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公开(公告)号:US20080038163A1
公开(公告)日:2008-02-14
申请号:US11757286
申请日:2007-06-01
申请人: Steven Boege , John Sheridan , Ali Aslam , Dennis Lehto , Julio Focaracci , Alexander Dromaretsky , Thomas Au , Mark Oldham , Eric Henderson , Vinod Mirchandani , Eric Nordman , Marc Haberstroh , Johannes Sluis
发明人: Steven Boege , John Sheridan , Ali Aslam , Dennis Lehto , Julio Focaracci , Alexander Dromaretsky , Thomas Au , Mark Oldham , Eric Henderson , Vinod Mirchandani , Eric Nordman , Marc Haberstroh , Johannes Sluis
CPC分类号: B01L7/52 , B01L2300/0636 , B01L2300/0829 , B01L2300/0877 , B01L2300/1822 , B01L2300/1844 , B01L2300/185
摘要: A device for performing biological analysis may include at least one reaction chamber configured to receive at least one sample for biological analysis and a thermal system configured to modulate a temperature of the at least one reaction chamber to cycle a temperature of the at least one biological sample. The thermal system may include a cooling system configured to cool the at least one reaction chamber. The cooling system may include a cooling fluid source positioned distally from the at least one reaction chamber, the cooling fluid source being in flow communication with at least one conduit configured to flow cooling fluid from the cooling fluid source to at least one location in thermal communication with the at least one reaction chamber.
摘要翻译: 用于进行生物分析的装置可以包括至少一个反应室,其配置成容纳至少一个用于生物分析的样品,以及被配置为调节至少一个反应室的温度以循环至少一个生物样品的温度的热系统 。 热系统可以包括被配置为冷却至少一个反应室的冷却系统。 所述冷却系统可以包括从所述至少一个反应室向远侧定位的冷却流体源,所述冷却流体源与至少一个管道流动连通,所述至少一个导管构造成将来自所述冷却流体源的冷却流体流动到热通信中的至少一个位置 与至少一个反应室。
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公开(公告)号:US20070014695A1
公开(公告)日:2007-01-18
申请号:US11380327
申请日:2006-04-26
申请人: Min Yue , David Liu , Yuh-Min Chiang , Joon Mo Yang , Dennis Lehto , Charles Vann , Nigel Beard , Ian Harding , John Van Camp , Alexander Dromaretsky , Sergey Ermakov , Mark Oldham , Joy Roy , Maryam Shariati , Umberto Ulmanella
发明人: Min Yue , David Liu , Yuh-Min Chiang , Joon Mo Yang , Dennis Lehto , Charles Vann , Nigel Beard , Ian Harding , John Van Camp , Alexander Dromaretsky , Sergey Ermakov , Mark Oldham , Joy Roy , Maryam Shariati , Umberto Ulmanella
IPC分类号: B01L3/00
CPC分类号: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
摘要: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
摘要翻译: 用于多个分析物检测的系统和方法包括用于分配包括基底的生物样品的系统,其中所述基底包括多个样品室,用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测相容和测定相容的材料构成。
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公开(公告)号:US08597590B2
公开(公告)日:2013-12-03
申请号:US12789363
申请日:2010-05-27
申请人: Min Yue , David M. Liu , Joy Roy , Yuh-Min Chiang , Joon Mo Yang , Dennis Lehto , Charles S. Vann , Nigel P. Beard , Ian A. Harding , John R. Van Camp , Alexander Dromaretsky , Sergey V. Ermakov , Mark F. Oldham , Maryam Shariati , Umberto Ulmanella
发明人: Min Yue , David M. Liu , Joy Roy , Yuh-Min Chiang , Joon Mo Yang , Dennis Lehto , Charles S. Vann , Nigel P. Beard , Ian A. Harding , John R. Van Camp , Alexander Dromaretsky , Sergey V. Ermakov , Mark F. Oldham , Maryam Shariati , Umberto Ulmanella
IPC分类号: G01N21/75
CPC分类号: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
摘要: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
摘要翻译: 用于多个分析物检测的系统和方法包括用于分配包括基底的生物样品的系统,其中所述基底包括多个样品室,用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测相容和测定相容的材料构成。
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公开(公告)号:US20110020179A1
公开(公告)日:2011-01-27
申请号:US12789363
申请日:2010-05-27
申请人: Min Yue , David M. Liu , Yuh-Min Chiang , Joon-Mo Yang , Dennis Lehto , Charles S. Vann , Nigel P. Beard , Ian A. Harding , John Van Camp , Alexander Dromaretsky , Sergey V. Ermakov , Mark F. Oldham , Joy Roy , Maryam Shariati , Umberto Ulmanella
发明人: Min Yue , David M. Liu , Yuh-Min Chiang , Joon-Mo Yang , Dennis Lehto , Charles S. Vann , Nigel P. Beard , Ian A. Harding , John Van Camp , Alexander Dromaretsky , Sergey V. Ermakov , Mark F. Oldham , Joy Roy , Maryam Shariati , Umberto Ulmanella
IPC分类号: G01N33/00
CPC分类号: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
摘要: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
摘要翻译: 用于多个分析物检测的系统和方法包括用于分配包括基底的生物样品的系统,其中所述基底包括多个样品室,用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测相容和测定相容的材料构成。
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公开(公告)号:US20120057366A1
公开(公告)日:2012-03-08
申请号:US13267787
申请日:2011-10-06
CPC分类号: G02B6/351 , G01S7/4818 , G01S7/495 , G02B6/0001 , G02B6/3512 , G02B6/3528 , G02B6/3558 , G02B6/3582
摘要: An optical fiber switch (16) for alternatively redirecting an input beam (14) comprises a redirector (18) and a redirector mover (20). The redirector (18) redirects the input beam (14) so that a redirected beam (46) alternatively launches from the redirector (18) (i) along a first redirected axis (354) that is spaced apart from a directed axis (344A) when the redirector (18) is positioned at a first position (348), and (ii) along a second redirected axis (356) that is spaced apart from the directed axis (344A) when the redirector (18) is positioned at a second position (350) that is different from the first position (348). The redirector mover (20) moves the redirector (18) about a movement axis (366) between the first position (348) and the second position (350).
