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    Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies
    1.
    发明申请
    Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies 审中-公开
    用于快速检测犬细小病毒抗原和抗体的简单测试

    公开(公告)号：US20100137159A1

    公开(公告)日：2010-06-03

    申请号：US12606451

    申请日：2009-10-27

    申请人： Sanjay Kapil ,  Shashidhara Marulappa

    发明人： Sanjay Kapil ,  Shashidhara Marulappa

    IPC分类号： C12Q1/70 ,  C40B30/04

    CPC分类号： G01N33/56983 ,  C12N2750/14311 ,  G01N2333/015 ,  G01N2469/20

    摘要： Slide agglutination tests (SATs) and slide inhibition tests (SITs) provide rapid detection, quantitation and strain identification of red blood cell (RBC) agglutinating viruses such as canine parvovirus (CPV) in biological samples. The tests are rapid, low-cost, and easy to use. These tests do not require any expensive equipment and can thus be used to monitor infections and antibody titers under field conditions. The tests can be modified to detect results using fluorescence (FSAT). FSAT is useful for rapid high-throughput screening (HTS) of libraries of small molecules and/or chemical compounds to identify antiviral compounds useful for the treatment of diseases caused by emerging hemaglutinating viruses that infect animals and humans.

    摘要翻译： 幻灯片凝集试验（SAT）和幻灯片抑制试验（SITs）可提供生物样品中红细胞（RBC）凝集病毒如犬细小病毒（CPV）的快速检测，定量和毒株鉴定。 测试速度快，成本低，易于使用。 这些测试不需要任何昂贵的设备，因此可用于监测现场条件下的感染和抗体滴度。 可以修改测试以使用荧光（FSAT）检测结果。 FSAT可用于小分子和/或化学化合物文库的快速高通量筛选（HTS），以鉴定可用于治疗由感染动物和人类的新出现的血液流感病毒引起的疾病的抗病毒化合物。

    Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding
    2.
    发明授权
    Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding 失效
    鉴定和应用猪繁殖与呼吸综合征病毒宿主易感因子，改善猪繁殖

    公开(公告)号：US06740490B2

    公开(公告)日：2004-05-25

    申请号：US09772044

    申请日：2001-01-29

    申请人： Sanjay Kapil ,  Kumar Shanmukhappa

    发明人： Sanjay Kapil ,  Kumar Shanmukhappa

    IPC分类号： C12Q168

    CPC分类号： C12N7/00 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/02 ,  A61K39/00 ,  C07K14/70596 ,  C12N2770/38011 ,  C12N2770/38022 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/56983 ,  G01N33/6872 ,  G01N2333/70596

    摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious economic losses in swine. The present invention determined that CD 151 is a susceptibility factor for PRRSV infection by transfecting a cell line which is not susceptible to PRRSV infection (BHK-21) with CD 151, which rendered the cell line susceptible. Because CD 151 can be accessed in cellular material including blood platelets and germplasm, the present invention provides a non-invasive method of screening different swine for susceptibility to PRRSV, thereby improving breeding plans. In the case of a valuable animal, results from such screening can indicate any offspring's susceptibility to PPRSV. Additionally, the viral RNA-CD 151 interaction possesses high affinity and can be used as a tool to detect anti-viral compounds which can be further improved by using combinatorial chemistry. Accordingly, anti-viral compounds that can block the viral RNA-CD 151 interaction can be developed. Advantageously, transfection of CD 151 into non-simian cell lines can confer susceptibility to PRRSV and these recombinant cell lines can be used for preparation of biologics that will avoid simian cell lines which could be a source of primate viruses in xenotransplanted organs from pigs. Finally, the present invention describes the basic mechanism by which the virus RNA enters a target cell. This novel class of proteins is termed viral RNA entry proteins and a novel class of compounds named anti-RNA Entry Proteins can be used to block the entry of viral RNA, thereby preventing viral infections.

