公开(公告)号：US08574635B2

公开(公告)日：2013-11-05

申请号：US12932018

申请日：2011-02-16

申请人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z. H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

发明人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z. H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

IPC分类号： A01N65/00

CPC分类号： A61K31/7048 ,  A61K31/05 ,  A61K31/155 ,  A61K31/175 ,  A61K31/351 ,  A61K31/366 ,  A61K31/40 ,  A61K31/404 ,  A61K31/427 ,  A61K31/435 ,  A61K31/4439 ,  A61K31/445 ,  A61K31/70 ,  A61K31/7036 ,  A61K36/00 ,  A61K38/28 ,  A61K45/06

摘要： The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening/diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

公开(公告)号：US20110142973A1

公开(公告)日：2011-06-16

申请号：US12932018

申请日：2011-02-16

申请人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z.H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

发明人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z.H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

IPC分类号： A61K36/63 ,  A61P3/00

CPC分类号： A61K31/7048 ,  A61K31/05 ,  A61K31/155 ,  A61K31/175 ,  A61K31/351 ,  A61K31/366 ,  A61K31/40 ,  A61K31/404 ,  A61K31/427 ,  A61K31/435 ,  A61K31/4439 ,  A61K31/445 ,  A61K31/70 ,  A61K31/7036 ,  A61K36/00 ,  A61K38/28 ,  A61K45/06

摘要： The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening/diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

摘要翻译： 本发明涉及通过给予橄榄苷，类似物或衍生物来治疗患有或倾向于发生肥胖或肥胖相关病症或抑制HIV感染性的受试者中的肥胖或肥胖相关疾病的方法和药物组合物 或橄榄苦蛋白的主要代谢物，包括橄榄苷苷苷元，羟基酪醇和烯诺酸或其类似物或衍生物，环烯醚萜苷，或其类似物或衍生物，或上述物质的任何组合，包括橄榄果提取物。 本发明还涉及通过测量选自PPARγ2，LPL和αP2基因的脂肪形成基因和基因产物或其它脂肪生成，脂质生成的脂肪形成基因的表达谱来筛选/诊断具有或倾向于具有肥胖症或相关病症的受试者的方法， 或脂肪分解基因和基因产物。 本发明进一步提供了新的橄榄苦苷类似物的筛选。

公开(公告)号：US20090061031A1

公开(公告)日：2009-03-05

申请号：US11827135

申请日：2007-07-09

申请人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z.H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

发明人： Sylvia Lee-Huang ,  Paul L. Huang ,  Philip Lin Huang ,  Dawei Zhang ,  John Z.H. Zhang ,  Young Tae Chang ,  Jae Wook Lee ,  Ju Bao ,  Yongtao Sun

IPC分类号： A61K36/00 ,  A61K31/351 ,  A61K31/70 ,  A61K38/28 ,  A61K31/4439 ,  A61K31/435 ,  A61K31/366 ,  A61K31/7036 ,  A61P3/00 ,  A61P31/12 ,  A61K31/175 ,  A61K31/445 ,  A61K31/404 ,  A61K31/40 ,  A61K31/427 ,  A61K31/155 ,  A61K31/05

CPC分类号： A61K31/7048 ,  A61K31/05 ,  A61K31/155 ,  A61K31/175 ,  A61K31/351 ,  A61K31/366 ,  A61K31/40 ,  A61K31/404 ,  A61K31/427 ,  A61K31/435 ,  A61K31/4439 ,  A61K31/445 ,  A61K31/70 ,  A61K31/7036 ,  A61K36/00 ,  A61K38/28 ,  A61K45/06

摘要： The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening/diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

摘要翻译： 本发明涉及通过给予橄榄苷，类似物或衍生物来治疗患有或倾向于发生肥胖或肥胖相关病症或抑制HIV感染性的受试者中的肥胖或肥胖相关疾病的方法和药物组合物 或橄榄苦蛋白的主要代谢物，包括橄榄苷苷苷元，羟基酪醇和烯诺酸或其类似物或衍生物，环烯醚萜苷，或其类似物或衍生物，或上述物质的任何组合，包括橄榄果提取物。 本发明还涉及通过测量选自PPARgamma2，LPL和αP2基因的脂肪形成基因和基因产物或其它脂肪形成，脂质生成，或其它成分的脂肪形成基因的表达谱来筛选/诊断具有或倾向于具有肥胖症或相关病症的受试者的方法。 或脂肪分解基因和基因产物。 本发明进一步提供了新的橄榄苦苷类似物的筛选。

