公开(公告)号：US5856140A

公开(公告)日：1999-01-05

申请号：US636936

申请日：1996-04-24

申请人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

发明人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

IPC分类号： A61K38/00 ,  C07K14/715 ,  C07K16/28 ,  C12N1/21 ,  C12N15/13 ,  C12P21/08 ,  C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K14/7155 ,  C07K16/2866 ,  A61K38/00

摘要： A polypeptide which specifically binds to the .gamma.-chain of the human interleukin-2 receptor and selectively inhibits the binding of the .gamma.-chain of human interleukin-2 receptor to the .beta.-chain of the same is provided. The polypeptide has an activity of blocking the human interleukin-2 response. Also provided are an immunosuppressant containing the polypeptide, a DNA gene coding for the polypeptide, a recombinant DNA having the gene, a transformant having the recombinant DNA, and a method for producing the intended polypeptide by incubating the transformant. The novel polypeptide can be used independently, or with substances capable of inhibiting the binding of interleukin-2 to the interleukin-2 receptor, as a medicine for preventing the rejection of grafts after transplantation and also for curing inflammatory diseases such as allergic diseases and autoimmune diseases.

摘要翻译： 提供了特异性结合人白细胞介素-2受体的γ-链的多肽，并选择性地抑制人白细胞介素-2受体的γ-链与β-链的结合。 该多肽具有阻断人白介素-2应答的活性。 还提供了含有多肽的免疫抑制剂，编码该多肽的DNA基因，具有该基因的重组DNA，具有该重组DNA的转化体，以及通过温育该转化体来制备该多肽的方法。 该新型多肽可以独立使用，也可以与能够抑制白介素-2与白细胞介素-2受体结合的物质用作防止移植后移植物排斥的药物，还可用于治疗炎性疾病如过敏性疾病和自身免疫性 疾病

公开(公告)号：US5582826A

公开(公告)日：1996-12-10

申请号：US230843

申请日：1994-04-21

申请人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

发明人： Toshiro Shimamura ,  Junji Hamuro ,  Harumi Nakazawa ,  Yuka Kanayama ,  Kazuo Sugamura ,  Toshikazu Takeshita

IPC分类号： A61K38/00 ,  C07K14/715 ,  C07K16/28 ,  C12N1/21 ,  C12N15/13 ,  C12P21/08 ,  A61K39/395 ,  C07K16/24 ,  C12N5/12

CPC分类号： C07K14/7155 ,  C07K16/2866 ,  A61K38/00

摘要： A polypeptide which specifically binds to the .gamma.-chain of the human interleukin-2 receptor and selectively inhibits the binding of the .gamma.-chain of human interleukin-2 receptor to the .beta.-chain of the same is provided. The polypeptide has an activity of blocking the human interleukin-2 response. Also provided are an immunosuppressant containing the polypeptide, a DNA gene coding for the polypeptide, a recombinant DNA having the gene, a transformant having the recombinant DNA, and a method for producing the intended polypeptide by incubating the transformant. The novel polypeptide can be used independently, or with substances capable of inhibiting the binding of interleukin-2 to the interleukin-2 receptor, as a medicine for preventing the rejection of grafts after transplantation and also for curing inflammatory diseases such as allergic diseases and autoimmune diseases.

摘要翻译： 提供了特异性结合人白细胞介素-2受体的γ-链的多肽，并选择性地抑制人白细胞介素-2受体的γ-链与β-链的结合。 该多肽具有阻断人白介素-2应答的活性。 还提供了含有多肽的免疫抑制剂，编码该多肽的DNA基因，具有该基因的重组DNA，具有该重组DNA的转化体，以及通过温育该转化体来制备该多肽的方法。 该新型多肽可以独立使用，也可以与能够抑制白介素-2与白细胞介素-2受体结合的物质用作防止移植后移植物排斥的药物，还可用于治疗炎性疾病如过敏性疾病和自身免疫性 疾病

公开(公告)号：US5705608A

公开(公告)日：1998-01-06

申请号：US595974

申请日：1996-02-06

申请人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

发明人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61P37/00 ,  C07K1/20 ,  C07K1/22 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/577 ,  C07K1/00

CPC分类号： C07K14/7155 ,  A61K38/00

摘要： The present invention relates to an IL-2 receptor .gamma. chain molecule, a DNA-sequence encoding the IL-2 receptor .gamma. chain molecule, a vector possessing said DNA-sequence, a cell transformed with said vector, a method for the production of an IL-2 receptor .gamma. chain molecule by culturing of said cell, an immune response regulatory agent comprising an Il-2 receptor .gamma. chain molecule and an antibody to an IL-2 receptor .gamma. chain molecule. Both the Il-2 receptor .gamma. chain molecule and the antibody to the IL-2 receptor .gamma. chain molecule are very useful immune response regulatory agents.

