公开(公告)号：US5647979A

公开(公告)日：1997-07-15

申请号：US666061

申请日：1996-06-14

申请人： Jia-Li Liao ,  Stellan Hjerten

发明人： Jia-Li Liao ,  Stellan Hjerten

IPC分类号： B01J20/285 ,  C08F2/44 ,  C08F18/08 ,  C08F20/06 ,  C08F20/12 ,  C08F20/32 ,  C08F20/34 ,  C08F20/56 ,  C08F20/58 ,  C08F26/02 ,  C08F28/02 ,  G01N30/56 ,  G01N30/88 ,  B01D15/08

CPC分类号： G01N30/482 ,  B01J2220/54 ,  G01N30/56

摘要： A separation medium for reversed-phase chromatography is prepared by polymerizing an aqueous vinyl monomer mixture to which a hydrophobic alkyl methacrylate with 3 or more carbon atoms in the alkyl group and a detergent have been added. The further inclusion of a vinyl monomer with a charged group in the reaction mixture produces a separation medium useful for reversed-phase electrochromatography. Performance of the polymerization in a capillary produces a continuous bed inside the capillary. An alternative for non-capillary separations, such as those conducted in larger diameter tubing, is the preparation of the medium in a vessel outside the tubing, followed by granulation of the polymer and placement of the granules in the tubing.

摘要翻译： 通过聚合乙烯基单体混合物制备反相色谱分离介质，其中烷基中具有3个或更多个碳原子的疏水性甲基丙烯酸烷基酯和已经加入了洗涤剂。 在反应混合物中进一步包含具有带电基团的乙烯基单体产生用于反相电色谱的分离介质。 在毛细管中聚合的性能在毛细管内产生连续的床。 用于非毛细管分离的替代方案，例如在较大直径的管中进行的那些，是在管外的容器中制备介质，然后将聚合物造粒并将颗粒放置在管道中。

公开(公告)号：US5814223A

公开(公告)日：1998-09-29

申请号：US961969

申请日：1997-10-31

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： B01J20/285 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  B01J20/291 ,  G01N30/02 ,  G01N30/88 ,  B01D15/08

CPC分类号： B01J20/291 ,  B01D15/3804 ,  B01J20/26 ,  B01J20/268 ,  B01J20/285 ,  B01J20/3057 ,  B01D15/3852 ,  B01J2220/54 ,  G01N30/02

摘要： Chromatographic media that demonstrate an artificially created recognition of any preselected molecular species with only an affinity-type adsorption are formed by polymerization from solutions of non-ionizable monomers with a quantity of the species contained in the solution, followed by removal of the species by conventional washing. The media are of particular utility and interest when used to achieve affinity binding of proteins. Uses of the media include chromatography and the general isolation and removal of the adsorbed species.

公开(公告)号：US5728296A

公开(公告)日：1998-03-17

申请号：US618879

申请日：1996-03-20

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： B01J20/285 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  B01J20/291 ,  G01N30/02 ,  G01N30/88 ,  B01D15/08

CPC分类号： B01J20/291 ,  B01D15/3804 ,  B01J20/26 ,  B01J20/268 ,  B01J20/285 ,  B01J20/3057 ,  B01D15/3852 ,  B01J2220/54 ,  G01N30/02

摘要： Chromatographic media that demonstrate an artificially created recognition of any preselected molecular species with only an affinity-type adsorption are formed by polymerization from solutions of non-ionizable monomers with a quantity of the species contained in the solution, followed by removal of the species by conventional washing. The media are of particular utility and interest when used to achieve affinity binding of proteins. Uses of the media include chromatography and the general isolation and removal of the adsorbed species.

