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公开(公告)号：US20050037343A1
公开(公告)日：2005-02-17
申请号：US10054728
申请日：2001-10-25
申请人： Roberto Fagnani , Soonkap Hahn , Xiaofan Dong , Tony Pircher , Sandra Matsumoto , Pavel Tsinberg
发明人： Roberto Fagnani , Soonkap Hahn , Xiaofan Dong , Tony Pircher , Sandra Matsumoto , Pavel Tsinberg
IPC分类号： C40B40/10 , C40B60/14 , G01N33/543 , G01N33/544 , G01N33/545 , C12Q1/68 , C12M1/34
CPC分类号： G01N33/54353 , B01J2219/00351 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14 , G01N33/5436 , G01N33/544 , G01N33/545
摘要： A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities are immobilized in these cells, which entities are effective to selectively hybridize to or sequester a target biomolecule. Different binding entities are immobilized in different cells in an array to create a biochip that can be used to assay for a number of target biomolecules.
摘要翻译： 生物芯片形成有多个光学透明的水凝胶细胞，其以阵列的形式附着在固体基质的顶表面上。 每个细胞由具有反应性异氰酸酯基团的聚乙二醇，聚丙二醇或其共聚物的水凝胶形成。 结合实体被固定在这些细胞中，哪些实体有效选择性地杂交或隔离靶生物分子。 将不同的结合实体固定在阵列中的不同细胞中以产生可用于测定多种靶生物分子的生物芯片。
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公开(公告)号：US07638464B2
公开(公告)日：2009-12-29
申请号：US10054728
申请日：2001-10-25
申请人： Roberto Fagnani , Soonkap Hahn , Xiaofan Dong , Tony Pircher , Sandra Matsumoto , Pavel Tsinberg
发明人： Roberto Fagnani , Soonkap Hahn , Xiaofan Dong , Tony Pircher , Sandra Matsumoto , Pavel Tsinberg
IPC分类号： C40B40/00
CPC分类号： G01N33/54353 , B01J2219/00351 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14 , G01N33/5436 , G01N33/544 , G01N33/545
摘要： A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities are immobilized in these cells, which entities are effective to selectively hybridize to or sequester a target biomolecule. Different binding entities are immobilized in different cells in an array to create a biochip that can be used to assay for a number of target biomolecules.
摘要翻译： 生物芯片形成有多个光学透明的水凝胶细胞，其以阵列的形式附着在固体基质的顶表面上。 每个细胞由具有反应性异氰酸酯基团的聚乙二醇，聚丙二醇或其共聚物的水凝胶形成。 结合实体被固定在这些细胞中，哪些实体有效选择性地杂交或隔离靶生物分子。 将不同的结合实体固定在阵列中的不同细胞中以产生可用于测定多种靶生物分子的生物芯片。
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3.发明申请METHODS AND GEL COMPOSITIONS FOR ENCAPSULATING LIVING CELLS AND ORGANIC MOLECULES 审中-公开封闭生殖细胞和有机分子的方法和凝胶组合物公开(公告)号：US20070141164A1
公开(公告)日：2007-06-21
申请号：US11670061
申请日：2007-02-01
申请人： Soonkap Hahn , Roberto Fagnani , Xiaofan Dong , Carl Edman , Pavel Tsinberg
发明人： Soonkap Hahn , Roberto Fagnani , Xiaofan Dong , Carl Edman , Pavel Tsinberg
IPC分类号： A61K9/50 , C08F283/04 , B01J13/02
CPC分类号： G01N33/5436 , A61K9/1641 , B01J19/0046 , B01J2219/00367 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743 , B82Y30/00 , C08G18/10 , C08G65/33348 , C08G65/3342 , C08G2210/00 , C08L2203/02 , C12N11/04 , C40B40/06 , C40B40/10 , C40B60/14 , C08G18/3876
摘要： A method for encapsulating biologics within a hydrogel by using an aqueous solution of an isocyanate-functional polyurethane prepolymer which is mixed with an amount of biologics and an aqueous solution containing a dithiol crosslinking agent under physiological pH conditions to create a hydrogel. An additional bidentate crosslinking agent is optionally included. The product of such method may be a bioreactor or an assay device having a plurality of different biologics encapsulated at predetermined locations in a substrate.
