利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

36 条记录 (耗时 0.022 秒)

    Method for producing a biosensor
    1.
    发明申请
    Method for producing a biosensor 有权
    生物传感器的制造方法

    公开(公告)号：US20070266775A1

    公开(公告)日：2007-11-22

    申请号：US11710433

    申请日：2007-02-26

    申请人： Taisei Nishimi ,  Koichi Kawamura

    发明人： Taisei Nishimi ,  Koichi Kawamura

    IPC分类号： G01N33/487 ,  B05D5/00 ,  C12Q1/00

    CPC分类号： G01N33/54393

    摘要： Thus, the present invention provides a method for producing a biosensor comprising a substrate coated with a hydrophilic polymer, which comprises allowing a compound generating a reactive group as a result of external stimulus to bind to the surface of a substrate, and then giving external stimulus in a state where a hydrophilic polymer capable of forming a covalent bond with said reactive group is allowed to come into contact with the substrate, so that said hydrophilic polymer is allowed to bind to the substrate surface via a covalent bond with said reactive group.

    摘要翻译： 因此，本发明提供一种生物传感器的制造方法，其包括涂布有亲水性聚合物的基材，其包括使作为外部刺激的结果产生反应性基团的化合物结合到基材的表面，然后进行外部刺激 在能够与所述反应性基团形成共价键的亲水性聚合物与基材接触的状态下，使得所述亲水性聚合物通过与所述反应性基团的共价键与基材表面结合。

    Method for producing a biosensor
    2.
    发明授权
    Method for producing a biosensor 有权
    生物传感器的制造方法

    公开(公告)号：US08580571B2

    公开(公告)日：2013-11-12

    申请号：US11710433

    申请日：2007-02-26

    申请人： Taisei Nishimi ,  Koichi Kawamura

    发明人： Taisei Nishimi ,  Koichi Kawamura

    IPC分类号： G01N33/487 ,  B05D5/00

    CPC分类号： G01N33/54393

    摘要： Thus, the present invention provides a method for producing a biosensor comprising a substrate coated with a hydrophilic polymer, which comprises allowing a compound generating a reactive group as a result of external stimulus to bind to the surface of a substrate, and then giving external stimulus in a state where a hydrophilic polymer capable of forming a covalent bond with said reactive group is allowed to come into contact with the substrate, so that said hydrophilic polymer is allowed to bind to the substrate surface via a covalent bond with said reactive group.

    摘要翻译： 因此，本发明提供一种生物传感器的制造方法，其包括涂布有亲水性聚合物的基材，其包括使作为外部刺激的结果产生反应性基团的化合物结合到基材的表面，然后进行外部刺激 在能够与所述反应性基团形成共价键的亲水性聚合物与基材接触的状态下，使得所述亲水性聚合物通过与所述反应性基团的共价键与基材表面结合。

    Biosensor
    3.
    发明授权
    Biosensor 有权
    生物传感器

    公开(公告)号：US08585972B2

    公开(公告)日：2013-11-19

    申请号：US12778555

    申请日：2010-05-12

    申请人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo ,  Hidetoshi Tomita

    发明人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo ,  Hidetoshi Tomita

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54353 ,  G01N33/54373

    摘要： One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.

    摘要翻译： 本发明的一个目的是提供一种生物传感器及其生产方法，通过该生物传感器及其生产方法，可以使用安全的原料方便地生产能够固定生理活性物质的水凝胶。 本发明提供一种生物传感器，其包括在其表面具有金属层的基材，其中具有能够与生理活性物质的羟基或氨基反应的反应性官能团的亲水性聚合物直接结合到金属层 或间接经由中间层。

    Method for purifying bioactive substances
    4.
    发明授权
    Method for purifying bioactive substances 有权
    生物活性物质的净化方法

    公开(公告)号：US07928191B2

    公开(公告)日：2011-04-19

    申请号：US12201594

    申请日：2008-08-29

    申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

    发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

    IPC分类号： C07K1/16

    CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

    摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

    摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质由10mmol / L至1mol / L的咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

