公开(公告)号：US20130316892A1

公开(公告)日：2013-11-28

申请号：US13994495

申请日：2011-12-14

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

IPC分类号： B01J20/281

CPC分类号： B01J20/281 ,  B01J20/267 ,  B01J20/285 ,  C08B1/003 ,  C08B15/10 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08J3/16 ,  C08J3/24 ,  C08J2301/02 ,  C08K5/0025 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  Y02P20/542

摘要： Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

摘要翻译： 提供当用作色谱填料时可以以高流速使用的聚合物颗粒，即具有适合于大量加工的优异的电阻流速，并且还具有对蛋白质等靶分子的高结合力 粒子中含有适当的配体，以及聚合物粒子的制造方法。 特别是交联聚合物颗粒和交联聚合物颗粒的制备方法，多糖复合颗粒和多糖复合颗粒的制备方法，使用该聚合物颗粒的色谱用填料和用于抗体纯化的吸附剂。 本发明涉及A.一种多糖复合粒子的制造方法，其特征在于，包括以下工序（1）〜（3）：（1）制备多糖溶液的工序，其中将2种以上的多糖溶解于 离子液体 （2）将多糖溶液的液滴分散在与离子液体的相容性低的有机溶剂中的多糖溶液的液滴分散液的制备工序; 和（3）将多糖的复合体凝结而得到多糖复合粒子的凝固工序; 和B.一种制备交联聚合物颗粒的方法，该方法包括使溶解在离子液体中的聚合物与交联剂反应的步骤，同时聚合物在与 离子液体。

公开(公告)号：US09352298B2

公开(公告)日：2016-05-31

申请号：US13994495

申请日：2011-12-14

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

IPC分类号： B01J20/22 ,  B01J20/281 ,  B01J20/285 ,  C08J3/16 ,  C08J3/24 ,  C08B1/00 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  C08B15/10 ,  C08B37/00 ,  C08B37/02 ,  C08B37/08 ,  B01J20/26

CPC分类号： B01J20/281 ,  B01J20/267 ,  B01J20/285 ,  C08B1/003 ,  C08B15/10 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08J3/16 ,  C08J3/24 ,  C08J2301/02 ,  C08K5/0025 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  Y02P20/542

摘要： Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

摘要翻译： 提供当用作色谱填料时可以以高流速使用的聚合物颗粒，即具有适合于大量加工的优异的电阻流速，并且还具有对蛋白质等靶分子的高结合力 粒子中含有适当的配体，以及聚合物粒子的制造方法。 特别是交联聚合物颗粒和交联聚合物颗粒的制备方法，多糖复合颗粒和多糖复合颗粒的制备方法，使用该聚合物颗粒的色谱用填料和用于抗体纯化的吸附剂。 本发明涉及A.一种多糖复合粒子的制造方法，其特征在于，包括以下工序（1）〜（3）：（1）制备多糖溶液的工序，其中将2种以上的多糖溶解于 离子液体 （2）将多糖溶液的液滴分散在与离子液体的相容性低的有机溶剂中的多糖溶液的液滴分散液的制备工序; 和（3）将多糖的复合体凝结而得到多糖复合粒子的凝固工序; 和B.一种制备交联聚合物颗粒的方法，该方法包括使溶解在离子液体中的聚合物与交联剂反应的步骤，同时聚合物在与 离子液体。

公开(公告)号：US09162161B2

公开(公告)日：2015-10-20

申请号：US13638751

申请日：2011-03-29

申请人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

发明人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

IPC分类号： B01D15/04 ,  B01D15/08 ,  B01D15/38 ,  C08F8/00 ,  C08F220/26 ,  B01J20/26 ,  B01J20/286

CPC分类号： B01D15/3804 ,  B01J20/265 ,  B01J20/286 ,  C08F8/00 ,  C08F220/26 ,  Y10T428/2982

摘要： Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

摘要翻译： 本发明提供一种对蛋白质具有高动态结合能力并具有优异的耐碱性和储存稳定性的亲和层析用填料。 本发明的亲和层析用填料包含由40〜99.5质量份的含有甲基丙烯酰基的含有甲基丙烯酰基的乙烯基单体（不含有环氧基）的（M-1）共聚物构成的多孔质粒子 ，（M-2）含有环氧基的乙烯基单体0.5〜30质量份，除了单体（M）以外的（M-3）含有甲基丙烯酰基的乙烯基单体的（M-3） -1）和（M-2）和0〜25质量份（M-4）除了单体（M-1），（M-2）和（M-3） 条件是（M-1），（M-2），（M-3）和（M-4）的含量的总量为100质量份） 通过共聚物中包含的环氧基开环获得的开环环氧基; 和与多孔颗粒结合的配体。

公开(公告)号：US20130085199A1

公开(公告)日：2013-04-04

申请号：US13638751

申请日：2011-03-29

申请人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

发明人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

IPC分类号： C08F220/00 ,  C07K1/22 ,  C08F8/00

CPC分类号： B01D15/3804 ,  B01J20/265 ,  B01J20/286 ,  C08F8/00 ,  C08F220/26 ,  Y10T428/2982

摘要： Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

摘要翻译： 本发明提供一种对蛋白质具有高动态结合能力并具有优异的耐碱性和储存稳定性的亲和层析用填料。 本发明的亲和层析用填料包含由40〜99.5质量份的含有甲基丙烯酰基的含有甲基丙烯酰基的乙烯基单体（不含有环氧基）的（M-1）共聚物构成的多孔质粒子 ，（M-2）含有环氧基的乙烯基单体0.5〜30质量份，除了单体（M）以外的（M-3）含有甲基丙烯酰基的乙烯基单体的（M-3） -1）和（M-2）和0〜25质量份（M-4）除了单体（M-1），（M-2）和（M-3） 条件是（M-1），（M-2），（M-3）和（M-4）的含量的总量为100质量份） 通过共聚物中包含的环氧基开环获得的开环环氧基; 和与多孔颗粒结合的配体。