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公开(公告)号：US20100144677A1
公开(公告)日：2010-06-10
申请号：US12622010
申请日：2009-11-19
申请人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga , Shigeru Yoshida , Yoshitaka Ueda
发明人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga , Shigeru Yoshida , Yoshitaka Ueda
IPC分类号： A61K31/685 , C12Q1/02 , G01N33/53 , A61K31/44 , A61P3/10
CPC分类号： C07K14/705 , A61K38/00 , C07K14/723
摘要： A convenient screening tool and a convenient screening method for obtaining an agent for treating diabetes, a pharmaceutical composition for treating diabetes, and a process for manufacturing the pharmaceutical composition are disclosed.The screening tool is a G protein-coupled receptor, a variation functionally equivalent thereto, or a homologous polypeptide thereof which promotes insulin secretion under a high glucose concentration by activation, or cells transformed with an expression vector comprising a polynucleotide encoding the above polypeptide and expressing the polypeptide.
摘要翻译： 公开了一种方便的筛选工具和用于获得治疗糖尿病的药物的方便的筛选方法，用于治疗糖尿病的药物组合物和制备该药物组合物的方法。 筛选工具是G蛋白偶联受体，功能上等同于其的变异体，或其通过活化促进高葡萄糖浓度下的胰岛素分泌的同源多肽，或用包含编码上述多肽的多核苷酸的表达载体转化的细胞，并表达 多肽。
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公开(公告)号：US20050136484A1
公开(公告)日：2005-06-23
申请号：US10975367
申请日：2004-10-29
申请人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga , Shigeru Yoshida , Yoshitaka Ueda
发明人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga , Shigeru Yoshida , Yoshitaka Ueda
IPC分类号： A61K38/00 , A61P3/10 , A61P43/00 , C07K14/705 , C07K14/72 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/12 , G01N33/53
CPC分类号： C07K14/705 , A61K38/00 , C07K14/723
摘要： A convenient screening tool and a convenient screening method for obtaining an agent for treating diabetes, a pharmaceutical composition for treating diabetes, and a process for manufacturing the pharmaceutical composition are disclosed. The screening tool is a G protein-coupled receptor, a variation functionally equivalent thereto, or a homologous polypeptide thereof which promotes insulin secretion under a high glucose concentration by activation, or cells transformed with an expression vector comprising a polynucleotide encoding the above polypeptide and expressing the polypeptide.
摘要翻译： 公开了一种方便的筛选工具和用于获得治疗糖尿病的药物的方便的筛选方法，用于治疗糖尿病的药物组合物和制备该药物组合物的方法。 筛选工具是G蛋白偶联受体，功能上等同于其的变异体，或其通过活化促进高葡萄糖浓度下的胰岛素分泌的同源多肽，或用包含编码上述多肽的多核苷酸的表达载体转化的细胞，并表达 多肽。
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公开(公告)号：US07662775B2
公开(公告)日：2010-02-16
申请号：US10975367
申请日：2004-10-29
申请人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga
发明人： Takahide Ohishi , Jun Takasaki , Mitsuyuki Matsumoto , Tetsu Saito , Masazumi Kamohara , Takatoshi Soga
CPC分类号： C07K14/705 , A61K38/00 , C07K14/723
摘要： The invention relates to a convenient screening tool for identifying an agent for treating diabetes. The screening tool is a G protein-coupled receptor, a variant of the G protein-coupled receptor, or a homolog of the G protein-coupled receptor, which promotes insulin secretion under a high glucose concentration by activation. The invention also relates to cells transformed with an expression vector comprising a polynucleotide encoding and expressing the screening tool. The invention provides a convenient screening method for identifying an agent for treating diabetes, pharmaceutical compositions comprising the agent, and a process for manufacturing the pharmaceutical composition.