    摘要翻译： 猪繁殖与呼吸综合征病毒（PRRSV）导致猪严重经济损失。 本发明通过用CD151转染对PRRSV感染（BHK-21）不敏感的细胞系（其使细胞系易感）确定CD151是PRRSV感染的易感因子。 由于CD 151可以在包括血小板和种质的细胞材料中进行，所以本发明提供了对PRRSV易感性的不同猪筛选的非侵入性方法，从而改善育种计划。 在有价值的动物的情况下，这种筛选的结果可以指示任何后代对PPRSV的易感性。 此外，病毒RNA-CD 151相互作用具有高亲和力，可用作检测抗病毒化合物的工具，可通过组合化学进一步改善。 因此，可以开发可以阻断病毒RNA-CD 151相互作用的抗病毒化合物。 有利地，将CD 151转染到非猿猴细胞系中可以赋予PRRSV的易感性，并且这些重组细胞系可用于制备生物制剂，其将避免可能是来自猪的异种移植器官中的灵长类动物病毒源的猿猴细胞系。 最后，本发明描述了病毒RNA进入靶细胞的基本机制。 这种新型的蛋白质类型被称为病毒RNA进入蛋白质，一类名为抗RNA进入蛋白质的化合物可用于阻断病毒RNA的进入，从而防止病毒感染。

    Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding and development of a non-simian recombinant cell line for propagation of the virus and a target for a novel class of antiviral compounds
    4.
    发明授权
    Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding and development of a non-simian recombinant cell line for propagation of the virus and a target for a novel class of antiviral compounds 失效
    识别和应用猪繁殖与呼吸综合征病毒宿主易感性因子，用于改善猪繁殖和开发用于病毒繁殖的非猿类重组细胞系和新型抗病毒化合物的靶标

    公开(公告)号：US07175984B2

    公开(公告)日：2007-02-13

    申请号：US10058597

    申请日：2002-01-28

    申请人： Sanjay Kapil ,  Kumar Shanmukhappa

    发明人： Sanjay Kapil ,  Kumar Shanmukhappa

    IPC分类号： C12Q1/68 ,  C12N1/20 ,  C12N5/00 ,  C07H19/00

    CPC分类号： C12N7/00 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/02 ,  A61K39/00 ,  C07K14/70596 ,  C12N2770/38011 ,  C12N2770/38022 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/56983 ,  G01N33/6872 ,  G01N2333/70596

    摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious economic losses in swine. The present invention determined that CD 151 is a susceptibility factor for PRRSV infection by transfecting a cell line which is not susceptible to PRRSV infection (BHK-21) with CD 151, which rendered the cell line susceptible. Because CD 151 can be accessed in cellular material including blood platelets and germplasm, the present invention provides a non-invasive method of screening different swine for susceptibility to PRRSV, thereby improving breeding plans. In the case of a valuable animal, results from such screening can indicate any offspring's susceptibility to PPRSV. Additionally, the viral RNA-CD 151 interaction possesses high affinity and can be used as a tool to detect anti-viral compounds which can be further improved by using combinatorial chemistry. Accordingly, anti-viral compounds that can block the viral RNA-CD 151 interaction can be developed. Advantageously, transfection of CD 151 into non-simian cell lines can confer susceptibility to PRRSV and these recombinant cell lines can be used for preparation of biologics that will avoid simian cell lines which could be a source of primate viruses in xenotransplanted organs from pigs. Finally, the present invention describes the basic mechanism by which the virus RNA enters a target cell. This novel class of proteins is termed viral RNA entry proteins and a novel class of compounds named anti-RNA Entry Proteins can be used to block the entry of viral RNA, thereby preventing viral infections.

    摘要翻译： 猪繁殖与呼吸综合征病毒（PRRSV）导致猪严重经济损失。 本发明通过用CD151转染对PRRSV感染（BHK-21）不敏感的细胞系（其使细胞系易感）确定CD151是PRRSV感染的易感因子。 由于CD 151可以在包括血小板和种质的细胞材料中进行，所以本发明提供了对PRRSV易感性的不同猪筛选的非侵入性方法，从而改善育种计划。 在有价值的动物的情况下，这种筛选的结果可以指示任何后代对PPRSV的易感性。 此外，病毒RNA-CD 151相互作用具有高亲和力，可用作检测抗病毒化合物的工具，可通过组合化学进一步改善。 因此，可以开发可以阻断病毒RNA-CD 151相互作用的抗病毒化合物。 有利地，将CD 151转染到非猿猴细胞系中可以赋予PRRSV的易感性，并且这些重组细胞系可用于制备生物制剂，其将避免可能是来自猪的异种移植器官中的灵长类动物病毒源的猿猴细胞系。 最后，本发明描述了病毒RNA进入靶细胞的基本机制。 这种新型的蛋白质类型被称为病毒RNA进入蛋白质，一类名为抗RNA进入蛋白质的化合物可用于阻断病毒RNA的进入，从而防止病毒感染。

