公开(公告)号：US20160024523A1

公开(公告)日：2016-01-28

申请号：US14775930

申请日：2014-03-14

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Yan-fang Fu ,  Morgan Maeder

IPC: C12N15/85 ,  C12N9/22

CPC classification number: C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).

Abstract translation: CRISPR-Cas基因组编辑使用指南RNA，其包括通过碱基配对结合靶DNA的互补区和Cas9结合区，将Cas9核酸酶引导至靶DNA。 进一步公开的是通过使用截短的引导RNA（tru-gRNA）增加使用CRISPR / Cas9系统的RNA指导基因组编辑的特异性的方法。

公开(公告)号：US10119133B2

公开(公告)日：2018-11-06

申请号：US14775930

申请日：2014-03-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Yan-fang Fu ,  Morgan Maeder

IPC: C07H21/02 ,  C12N15/00 ,  A61K48/00 ,  C12N15/10 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01

Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).