摘要翻译： 本发明涉及用于鉴定用于治疗糖尿病的药剂的方便的筛选工具。 筛选工具是G蛋白偶联受体，G蛋白偶联受体的变体或G蛋白偶联受体的同系物，其通过活化促进高葡萄糖浓度下的胰岛素分泌。 本发明还涉及用包含编码和表达筛选工具的多核苷酸的表达载体转化的细胞。 本发明提供了用于鉴定用于治疗糖尿病的药剂的方便的筛选方法，包含该药剂的药物组合物和用于制备该药物组合物的方法。
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公开(公告)号：US07405051B2
公开(公告)日：2008-07-29
申请号：US10333844
申请日：2001-10-31
申请人： Jun Takasaki , Mitsuyuki Matsumoto , Masazumi Kamohara , Tetsu Saito , Takahide Ohishi , Takatoshi Soga
发明人： Jun Takasaki , Mitsuyuki Matsumoto , Masazumi Kamohara , Tetsu Saito , Takahide Ohishi , Takatoshi Soga
IPC分类号： G01N33/53 , G01N33/567 , G01N33/566 , C12Q1/68 , C12N5/00 , C12N5/06 , C07K1/00
CPC分类号： G01N33/566 , G01N33/6872 , G01N2333/726 , G01N2500/00
摘要： A screening tool for an antiplatelet agent wherein the tool is a human ADP receptor P2TAC protein, a variation functionally equivalent thereto, or a homologous protein thereof, and a screening tool for an antiplatelet agent wherein the tool is a transformant which is transformed with an expression vector comprising a DNA encoding the protein and is expressing the polypeptide are disclosed. Further, a method for detecting an ADP receptor P2TAC ligand, antagonist, or agonist by using the screening tool for an antiplatelet agent, and a method for screening an antiplatelet agent by the detecting method are disclosed.
摘要翻译： 用于抗血小板剂的筛选工具，其中所述工具是人ADP受体P2T蛋白，与其功能等同的变体或其同源蛋白，以及用于抗血小板剂的筛选工具，其中所述工具是 公开了用包含编码该蛋白质并正在表达多肽的DNA的表达载体转化的转化体。 此外，公开了通过使用抗血小板剂的筛选工具检测ADP受体P2TACAC配体，拮抗剂或激动剂的方法以及通过检测方法筛选抗血小板剂的方法。
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公开(公告)号：US20050159355A1
公开(公告)日：2005-07-21
申请号：US11012199
申请日：2004-12-16
IPC分类号： A61K38/00 , C07K14/705 , C07K14/72 , C12N1/19 , C12N1/21 , C12N15/12 , A61K38/17 , C07H21/04 , C12Q1/68
CPC分类号： C07K14/723 , A61K38/00 , C07K14/705
摘要： This invention provides a nucleic acid encoding a novel leukotriene B4 receptor, a vector containing this nucleic acid, a method for screening a substance capable of modifying the activity of the receptor by using a host cell containing the vector and the receptor, and pharmaceutical compositions for inflammatory diseases containing as the active ingredient a substance capable of modifying the activity of the receptor.
摘要翻译： 本发明提供编码新型白三烯B4受体的核酸，含有该核酸的载体，通过使用含有载体和受体的宿主细胞筛选能够改变受体活性的物质的方法，以及用于 含有能够改变受体活性的物质作为活性成分的炎性疾病。
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公开(公告)号：US07217800B2
公开(公告)日：2007-05-15
申请号：US10932004
申请日：2004-09-02
申请人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
发明人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
IPC分类号： C07K16/28
CPC分类号： C07K14/705 , A61K38/00 , G01N33/6893 , G01N2500/00 , G01N2500/04
摘要： A cDNA encoding a novel LTC4 receptor has been isolated. Provision of the novel protein, an LTC4 receptor, enabled binding experiments using the LTC4. By screening for compounds that modulate LTC4 receptor activity based on these binding experiments, development of drugs targeting the LTC4 receptor becomes possible.
摘要翻译： 已经分离了编码新型LTC4受体的cDNA。 提供新的蛋白质，LTC4受体，能够使用LTC4N4进行结合实验。 通过基于这些结合实验筛选调节LTC4受体活性的化合物，开发靶向LTC4受体的药物成为可能。
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公开(公告)号：US06878525B1
公开(公告)日：2005-04-12
申请号：US09979603
申请日：2000-09-13
申请人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
发明人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
IPC分类号： A61K38/00 , A61P9/08 , A61P43/00 , C07K14/705 , C12N15/12 , G01N33/68 , G01N33/567
CPC分类号： C07K14/705 , A61K38/00 , G01N33/6893 , G01N2500/00 , G01N2500/04
摘要： A cDNA encoding a novel LTC4 receptor has been isolated. Provision of the novel protein, an LTC4 receptor, enabled binding experiments using the LTC4. By screening for compounds that modulate LTC4 receptor activity based on these binding experiments, development of drugs targeting the LTC4 receptor becomes possible.