公开(公告)号：US5889160A

公开(公告)日：1999-03-30

申请号：US902929

申请日：1997-07-30

申请人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

发明人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61P37/00 ,  C07K1/20 ,  C07K1/22 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/577 ,  G01N33/566

CPC分类号： C07K14/7155 ,  A61K38/00

摘要： The present invention relates to an IL-2 receptor .gamma. chain molecule, a DNA-sequence encoding the IL-2 receptor .gamma. chain molecule, a vector possessing said DNA-sequence, a cell transformed with said vector, a method for the production of an IL-2 receptor .gamma. chain molecule by culturing of said cell, an immune response regulatory agent comprising an IL-2 receptor .gamma. chain molecule and an antibody to an IL-2 receptor .gamma. chain molecule.

摘要翻译： 本发明涉及IL-2受体γ链分子，编码IL-2受体γ链分子的DNA序列，具有所述DNA序列的载体，用所述载体转化的细胞，生产 IL-2受体γ链分子通过培养所述细胞，包含IL-2受体γ链分子和针对IL-2受体γ链分子的抗体的免疫应答调节剂。

公开(公告)号：US5510259A

公开(公告)日：1996-04-23

申请号：US52205

申请日：1993-04-22

申请人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

发明人： Kazuo Sugamura ,  Toshikazu Takeshita ,  Hironobu Asao ,  Masataka Nakamura ,  Toshiro Shimamura ,  Manabu Suzuki ,  Junji Hamuro

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61P37/00 ,  C07K1/20 ,  C07K1/22 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/577 ,  C12N5/00 ,  C07H21/04 ,  C12N15/00

CPC分类号： C07K14/7155 ,  A61K38/00

摘要： The present invention relates to an IL-2 receptor .gamma. chain molecule, a DNA-sequence encoding the IL-2 receptor .gamma. chain molecule, a vector possessing said DNA-sequence, a cell transformed with said vector, a method for the production of an IL-2 receptor .gamma. chain molecule by culturing of said cell, an immune response regulatory agent comprising an Il-2 receptor .gamma. chain molecule and an antibody to an IL-2 receptor .gamma. chain molecule.Both the Il-2 receptor .gamma. chain molecule and the antibody to the IL-2 receptor .gamma. chain molecule are very useful immune response regulatory agents.

摘要翻译： 本发明涉及IL-2受体γ链分子，编码IL-2受体γ链分子的DNA序列，具有所述DNA序列的载体，用所述载体转化的细胞，生产 IL-2受体γ链分子通过培养所述细胞，包含Il-2受体γ链分子和针对IL-2受体γ链分子的抗体的免疫应答调节剂。 IL-2受体γ链分子和IL-2受体γ链分子的抗体都是非常有用的免疫应答调节剂。

公开(公告)号：US5801003A

公开(公告)日：1998-09-01

申请号：US568427

申请日：1995-12-06

申请人： Toshiro Shimamura ,  Junji Hamuro ,  Kazuo Sugamura

发明人： Toshiro Shimamura ,  Junji Hamuro ,  Kazuo Sugamura

IPC分类号： G01N33/53 ,  C07K16/28 ,  G01N33/564 ,  G01N33/574 ,  G01N33/577 ,  G01N33/68 ,  C12Q1/70 ,  G01N33/543

CPC分类号： C07K16/2866 ,  G01N33/6869

摘要： The invention provides a method for detecting (1) leukemia, (2) cancer, (3) autoimmune disorder, (4) inflammatory disorder, (5) allergic disorder or (6) viral infection, characterized by measuring soluble, human interleukin-2 receptor .gamma.-chain molecules existing in a human body fluid by an immunochemical method using an antibody/antibodies specifically reacting with human interleukin-2 receptor .gamma.-chain molecule, and a reagent for the detection.