公开(公告)号：US5484889A

公开(公告)日：1996-01-16

申请号：US277283

申请日：1994-07-21

申请人： Sylvia Lee-Huang ,  Philip L. Huang ,  Peter L. Nara ,  Hao-Chia Chen ,  Hsiang-fu Kung ,  Peter Huang ,  Henry I. Huang ,  Paul L. Huang

发明人： Sylvia Lee-Huang ,  Philip L. Huang ,  Peter L. Nara ,  Hao-Chia Chen ,  Hsiang-fu Kung ,  Peter Huang ,  Henry I. Huang ,  Paul L. Huang

IPC分类号： A61K36/42 ,  A61K38/00 ,  A61P31/12 ,  A61P35/00 ,  C07K1/00 ,  C07K14/415 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/29 ,  C12P21/02 ,  C12R1/91

CPC分类号： C07K14/415 ,  A61K38/00

摘要： A protein, in particular MAP 30, obtainable from both the fruit and seeds of Momordica charantia or produced by recombinant means useful for treating tumors and HIV infections is disclosed. In treating HIV infections, the protein is administered alone or in conjunction with conventional AIDS therapies. Also provided are processes for purifying the protein, DNA sequences encoding the protein, and recombinant DNA methods for expressing the protein.

摘要翻译： 公开了可从苦瓜属的水果和种子中获得的蛋白质，特别是MAP 30，或通过用于治疗肿瘤和HIV感染的重组手段产生的蛋白质。 在治疗艾滋病毒感染时，该蛋白质单独或与常规艾滋病治疗联合施用。 还提供了纯化蛋白质的方法，编码蛋白质的DNA序列和用于表达蛋白质的重组DNA方法。

公开(公告)号：US5532214A

公开(公告)日：1996-07-02

申请号：US275327

申请日：1994-07-14

申请人： Sylvia Lee-Huang ,  Philip L. Huang ,  Hao-chia Chen ,  Hsiang-fu Kung ,  Peter Huang ,  Henry I. Huang ,  Paul L. Huang

发明人： Sylvia Lee-Huang ,  Philip L. Huang ,  Hao-chia Chen ,  Hsiang-fu Kung ,  Peter Huang ,  Henry I. Huang ,  Paul L. Huang

IPC分类号： A61K31/70 ,  A61K36/42 ,  A61K38/00 ,  A61P31/12 ,  A61P43/00 ,  C07K1/14 ,  C07K14/00 ,  C07K14/415 ,  C07K16/00 ,  C07K16/16 ,  C12N15/09 ,  C12N15/29 ,  C12P21/02 ,  A61K38/02

CPC分类号： C07K16/16 ,  C07K14/415 ,  A61K38/00

摘要： A new protein, termed TAP 29, obtainable from the root tuber of the plant Trichosanthes kirilowii or produced by recombinant means is useful for treating HIV infections or tumors. In treating HIV infections, the protein is administered alone or in conjunction with conventional AIDS therapies. Also provided are processes for purifying the protein, DNA sequences encoding the protein, hosts expressing the protein, recombinant DNA methods for expressing the protein, and antibodies specific for the protein.

摘要翻译： 称为TAP 29的新蛋白质可从植物Trichosanthes kirilowi​​i的根块获得或通过重组方法产生，可用于治疗HIV感染或肿瘤。 在治疗艾滋病毒感染时，该蛋白质单独或与常规艾滋病治疗联合施用。 还提供了纯化蛋白质的方法，编码蛋白质的DNA序列，表达蛋白质的宿主，表达蛋白质的重组DNA方法和对蛋白质特异的抗体。

公开(公告)号：US5317009A

公开(公告)日：1994-05-31

申请号：US749541

申请日：1991-08-26

申请人： Sylvia Lee-Huang ,  Hsiang-fu Kung ,  Paul L. Huang ,  Philip L. Huang ,  Peter Huang ,  Henry I. Huang ,  Hao-chia Chen

发明人： Sylvia Lee-Huang ,  Hsiang-fu Kung ,  Paul L. Huang ,  Philip L. Huang ,  Peter Huang ,  Henry I. Huang ,  Hao-chia Chen

IPC分类号： A61K38/00 ,  A61K39/395 ,  A61K47/48 ,  A61P31/12 ,  A61P31/18 ,  C07K1/18 ,  C07K1/22 ,  C07K1/30 ,  C07K14/415 ,  C07K16/00 ,  C07K16/16 ,  C12N15/09 ,  C12P21/02 ,  C07K13/00 ,  C07K15/10 ,  C07K15/14