摘要翻译： 一种通过使用异氰酸酯官能的聚氨酯预聚物的水溶液将生物制剂包封在水凝胶内的方法，其在生理pH条件下与一定数量的生物制剂和含有二硫醇交联剂的水溶液混合以产生水凝胶。 任选地包括另外的二齿交联剂。 这种方法的产物可以是生物反应器或具有封装在基底中的预定位置处的多种不同生物制剂的测定装置。
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4.发明授权Methods and gel compositions for encapsulating living cells and organic molecules 有权用于包封活细胞和有机分子的方法和凝胶组合物公开(公告)号：US07172866B2
公开(公告)日：2007-02-06
申请号：US10398725
申请日：2002-04-02
申请人： Soonkap Hahn , Roberto Fagnani , Xiaofan Dong , Carl F. Edman , Pavel Tsinberg
发明人： Soonkap Hahn , Roberto Fagnani , Xiaofan Dong , Carl F. Edman , Pavel Tsinberg
IPC分类号： C12N5/00
CPC分类号： G01N33/5436 , A61K9/1641 , B01J19/0046 , B01J2219/00367 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743 , B82Y30/00 , C08G18/10 , C08G65/33348 , C08G65/3342 , C08G2210/00 , C08L2203/02 , C12N11/04 , C40B40/06 , C40B40/10 , C40B60/14 , C08G18/3876
摘要： A method for encapsulating biologics within a hydrogel by using an aqueous solution of an isocyanate-functional hydrogel prepolymer which is mixed with an amount of biologics and an aqueous solution containing a dithiol crosslinking agent under physiological pH conditions. An additional bidentate crosslinking agent may be included. The product of such method may be a bioreactor or an assay device having a plurality or different biologics encapsulated at predetermined locations in a substrate.
摘要翻译： 一种通过使用异氰酸酯官能的水凝胶预聚物的水溶液将生物制剂包封在水凝胶中的方法，该水溶液与一定数量的生物制剂和含有二硫醇交联剂的水溶液在生理pH条件下混合。 可以包括另外的二齿交联剂。 这种方法的产物可以是具有封装在基材中预定位置的多个或不同生物制剂的生物反应器或测定装置。
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公开(公告)号：US20100255479A1
公开(公告)日：2010-10-07
申请号：US12730738
申请日：2010-03-24
IPC分类号： G01N33/574 , G01N33/53 , C12M1/34 , C12Q1/68
CPC分类号： G01N33/54306 , G01N33/536 , G01N33/56966 , G01N33/57492
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
摘要翻译： 本发明提供了用于从样品，特别是生物样品中分离靶生物分子或细胞的装置。 具体地说，该装置包括载体混合物，其含有生物样品和特异性结合靶生物分子或靶细胞的第一结合实体; 以及涂覆有与第一结合实体直接或间接结合的第二结合实体的微通道。 还公开了在生物样品中捕获，检测和/或评估靶生物分子或靶细胞（例如癌细胞）的方法。
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公开(公告)号：US07238521B2
公开(公告)日：2007-07-03
申请号：US10722290
申请日：2003-11-24
CPC分类号： B01L3/50273 , B01F9/002 , B01F11/00 , B01F13/0059 , B01F13/02 , B01F2009/0092 , B01J2219/00484 , B01J2219/00493 , B01J2219/00533 , B01J2219/00659 , B01L3/502746 , B01L2300/0636 , B01L2300/0822 , B01L2400/0457 , B01L2400/086 , C12Q1/6837 , C40B60/14 , G01N35/00029 , G01N2035/00079 , G01N2035/00138
摘要： A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.
摘要翻译： 一种新颖的杂交装置，通过实现目标溶液的更大程度的内部混合来提高微阵列杂交反应的效率和一致性。 该设备提供了一种垫圈和盖型室，其中通过产生大量微泡来实现溶液混合。 限定腔室的一个或多个内壁包含延伸到腔室中并终止于尖锐边缘的气泡破裂元件。 它们通常位于矩形室的相对侧上并且指向与气泡运动相反的方向。 它们对较大气泡的干扰导致其分解成微泡，其由于外部搅拌而分开和不同的路径，从而提供改进的溶液混合，导致靶分子与结合到底物的探针分子的均匀分布。 杂交反应的灵敏度和一致性显着增加。
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公开(公告)号：US20120100538A1
公开(公告)日：2012-04-26
申请号：US13269532
申请日：2011-10-07
IPC分类号： G01N33/569 , G01N21/64 , C12M1/34 , C12Q1/68 , G01N33/574 , G01N33/82
CPC分类号： G01N33/57492 , C12Q1/6886 , C12Q2600/156 , G01N33/54306 , G01N33/56966 , G01N33/57415 , G01N33/57423 , G01N33/57434 , G01N33/57446 , G01N33/57496 , G01N2333/4742 , G01N2333/71
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
