公开(公告)号：US5399669A

公开(公告)日：1995-03-21

申请号：US96842

申请日：1993-07-26

申请人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

发明人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

IPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  C07K13/00

CPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  Y10S435/849 ,  Y10S930/141

摘要： Recombinantly produced interleukin-2 exhibits human IL-2 activity, has a molecular weight of about 15,000 daltons, is activity stable at a pH of 2-9 and is resistant to elevated temperatures. The recombinant IL-2 has the principal biological activity of human IL-2, promotion of proliferation of cytotoxic T lymphocytes.

摘要翻译： 重组产生的白细胞介素-2显示人IL-2活性，分子量约为15,000道尔顿，在2-9的pH下是活性稳定的并且耐高温。 重组IL-2具有人IL-2的主要生物活性，促进细胞毒性T淋巴细胞增殖。

公开(公告)号：US5700913A

公开(公告)日：1997-12-23

申请号：US331146

申请日：1994-10-28

申请人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

发明人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

IPC分类号： C07K14/55 ,  C12N15/26

CPC分类号： C07K14/55

摘要： Recombinant human Interleukin-2 (IL-2) polypeptides are provided. The polypeptides are produced by expression of suitable nucleic acid molecules in transformed host cells such as E. coli.

摘要翻译： 提供重组人白介素-2（IL-2）多肽。 通过在转化的宿主细胞如大肠杆菌中表达合适的核酸分子来产生多肽。

公开(公告)号：US5620868A

公开(公告)日：1997-04-15

申请号：US814049

申请日：1991-12-26

申请人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

发明人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

IPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  C12P21/00 ,  C12N15/00 ,  C12P21/02

CPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  Y10S435/849 ,  Y10S930/141

摘要： A gene coded for a polypeptide which possesses interleukin-2 is isolated, and connected with a vector DNA which is capable of replicating in a procaryotic or eucaryotic cell at a position downstream of a promoter gene in the vector obtaining a recombinant DNA, with which the cell is transformed to produce interleukin-2.

摘要翻译： 分离编码具有白细胞介素-2的多肽的基因，并与载体DNA连接，该载体DNA能够在原核或真核细胞中在载体中的启动子基因下游位置复制获得重组DNA， 转化细胞以产生白介素-2。

公开(公告)号：US5795777A

公开(公告)日：1998-08-18

申请号：US621097

申请日：1996-03-22

申请人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

发明人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

IPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  C12N5/00 ,  C12N15/26

CPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  Y10S435/849 ,  Y10S930/141

摘要： A gene coded for a polypeptide which possesses interleukin-2 is isolated, and connected with a vector DNA which is capable of replicating in a procaryotic or eucaryotic cell at a position downstream of a promoter gene in the vector obtaining a recombinant DNA, with which the cell is trans-formed to produce interleukin-2.

摘要翻译： 分离编码具有白细胞介素-2的多肽的基因，并与载体DNA连接，该载体DNA能够在原核或真核细胞中在载体中的启动子基因下游位置复制获得重组DNA， 细胞被转化以产生白细胞介素-2。

公开(公告)号：US4738927A

公开(公告)日：1988-04-19

申请号：US463496

申请日：1983-02-03

申请人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

发明人： Tadatsugu Taniguchi ,  Masami Muramatsu ,  Haruo Sugano ,  Hiroshi Matsui ,  Nobukazu Kashima ,  Junji Hamuro

IPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  C12N1/00 ,  C07H21/04 ,  C07K13/00 ,  C12N1/16 ,  C12N1/18 ,  C12N1/20 ,  C12N5/00 ,  C12N5/02 ,  C12N15/00 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12P21/04 ,  C12R1/19

CPC分类号： C07K14/55 ,  C12N15/70 ,  C12N15/81 ,  Y10S435/849 ,  Y10S930/141

摘要： A gene coded for a polypeptide which possesses interleukin-2 activity is isolated, and connected with a vector DNA which is capable of replicating in a procaryotic or eucaryotic cell at a position downstream of a promoter gene in the vector obtaining a recombinant DNA, with which the cell is transformed to produce interleukin-2.

