Method for producing tissue formation and tissue culture kit
    3.
    发明授权
    Method for producing tissue formation and tissue culture kit 失效
    组织形成和组织培养试剂盒的制备方法

    公开(公告)号:US6051425A

    公开(公告)日:2000-04-18

    申请号:US858905

    申请日:1997-05-19

    摘要: A matrix for tissue culture comprising two kinds of sponges having at least one different physical property and/or at least one different chemical property; a method for culturing tissue using said matrix for tissue culture comprising inoculating and culturing first cell on a first sponge, laminating a second sponge thereon, and inoculating and culturing second cell on said second sponge; a method for fixing a cultured tissue comprising placing a cultured tissue in gelatin solution solated by elevating temperature, lowering temperature to gelatinize gelatin to fix the cultured tissue by said gelatinated gelatin; and an artificial skin fixed comprising dermis layer fixed by gelatin and epidermis layer laminated on the dermis layer.

    摘要翻译: 一种用于组织培养的基质,其包含具有至少一种不同物理性质和/或至少一种不同化学性质的两种海绵; 使用所述用于组织培养的基质培养组织的方法,包括在第一海绵上接种和培养第一细胞,在其上层叠第二海绵,以及在所述第二海绵上接种和培养第二细胞; 一种固定培养组织的方法,包括将培养的组织置于通过升温溶解的明胶溶液中,降低温度以使明胶糊化以通过所述明胶明胶固定培养的组织; 以及由真皮层固定的明胶固定的人造皮肤和表层层叠在真皮层上。

    Method for fixing cultured tissue and artificial skin fixed
    5.
    发明授权
    Method for fixing cultured tissue and artificial skin fixed 有权
    固定培养组织和人造皮肤的方法

    公开(公告)号:US06300128B1

    公开(公告)日:2001-10-09

    申请号:US09479036

    申请日:2000-01-07

    IPC分类号: C12N500

    摘要: A matrix for tissue culture comprising two kinds of sponges having at least one different physical property and/or at least one different chemical property; a method for culturing tissue using said matrix for tissue culture comprising inoculating and culturing first cell on a first sponge, laminating a second sponge thereon, and inoculating and culturing second cell on said second sponge; a method for fixing a cultured tissue comprising placing a cultured tissue in gelatin solution solated by elevating temperature, lowering temperature to gelatinize gelatin to fix the cultured tissue by said gelatinated gelatin; and an artificial skin fixed comprising dermis layer fixed by gelatin and epidermis layer laminated on the dermis layer.

    摘要翻译: 一种用于组织培养的基质,其包含具有至少一种不同物理性质和/或至少一种不同化学性质的两种海绵; 使用所述用于组织培养的基质培养组织的方法,包括在第一海绵上接种和培养第一细胞,在其上层叠第二海绵,以及在所述第二海绵上接种和培养第二细胞; 一种固定培养组织的方法,包括将培养的组织置于通过升温溶解的明胶溶液中,降低温度以使明胶糊化以通过所述明胶明胶固定培养的组织; 以及由真皮层固定的明胶固定的人造皮肤和表层层叠在真皮层上。