摘要翻译: 用于交替重定向输入光束(14)的光纤开关(16)包括重定向器(18)和重定向器移动器(20)。 重定向器(18)重定向输入光束(14),使得重定向光束(46)可以沿着与指向轴线(344A)间隔开的第一重定向轴线(354)从转向器(18)(i)发射, 当重定向器(18)位于第二位置(348)时,当转向器(18)位于第二位置(348)时,以及(ii)沿着与定向轴线(344A)间隔开的第二重定向轴线(356) 位置(350)与第一位置(348)不同。 重定向器移动器(20)围绕位于第一位置(348)和第二位置(350)之间的移动轴线(366)移动重定向器(18)。
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公开(公告)号:US20080124722A1
公开(公告)日:2008-05-29
申请号:US11767323
申请日:2007-06-22
CPC分类号: B01L7/52 , B01L2300/0636 , B01L2300/0829 , B01L2300/0877 , B01L2300/1822 , B01L2300/1844 , B01L2300/185
摘要: A device for amplifying a nucleic acid sample may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may include at least one heat pipe and a heat sink and the at least one heat pipe may include a first portion disposed proximate to the sample holder and a second portion disposed proximate to the heat sink.
摘要翻译: 用于扩增核酸样品的装置可以包括配置成接收核酸样品的样品保持器,被配置为升高样品温度的加热系统,被配置为降低样品温度的冷却系统,和被配置为 可操作地控制加热系统和冷却系统以使设备循环所需的时间 - 温度分布。 冷却系统可以包括至少一个热管和散热器,并且所述至少一个热管可以包括靠近样品保持器设置的第一部分和靠近散热器设置的第二部分。
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公开(公告)号:US08335413B2
公开(公告)日:2012-12-18
申请号:US13267787
申请日:2011-10-06
CPC分类号: G02B6/351 , G01S7/4818 , G01S7/495 , G02B6/0001 , G02B6/3512 , G02B6/3528 , G02B6/3558 , G02B6/3582
摘要: An optical fiber switch (16) for alternatively redirecting an input beam (14) comprises a redirector (18) and a redirector mover (20). The redirector (18) redirects the input beam (14) so that a redirected beam (46) alternatively launches from the redirector (18) (i) along a first redirected axis (354) that is spaced apart from a directed axis (344A) when the redirector (18) is positioned at a first position (348), and (ii) along a second redirected axis (356) that is spaced apart from the directed axis (344A) when the redirector (18) is positioned at a second position (350) that is different from the first position (348). The redirector mover (20) moves the redirector (18) about a movement axis (366) between the first position (348) and the second position (350).
摘要翻译: 用于交替重定向输入光束(14)的光纤开关(16)包括重定向器(18)和重定向器移动器(20)。 重定向器(18)重定向输入光束(14),使得重定向光束(46)可以沿着与指向轴线(344A)间隔开的第一重定向轴线(354)从转向器(18)(i)发射, 当重定向器(18)位于第二位置(348)时,当转向器(18)位于第二位置(348)时,以及(ii)沿着与定向轴线(344A)间隔开的第二重定向轴线(356) 位置(350)与第一位置(348)不同。 重定向器移动器(20)围绕位于第一位置(348)和第二位置(350)之间的移动轴线(366)移动重定向器(18)。
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公开(公告)号:US06676266B2
公开(公告)日:2004-01-13
申请号:US10001461
申请日:2001-10-29
IPC分类号: G02B7182
CPC分类号: G02B27/145 , G02B21/082 , G02B21/16
摘要: An optical pathway selector is provided having a first direction selector, a second direction selector, and an actuator. The first direction selector is comprised of a substantially planar member having alternating reflecting and transparent portions. The first direction selector is disposed for rotation on an axle with the substantially planar member positioned askew to a first optical axis. The second direction selector has a configuration similar to that of the first direction selector and is disposed along the axle substantially parallel to the first direction selector. Additional direction selectors can be employed in a similar manner. A motor is connected to the axle for rotating the direction selectors. Alternatively, a motor moves the direction selector along a linear pathway in and out of the pathway of different light beams. The optical pathway selector is used to reflect a beam of light from the first optical axis to alternative optical axes or vice-versa. A retroreflector may be utilized in conjunction with the optical pathway selector to eliminate error due to non-planar surfaces or motion. The optical pathway selector may be used in epifluorescence imaging systems.
