公开(公告)号：US10190083B2

公开(公告)日：2019-01-29

申请号：US15140118

申请日：2016-04-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Elliot En-Yu Hui ,  Jiang Li ,  Monica Young Kim ,  Allison Lee Curtis

IPC: B05D1/32 ,  C12M1/32 ,  C12M1/00 ,  C12M1/26 ,  C12M1/42 ,  C12N1/00

Abstract: A device for interfacing coatings, the device comprising at least one well each comprising an elastomeric bottom surface. At least one slit is formed on the elastomeric bottom surface. At least one removable divider is removably inserted into the at least one slit, whereby at least one gap is created and the at least one well is divided into at least two compartments. A designated coating is lined on the elastomeric bottom surface of each of the at least two compartments. Removal of the at least one removable divider then causes the at least one gap to close, allowing for the designated coatings lined on the elastomeric bottom surface of each of the at least two compartments to interface with each other.

公开(公告)号：US11427798B2

公开(公告)日：2022-08-30

申请号：US16868474

申请日：2020-05-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: B01L3/00 ,  C12M1/00 ,  G01N1/28 ,  C12M1/40 ,  C12M3/00 ,  C12M1/33

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

公开(公告)号：US10683480B2

公开(公告)日：2020-06-16

申请号：US15413201

申请日：2017-01-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: C40B60/14 ,  C12M1/00 ,  G01N1/28 ,  C12M1/40 ,  C12M3/00 ,  B01L3/00 ,  C12M1/33

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

公开(公告)号：US20200283723A1

公开(公告)日：2020-09-10

申请号：US16868474

申请日：2020-05-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: C12M1/00 ,  G01N1/28 ,  C12M1/40 ,  C12M3/00 ,  B01L3/00

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

公开(公告)号：US09580678B2

公开(公告)日：2017-02-28

申请号：US14309720

申请日：2014-06-19

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: A61B10/00 ,  C12M1/00 ,  C12M1/40 ,  C12M3/00 ,  B01L3/00 ,  G01N1/28 ,  C12M1/33

CPC classification number: G01N1/286 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M21/08 ,  C12M21/18 ,  C12M45/02 ,  C12M47/04

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

Abstract translation: 组织解离装置包括连接到具有具有上游端和下游端的单个通道的第一级的入口; 多个串联布置的中间级，其中多个第一中间级流体耦合到第一级的下游端，并且其中多个的每个后续中间级具有越来越多的通道（具有较小尺寸的通道） ; 以及连接到中间级的最后阶段的出口。

公开(公告)号：US20160310989A1

公开(公告)日：2016-10-27

申请号：US15140118

申请日：2016-04-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Elliot En-Yu Hui ,  Jiang Li ,  Monica Young Kim ,  Allison Lee Curtis

IPC: B05D1/32 ,  B05D3/00

CPC classification number: C12M23/12 ,  C12M23/20 ,  C12M33/00 ,  C12M35/08

Abstract: A device for interfacing coatings, the device comprising at least one well each comprising an elastomeric bottom surface. At least one slit is formed on the elastomeric bottom surface. At least one removable divider is removably inserted into the at least one slit, whereby at least one gap is created and the at least one well is divided into at least two compartments. A designated coating is lined on the elastomeric bottom surface of each of the at least two compartments. Removal of the at least one removable divider then causes the at least one gap to close, allowing for the designated coatings lined on the elastomeric bottom surface of each of the at least two compartments to interface with each other.

Abstract translation: 一种用于连接涂层的装置，所述装置包括至少一个井，每个井包括弹性底面。 在弹性体底表面上形成至少一个狭缝。 至少一个可拆卸的分隔件可移除地插入到至少一个狭缝中，由此产生至少一个间隙，并且至少一个孔被分成至少两个隔室。 指定的涂层衬在所述至少两个隔室中的每一个的弹性体底表面上。 移除所述至少一个可移除的分隔物然后导致所述至少一个间隙闭合，从而允许排列在所述至少两个隔室中的每一个的所述弹性体底表面上的指定涂层彼此接合。

公开(公告)号：US20140377866A1

公开(公告)日：2014-12-25

申请号：US14309720

申请日：2014-06-19

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: C12N5/09 ,  C12M1/40 ,  C12M1/00 ,  G01N1/28

CPC classification number: G01N1/286 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M21/08 ,  C12M21/18 ,  C12M45/02 ,  C12M47/04

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

Abstract translation: 组织解离装置包括连接到具有具有上游端和下游端的单个通道的第一级的入口; 多个串联布置的中间级，其中多个第一中间级流体耦合到第一级的下游端，并且其中多个的每个后续中间级具有越来越多的通道（具有较小尺寸的通道） ; 以及连接到中间级的最后阶段的出口。

公开(公告)号：US20170131187A1

公开(公告)日：2017-05-11

申请号：US15413201

申请日：2017-01-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Janice De Jesus ,  Elliot En-Yu Hui ,  Jiang Li

IPC: G01N1/28

Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.