摘要翻译： 分离编码具有白细胞介素-2活性的多肽的基因，并与载体DNA连接，该载体DNA能够在原核或真核细胞中在载体中的启动子基因下游的位置复制获得重组DNA， 转化细胞以产生白介素-2。

公开(公告)号：US4789658A

公开(公告)日：1988-12-06

申请号：US873302

申请日：1986-06-09

申请人： Ryota Yoshimoto ,  Nobukazu Kashima ,  Junji Hamuro ,  Koji Mitsugi

发明人： Ryota Yoshimoto ,  Nobukazu Kashima ,  Junji Hamuro ,  Koji Mitsugi

IPC分类号： C12N15/02 ,  A61K35/12 ,  A61K35/14 ,  A61K35/28 ,  A61K36/06 ,  A61K38/00 ,  A61K38/16 ,  A61K38/18 ,  A61P35/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/55 ,  C07K14/555 ,  C07K14/56 ,  C12P21/00 ,  A61K37/00

CPC分类号： C07K14/55 ,  A61K31/716 ,  A61K38/21 ,  A61K38/00

摘要： An immunopropylactic and immunotherapeutic agent comprising human interleukin 2 of human cellular origin is disclosed along with a method of producing the agent.

摘要翻译： 公开了包含人细胞来源的人白细胞介素2的免疫性丙立体和免疫治疗剂及其制备方法。

公开(公告)号：US20060252700A1

公开(公告)日：2006-11-09

申请号：US11484605

申请日：2006-07-12

申请人： Junji Hamuro ,  Yukie Murata

发明人： Junji Hamuro ,  Yukie Murata

IPC分类号： A61K38/05 ,  A61K31/353 ,  C12Q1/00 ,  A61K31/385 ,  G06F19/00

CPC分类号： A61K31/353 ,  A23L33/175 ,  A61K31/225 ,  A61K31/385 ,  A61K38/05 ,  A61K38/063 ,  A61K38/2013 ,  A61K47/62 ,  A61K2300/00

摘要： An immunomodulator is provided which is capable of oral intake for improvement, treatment and prevention of human immunological diseases and which is used to treat, improve and prevent human immunological diseases, especially, autoimmune diseases and allergic diseases such as hepatic cirrhosis, hepatitis, diabetes, inflammatory bowel diseases, chronic rheumatoid arthritis, asthma and cutaneous atopy, allergic diseases and cancers by a new method that can control the redox state of macrophages or monocytes, and can be incorporated into a drug, a food, a nutrient, and an infusion. The contents of oxidative glutathione and reductive glutathione in macrophages are monitored, and the ratio of oxidative glutathione and reductive glutathione is examined, whereby macrophages are classified into oxidative macrophages and reductive macrophages having different functions. The degree of progression of various autoimmune diseases is analyzed from this standpoint. On the basis of the results, an immunomodulator capable of oral intake which contains a substance having an activity of changing a content of reductive glutathione in macrophages to solve the above-mentioned problem and which is intended to treat, improve and prevent human immune diseases is provided.

公开(公告)号：US06444800B1

公开(公告)日：2002-09-03

申请号：US09348265

申请日：1999-07-07

申请人： Kokichi Kikuchi ,  Noriyuki Sato ,  Toshihiko Torigoe ,  Hiroeki Sahara ,  Manabu Suzuki ,  Junji Hamuro

发明人： Kokichi Kikuchi ,  Noriyuki Sato ,  Toshihiko Torigoe ,  Hiroeki Sahara ,  Manabu Suzuki ,  Junji Hamuro

