利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

27 条记录 (耗时 0.037 秒)

    Well purge and sample apparatus and method
    1.
    发明授权
    Well purge and sample apparatus and method 失效
    良好的吹扫和取样装置和方法

    公开(公告)号：US5460224A

    公开(公告)日：1995-10-24

    申请号：US112829

    申请日：1993-08-26

    申请人： Ronald Schalla ,  Ronald M. Smith ,  Stephen H. Hall ,  John E. Smart ,  Gregg S. Gustafson

    发明人： Ronald Schalla ,  Ronald M. Smith ,  Stephen H. Hall ,  John E. Smart ,  Gregg S. Gustafson

    IPC分类号： E21B43/12 ,  E21B49/08

    CPC分类号： E21B49/084 ,  E21B43/121 ,  E21B49/082

    摘要： The present invention specifically permits purging and/or sampling of a well but only removing, at most, about 25% of the fluid volume compared to conventional methods and, at a minimum, removing none of the fluid volume from the well.The invention is an isolation assembly with a packer, pump and exhaust, that is inserted into the well. The isolation assembly is designed so that only a volume of fluid between the outside diameter of the isolation assembly and the inside diameter of the well over a fluid column height from the bottom of the well to the top of the active portion (lower annulus) is removed. The packer is positioned above the active portion thereby sealing the well and preventing any mixing or contamination of inlet fluid with fluid above the packer. Ports in the wall of the isolation assembly permit purging and sampling of the lower annulus along the height of the active portion.

    摘要翻译： 本发明具体允许清洗和/或取样一个井，但与常规方法相比只能去除至多约25％的流体体积，并且至少从井中不去除流体体积。 本发明是一种具有封隔器，泵和排气的隔离组件，其被插入井中。 隔离组件被设计成使得在隔离组件的外径和从井的底部到活动部分（下环空间）的顶部之间的流体柱高度的井的内径之间仅有一定体积的流体是 删除。 封隔器位于有效部分上方，从而密封井，并防止入口流体与封隔器上方的流体混合或污染。 隔离组件的壁中的端口允许沿着有效部分的高度清洗和取样下环。

    Well fluid isolation and sample apparatus and method
    2.
    发明授权
    Well fluid isolation and sample apparatus and method 失效
    良好的液体隔离和取样装置及方法

    公开(公告)号：US5450900A

    公开(公告)日：1995-09-19

    申请号：US271609

    申请日：1994-07-07

    申请人： Ronald Schalla ,  Ronald M. Smith ,  Stephen H. Hall ,  John E. Smart

    发明人： Ronald Schalla ,  Ronald M. Smith ,  Stephen H. Hall ,  John E. Smart

    IPC分类号： E21B43/12 ,  E21B49/08 ,  E21B43/00

    CPC分类号： E21B49/084 ,  E21B43/121

    摘要： The present invention specifically permits purging and/or sampling of a well but only removing, at most, about 25% of the fluid volume compared to conventional methods and, at a minimum, removing none of the fluid volume from the well.The invention is an isolation assembly that is inserted into the well. The isolation assembly is designed so that only a volume of fluid between the outside diameter of the isolation assembly and the inside diameter of the well over a fluid column height from the bottom of the well to the top of the active portion (lower annulus) is removed. A seal may be positioned above the active portion thereby sealing the well and preventing any mixing or contamination of inlet fluid with fluid above the packer. Purged well fluid is stored in a riser above the packer. Ports in the wall of the isolation assembly permit purging and sampling of the lower annulus along the height of the active portion.

    摘要翻译： 本发明具体允许清洗和/或取样一个井，但与常规方法相比只能去除至多约25％的流体体积，并且至少从井中不去除流体体积。 本发明是插入井中的隔离组件。 隔离组件被设计成使得在隔离组件的外径和从井的底部到活动部分（下环空间）的顶部之间的流体柱高度的井的内径之间仅有一定体积的流体是 删除。 密封件可以位于活性部分上方，从而密封井，并防止进口流体与封隔器上方的流体混合或污染。 净化的流体储存在封隔器上方的提升管中。 隔离组件的壁中的端口允许沿着有效部分的高度清洗和取样下环。

    Purification of chimeric proteins containing an IL-2 moiety by receptor-affinity chromatography
    9.
    发明授权
    Purification of chimeric proteins containing an IL-2 moiety by receptor-affinity chromatography 失效
    通过受体亲和层析纯化含有IL-2部分的嵌合蛋白

    公开(公告)号：US5149788A

    公开(公告)日：1992-09-22

    申请号：US261198

    申请日：1988-10-24

    申请人： Pascal Bailon ,  John E. Smart ,  David V. Weber

    发明人： Pascal Bailon ,  John E. Smart ,  David V. Weber

    IPC分类号： C07K14/55

    CPC分类号： C07K14/55 ,  Y10S530/81 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/813 ,  Y10S530/814

    摘要： Methods are provided for the purification of interleukin-2 (IL-2) and chimeric proteins containing an IL-2 moiety from a wide variety of sources, including synthetic mixtures, cell culture conditioned medium and mammalian and bacterial recombinant expression systems. The methods of the invention employ IL-2 receptor-affinity adsorbents in which soluble IL-2 receptors have been immobilized on solid supports. Through the use of these affinity adsorbents, highly purified IL-2 and chimeric proteins containing an IL-2 moiety can be produced in a single step from bacterial extracts or conditioned medium. The proteins thus purified are largely free of aggregated forms, which are often present when other purification methods are used.

    摘要翻译： 提供了用于从多种来源（包括合成混合物，细胞培养条件培养基和哺乳动物和细菌重组表达系统）纯化白细胞介素-2（IL-2）和含有IL-2部分的嵌合蛋白的方法。 本发明的方法使用其中可溶性IL-2受体已经固定在固体支持物上的IL-2受体亲和吸附剂。 通过使用这些亲和吸附剂，可以从细菌提取物或条件培养基中单步制备高纯度的IL-2和含有IL-2部分的嵌合蛋白质。 如此纯化的蛋白质基本上不含聚集形式，当使用其它纯化方法时，它们通常存在。