CPC分类号： C07K14/415 ,  A61K47/48469 ,  A61K47/48523 ,  A61K38/00 ,  Y10S514/885

摘要： New proteins, termed GAP 31, obtainable from the seeds of Gelonium multiflorum, and DAP 30 and DAP 32, obtainable from the leaves or seeds of Dianthus caryophyllus, or the above proteins produced by recombinant means, are useful for treating HIV infections. In treating HIV infections, the protein is administered alone or in conjunction with other anti-HIV therapeutics. Also provided are processes for purifying the proteins, DNA sequences encoding the proteins, hosts expressing the proteins, recombinant DNA methods for expressing the proteins, and antibodies specific for the proteins.

摘要翻译： 称为GAP 31的新蛋白质，可从多孔多糖种子获得，DAP 30和DAP 32可从石竹属叶子或种子得到，或通过重组方法产生的上述蛋白质可用于治疗HIV感染。 在治疗艾滋病毒感染时，该蛋白质单独施用或与其他抗HIV治疗剂一起施用。 还提供了纯化蛋白质的方法，编码蛋白质的DNA序列，表达蛋白质的宿主，表达蛋白质的重组DNA方法和对蛋白质特异的抗体。

公开(公告)号：US06630347B1

公开(公告)日：2003-10-07

申请号：US09692056

申请日：2000-10-20

申请人： Paul L. Huang ,  Mark C. Fishman ,  Michael A. Moskowitz

发明人： Paul L. Huang ,  Mark C. Fishman ,  Michael A. Moskowitz

IPC分类号： C12N500

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0375 ,  C12N9/0075 ,  C12N9/0077

摘要： This invention relates to transgenic non-human animals comprising a disrupted endothelial nitric oxide synthase gene. These animals exhibit abnormal wound-healing properties and hypertension. This invention also relates to methods of using the transgenic animals to screen for compounds having a potential therapeutic utility for vascular endothelial disorders, such as hypertension, cerebral ischemia or stroke, atherosclerosis and wound-healing activities. Moreover, this invention also relates to methods of treating a patient suffering from hypertension and wound-healing abnormalities with the compounds identified using the transgenic animals, and methods of making the transgenic animals. A method of treating a wound using nitroglycerin is also provided.

摘要翻译： 本发明涉及包含破坏的内皮一氧化氮合酶基因的转基因非人动物。 这些动物表现出异常的伤口愈合特性和高血压。 本发明还涉及使用转基因动物筛选具有血管内皮疾病（例如高血压，脑缺血或中风），动脉粥样硬化和伤口愈合活性的潜在治疗效用的化合物的方法。 此外，本发明还涉及使用转基因动物鉴定的化合物治疗患有高血压和伤口愈合异常的患者的方法，以及制备转基因动物的方法。 还提供了使用硝酸甘油治疗伤口的方法。

公开(公告)号：US06310270B1

公开(公告)日：2001-10-30

申请号：US08818082

申请日：1997-03-14

申请人： Paul L. Huang ,  Mark C. Fishman ,  Michael A. Moskowitz

发明人： Paul L. Huang ,  Mark C. Fishman ,  Michael A. Moskowitz

IPC分类号： A01K6700

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0375 ,  C12N9/0075 ,  C12N9/0077

摘要： This invention relates to transgenic non-human animals comprising a disrupted endothelial nitric oxide synthase gene. These animals exhibit abnormal wound-healing properties and hypertension. This invention also relates to methods of using the transgenic animals to screen for compounds having a potential therapeutic utility for vascular endothelial disorders, such as hypertension, cerebral ischemia or stroke, atherosclerosis and wound-healing activities. Moreover, this invention also relates to methods of treating a patient suffering from hypertension and wound-healing abnormalities with the compounds identified using the transgenic animals, and methods of making the transgenic animals. A method of treating a wound using nitroglycerin is also provided.

摘要翻译： 本发明涉及包含破坏的内皮一氧化氮合酶基因的转基因非人动物。 这些动物表现出异常的伤口愈合特性和高血压。 本发明还涉及使用转基因动物筛选具有血管内皮疾病（例如高血压，脑缺血或中风），动脉粥样硬化和伤口愈合活性的潜在治疗效用的化合物的方法。 此外，本发明还涉及使用转基因动物鉴定的化合物治疗患有高血压和伤口愈合异常的患者的方法，以及制备转基因动物的方法。 还提供了使用硝酸甘油治疗伤口的方法。