摘要翻译: 提供了具有第一方向选择器,第二方向选择器和致动器的光学路径选择器。 第一方向选择器由具有交替的反射和透明部分的基本平坦的部件组成。 第一方向选择器设置成在轴上旋转,其中基本上平面的构件朝向第一光轴倾斜。 第二方向选择器具有与第一方向选择器类似的构造,并且沿着大致平行于第一方向选择器的轴布置。 附加方向选择器可以以类似的方式使用。 电动机连接到车轴以旋转方向选择器。 或者,马达沿着沿着不同光束的通路的线性通道移动方向选择器。 光路径选择器用于将来自第一光轴的光束反射到备选的光轴,反之亦然。 后向反射器可以与光学路径选择器一起使用以消除由于非平面表面或运动引起的误差。 光路径选择器可用于落射荧光成像系统。
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公开(公告)号:US06555802B2
公开(公告)日:2003-04-29
申请号:US09755725
申请日:2001-01-05
IPC分类号: G02B2600
CPC分类号: G02B21/16 , G02B21/002 , G02B21/02
摘要: A scanning microscope. An objective lens receives light emitted from a sample in object space and propagates it to image space thereof. A collection lens receives light from the objective lens and propagates it to a focal point in image space of the collection lens. A motor has an axis of rotation that is offset from and extends in substantially the same direction as the optical axis. The motor rotates the objective lens about the axis of rotation to scan across a sample in object space of said objective lens. The sample is mounted on a stage. After each rotation of the objective lens, the stage is advanced in a radial direction with respect to the axis of rotation so that each subsequent scan covers a new part of the sample. For fluorescence microscopy, a laser light source is provided. A wavelength-selective beamsplitter directs the laser light toward the objective lens, while allowing fluorescence or reflected light emitted from the sample to pass through to the collection lens. A photo detector is placed at the image space focal point of the collection lens so as to convert the intensity of the light emitted from the sample at the focal point of the objective lens in object space to an electrical signal. To achieve high resolution, a light-path-altering optical element is inserted between the light source and the objective lens so as to translate the light centered on the axis of rotation to light centered on the optical axis of the objective lens, thereby filling the entrance pupil of the objective lens.
摘要翻译: 扫描显微镜。 物镜接收从物体空间中的样品发射的光并将其传播到其图像空间。 收集透镜从物镜接收光并将其传播到收集透镜的图像空间中的焦点。 电动机具有偏离轴线并且沿与光轴基本相同的方向延伸。 电动机围绕旋转轴旋转物镜以在物镜的物体空间中扫描样品。 样品安装在舞台上。 在物镜每转动一次之后,平台相对于旋转轴线在径向方向上前进,使得每个随后的扫描覆盖样品的新部分。 对于荧光显微镜,提供激光光源。 波长选择性分束器将激光指向物镜,同时允许从样品发射的荧光或反射光通过到收集透镜。 将光检测器放置在收集透镜的图像空间焦点处,以将从物体的物镜焦点处的样品发射的光的强度转换为电信号。 为了实现高分辨率,将光路改变的光学元件插入在光源和物镜之间,以将以旋转轴为中心的光转移到以物镜的光轴为中心的光,从而填充 入射光瞳的物镜。
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公开(公告)号:US20110256616A1
公开(公告)日:2011-10-20
申请号:US12985588
申请日:2011-01-06
IPC分类号: C12M1/40
CPC分类号: B01L7/52 , B01L2300/0636 , B01L2300/0829 , B01L2300/0877 , B01L2300/1822 , B01L2300/1844 , B01L2300/185
摘要: A device for amplifying a nucleic acid sample may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may include at least one heat pipe and a heat sink and the at least one heat pipe may include a first portion disposed proximate to the sample holder and a second portion disposed proximate to the heat sink.
摘要翻译: 用于扩增核酸样品的装置可以包括配置成接收核酸样品的样品保持器,被配置为升高样品温度的加热系统,被配置为降低样品温度的冷却系统,和被配置为 可操作地控制加热系统和冷却系统以使设备循环所需的时间 - 温度分布。 冷却系统可以包括至少一个热管和散热器,并且所述至少一个热管可以包括靠近样品保持器设置的第一部分和靠近散热器设置的第二部分。
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