IPC分类号： C07H2102

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00

摘要： A tumor antigen gene is identified by screening a cDNA library derived from a gastric cancer cell line that can induce gastric cancer antigen specific cytotoxic T cell (CTL) by means of hybridization and PCR utilizing an amino acid sequence of peptide fragment of a known gastric cancer antigen protein, introducing a selected cDNA clone into a cell of gastric cancer cell line that cannot induce gastric cancer antigen specific CTL so that the clone should be expressed in the cell, and selecting a transgenic cell that has acquired the ability to induce CTL. According to the present invention, there are provided a protein capable of inducing immune response against human gastric cancer, DNA encoding the protein, as well as vaccine for treatment and prevention of human gastric cancer, and agent for treatment and prevention of human gastric cancer.

公开(公告)号：US5856140A

公开(公告)日：1999-01-05

申请号：US636936

申请日：1996-04-24

申请人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

发明人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

IPC分类号： A61K38/00 ,  C07K14/715 ,  C07K16/28 ,  C12N1/21 ,  C12N15/13 ,  C12P21/08 ,  C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K14/7155 ,  C07K16/2866 ,  A61K38/00

摘要： A polypeptide which specifically binds to the .gamma.-chain of the human interleukin-2 receptor and selectively inhibits the binding of the .gamma.-chain of human interleukin-2 receptor to the .beta.-chain of the same is provided. The polypeptide has an activity of blocking the human interleukin-2 response. Also provided are an immunosuppressant containing the polypeptide, a DNA gene coding for the polypeptide, a recombinant DNA having the gene, a transformant having the recombinant DNA, and a method for producing the intended polypeptide by incubating the transformant. The novel polypeptide can be used independently, or with substances capable of inhibiting the binding of interleukin-2 to the interleukin-2 receptor, as a medicine for preventing the rejection of grafts after transplantation and also for curing inflammatory diseases such as allergic diseases and autoimmune diseases.

摘要翻译： 提供了特异性结合人白细胞介素-2受体的γ-链的多肽，并选择性地抑制人白细胞介素-2受体的γ-链与β-链的结合。 该多肽具有阻断人白介素-2应答的活性。 还提供了含有多肽的免疫抑制剂，编码该多肽的DNA基因，具有该基因的重组DNA，具有该重组DNA的转化体，以及通过温育该转化体来制备该多肽的方法。 该新型多肽可以独立使用，也可以与能够抑制白介素-2与白细胞介素-2受体结合的物质用作防止移植后移植物排斥的药物，还可用于治疗炎性疾病如过敏性疾病和自身免疫性 疾病

公开(公告)号：US5801003A

公开(公告)日：1998-09-01

申请号：US568427

申请日：1995-12-06

申请人： Toshiro Shimamura ,  Junji Hamuro ,  Kazuo Sugamura

发明人： Toshiro Shimamura ,  Junji Hamuro ,  Kazuo Sugamura

IPC分类号： G01N33/53 ,  C07K16/28 ,  G01N33/564 ,  G01N33/574 ,  G01N33/577 ,  G01N33/68 ,  C12Q1/70 ,  G01N33/543

CPC分类号： C07K16/2866 ,  G01N33/6869

摘要： The invention provides a method for detecting (1) leukemia, (2) cancer, (3) autoimmune disorder, (4) inflammatory disorder, (5) allergic disorder or (6) viral infection, characterized by measuring soluble, human interleukin-2 receptor .gamma.-chain molecules existing in a human body fluid by an immunochemical method using an antibody/antibodies specifically reacting with human interleukin-2 receptor .gamma.-chain molecule, and a reagent for the detection.

摘要翻译： 本发明提供一种检测白血病，（2）癌症，（3）自身免疫性疾病，（4）炎症性疾病，（5）过敏性疾病或（6）病毒感染的方法，其特征在于测量可溶性人白介素-2 通过使用与人白细胞介素-2受体γ链分子特异性反应的抗体/抗体的免疫化学方法和用于检测的试剂，存在于人体液中的受体γ链分子。