    BIOSENSOR SUBSTRATE
    5.
    发明申请
    BIOSENSOR SUBSTRATE 审中-公开
    生物传感器基板

    公开(公告)号：US20080188010A1

    公开(公告)日：2008-08-07

    申请号：US12022606

    申请日：2008-01-30

    申请人： Yukou Saitoh ,  Toshihide Ezoe ,  Taisei Nishimi

    发明人： Yukou Saitoh ,  Toshihide Ezoe ,  Taisei Nishimi

    IPC分类号： G01N33/551 ,  B01J19/00

    CPC分类号： G01N33/54373

    摘要： It is an object of the present invention to provide a biosensor substrate that prevents non-specific adsorption and is used for immobilizing a physiologically active substance. The present invention provides a biosensor substrate which comprises an inorganic oxide film and a hydrophilic polymer having a physiologically active substance-immobilizing group on the substrate, wherein the hydrophilic polymer is bound to the inorganic oxide film through a low molecular compound.

    摘要翻译： 本发明的目的是提供一种防止非特异性吸附并用于固定生理活性物质的生物传感器基板。 本发明提供一种生物传感器基板，其包含无机氧化物膜和在基板上具有生理活性物质固定基团的亲水性聚合物，其中亲水性聚合物通过低分子化合物与无机氧化物膜结合。

    Biosensor
    6.
    发明申请
    Biosensor 有权
    生物传感器

    公开(公告)号：US20060246512A1

    公开(公告)日：2006-11-02

    申请号：US11377554

    申请日：2006-03-17

    申请人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

    发明人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

    IPC分类号： G01N33/53 ,  C12M1/34

    CPC分类号： C12Q1/002 ,  G01N21/553 ,  G01N21/554 ,  G01N33/5438 ,  Y10S436/805

    摘要： It is an object of the present invention to provide a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The present invention provides a biosensor which comprises a substrate having at least two types of surfaces on a single plane, wherein at least one of said surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to said surface that does not retain a physiologically active substance.

    摘要翻译： 本发明的目的是提供一种生物传感器，其中抑制了不保留生理活性物质（参考单元）的表面上的非特异性吸附。 本发明提供了一种生物传感器，其包括在单个平面上具有至少两种类型的表面的基底，其中所述表面中的至少一个不保留生理活性物质，并且其中至少两种具有不同分子量的亲水性化合物 结合到不保留生理活性物质的所述表面。

    Biosensor
    7.
    发明申请
    Biosensor 审中-公开
    生物传感器

    公开(公告)号：US20060040410A1

    公开(公告)日：2006-02-23

    申请号：US11205183

    申请日：2005-08-17

    申请人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo

    发明人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo

    IPC分类号： C12M1/34 ,  G01N33/553

    CPC分类号： G01N33/54393 ,  G01N33/54373

    摘要： It is an object of the present invention to provide a technique of converting carboxylic acid into an active ester with no generation of air bubbles and a technique of stabilizing the obtained active ester. The present invention provides a biosensor wherein a carboxyl group existing on the surface of a substrate thereof is activated with any one compound selected from an uronium salt, a phosphonium salt or a triazine derivative which are defined in the present application, so as to form a carboxylic acid amide group.

    摘要翻译： 本发明的目的是提供一种不产生气泡而将羧酸转化成活性酯的技术，以及稳定所得活性酯的技术。 本发明提供一种生物传感器，其中存在于其底物表面上的羧基由选自本申请中定义的铀盐，鏻盐或三嗪衍生物的任何一种化合物活化，从而形成 羧酸酰胺基。

    Biosensor with suppressed non-specific adsorption
    9.
    发明授权
    Biosensor with suppressed non-specific adsorption 有权
    抑制非特异性吸附的生物传感器

    公开(公告)号：US07964414B2

    公开(公告)日：2011-06-21

    申请号：US11377554

    申请日：2006-03-17

    申请人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

    发明人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

    IPC分类号： G01N33/543 ,  C12M1/34

    CPC分类号： C12Q1/002 ,  G01N21/553 ,  G01N21/554 ,  G01N33/5438 ,  Y10S436/805

    摘要： The present invention provides a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The biosensor of the invention includes a substrate having at least two types of surfaces on a single plane, wherein at least one of the surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to the surface that does not retain a physiologically active substance.

    摘要翻译： 本发明提供一种生物传感器，其中抑制了不保留生理活性物质（参考单元）的表面上的非特异性吸附。 本发明的生物传感器包括在单个平面上具有至少两种类型的表面的基底，其中至少一个表面不保留生理活性物质，并且其中至少两种具有不同分子量的亲水性化合物被结合 到没有保留生理活性物质的表面。