    SUPRALINGUAL VACCINES AND APPLICATORS
    6.
    发明申请
    SUPRALINGUAL VACCINES AND APPLICATORS 审中-公开
    非疫苗和应用者

    公开(公告)号：US20120121645A1

    公开(公告)日：2012-05-17

    申请号：US13384505

    申请日：2010-07-15

    申请人： Sanjay Kapil

    发明人： Sanjay Kapil

    IPC分类号： A61K39/23 ,  A61K39/12 ,  A61K39/135 ,  A61J7/00 ,  A61P37/04 ,  A61P31/20 ,  A61P31/14 ,  A61K39/00 ,  A61K39/125

    CPC分类号： A61K39/23 ,  A61K9/0056 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/541 ,  A61K2039/542 ,  A61K2039/55 ,  A61K2039/552 ,  C12N2750/14334

    摘要： Solid and semi-solid formulations are used for supralingual administration of vaccines to animals. The formulations, which comprise antigens dispersed in a solid or semi-solid matrix, or paste, are delivered via supralingual applicators. The supralingual applicators are designed so as to position the antigen-containing matrix directly on the dorsal surface of the tongue during vaccine delivery. Upon exposure to saliva and to suckling and/or licking action of the tongue, the matrix dissolves and releases antigens to the tongue. In some embodiments, the antigens are viruses, for example, attenuated viruses that are capable of infecting cells of the tongue, e.g. canine parvoviruses which infect basal tongue cells. The supralingual applicators are especially useful for the delivery of vaccines to newborn animals.

    摘要翻译： 固体和半固体制剂用于对动物进行疫苗的上服给药。 包含分散在固体或半固体基质中的抗原或糊剂的制剂通过中间施用器递送。 设计上呼吸器，以便在疫苗输送期间将含抗原的基质直接定位在舌的背表面上。 暴露于唾液和舌头的乳和/或舔动作用时，基质溶解并释放抗原到舌头。 在一些实施方案中，抗原是病毒，例如能够感染舌头细胞的减毒病毒，例如， 感染基底细胞的犬细小病毒。 上呼吸器特别适用于将疫苗运送到新生儿。

    Multivalent bovine coronavirus vaccine and method of treating bovine coronavirus infection
    8.
    发明授权
    Multivalent bovine coronavirus vaccine and method of treating bovine coronavirus infection 失效
    多价牛冠状病毒疫苗及治疗牛冠状病毒感染的方法

    公开(公告)号：US5916570A

    公开(公告)日：1999-06-29

    申请号：US795294

    申请日：1997-02-04

    申请人： Sanjay Kapil

    发明人： Sanjay Kapil

    IPC分类号： A61K39/215 ,  A61P31/12 ,  C12N7/00 ,  C12N7/04 ,  C12N15/11 ,  C12N15/15

    CPC分类号： A61K31/7048 ,  A61K39/12 ,  A61K39/215 ,  C12N7/00 ,  A61K2039/5254 ,  A61K2039/543 ,  A61K2039/552 ,  A61K2039/70 ,  C12N2770/20034 ,  Y10S530/826

    摘要： New purified and isolated bovine coronavirus (BCV) types (II and III) are described which can be used to create new modified live vaccines for administration to cattle in order to confer immunity against virulent wild-type bovine coronavirus infection. Preferably, a multivalent modified live vaccine is provided for oral-nasal administration which includes the known Type I virus and the new Types II and III virus. Hygromycin B has also demonstrated to be effective for suppressing BCV replication and thus can be administered to cattle as a treatment for the chronic disease and to suppress shedding of BCV in cattle feces.

    摘要翻译： 描述了新的纯化和分离的牛冠状病毒（BCV）类型（II和III），其可用于产生用于施用牛的新的修饰活疫苗，以赋予对强毒野生型牛冠状病毒感染的免疫力。 优选地，提供用于口服鼻内给药的多价改性活疫苗，其包括已知的I型病毒和新的II型和III型病毒。 潮霉素B也被证明对抑制BCV复制是有效的，因此可以作为慢性疾病的治疗而施用于牛，并抑制牛粪中BCV的脱落。