摘要翻译： 本发明提供了用于从样品，特别是生物样品中分离靶生物分子或细胞的装置。 具体地说，该装置包括载体混合物，其含有生物样品和特异性结合靶生物分子或靶细胞的第一结合实体; 以及涂覆有与第一结合实体直接或间接结合的第二结合实体的微通道。 还公开了在生物样品中捕获，检测和/或评估靶生物分子或靶细胞（例如癌细胞）的方法。
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公开(公告)号：US07217520B2
公开(公告)日：2007-05-15
申请号：US10823021
申请日：2004-04-12
CPC分类号： G01N21/03 , B01D61/18 , B01J2219/00286 , B01J2219/00317 , B01J2219/00385 , B01J2219/00423 , B01J2219/00576 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01L3/50255 , B01L2200/0689 , B01L2200/12 , B01L2300/0819 , B01L2400/049 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54366 , G01N33/54393 , G01N2021/0346
摘要： Microwell biochips (11) are formed from a thin flat plate (13) of polymeric material having a plurality of regularly spaced holes (15) that extend completely therethrough and create microwells. The lower end of each hole is closed by a microporous, hydrophobic, polymeric membrane (17) laminated to the undersurface of the plate which retains an aqueous test solution in the wells until a vacuum is applied to the undersurface thereof to effect draining of the solution and of any wash solution that might be subsequently added. A spot of polymerizing isocyanate-functional hydrogel is applied generally centrally to the porous membrane surface at the bottom of each well in a manner so as to cover only a minor portion of the surface and out of contact with the well sidewalls, thus leaving substantial surface area through which drainage can be readily effected. Biological capture agents are associated with the polymerizing hydrogel so as to become immobilized as a part thereof.
摘要翻译： 微孔生物芯片（11）由具有多个规则间隔开的孔（15）的聚合材料的薄平板（13）形成，所述孔完全延伸通过其形成微孔。 每个孔的下端由层压到板的下表面的微孔，疏水的聚合物膜（17）封闭，其将水性测试溶液保留在孔中，直到真空被施加到其下表面以实现溶液的排出 以及可能随后添加的任何洗涤溶液。 聚合异氰酸酯官能水凝胶的点通常以每个孔的底部中心地施加到多孔膜表面，以便仅覆盖表面的少部分并且不与阱侧壁接触，从而留下基本表面 可以容易地进行排水的区域。 生物捕获剂与聚合水凝胶相关联，以便作为其一部分被固定化。
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公开(公告)号：US20050112589A1
公开(公告)日：2005-05-26
申请号：US10722290
申请日：2003-11-24
IPC分类号： B01F9/00 , B01F11/00 , B01F13/00 , B01F13/02 , B01J19/00 , B01L3/00 , C12Q1/68 , C40B60/14 , G01N35/00 , G01N35/02 , C12M1/34
CPC分类号： B01L3/50273 , B01F9/002 , B01F11/00 , B01F13/0059 , B01F13/02 , B01F2009/0092 , B01J2219/00484 , B01J2219/00493 , B01J2219/00533 , B01J2219/00659 , B01L3/502746 , B01L2300/0636 , B01L2300/0822 , B01L2400/0457 , B01L2400/086 , C12Q1/6837 , C40B60/14 , G01N35/00029 , G01N2035/00079 , G01N2035/00138
摘要： A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.
摘要翻译： 一种新颖的杂交装置，通过实现目标溶液的更大程度的内部混合来提高微阵列杂交反应的效率和一致性。 该设备提供了一种垫圈和盖型室，其中通过产生大量微泡来实现溶液混合。 限定腔室的一个或多个内壁包含延伸到腔室中并终止于尖锐边缘的气泡破裂元件。 它们通常位于矩形室的相对侧上并且指向与气泡运动相反的方向。 它们对较大气泡的干扰导致其分解成微泡，其由于外部搅拌而分开和不同的路径，从而提供改进的溶液混合，导致靶分子与结合到底物的探针分子的均匀分布。 杂交反应的灵敏度和一致性显着增加。
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公开(公告)号：US09128082B2
公开(公告)日：2015-09-08
申请号：US12730738
申请日：2010-03-24
IPC分类号： G01N33/536 , G01N33/543 , G01N33/569
CPC分类号： G01N33/54306 , G01N33/536 , G01N33/56966 , G01N33/57492
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
摘要翻译： 本发明提供了用于从样品，特别是生物样品中分离靶生物分子或细胞的装置。 具体地说，该装置包括载体混合物，其含有生物样品和特异性结合靶生物分子或靶细胞的第一结合实体; 以及涂覆有与第一结合实体直接或间接结合的第二结合实体的微通道。 还公开了在生物样品中捕获，检测和/或评估靶生物分子或靶细胞（例如癌细胞）的方法。
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