摘要翻译： 本发明提供一种检测白血病，（2）癌症，（3）自身免疫性疾病，（4）炎症性疾病，（5）过敏性疾病或（6）病毒感染的方法，其特征在于测量可溶性人白介素-2 通过使用与人白细胞介素-2受体γ链分子特异性反应的抗体/抗体的免疫化学方法和用于检测的试剂，存在于人体液中的受体γ链分子。

公开(公告)号：US08541033B2

公开(公告)日：2013-09-24

申请号：US12620469

申请日：2009-11-17

申请人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

发明人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

IPC分类号： A61K35/28 ,  C12P21/00 ,  A01K67/00

CPC分类号： C07K16/18 ,  A01K67/027 ,  A01K67/0271 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0337 ,  A61K39/00

摘要： The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor γ-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

摘要翻译： 本发明提供了适合于异源细胞的移植，分化和增殖的免疫缺陷小鼠（NOG小鼠），以及这种小鼠的制备方法。 该小鼠通过用NOD / Shi小鼠回交C.B-17- scid小鼠并通过回交的小鼠进一步回交白细胞介素2受体γ链基因敲除小鼠而获得。 它可用于产生人抗体并建立干细胞测定系统，肿瘤模型和病毒感染模型。

公开(公告)号：US20070011753A1

公开(公告)日：2007-01-11

申请号：US11517698

申请日：2006-09-08

申请人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

发明人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

IPC分类号： A01K67/027

CPC分类号： C07K16/18 ,  A01K67/027 ,  A01K67/0271 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0337 ,  A61K39/00

摘要： The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor γ-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

摘要翻译： 本发明提供了适合于异源细胞的移植，分化和增殖的免疫缺陷小鼠（NOG小鼠），以及这种小鼠的制备方法。 该小鼠通过用NOD / Shi小鼠回交C.B-17- scid小鼠并通过回交的小鼠进一步回交白细胞介素2受体γ链基因敲除小鼠而获得。 它可用于产生人抗体并建立干细胞测定系统，肿瘤模型和病毒感染模型。

公开(公告)号：US07145055B2

公开(公告)日：2006-12-05

申请号：US10221549

申请日：2001-10-25

申请人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

发明人： Mamoru Ito ,  Kimio Kobayashi ,  Tatsutoshi Nakahata ,  Koichiro Tsuji ,  Sonoko Habu ,  Yoshio Koyanagi ,  Naoki Yamamoto ,  Kazuo Sugamura ,  Kiyoshi Ando ,  Tatsuji Nomura

IPC分类号： G01N33/00 ,  C12N15/01 ,  A01K67/02 ,  A01K67/027

CPC分类号： C07K16/18 ,  A01K67/027 ,  A01K67/0271 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0337 ,  A61K39/00

摘要： The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor γ-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

摘要翻译： 本发明提供了适合于异源细胞的移植，分化和增殖的免疫缺陷小鼠（NOG小鼠），以及这种小鼠的制备方法。 该小鼠通过用NOD / Shi小鼠回交C.B-17- scid小鼠并通过回交的小鼠进一步回交白细胞介素2受体γ链基因敲除小鼠而获得。 它可用于产生人抗体并建立干细胞测定系统，肿瘤模型和病毒感染模型。

公开(公告)号：US20050144662A1

公开(公告)日：2005-06-30

申请号：US10942954

申请日：2004-09-17

申请人： Masataka Nakamura ,  Kazuo Sugamura ,  Shoichi Takano ,  Kinya Nagata ,  Hiroyuki Hirai

发明人： Masataka Nakamura ,  Kazuo Sugamura ,  Shoichi Takano ,  Kinya Nagata ,  Hiroyuki Hirai

IPC分类号： A01K67/027 ,  C07K14/705 ,  C12N15/09 ,  C12N15/85 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C07K14/705

摘要： A knockout mouse whose genome includes an inactivated CRTH2 gene. The knockout mouse is obtained by subjecting to a serial passage a chimeric mouse originating from an early embryo to which a CRTH2-gene-knocked-out mouse embryonic stem cell has been introduced. Also disclosed is a detection method which includes employing, as an index, pathological condition of the knockout mouse to which the test substance has been administered, to thereby detect, in vivo, characteristics of a test substance in relation to CRTH2, or functions of CRTH2 in the living body.

摘要翻译： 基因组的敲除小鼠，其包含失活的CRTH2基因。 敲除小鼠通过连续通过源自已经引入了CRTH2基因敲除的小鼠胚胎干细胞的早期胚胎的嵌合小鼠获得。 还公开了一种检测方法，其包括使用已经施用受检物质的敲除小鼠的病理状态作为指标，从而检测体内与CRTH2相关的测试物质的特征，或CRTH2的功能 在活体内。