摘要翻译： 证明人造产生的仅具有亲和型吸附的预选分子种类的识别色谱介质通过从非离子化单体与一定量溶液中所含物质的聚合反应形成，然后通过常规方法去除物种 洗涤。 当用于实现蛋白质的亲和力结合时，培养基具有特别的效用和兴趣。 介质的使用包括色谱法和吸附物质的一般分离和去除。

公开(公告)号：US5645717A

公开(公告)日：1997-07-08

申请号：US400419

申请日：1995-03-02

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  G01N30/52 ,  B01D15/08

CPC分类号： G01N30/482 ,  B01D15/34 ,  B01J20/26 ,  B01J20/281 ,  G01N30/48 ,  B01D15/327 ,  B01D15/361 ,  B01D15/3804 ,  B01J2220/54 ,  G01N2030/528

摘要： Macromolecular species in a liquid sample are chromatographically separated in a separation medium formed by polymerization of monomers in an aqueous solution with a sufficient amount of crosslinking agent to cause aggregation and precipitation of the polymer chains. The medium is either formed in the column in which chromatography is to take place as a continuous although channeled bed, or in a separate reaction vessel and then transferred to the column in comminuted form as a packed bed. Improvements in the performance of the bed are achieved by compression of the bed.

摘要翻译： 液体样品中的大分子物质在通过在水溶液中与单体在足够量的交联剂中聚合形成的分离介质中进行色谱分离，以引起聚合物链的聚集和沉淀。 培养基在柱中形成，其中色谱法将作为连续的通道床进行，或在单独的反应容器中形成，然后以粉碎形式作为填充床转移到柱中。 通过床的压缩来实现床的性能的改善。

公开(公告)号：US5464517A

公开(公告)日：1995-11-07

申请号：US380425

申请日：1995-01-30

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44747

摘要： Electrophoresis is performed in buffers which exhibit both substantial buffering capacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples:(1) buffering agents with a small number of charged groups per molecule;(2) carrier ampholytes fractionated to a narrow isoelectric point range;(3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and(4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values.These buffering agents are of particular interest in capillary electrophoresis.

摘要翻译： 电泳在缓冲液中进行，其表现出显着的缓冲能力和低电导率，允许在高场强下进行分离而不损失分辨率。 引用四类缓冲剂作为例子：（1）每分子具有少量带电基团的缓冲剂; （2）载体两性电解质分级至窄等电点范围; （3）低分子量缓冲两性电解质，其等电点与两性电解质的pK值之一接近; 和（4）高分子量缓冲两性电解质，其中酸性和碱性基团具有相同或非常接近的pK值。 这些缓冲剂在毛细管电泳中是特别感兴趣的。

公开(公告)号：US5938930A

公开(公告)日：1999-08-17

申请号：US939367

申请日：1997-09-29

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： G01N27/447 ,  G01N30/28 ,  G01N30/34 ,  G01N30/62 ,  B01D15/08

CPC分类号： G01N27/44747 ,  G01N30/34 ,  G01N2030/285 ,  G01N30/62

摘要： Peak resolution in reversed-phase electrochromatography is improved in either of two alternative ways. The first is by a stepwise increase in the concentration of acetonitrile or other equivalent buffer constituent or modifier after the sample has been loaded, and the second is the inclusion of a surfactant in the mobile phase.

公开(公告)号：US5916445A

公开(公告)日：1999-06-29

申请号：US960536

申请日：1997-10-13

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： B01J20/285 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  B01J20/291 ,  G01N30/02 ,  G01N30/88 ,  B01D15/08

CPC分类号： B01J20/291 ,  B01D15/3804 ,  B01J20/26 ,  B01J20/268 ,  B01J20/285 ,  B01J20/3057 ,  B01D15/3852 ,  B01J2220/54 ,  G01N30/02

摘要： Chromatographic media that demonstrate an artificially created recognition of any preselected molecular species with only can affinity-type adsorption are formed by polymerization from solutions of non-ionizable monomers with a quantity of the species contained in the solution, followed by removal of the species by conventional washing. The media are of particular utility and interest when used to achieve affinity binding of proteins. Uses of the media include chromatography and the general isolation and removal of the adsorbed species.

