利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

65 条记录 (耗时 0.023 秒)

    Avian herpesvirus-based recombinant infectious bursal disease vaccine
    2.
    发明授权
    Avian herpesvirus-based recombinant infectious bursal disease vaccine 有权
    禽疱疹病毒重组传染性法氏囊病疫苗

    公开(公告)号：US06764684B2

    公开(公告)日：2004-07-20

    申请号：US09964895

    申请日：2001-09-28

    申请人： Shuji Saitoh ,  Takashi Okuda ,  Mayumi Kubomura ,  Kristi M. Moore

    发明人： Shuji Saitoh ,  Takashi Okuda ,  Mayumi Kubomura ,  Kristi M. Moore

    IPC分类号： A61K3912

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/55 ,  A61K2039/552 ,  C12N2710/16321 ,  C12N2720/10034 ,  Y10S424/816

    摘要： The present invention provides an avian recombinant herpesvirus modified by the presence of the cDNA encoding the VP2 of the Delaware Variant E strain of IBDV, a subtype of IBDV serotype 1 strains. The present invention further provides an avian recombinant herpesvirus comprised of the VP2 gene, of which the backbone virus is a Marek's disease vaccine strain, such as herpesvirus of turkeys. A poultry vaccine including the avian herpes recombinant virus described in the present invention can induce in chickens protective immunity against a variety of different subtypes of IBDV.

    摘要翻译： 本发明提供了通过编码IBDV的Delaware变体E株的IBVV亚型IBDV血清型1株的VP2的cDNA的存在而修饰的禽重组疱疹病毒。 本发明还提供了由VP2基因组成的禽类重组疱疹病毒，其中主要病毒是马立克氏病疫苗株，例如火鸡疱疹病毒。 包括本发明中描述的禽疱疹重组病毒的家禽疫苗可诱导针对IBDV的各种不同亚型的鸡的保护性免疫。

    SEMICONDUCTOR STORAGE DEVICE
    6.
    发明申请
    SEMICONDUCTOR STORAGE DEVICE 有权
    半导体存储设备

    公开(公告)号：US20130069132A1

    公开(公告)日：2013-03-21

    申请号：US13606472

    申请日：2012-09-07

    申请人： Tomoaki Atsumi ,  Takashi Okuda

    发明人： Tomoaki Atsumi ,  Takashi Okuda

    IPC分类号： H01L27/108 ,  H01L23/485

    CPC分类号： H01L27/112 ,  H01L21/76801 ,  H01L21/76826 ,  H01L27/0688 ,  H01L27/10805 ,  H01L27/1085 ,  H01L27/10873 ,  H01L27/10897 ,  H01L27/1156 ,  H01L27/1207 ,  H01L27/1225 ,  H01L29/16 ,  H01L29/78 ,  H01L29/7869

    摘要： Probability of malfunction of a semiconductor storage device is reduced. A shielding layer is provided between a memory cell array (e.g., a memory cell array including a transistor formed using an oxide semiconductor material) and a peripheral circuit (e.g., a peripheral circuit including a transistor formed using a semiconductor substrate), which are stacked. With this structure, the memory cell array and the peripheral circuit can be shielded from radiation noise generated between the memory cell array and the peripheral circuit. Thus, probability of malfunction of the semiconductor storage device can be reduced.

    摘要翻译： 减少了半导体存储装置故障的概率。 屏蔽层设置在堆叠的存储单元阵列（例如，包括使用氧化物半导体材料形成的晶体管的存储单元阵列）和周边电路（例如，包括使用半导体衬底形成的晶体管的外围电路）之间 。 利用这种结构，存储单元阵列和外围电路可以屏蔽在存储单元阵列和外围电路之间产生的辐射噪声。 因此，可以降低半导体存储装置的故障概率。

    Insect Desiccation Resistance Genes and Uses Thereof
    7.
    发明申请
    Insect Desiccation Resistance Genes and Uses Thereof 有权
    昆虫抗病基因及其用途

    公开(公告)号：US20110281349A1

    公开(公告)日：2011-11-17

    申请号：US13103806

    申请日：2011-05-09

    申请人： Takahiro Kikawada ,  Takashi Okuda ,  Masahiko Watanabe ,  Kazuei Mita ,  Keiko Kadono

    发明人： Takahiro Kikawada ,  Takashi Okuda ,  Masahiko Watanabe ,  Kazuei Mita ,  Keiko Kadono

    IPC分类号： C12N5/10 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C07K14/43563

    摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3.) When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA 1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.

