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    Method of making biochips and the biochips resulting therefrom
    5.
    发明授权
    Method of making biochips and the biochips resulting therefrom 失效
    制作生物芯片的方法及由此产生的生物芯片

    公开(公告)号：US06174683B1

    公开(公告)日：2001-01-16

    申请号：US09299831

    申请日：1999-04-26

    申请人： Soonkap Hahn ,  Roberto Fagnani

    发明人： Soonkap Hahn ,  Roberto Fagnani

    IPC分类号： C12Q168

    CPC分类号： G01N33/54353 ,  G01N33/545

    摘要： Methods for preparing a biochip are provided herein wherein the biomolecular probe to be used with the biochip is alternatively bound to a hydrogel prepolymer prior to or simultaneously with polymerization of the prepolymer. In particularly preferred embodiments, a polyurethane-based hydrogel prepolymer is derivatized with an organic solvent soluble biomolecule, such as a peptide nucleic acid probe in aprotic, organic solvent. Following derivatization of the prepolymer, an aqueous solution, for example sodium bicarbonate, preferably buffered to a pH of about 7.2 to about 9.5, is added to the derivatized prepolymer solution to initiate polymerization of the hydrogel. Alternatively, a water soluble biomolecule, such as DNA or other oligonucleotide, is prepared in an aqueous solution and added to the polyurethane-based hydrogel prepolymer such that derivatization and polymerization occur, essentially, simultaneously. While the hydrogel is polymerizing, it is microspotted onto a solid substrate, preferably a silanated glass substrate, to which the hydrogel microdroplet becomes covalently bound. Most preferably the hydrogel microdroplets are at least about 30 &mgr;m thick, for example about 50 &mgr;m to about 100 &mgr;m thick. The resulting biochips are particularly useful for gene discovery, gene characterization, functional gene analysis and related studies.

    摘要翻译： 本文提供了制备生物芯片的方法，其中与生物芯片一起使用的生物分子探针在预聚物聚合之前或同时与水凝胶预聚物交替结合。 在特别优选的实施方案中，聚氨酯基水凝胶预聚物用有机溶剂可溶性生物分子衍生，例如非质子有机溶剂中的肽核酸探针。 在预聚物衍生化之后，向衍生化的预聚物溶液中加入优选缓冲至pH约7.2至约9.5的碳酸氢钠水溶液以引发水凝胶的聚合。 或者，在水溶液中制备水溶性生物分子，例如DNA或其它寡核苷酸，并加入到基于聚氨酯的水凝胶预聚物中，使得衍生化和聚合基本上同时发生。 当水凝胶聚合时，将其微滴在固体基质上，优选硅烷化的玻璃底物，水凝胶微滴变成共价键。 最优选地，水凝胶微滴至少约30μm厚，例如约50μm至约100μm厚。 所得到的生物芯片对于基因发现，基因表征，功能基因分析和相关研究特别有用。

    ISOLATING FETAL TROPHOBLASTS
    6.
    发明申请
    ISOLATING FETAL TROPHOBLASTS 审中-公开
    隔离FETL TROPHOBLASTS

    公开(公告)号：US20070224597A1

    公开(公告)日：2007-09-27

    申请号：US11277288

    申请日：2006-03-23

    申请人： Tony Pircher ,  Roberto Fagnani

    发明人： Tony Pircher ,  Roberto Fagnani

    IPC分类号： C12Q1/68 ,  C12N5/06

    CPC分类号： C12N5/0605 ,  C12N2509/00

    摘要： Methods for isolating and purifying fetal trophoblasts from a mucus sample obtained from the uterine cavity of a pregnant female. The mucus sample is transported from a clinical collection facility to a laboratory in a transportation medium so the cells remain viable. The mucus sample is then subjected to precise processing steps, including treatment with mucolytic agents or mucinases, sugar hydrolysis enzymes, nucleases, and proteases to provide fetal cells, the outer surfaces of which are so essentially completely devoid of attached mucosal biological material that they are then isolated in greater numbers than previously had been possible. The isolated cells are in appropriate condition to immediately be effectively subjected to FISH or to other molecular diagnostics.

    摘要翻译： 从怀孕女性的子宫腔获得的粘液样品中分离和纯化胎儿滋养层细胞的方法。 粘液样品从临床收集设备运输到运输介质中的实验室，以使细胞保持活力。 然后对粘液样品进行精确的加工步骤，包括用粘液分解剂或粘蛋白酶，糖水解酶，核酸酶和蛋白酶处理，以提供胎儿细胞，其外表基本上完全没有附着的粘膜生物材料，它们是 然后比以前更可能地分离出来。 分离的细胞处于适当条件下，以立即有效地进行FISH或其他分子诊断。