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    High throughput screening of potential displacer molecules
    1.
    发明申请
    High throughput screening of potential displacer molecules 审中-公开
    潜在置换剂分子的高通量筛选

    公开(公告)号：US20050176937A1

    公开(公告)日：2005-08-11

    申请号：US11103293

    申请日：2005-04-11

    申请人： Steven Cramer ,  Kaushal Rege ,  Jonathan Dordick ,  Nihal Tugcu

    发明人： Steven Cramer ,  Kaushal Rege ,  Jonathan Dordick ,  Nihal Tugcu

    IPC分类号： B01D15/42 ,  C07K1/22 ,  C07H21/04 ,  C07K1/10

    CPC分类号： B01D15/422 ,  C07K1/22

    摘要： A bioproduct may be selectively separated from one or more impurities by means of a displacement chromatography system that includes a solvent, a chromatographic resin and a chemically selective displacer. The method includes: dissolving the bioproduct and the one or more impurities in a solvent; loading the bioproduct and the one or more impurities, in the solvent, on a chromatographic resin; displacing the bioproduct from the chromatographic resin with chemically selective displacer; and retaining the one or more impurities on the chromatographic resin. For this method, the bioproduct and the impurities have similar binding affinity for the chromatographic resin in the absence of the displacer.

    摘要翻译： 生物产物可以通过置换色谱系统选择性地与一种或多种杂质分离，所述置换色谱系统包括溶剂，色谱树脂和化学选择性置换器。 该方法包括：将生物产物和一种或多种杂质溶解在溶剂中; 在溶剂中将生物产物和一种或多种杂质装载在色谱树脂上; 用化学选择性置换器置换色谱树脂的生物产物; 并将一种或多种杂质保留在色谱树脂上。 对于该方法，生物产物和杂质在没有置换剂的情况下对色谱树脂具有相似的结合亲和力。

    High throughtput screening of potential displacer molecules
    2.
    发明授权
    High throughtput screening of potential displacer molecules 有权
    潜在置换剂分子的高通量筛选

    公开(公告)号：US06881540B2

    公开(公告)日：2005-04-19

    申请号：US09791317

    申请日：2001-02-23

    申请人： Steven Cramer ,  Kaushal Rege ,  Jonathan Dordick

    发明人： Steven Cramer ,  Kaushal Rege ,  Jonathan Dordick

    IPC分类号： B01D15/42 ,  C07K1/22 ,  C12Q1/68

    CPC分类号： C07K1/22 ,  B01D15/422

    摘要： An effective technique for the high throughput screening of displacers is described. In this technique, potential displacers are employed to displace a biomolecule (e.g., protein) adsorbed on a chromatographic resin in small-scale batch displacement experiments. The amount of protein displaced from a specific resin by a defined concentration of displacer is determined by monitoring the supermatant for the protein. By evaluating the displaced protein rather than the displacer itself, this technique enables a single detection technique (e.g., absorbance, fluorescence, etc.) to be employed for all batch displacement experiments. By monitoring the amount of protein displaced, the effacy of a large number of potential displacers can be rapidly evaluated. The entire experimental procedure can be carried out rapidly and is thus amenable to high throughput parallel screening of molecules possessing a large range of affinities and physico-chemical properties. The error of the technique is within 5% of protein displaced, thus making it a very reliable technique. The technique can be extended to different stationary phase materials, biomolecules, and modes of interaction.

    摘要翻译： 描述了用于置换器的高通量筛选的有效技术。 在该技术中，使用潜在的置换剂来置换小规模批量置换实验中吸附在色谱树脂上的生物分子（例如蛋白质）。 通过监测蛋白质的超级蛋白质来确定由特定树脂从限定浓度的置换剂中排出的蛋白质的量。 通过评估置换的蛋白质而不是置换剂本身，该技术使得能够将单个检测技术（例如，吸光度，荧光等）用于所有的批量置换实验。 通过监测蛋白质置换量，可以快速评估大量潜在置换剂的效率。 整个实验程序可以快速进行，因此适合高通量平行筛选具有大范围亲和力和物理化学性质的分子。 该技术的误差在蛋白质置换的5％以内，因此使其成为非常可靠的技术。 该技术可以扩展到不同的固定相材料，生物分子和相互作用模式。

    Enzyme immobilization for electroosmotic flow
    4.
    发明申请
    Enzyme immobilization for electroosmotic flow 审中-公开
    酶固定用于电渗流

    公开(公告)号：US20070108057A1

    公开(公告)日：2007-05-17

    申请号：US11651269

    申请日：2007-01-09

    申请人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

    发明人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

    IPC分类号： G01N27/00

    CPC分类号： C07K17/14

    摘要： Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

    摘要翻译： 本文公开了将生物催化剂固定在微流体生物芯片上以在存在电渗流的情况下进行反应的方法和装置。 生物芯片包括在其微流体流动表面上的聚合物，其中聚合物包括选自相同极性的离子基团的第一取代基或其前体，作为疏水基团的第二取代基和包含固定的生物催化剂或前体的第三取代基 其中。 生物芯片可用于在电渗流的存在下进行多次连续的生物催化反应。

    Patterning of centrosomes and centrosome fragments as templates for directed growth of microtubules
    5.
    发明申请
    Patterning of centrosomes and centrosome fragments as templates for directed growth of microtubules 审中-公开
    中心体和中心体片段的图案作为微管定向生长的模板

    公开(公告)号：US20060263832A1

    公开(公告)日：2006-11-23

    申请号：US11435156

    申请日：2006-05-16

    申请人： Wen Shang ,  Jonathan Dordick ,  Robert Palazzo ,  Richard Siegel

    发明人： Wen Shang ,  Jonathan Dordick ,  Robert Palazzo ,  Richard Siegel

    IPC分类号： G01N33/53 ,  C12N9/00 ,  C12M1/34

    CPC分类号： G01N33/543 ,  G01N33/68

    摘要： The present invention relates to a new process to direct the growth and direction of polymerization of microtubules using patterned centrosomes or centrosome fragments on a surface. Incorporation a flow force to direct the position and the growth of microtubules, results in a regular network of microtubules. The invention therefore provides a new route to develop both sensing and non-sensing functional microtubule-based nanodevices such as those for nanoscale separation or purification.

    摘要翻译： 本发明涉及使用图案化的中心体或中心体片段在表面上引导微管的生长和聚合方向的新方法。 引入流动力来引导微管的位置和生长，导致微管的规则网络。 因此，本发明提供了开发基于感测和非感测功能的基于微管的纳米器件的新途径，例如用于纳米尺度分离或纯化的纳米器件。

    ENZYME IMMOBILIZATION FOR ELECTROOSMOTIC FLOW
    7.
    发明申请
    ENZYME IMMOBILIZATION FOR ELECTROOSMOTIC FLOW 有权
    电动流动的酶固定

    公开(公告)号：US20070000781A1

    公开(公告)日：2007-01-04

    申请号：US10351976

    申请日：2003-01-24

    申请人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

    发明人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

    IPC分类号： C07K1/26 ,  G01N27/00

    CPC分类号： C07K17/14

    摘要： Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst- or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

    摘要翻译： 本文公开了将生物催化剂固定在微流体生物芯片上以在存在电渗流的情况下进行反应的方法和装置。 生物芯片包括其微流体流动表面上的聚合物，其中聚合物包括选自相同极性的离子基团的第一取代基或其前体，作为疏水基团的第二取代基，和包含固定的生物催化剂或前体的第三取代基 其中。 生物芯片可用于在电渗流的存在下进行多次连续的生物催化反应。