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    POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME
    4.
    发明申请
    POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME 有权
    可在无细胞翻译系统中显示FAB的多核苷酸结构，以及使用其制造和筛选FAB的方法

    公开(公告)号：US20130288908A1

    公开(公告)日：2013-10-31

    申请号：US13990654

    申请日：2011-11-30

    申请人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

    发明人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6854 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/55 ,  C12N15/1041 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/6857

    摘要： The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself, and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.

    摘要翻译： 以下的（1）或（2）的多核苷酸构建体用于进行核糖体展示，CIS显示和/或mRNA显示，以便针对感兴趣的抗原筛选Fab：（1）单核细胞系包含核糖体 - 结合序列，Fab第一链编码序列，接头肽编码序列，Fab第二链编码序列和支架编码序列，并且在其3'末端还包含与Fab保持复合物所必需的结构 其自身编码，和（2）包含Fab第一链表达顺反子和Fab第二链表达顺反子的多核苷酸构建体，其各自含有核糖体结合序列，Fab第一链编码序列或Fab第二链编码序列 和支架编码序列，所述第一Fab表达顺反子在其3'末端进一步包含核糖体失速序列，所述Fab第二链表达顺反子在其3'末端还包含结构ne 维持与Fab自身编码的复合物的塞车。

    Polymerization apparatus of producing vinyl chloride type polymer
    6.
    发明授权
    Polymerization apparatus of producing vinyl chloride type polymer 失效
    生产氯乙烯型聚合物的聚合装置

    公开(公告)号：US5750079A

    公开(公告)日：1998-05-12

    申请号：US758022

    申请日：1996-11-27

    申请人： Takuya Ueda ,  Kikuo Nakajima ,  Yoichi Tanifuji ,  Tadashi Amano ,  Shuji Ohnishi

    发明人： Takuya Ueda ,  Kikuo Nakajima ,  Yoichi Tanifuji ,  Tadashi Amano ,  Shuji Ohnishi

    IPC分类号： B01J19/00 ,  B01J19/18 ,  C08F2/04 ,  C08F2/18 ,  C08F14/06

    CPC分类号： B01J19/18 ,  B01J19/0013 ,  C08F14/06 ,  B01J2219/00081 ,  B01J2219/185 ,  B01J2219/1943

    摘要： A polymerization apparatus used, particularly, in suspension polymerization of vinyl chloride, comprising a polymerization vessel (1) having a substantially cylindrical inner space, said inner space of said polymerization vessel being provided with a plurality of baffles (5) comprising vertically extending pipes, and serpentine pipelines (6) each of which is placed between two adjacent baffles and is extended in a serpentine fashion along the polymerization vessel inner wall from the lower part to the upper part of the polymerization vessel, and a refrigerant being passed through said baffles and said serpentine pipelines. High-cooling performance can be obtained, a high-speed reaction in a large-sized polymerization vessel becomes possible, and a stable polymerization reaction can be carried out. A high-quality and uniform polymer that has few fish eyes and has a high bulk specific gravity can be obtained.

    摘要翻译： 一种聚合装置，特别是在氯乙烯的悬浮聚合中使用的聚合装置，包括具有基本上圆柱形的内部空间的聚合容器（1），所述聚合容器的所述内部空间设置有多个包括垂直延伸的管道的挡板（5） 和蛇形管道（6），每个管道布置在两个相邻的挡板之间，并且沿着聚合容器内壁从聚合容器的下部延伸到上部，并且制冷剂通过所述挡板和 说蛇形管道。 可以获得高冷却性能，可以在大型聚合容器中进行高速反应，并且可以进行稳定的聚合反应。 可以获得鱼眼少且体积比重高的高质量且均匀的聚合物。

    Vinyl chloride polymerization with recycle return projecting into gas phase region of reactor
    9.
    发明授权
    Vinyl chloride polymerization with recycle return projecting into gas phase region of reactor 失效
    乙烯基氯化物聚合反应再循环回到反应器的气相区域

    公开(公告)号：US5447999A

    公开(公告)日：1995-09-05

    申请号：US193866

    申请日：1994-07-09

    申请人： Takuya Ueda ,  Yoshihiro Shirota ,  Yoshihiko Hirai ,  Toshiaki Maruyama ,  Tadashi Amano

    发明人： Takuya Ueda ,  Yoshihiro Shirota ,  Yoshihiko Hirai ,  Toshiaki Maruyama ,  Tadashi Amano

    IPC分类号： B01J19/00 ,  C08F2/00 ,  C08F2/16 ,  C08F14/06 ,  C08F114/06

    CPC分类号： C08F2/002 ,  B01J19/002 ,  C08F14/06 ,  B01J2219/00252

    摘要： A polymerization process, comprises a polymerization vessel, a reflux condenser installed outside the polymerization vessel and a pipe connected between the reflux condenser and a wall of the polymerization vessel with an end thereof opening into a gaseous phase region inside the polymerization vessel, for returning the liquid condensate to the polymerization vessel, wherein said end projects from an inner surface of the wall of the polymerization vessel into the gaseous phase region. In polymerizing a monomer having an ethylenically unsaturated double bond using the polymerization apparatus, the quantity of heat removed by the reflux condenser is increased stepwise or continuously with progress of the polymerization, such that not less than 20% of the total reaction heat generated during the whole course of the polymerization is removed by the reflux condenser. With 100 or more repeated polymerization runs, polymer scale deposition inside the vessel is minimal even below the pipe end.

    摘要翻译： 聚合方法包括聚合容器，安装在聚合容器外部的回流冷凝器和连接在回流冷凝器和聚合容器壁之间的管道，其端部开口到聚合容器内的气相区域， 液体冷凝物到聚合容器中，其中所述端部从聚合容器的壁的内表面突出到气相区域中。 在使用聚合装置聚合具有烯键式不饱和双键的单体时，由回流冷凝器除去的热量随着聚合的进行而逐步或连续地增加，使得不少于20℃时产生的反应热总量 通过回流冷凝器除去整个聚合过程。 通过100次或更多次的反复聚合运行，在管端下方，容器内的聚合物垢沉积是最小的。