摘要翻译： 通过从非离子化单体的溶液与一定量的溶液中所含的物质的聚合反应，形成人造生物识别任何预选择的分子物种的色谱介质，仅通过亲和型吸附形成，然后通过常规方法除去物质 洗涤。 当用于实现蛋白质的亲和力结合时，培养基具有特别的效用和兴趣。 介质的使用包括色谱法和吸附物质的一般分离和去除。

公开(公告)号：US06423666B1

公开(公告)日：2002-07-23

申请号：US09412729

申请日：1999-10-05

申请人： Jia-Li Liao ,  Stellan Hjerten

发明人： Jia-Li Liao ,  Stellan Hjerten

IPC分类号： B01D1508

CPC分类号： G01N30/482 ,  B01J20/261 ,  B01J20/285 ,  B01J39/20 ,  B01J41/14 ,  B01J2220/54 ,  C08F2/32

摘要： Chromatographic beads with pores large enough to serve as through-channels for fast diffusion and a substantial absence of pores of smaller diameters that would cause band broadening due to slow diffusive transport, are formed by suspension polymerization of an aqueous solution of a monomer mixture that includes a total monomer (combination of polymer backbone monomer and crosslinking agent) concentration of 2% to 50% by weight relative to the aqueous solution and a crosslinking agent in an amount such that the mole ratio of crosslinking agent to total monomer is from about 0.1 to about 0.7. The suspension polymerization process involves forming a suspension of aqueous droplets of the monomer mixture in an organic phase.

摘要翻译： 通过悬浮聚合单体混合物的水溶液形成具有足够大孔的色谱珠，其通过悬浮聚合单体混合物的水溶液而形成，该孔具有足够大的通孔用于快速扩散，并且基本上不存在由于缓慢的扩散输送而导致带宽扩大的较小直径的孔 相对于水溶液和交联剂的总单体（聚合物主链单体和交联剂的组合）浓度为2重量％至50重量％，其量使得交联剂与总单体的摩尔比为约0.1至 约0.7。 悬浮聚合方法包括在有机相中形成单体混合物的水性液滴的悬浮液。

公开(公告)号：US6054053A

公开(公告)日：2000-04-25

申请号：US313943

申请日：1999-05-18

申请人： Stellan Hjerten ,  Jia-Li Liao

发明人： Stellan Hjerten ,  Jia-Li Liao

IPC分类号： G01N27/447 ,  G01N30/28 ,  G01N30/34 ,  G01N30/62 ,  B01D15/08

CPC分类号： G01N27/44747 ,  G01N30/34 ,  G01N2030/285 ,  G01N30/62

摘要： Peak resolution in reversed-phase electrochromatography is improved in either of two alternative ways. The first is by a stepwise increase in the concentration of acetonitrile or other equivalent buffer constituent or modifier after the sample has been loaded, and the second is the inclusion of a surfactant in the mobile phase.

摘要翻译： 反相电色谱中的峰分辨率以两种替代方式中的任一种改进。 第一种是在样品加载后乙腈或其他等效的缓冲成分或改性剂的浓度逐步增加，第二种是在流动相中包含表面活性剂。

公开(公告)号：US5935429A

公开(公告)日：1999-08-10

申请号：US964768

申请日：1997-11-05

申请人： Jia-Li Liao ,  Stellan Hjerten

发明人： Jia-Li Liao ,  Stellan Hjerten

IPC分类号： G01N33/50 ,  B01D15/08 ,  B01J20/281 ,  B01J20/285 ,  C08F2/10 ,  C08F18/00 ,  C08F18/08 ,  C08F20/00 ,  C08F20/06 ,  C08F20/10 ,  C08F20/56 ,  C08F20/58 ,  G01N30/56 ,  G01N30/88 ,  G01N33/68

CPC分类号： G01N30/482 ,  B01D15/1807 ,  B01D15/361 ,  G01N30/56 ,  B01J2220/54

摘要： A chromatography column with a continuous solid bed spanning the cross section of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solution. The monomers include a monofunctional monomer such as a vinyl, allyl, acrylic, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), the total monomer concentration being in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammonium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

摘要翻译： 通过在水溶液中聚合单体和硫酸铵的混合物，制备了具有横跨柱截面的连续固体床的层析柱，该床含有足够大的流体动力学流动的通道。 单体包括单官能单体如乙烯基，烯丙基，丙烯酸或甲基丙烯酸化合物和多官能单体（即交联剂），总单体浓度在10-20重量％的范围内，摩尔比 的交联剂与总单体的比例在0.3至0.4范围内，而硫酸铵的浓度范围为0.4M至0.8M。赋予专门分离能力，特别是阴离子和阳离子交换的官能团可以包括在 单体混合物。