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1.发明授权Method for increasing the enzymatic reactivity of .beta.-galactosidase by addition of a cyanate, thiocyanate, azide, or thiosulfate compound 失效通过加入氰酸酯，硫氰酸酯，亚胺或硫代脂肪酸酯化合物来增加（BETA） - 乳糖苷酶的酶促反应活性的方法
公开(公告)号：US5194382A
公开(公告)日：1993-03-16
申请号：US609242
申请日：1990-11-05
CPC分类号： C12Q1/54
摘要： In order to increase the enzymatic reactivity of .beta.-galactosidase, an azide, thiocyanate, cyanate and/or thiosulphate is added to the reaction mixture.
摘要翻译： 为了增加β-半乳糖苷酶的酶反应性，向反应混合物中加入叠氮化物，硫氰酸酯，氰酸酯和/或硫代硫酸盐。
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公开(公告)号：US5204242A
公开(公告)日：1993-04-20
申请号：US761335
申请日：1991-09-17
IPC分类号： C07C37/00 , C07C39/24 , C07C39/27 , C07C43/178 , C07C59/52 , C07C59/56 , C07C69/732 , C07C205/56 , C07C215/48 , C07C215/66 , C07C235/34 , C07C309/24 , C07C309/68 , C07C311/13 , C07C311/27 , G01N31/22 , G03C7/34
CPC分类号： C07C235/34 , C07C205/56 , C07C309/24 , C07C311/27 , C07C39/27 , C07C59/52 , C07C59/56 , G03C7/346
摘要： The present invention provides a colorimetric process for the detection of an oxidative coupling reaction in which a coupling component is reacted with a developer component in the presence of an oxidation agent to give a colored material, wherein, as coupling component, there is used a compound of the general formula: ##STR1## in which X is a bromine or chlorine atom, n is 1, 2 or 3, Y is COOR, CONRR', SO.sub.3 R, SO.sub.2 NRR', OR, NRR' or NRR'R".sym. and R, R' and R", independently of one another, are hydrogen atoms or C.sub.1 -C.sub.3 -alkyl radicals.The present invention also provides a reagent which contains the above compound, as well as new substituted phenols and a process for the preparation thereof.
摘要翻译： 本发明提供了一种用于检测氧化偶联反应的比色方法，其中偶联组分在氧化剂存在下与显影剂组分反应，得到着色材料，其中作为偶联组分，使用化合物 其中X是溴或氯原子，其中n是1,2或3，Y是COOR，CONRR'，SO3R，SO2NRR'，OR，NRR'或NRR'R “（+）”和“R”，“R”和“R”彼此独立地是氢原子或C1-C3-烷基。 本发明还提供了含有上述化合物的试剂，以及新的取代酚类及其制备方法。
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公开(公告)号：US6057120A
公开(公告)日：2000-05-02
申请号：US934784
申请日：1997-09-22
申请人： Dieter Heindl , Rupert Herrmann , Joachim Hones , Hans-Peter Josel , Martina Junius-Comer , Hartmut Merdes , Axel Schmidt , Ernst Selbertinger
发明人： Dieter Heindl , Rupert Herrmann , Joachim Hones , Hans-Peter Josel , Martina Junius-Comer , Hartmut Merdes , Axel Schmidt , Ernst Selbertinger
IPC分类号： C07D241/38 , C07D241/46 , C07D401/14 , C07D471/04 , C07D487/04 , C07D491/048 , C07D495/04 , C12Q1/26 , G01N31/22 , G01N33/52 , C12Q1/00 , G01N33/53
CPC分类号： C07D241/38 , C07D401/14 , C12Q1/26 , G01N31/22 , G01N33/52 , Y10S435/968
摘要： The invention concerns the use of redox-active compounds for the production of detection reagents for a method for the determination of an analyte as well as reagent kits that contain these redox-active compounds. In addition new redox-active compounds are disclosed.
摘要翻译： 本发明涉及氧化还原活性化合物用于生产用于测定分析物的方法的检测试剂以及含有这些氧化还原活性化合物的试剂盒的用途。 此外，还公开了新的氧化还原活性化合物。
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公开(公告)号：US5260428A
公开(公告)日：1993-11-09
申请号：US988975
申请日：1993-01-29
申请人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
发明人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
CPC分类号： C12Q1/34 , G01N33/581 , C12Q2334/30 , G01N2333/924
摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidising agent, and the evaluating the resultant colour intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5, R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue. Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.
摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5，R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。
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公开(公告)号：US5292669A
公开(公告)日：1994-03-08
申请号：US927870
申请日：1992-08-10
申请人： Hans-Joachim Guder , Werner Guthlein , Wolfgang Weckerle , Johann Berger , Harvey Buck , Rupert Herrmann
发明人： Hans-Joachim Guder , Werner Guthlein , Wolfgang Weckerle , Johann Berger , Harvey Buck , Rupert Herrmann
IPC分类号： C12Q1/00 , C07D233/54 , C07D233/64 , C07D263/32 , C07D277/20 , C07D277/24 , C07D277/28 , C07D277/34 , C07F9/6506 , C07F9/653 , C07F9/6539 , C07H15/26 , C07H17/00 , C12Q1/34 , C12Q1/42 , C12Q1/44 , C07D277/82 , C07D233/56
CPC分类号： C07D233/64 , C07D263/32 , C07D277/24 , C07D277/28 , C07F9/65062 , C07F9/653 , C07F9/6539 , C07H15/26 , C12Q1/34 , C12Q1/42 , C12Q2334/00 , G01N2333/924
摘要： A method of detecting hydrolase activity using as substrates the compounds of the formula ##STR1## wherein each of R.sup.1, R.sup.2 and R.sup.3 is C.sub.1 -C.sub.4 alkyl, or phenyl optionally substituted in the meta- or para- position by C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy or mono- or di-(C.sub.1 -C.sub.6)-alkylamino, or optionally substituted by an O-X group in which X is a glycosyl, phosphate or acyl moiety of a natural substrate of the corresponding glycosidase, phosphatase or esterase.
摘要翻译： 使用作为底物检测水解酶活性的方法，其中R 1，R 2和R 3各自为C 1 -C 4烷基或任选在C 1 -C 6烷基的间位或对位取代的苯基， -C 1-6烷氧基或单 - 或二 - （C 1 -C 6） - 烷基氨基，或任选地被OX基团取代，其中X是相应糖苷酶，磷酸酶或酯酶的天然底物的糖基，磷酸酯或酰基部分。
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公开(公告)号：US5202233A
公开(公告)日：1993-04-13
申请号：US816795
申请日：1992-01-03
申请人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
发明人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
CPC分类号： C12Q1/34 , G01N33/581 , C12Q2334/30 , G01N2333/924 , Y10S435/805
摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue.Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.
摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5和R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。
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公开(公告)号：US07807401B2
公开(公告)日：2010-10-05
申请号：US12330725
申请日：2008-12-09
IPC分类号： C12Q1/37
CPC分类号： G01N33/723 , G01N2333/95
摘要： The present invention relates to a reagent for digestion of hemoglobin comprising a buffer, pepsin and a 1,3-dialkyl-imidazolium salt. It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin.
摘要翻译： 本发明涉及一种用于消化血红蛋白的试剂，其包含缓冲液，胃蛋白酶和1,3-二烷基 - 咪唑鎓盐。 还公开了在用于消化血红蛋白的方法中使用该试剂，在检测HbA1c的方法中，以及用于收集包含用于消化血红蛋白的所述试剂的全血样品的采样管。
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8.发明授权Fluorimetric determination of analytes by an intramolecular quencher-fluorophore conjugate 有权通过分子内猝灭剂 - 荧光团缀合物进行荧光测定分析物公开(公告)号：US07378255B2
公开(公告)日：2008-05-27
申请号：US10766457
申请日：2004-01-28
申请人： Carina Horn , Hans-Peter Josel , Jürgen Spinke , Rupert Herrmann , Dieter Heindl
发明人： Carina Horn , Hans-Peter Josel , Jürgen Spinke , Rupert Herrmann , Dieter Heindl
IPC分类号： C12Q1/26
CPC分类号： G01N21/6428 , G01N2021/6432 , Y10S436/80 , Y10S436/904
摘要： A method and reagent for detecting an analyte by a redox reaction and a fluorimetric determination, is disclosed. The method comprises contacting a sample containing the analyte with a detection reagent which contains a compound of the general formula Q-F as a fluorimetric redox indicator, wherein Q is a quencher group and F is a fluorophore group.
摘要翻译： 公开了一种用于通过氧化还原反应和荧光测定来检测分析物的方法和试剂。 该方法包括将含有分析物的样品与含有通式Q-F化合物的检测试剂接触，作为荧光氧化还原指示剂，其中Q为猝灭剂基团，F为荧光团。
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公开(公告)号：US20060199223A1
公开(公告)日：2006-09-07
申请号：US11326852
申请日：2006-01-06
IPC分类号： G01N33/53 , C12Q1/66 , C07D277/84 , C07D263/60 , C07K16/46
CPC分类号： C07D209/60 , G01N33/533
摘要： The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays.
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10.发明授权Method for the simultaneous determination of biomolecular interactions by means of plasmon resonance and fluoresence detection 失效通过等离子体共振和荧光检测同时测定生物分子相互作用的方法公开(公告)号：US06194223B1
公开(公告)日：2001-02-27
申请号：US09058868
申请日：1998-04-13
IPC分类号： G01N33543
CPC分类号： G01N33/582 , B82Y5/00 , B82Y15/00 , B82Y30/00 , G01N21/553 , G01N21/648 , G01N33/543 , G01N33/551 , Y10S435/808 , Y10S436/805
摘要： A method for the detection of an analyte is described which is characterized in that the binding of the analyte to a solid phase is determined by the independent analysis of the signals from a plasmon resonance measurement and a fluorescence measurement.
摘要翻译： 描述了用于检测分析物的方法，其特征在于通过对来自等离子体共振测量和荧光测量的信号的独立分析来确定分析物与固相的结合。
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