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    ANALYSIS METHOD AND ANALYZER
    3.
    发明申请
    ANALYSIS METHOD AND ANALYZER 审中-公开

    公开(公告)号：US20180180607A1

    公开(公告)日：2018-06-28

    申请号：US15854281

    申请日：2017-12-26

    申请人： SYSMEX CORPORATION

    发明人： Takuya KUBO

    IPC分类号： G01N33/543 ,  G01N35/00

    CPC分类号： G01N33/54326 ,  G01N35/00722 ,  G01N35/0098 ,  G01N2035/00564

    摘要： Disclosed is an analysis method including: a component to be analyzed being set; bringing a carrier and a complex that includes the component into contact with each other, to form the complex on the carrier; selecting a washing process in accordance with the component; and analyzing the component, with use of the washing process selected in accordance with the set component.

    SAMPLE ANALYZING METHOD AND SAMPLE ANALYZER
    5.
    发明申请
    SAMPLE ANALYZING METHOD AND SAMPLE ANALYZER 审中-公开

    公开(公告)号：US20200095631A1

    公开(公告)日：2020-03-26

    申请号：US16658190

    申请日：2019-10-21

    申请人： SYSMEX CORPORATION

    发明人： Takuya KUBO ,  Shigeki IWANAGA

    IPC分类号： C12Q1/6841 ,  G01N21/64

    摘要： A sample analyzing method includes: denaturing DNA by heating a measurement specimen; bleaching the measurement specimen to inhibit autofluorescence from the measurement specimen; binding a fluorescent dye to a test substance in the measurement specimen; and capturing an image of fluorescence originated from the fluorescent dye by irradiating the measurement specimen with light. The DNA denaturation treatment is performed before the bleaching.

    SAMPLE ANALYZING METHOD AND SAMPLE ANALYZER
    7.
    发明申请
    SAMPLE ANALYZING METHOD AND SAMPLE ANALYZER 审中-公开
    样品分析方法和样品分析仪

    公开(公告)号：US20160281151A1

    公开(公告)日：2016-09-29

    申请号：US15075358

    申请日：2016-03-21

    申请人： SYSMEX CORPORATION

    发明人： Takuya KUBO ,  Shigeki IWANAGA

    IPC分类号： C12Q1/68

    摘要： A sample analyzing method includes: denaturing DNA by heating a measurement specimen; bleaching the measurement specimen to inhibit autofluorescence from the measurement specimen; binding a fluorescent dye to a test substance in the measurement specimen; and capturing an image of fluorescence originated from the fluorescent dye by irradiating the measurement specimen with light. The DNA denaturation treatment is performed before the bleaching.

    摘要翻译： 样品分析方法包括：通过加热测量样品来变性DNA; 漂白测量样品以抑制测量样品的自发荧光; 将荧光染料与测量样品中的测试物质结合; 以及通过用光照射测量样品来捕获来自荧光染料的荧光的图像。 脱色前进行DNA变性处理。

    CELL ANALYZER, CELL ANALYZER CONTROLLING METHOD, AND PROGRAM
    9.
    发明申请
    CELL ANALYZER, CELL ANALYZER CONTROLLING METHOD, AND PROGRAM 审中-公开

    公开(公告)号：US20170362553A1

    公开(公告)日：2017-12-21

    申请号：US15695633

    申请日：2017-09-05

    申请人： Sysmex Corporation

    发明人： Kanako MASUMOTO ,  Takuya KUBO ,  Shigeki IWANAGA ,  Masaya OKADA

    IPC分类号： C12M1/34 ,  G01N21/64 ,  G01N33/50 ,  G06T7/00

    CPC分类号： C12M1/34 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N2021/6432 ,  G01N2021/6441 ,  G06K9/00134 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/30072

    摘要： Provided is a cell analyzer including: a light source unit configured to apply light to test cells each containing first substances which are bound to first fluorescent dyes and which serve as an index for therapeutic strategy judgement; an image capturing unit configured to capture an image of fluorescence caused by the light; a processing unit configured to process the image obtained by the image capturing unit; and a display unit configured to display a process result obtained by the processing unit, wherein the processing unit obtains a first image by performing an inactivation process of quenching the first fluorescent dyes, an activation process of activating a part of the first fluorescent dyes that have been quenched, and an image capturing process of capturing, by means of the image capturing unit, an image of the fluorescence by applying light from the light source unit to each test cell; extracts bright points based on the first fluorescent dyes on the basis of the first image; classifies the extracted bright points into groups each corresponding to one first substance, thereby to obtain the number of the first substances in the test cell on the basis of the number of the classified groups; obtains therapy index information serving as an index for therapeutic strategy judgement, on the basis of the obtained number of the first substances; and causes the display unit to display the obtained therapy index information.

    CELL ANALYSIS METHOD
    10.
    发明申请
    CELL ANALYSIS METHOD 审中-公开
    细胞分析方法

    公开(公告)号：US20150276574A1

    公开(公告)日：2015-10-01

    申请号：US14661020

    申请日：2015-03-18

    申请人： SYSMEX CORPORATION

    发明人： Maria KIRIYAMA ,  Takuya KUBO

    IPC分类号： G01N15/14 ,  G01N1/30

    CPC分类号： G01N15/14 ,  G01N1/30 ,  G01N1/36 ,  G01N1/4044 ,  G01N1/44 ,  G01N15/1459 ,  G01N33/5306 ,  G01N2001/366 ,  G01N2015/1006

    摘要： A cell analysis method is provided that comprises (a) removing embedding medium from a sample obtained from the tissue specimen which is aldehyde-fixed and embedded in the water-insoluble embedding medium, thereby obtaining the sample comprising the tissue from which the embedding medium is removed; (b) heating the sample obtained from step (a) in the presence of a divalent carboxylic acid compound to obtain a heat-treated sample comprising the tissue; (c) contacting the heat-treated sample from step (b) with an enzyme having a cell dispersion activity to separate the sample into individual cells, thereby obtaining the sample comprising the individual cells dispersed in a solvent; (d) subjecting the separated cells from step (c) to flow cytometry to obtain optical information; and (e) analyzing the cells based on the optical information obtained in the step (d).

    摘要翻译： 提供了一种细胞分析方法，其包括（a）从醛固定并嵌入在水不溶性包埋介质中的组织标本获得的样品中除去包埋培养基，从而获得包含组织的样品，其中嵌入介质为 删除 （b）在二元羧酸化合物的存在下加热步骤（a）得到的样品，得到包含组织的热处理样品; （c）将来自步骤（b）的经热处理的样品与具有细胞分散活性的酶接触以将样品分离成单个细胞，从而获得包含分散在溶剂中的各个细胞的样品; （d）使来自步骤（c）的分离的细胞流式细胞仪获得光学信息; 和（e）基于步骤（d）中获得的光学信息来分析单元。