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    Fluorescent polypeptide complex
    6.
    发明申请
    Fluorescent polypeptide complex 有权
    荧光多肽复合物

    公开(公告)号：US20070265430A1

    公开(公告)日：2007-11-15

    申请号：US11538940

    申请日：2006-10-05

    申请人： Albert Geiger ,  Hans Hornauer ,  Alfons Nichti ,  Peter Sluka

    发明人： Albert Geiger ,  Hans Hornauer ,  Alfons Nichti ,  Peter Sluka

    IPC分类号： C07K14/00

    CPC分类号： G01N33/533 ,  C07K14/43595 ,  G01N33/582 ,  Y10S977/705

    摘要： The present invention relates to a method of producing a fluorescent polypeptide complex characterized in that an isolated fluorescent polypeptide is inter-molecularly cross-linked with itself or one or more other polypeptides and wherein said complex is of 40 nm to 500 nm in size, it also relates to a complex obtainable by such method, to conjugates comprising such a fluorescent polypeptide complex and to the use of such complex or conjugate.

    摘要翻译： 本发明涉及一种生产荧光多肽复合物的方法，其特征在于分离的荧光多肽与其自身或一种或多种其他多肽分子内交联，其中所述复合物的大小为40nm至500nm， 还涉及通过这种方法获得的复合物，包含这种荧光多肽复合物的缀合物和这种复合物或缀合物的用途。

    Electrochemical sensor containing an enzyme linked to binding molecules bound to a noble metal surface
    8.
    发明授权
    Electrochemical sensor containing an enzyme linked to binding molecules bound to a noble metal surface 失效
    含有与结合到贵金属表面的结合分子相连的酶的电化学传感器

    公开(公告)号：US5834224A

    公开(公告)日：1998-11-10

    申请号：US519300

    申请日：1995-08-23

    申请人： Petra Ruger ,  Dorothee Ambrosius ,  Bernd Schmidt ,  Peter Sluka ,  Hans-Joachim Guder ,  Erhard Kopetzki

    发明人： Petra Ruger ,  Dorothee Ambrosius ,  Bernd Schmidt ,  Peter Sluka ,  Hans-Joachim Guder ,  Erhard Kopetzki

    IPC分类号： G01N27/327 ,  C07C323/58 ,  C07C323/60 ,  C07D207/323 ,  C07D207/44 ,  C07D207/452 ,  C07D403/12 ,  C07D495/04 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00 ,  C12Q1/54 ,  C12M1/34 ,  C12N11/14

    CPC分类号： C07D207/452 ,  B82Y15/00 ,  B82Y30/00 ,  C07C323/58 ,  C12Q1/003 ,  G01N33/54353 ,  Y10S435/817

    摘要： An electrochemical sensor is provided containing a supporting material having a surface of noble metal such as gold or palladium to which is bound an enzyme such as glucose oxidase via binding molecules. The binding molecules are in the form of a homogenous monolayer and are bound to the metal surface by anchor groups such as thiol, disulphide or phosphine groups. The enzyme is linked to the binding molecules by an ionic bond, a covalent bond or a metal-chelate bond. The binding molecules may contain a spacer such as an alkylene chain. Coverage of the binding molecules on the metal surface is less than the maximum coverage to optimize enzyme coverage, decrease response time of the sensor and increase signal yield per surface area. The monolayer may contain diluent molecules which are not linked to an enzyme and which are bound to the metal surface via anchor groups. The diluent molecules lower the concentration of binding molecules in the monolayer, increase conductivity of the monolayer and lower unspecific binding of components from a sample solution in contact with the sensor. The binding molecules can be linked by an ionic bond or a metal-chelate bond, respectively, to a charged amino acid residue or a chelate-forming amino acid residue of the N-terminus, C-terminus, or N- and C-terminus of recombinant enzyme protein.

    摘要翻译： 提供了一种电化学传感器，其包含具有贵金属如金或钯的表面的支撑材料，其通过结合分子结合酶如葡萄糖氧化酶。 结合分子是均匀单层的形式，并通过锚定基团例如硫醇，二硫键或膦基与金属表面结合。 酶通过离子键，共价键或金属螯合键与结合分子连接。 结合分子可以含有间隔基如亚烷基链。 金属表面上的结合分子的覆盖率小于最大覆盖范围，以优化酶覆盖，减少传感器的响应时间并增加每个表面积的信号产量。 单层可以含有不与酶连接并且通过锚定基团结合到金属表面的稀释剂分子。 稀释剂分子降低单层中结合分子的浓度，增加单层的电导率，并降低组件与传感器接触的样品溶液的非特异性结合。 结合分子可以分别通过离子键或金属 - 螯合键连接到带电荷的氨基酸残基或N-末端，C-末端或N-和C-末端的螯合形成氨基酸残基 的重组酶蛋白。