    摘要翻译： 本发明的目的在于提供编码昆虫抗干扰性蛋白质的多核苷酸及其使用方法，从干燥状态的Polypedilum vanderplanki幼虫生产cDNA文库，构建P.vanderplanki EST数据库，分离编码LEA蛋白质的基因。 这导致了三种类型的编码LEA样蛋白的新基因（PvLEA1，PvLEA2和PvLEA3）的成功分离。当对从每个基因推导的蛋白进行二级结构预测和基序搜索时，所有三种蛋白都具有α 富含螺旋的结构和LEA_4基序，是LEA蛋白的特征。 此外，由PvLEA1,2和3基因合成的重组蛋白即使在沸腾时也是热溶性的，因此PvLEA1,2和3蛋白具有高亲水性以及植物LEA蛋白。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些PvLEA 1,2和3基因引入动物细胞成功地赋予细胞干燥抗性。 本发明提供了从昆虫分离的LEA基因的第一个实例。

    Semiconductor device
    8.
    发明授权
    Semiconductor device 有权
    半导体器件

    公开(公告)号：US07557427B2

    公开(公告)日：2009-07-07

    申请号：US11845339

    申请日：2007-08-27

    申请人： Takashi Okuda ,  Yasuo Morimoto ,  Yuko Maruyama ,  Toshio Kumamoto

    发明人： Takashi Okuda ,  Yasuo Morimoto ,  Yuko Maruyama ,  Toshio Kumamoto

    IPC分类号： H01L51/05

    CPC分类号： H01L23/5223 ,  H01L23/5225 ,  H01L2924/0002 ,  H01L2924/3011 ,  H01L2924/00

    摘要： A semiconductor device includes a semiconductor substrate including a main surface; a plurality of first interconnections formed in a capacitance forming region defined on the main surface and extending in a predetermined direction; a plurality of second interconnections each adjacent to the first interconnection located at an edge of the capacitance forming region, extending in the predetermined direction, and having a fixed potential; and an insulating layer formed on the main surface and filling in between each of the first interconnections and between the first interconnection and the second interconnection adjacent to each other. The first interconnections and the second interconnections are located at substantially equal intervals in a plane parallel to the main surface, and located to align in a direction substantially perpendicular to the predetermined direction.

    摘要翻译： 半导体器件包括：包括主表面的半导体衬底; 多个第一互连形成在形成在主表面上并沿预定方向延伸的电容形成区域中; 多个第二互连，每个相邻于位于电容形成区域的边缘处的第一互连，沿预定方向延伸并具有固定电位; 以及绝缘层，形成在主表面上，并且填充在每个第一互连之间以及第一互连和第二互连之间相邻。 第一互连和第二互连在平行于主表面的平面中以基本相等的间隔定位，并且被定位成在基本上垂直于预定方向的方向上对齐。

    Trehalose transporter gene and method of introducing trehalose into cells
    9.
    发明申请
    Trehalose transporter gene and method of introducing trehalose into cells 有权
    海藻糖转运蛋白基因和将海藻糖引入细胞的方法

    公开(公告)号：US20090111176A1

    公开(公告)日：2009-04-30

    申请号：US12073457

    申请日：2008-03-05

    申请人： Takahiro Kikawada ,  Takashi Okuda ,  Masahiko Watanabe ,  Ayako Saito ,  Yasushi Kanamori ,  Yuichi Nakahara

    发明人： Takahiro Kikawada ,  Takashi Okuda ,  Masahiko Watanabe ,  Ayako Saito ,  Yasushi Kanamori ,  Yuichi Nakahara

    IPC分类号： C12N5/06 ,  C12N15/11 ,  C07K14/00 ,  C12N15/00

    CPC分类号： C07K14/43563 ,  C07K14/43577

    摘要： There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.

    摘要翻译： 提供海藻糖转运蛋白基因和通过使用该基因将海藻糖引入细胞的方法。 在P. vanderplanki EST中搜索海藻糖转运蛋白基因的候选物，得到获得的命名为Tret1的cDNA。 Tret1编码具有12个跨膜结构的504个氨基酸的蛋白质。 Tret1表达由干燥应激诱导，在脂肪体中占优势。 非洲爪蟾卵母细胞中TRET1的功能表达显示，转运活性对海藻糖是特异性的，独立于细胞外pH和电化学膜电位。 根据海藻糖的浓度梯度，TRET1的转运方向是可逆的。 TRET1对于海藻糖的表观Km和Vmax是非常高的值。 这些结果表明TRET1是一种促进的高容量海藻糖特异性转运蛋白。 Tret1在昆虫中广泛存在。 此外，TRET1在包括脊椎动物以及昆虫的细胞中赋予海藻糖通透性。