加，反配体的多价受体，信号产生系统的大分子成员以及信号产生系统的任何附加成员。 用于反配体的标记的配体，反配体和多价受体产生调节的基质。 抑制信号产生系统的大分子成员对标记的配体的接近。 基质的形成程度取决于培养基中分析物的浓度。 通过比较来自具有未知量分析物的测定培养基的信号与从具有已知量分析物的测定培养基获得的信号，可以定性或定量地确定未知样品中分析物的量。 提供具有预定量的各种试剂以提供该方法的增强灵敏度的试剂盒。

    Concentrating zone method in heterogeneous immunoassays
    4.
    发明授权
    Concentrating zone method in heterogeneous immunoassays 失效
    异种免疫测定中的浓缩区法

    公开(公告)号：US4366241A

    公开(公告)日：1982-12-28

    申请号：US176177

    申请日：1980-08-07

    申请人： Henry K. Tom ,  Gerald L. Rowley

    发明人： Henry K. Tom ,  Gerald L. Rowley

    IPC分类号： G01N33/543 ,  G01N33/558 ,  G01N33/54 ,  C12Q1/70 ,  G01N33/16

    CPC分类号： G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/807

    摘要： Method and apparatus are provided for performing immunoassays employing a device comprising a relatively small test zone referred to as an immunosorbing zone, and a relatively large liquid absorbing zone in liquid receiving relationship with said immunosorbing zone. The immunosorbing zone includes a member of an immunological pair ("mip")--ligand and antiligand--bound to a support.A signal producing system is employed in conjunction with said device having as one component a signal label bound to a mip. The signal producing system provides for production of a detectible signal in the immunosorbing zone in relation to the amount of analyte in a sample.

    摘要翻译： 提供了用于进行使用包括称为免疫吸附区的相对小的测试区的装置的免疫测定方法和装置，以及与所述免疫吸附区接受关系的液体相对较大的液体吸收区。 免疫吸附区包括免疫对（“mip”） - 配体的成员和与支持物结合的配配体。 信号产生系统与所述设备结合使用，该设备具有绑定到mip的信号标签。 信号产生系统提供了在免疫吸附区中相对于样品中分析物的量的可检测信号的产生。

    Charge effects in enzyme immunoassays
    5.
    发明授权
    Charge effects in enzyme immunoassays 失效
    酶免疫测定中的电荷效应

    公开(公告)号：US4287300A

    公开(公告)日：1981-09-01

    申请号：US61099

    申请日：1979-07-26

    申请人： Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    发明人： Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

    IPC分类号： G01N33/536 ,  C12Q1/66 ,  G01N21/00 ,  G01N33/531 ,  G01N33/542 ,  C12Q1/70 ,  C12N9/96 ,  G01N33/54

    CPC分类号： G01N33/542 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/82

    摘要： A method for determining a member of a specific binding pair-ligand and receptor (antiligand). Reagents employed include a first modified member which provides an electrical field due to the presence of a plurality of ionic charges and a second modified member labeled with a component of a signal producing system, which system may have one or more components. The average proximity in the assay medium of the first and second modified members is related to the amount of analyte, where the observed signal from the signal producing system is related to the effect of the electrical field on the signal producing system.Also, compositions are provided, as well as reagents, in predetermined ratios for optimizing the signal response to variations in analyte concentration.

    摘要翻译： 用于测定特异性结合对 - 配体和受体（反配体）的成员的方法。 使用的试剂包括由于存在多个离子电荷而提供电场的第一改性构件和用信号产生系统的组分标记的第二修饰构件，该系统可以具有一个或多个组分。 第一和第二修饰构件的测定介质中的平均接近度与分析物的量相关，其中来自信号产生系统的观测信号与电场对信号产生系统的影响有关。 此外，提供了以预定比例的组合物以及试剂，以优化对分析物浓度变化的信号响应。

    Enzyme-aminoglycoside conjugates
    7.
    发明授权
    Enzyme-aminoglycoside conjugates 失效
    酶 - 氨基糖苷共轭物

    公开(公告)号：US4328311A

    公开(公告)日：1982-05-04

    申请号：US125713

    申请日：1980-02-28

    申请人： Gerald L. Rowley ,  Danton Leung ,  Prithipal Singh

    发明人： Gerald L. Rowley ,  Danton Leung ,  Prithipal Singh

    IPC分类号： C12N9/96 ,  G01N33/535

    CPC分类号： C12N9/96 ,  G01N33/535 ,  Y10S530/806

    摘要： Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkyl-carbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.

    摘要翻译： 提供了将多种具有巯基的化合物，特别是多官能化合物，天然存在或合成引入的多种化合物，特别是多肽（包括蛋白质）共轭的方法和组合物。 该方法采用卤代烷基 - 羰基化合物，其在轻度酰化条件下与一个或多个氨基结合。 然后将酰化的聚氨基化合物与含巯基的化合物结合，由此卤素被巯基的硫取代，形成稳定的硫醚键。 取决于所涉及的化合物，得到的缀合物可用于免疫测定中，作为用于产生抗体的半抗原共轭体，以及用作控制巯基化合物取代聚氨基酸的配体的配体类似物酶缀合物 复合。

    Method for conjugating to polyamino compounds employing haloacyl groups and compositions prepared thereby
    8.
    发明授权
    Method for conjugating to polyamino compounds employing haloacyl groups and compositions prepared thereby 失效
    使用卤代酰基与多氨基化合物共轭的方法和由此制备的组合物

    公开(公告)号：US4220722A

    公开(公告)日：1980-09-02

    申请号：US876772

    申请日：1978-02-10

    申请人： Gerald L. Rowley ,  Danton Leung ,  Prithiphal Singh

    发明人： Gerald L. Rowley ,  Danton Leung ,  Prithiphal Singh

    IPC分类号： G01N33/535 ,  C12N9/96

    CPC分类号： G01N33/535 ,  Y10S435/961 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/823 ,  Y10S530/806

    摘要： Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkylcarbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.

    摘要翻译： 提供了将多种具有巯基的化合物，特别是多官能化合物，天然存在或合成引入的多种化合物，特别是多肽（包括蛋白质）共轭的方法和组合物。 该方法采用卤代烷基羰基化合物，其在温和的酰化条件下与一个或多个氨基相连。 然后将酰化的聚氨基化合物与含巯基的化合物结合，由此卤素被巯基的硫取代，形成稳定的硫醚键。 取决于所涉及的化合物，得到的缀合物可用于免疫测定中，作为产生抗体的半抗原共轭体，以及用作控制巯基化合物取代聚氨基酸的配体的配体类似物酶偶联物 复合。