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    METHOD FOR INTRODUCING NUCLEIC ACIDS INTO FUNGAL CELLS
    2.
    发明申请
    METHOD FOR INTRODUCING NUCLEIC ACIDS INTO FUNGAL CELLS 有权
    将核酸引入真核细胞的方法

    公开(公告)号：US20100304468A1

    公开(公告)日：2010-12-02

    申请号：US12600452

    申请日：2008-05-21

    申请人： Steven Kim ,  Andrei Miasnikov

    发明人： Steven Kim ,  Andrei Miasnikov

    IPC分类号： C12N1/15

    CPC分类号： C12N15/87

    摘要： The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.

    摘要翻译： 本文公开的方法通常涉及将诸如生物分子（例如核酸）的分子引入丝状真菌中。 更具体地，本文公开的方法涉及将一种或多种核酸引入丝状真菌中。

    PROTEIN
    6.
    发明申请
    PROTEIN 有权
    蛋白

    公开(公告)号：US20120190072A1

    公开(公告)日：2012-07-26

    申请号：US13329531

    申请日：2011-12-19

    申请人： Andrei Miasnikov ,  Richard R. Bott ,  Jens Frisbaek Sørensen

    发明人： Andrei Miasnikov ,  Richard R. Bott ,  Jens Frisbaek Sørensen

    IPC分类号： C12N9/18 ,  C12P19/00 ,  C12P9/00 ,  A21D10/00 ,  A21D8/04 ,  A23L1/32 ,  A23L1/00 ,  A21D13/00 ,  C07H21/04 ,  C12S99/00

    CPC分类号： C12N9/20 ,  A21D8/042 ,  C12N9/18 ,  C12Y301/01003 ,  C12Y301/01004 ,  C12Y301/01026 ,  C12Y301/01032

    摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

    摘要翻译： 本发明涉及一种制备变体脂肪分解酶的方法，包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包含至少一个在 编码的氨基酸序列以a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点; b）在更亲水的酶的活性位点的远端的外部环的表面位置和位置处引入至少一个氨基酸; 或c）在本文公开的一个或多个位置的取代或插入或在本文公开的一个或多个位置处的缺失。 本发明还涉及通过该方法产生的多肽和新的核酸。