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    Method for preparing unsaturated fatty acids
    1.
    发明申请
    Method for preparing unsaturated fatty acids 审中-公开
    不饱和脂肪酸的制备方法

    公开(公告)号：US20060229461A1

    公开(公告)日：2006-10-12

    申请号：US11388416

    申请日：2006-03-24

    申请人： Soon-Kee Sung ,  Jung Yi ,  Seong Lee

    发明人： Soon-Kee Sung ,  Jung Yi ,  Seong Lee

    IPC分类号： C07C51/43

    CPC分类号： C07C51/487 ,  C07C51/43 ,  C11C1/007 ,  C11C3/14 ,  C07C57/03 ,  C07C57/12

    摘要： Disclosed relates to a method for preparing unsaturated fatty acids and, more particularly, to a method for preparing unsaturated fatty acids in a high purity of at least 99% by isolating and purifying unsaturated fatty acids via a secondary nucleation mechanism using fatty acid-urea inclusion compounds.

    摘要翻译： 本发明涉及一种制备不饱和脂肪酸的方法，更具体地说，涉及通过使用脂肪酸 - 脲包合物通过二次成核机理分离和纯化不饱和脂肪酸，制备高纯度至少99％的不饱和脂肪酸的方法 化合物。

    Method for improving conversion rate of oil soluble unsaturated lipids into water-soluble lipids
    2.
    发明申请
    Method for improving conversion rate of oil soluble unsaturated lipids into water-soluble lipids 失效
    将油溶性不饱和脂质转化率提高为水溶性脂质的方法

    公开(公告)号：US20060228322A1

    公开(公告)日：2006-10-12

    申请号：US11388417

    申请日：2006-03-24

    申请人： Seong Lee ,  Soon-Kee Sung ,  Sung Kim ,  Jung Yi

    发明人： Seong Lee ,  Soon-Kee Sung ,  Sung Kim ,  Jung Yi

    IPC分类号： C07C237/02 ,  A61K8/46

    CPC分类号： C11C3/00 ,  C07C51/412 ,  C07C57/03 ,  C07C57/12

    摘要： Disclosed relates to a method for improving a conversion rate of oil soluble unsaturated lipids into water-soluble lipids and, more particularly, to a method for improving a conversion rate of oil soluble unsaturated lipids into water-soluble lipids that forms an unsaturated lipid-chloride derivative having an increased reaction activity by using a pyridine-thionyl chloride as a catalyst, not directly causing a reaction between the unsaturated lipids and amino acids, or that applies an emulsifier to cause a reaction between the unsaturated lipids and amino acids so as to minimize the size of lipid particles to a nano-size of less than 50 nm, thus improving the reaction conversion rate to more than 99%.

    摘要翻译： 本发明涉及提高油溶性不饱和脂质转化为水溶性脂质的转化率的方法，更具体地说，涉及提高油溶性不饱和脂质转化成形成不饱和脂质氯化物的水溶性脂质的转化率的方法 通过使用吡啶 - 亚硫酰氯作为催化剂具有增加的反应活性的衍生物，不直接引起不饱和脂和氨基酸之间的反应，或者使用乳化剂引起不饱和脂和氨基酸之间的反应，从而使 脂质颗粒的尺寸为纳米尺寸小于50nm，从而将反应转化率提高到99％以上。

    Cathode-to-cathode fuel cell stacks
    3.
    发明申请
    Cathode-to-cathode fuel cell stacks 有权
    阴极 - 阴极燃料电池堆

    公开(公告)号：US20060068235A1

    公开(公告)日：2006-03-30

    申请号：US10950714

    申请日：2004-09-27

    申请人： Gennady Resnick ,  Jung Yi ,  Tadahiko Taniguchi ,  Akira Maekawa

    发明人： Gennady Resnick ,  Jung Yi ,  Tadahiko Taniguchi ,  Akira Maekawa

    IPC分类号： H01M8/00

    CPC分类号： H01M8/04253 ,  H01M8/04007 ,  H01M8/249

    摘要： A plurality of fuel cell stacks (8, 8a, 9, 9a) have their cathode ends (11, 12) contiguous with either a common current collector (15a-15d) or respective current collectors (15a, 15b) which may be separated by electrical isolation (27a, 27b). The cathode-to-cathode relationship protects the cathode of each of the stacks from cold ambient environments, thereby permitting improved cold starts and mitigation of performance loss as a result of cold starts as well as freeze/thaw cycles. Heaters (30, 30a-30d) may be provided in current collectors, or in or between electrical isolation. Four stacks may share one current collector, or each may have its own current collector.

    摘要翻译： 多个燃料电池堆（8,8a，9,9a）的阴极端（11,12）与公共集电器（15a-15d）或相应的集电器（15a，15b）相连接 ），其可以通过电隔离（27a，27b）分离。 阴极到阴极的关系保护了每个堆叠的阴极与冷的环境环境，从而允许由于冷启动以及冷冻/融化循环而导致的冷启动和降低性能损失。 加热器（30,30a-30d）可以设置在集电器中，或在电隔离之间或之间。 四个堆叠可以共享一个集电器，或者每个可以具有其自己的集电器。

    Variants of glycoside hydrolases
    4.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US08383385B2

    公开(公告)日：2013-02-26

    申请号：US13009524

    申请日：2011-01-19

    申请人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C12S3/00 ,  C12S3/02 ,  C12P19/14 ,  C11D3/386

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Variants of glycoside hydrolases
    7.
    发明申请
    Variants of glycoside hydrolases 审中-公开
    糖苷水解酶的变体

    公开(公告)号：US20050048619A1

    公开(公告)日：2005-03-03

    申请号：US10926223

    申请日：2004-08-25

    申请人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C07H21/04 ,  C12N9/24 ,  C12P21/04

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176 ,  Y02E50/16

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Variants of glycoside hydrolases
    10.
    发明申请
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US20080167214A1

    公开(公告)日：2008-07-10

    申请号：US11891249

    申请日：2007-08-08

    申请人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C11D3/386 ,  C12N9/24 ,  C07H21/04 ,  C12N15/63 ,  C12N5/06 ,  C12P21/02 ,  B09C1/10

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。