摘要翻译： 已经分离出编码新的LTC4受体的cDNA。 提供新的蛋白质LTC4受体，启用使用LTC4的结合实验。 通过筛选基于这些结合实验调节LTC4受体活性的化合物，开发靶向LTC4受体的药物成为可能。
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公开(公告)号：US07341849B2
公开(公告)日：2008-03-11
申请号：US10898329
申请日：2004-07-26
申请人： Mitsuyuki Matsumoto , Toru Sugimoto , Jun Takasaki , Masazumi Kamohara , Tetsu Saito , Masato Kobayashi
发明人： Mitsuyuki Matsumoto , Toru Sugimoto , Jun Takasaki , Masazumi Kamohara , Tetsu Saito , Masato Kobayashi
CPC分类号： C07K16/28 , A61K38/00 , C07K14/705 , C07K14/723
摘要： This invention belongs to the genetic engineering field, and provides novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system, genes coding for these proteins, screening methods using these proteins and so on. As one of the methods for obtaining the G protein-coupled receptor proteins of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.
摘要翻译： 本发明属于遗传工程领域，提供了在中枢神经系统中表达的新型G蛋白偶联受体家族蛋白SREB1，SREB2和SREB3，编码这些蛋白质的基因，使用这些蛋白质的筛选方法等。 作为获得本发明的G蛋白偶联受体蛋白质的方法之一，使用从人或大鼠脑组织或脑源性细胞提取的mRNA作为模板，使用插入整个部分的两个引物进行RT-PCR 或G蛋白偶联受体蛋白质翻译区的一部分，从而获得对应于G蛋白偶联受体蛋白或其一部分的cDNA，并将cDNA整合到合适的表达载体中并在宿主细胞中表达。
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公开(公告)号：US20050037968A1
公开(公告)日：2005-02-17
申请号：US10932004
申请日：2004-09-02
申请人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
发明人： Jun Takasaki , Masazumi Kamohara , Mitsuyuki Matsumoto , Tetsu Saito , Tohru Sugimoto , Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Yuri Kawai
IPC分类号： A61K38/00 , A61P9/08 , A61P43/00 , C07K14/705 , C12N15/12 , G01N33/68 , G01N33/53 , A61K38/17
CPC分类号： C07K14/705 , A61K38/00 , G01N33/6893 , G01N2500/00 , G01N2500/04
摘要： A cDNA encoding a novel LTC4 receptor has been isolated. Provision of the novel protein, an LTC4 receptor, enabled binding experiments using the LTC4. By screening for compounds that modulate LTC4 receptor activity based on these binding experiments, development of drugs targeting the LTC4 receptor becomes possible.
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公开(公告)号：US06555344B1
公开(公告)日：2003-04-29
申请号：US09622439
申请日：2000-08-17
申请人： Mitsuyuki Matsumoto , Toru Sugimoto , Jun Takasaki , Masazumi Kamohara , Tetsu Saito , Masato Kobayashi
发明人： Mitsuyuki Matsumoto , Toru Sugimoto , Jun Takasaki , Masazumi Kamohara , Tetsu Saito , Masato Kobayashi
IPC分类号： C12P2300
CPC分类号： C07K16/28 , A61K38/00 , C07K14/705 , C07K14/723
摘要： This invention belongs to the genetic engineering field, and provides the novel G protein-coupled receptor family protein SREB2 expressed in the central nervous system, genes coding for the protein, screening methods using the protein and so on. As one of the methods for obtaining the G protein-coupled receptor protein of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.
摘要翻译： 本发明属于遗传工程领域，提供了在中枢神经系统中表达的新型G蛋白偶联受体家族蛋白SREB2，编码蛋白质的基因，使用蛋白质的筛选方法等。 作为获得本发明的G蛋白偶联受体蛋白质的方法之一，使用从人或大鼠脑组织或脑源性细胞提取的mRNA作为模板并使用插入整个部分的两个引物进行RT-PCR 或G蛋白偶联受体蛋白质翻译区的一部分，从而获得对应于G蛋白偶联受体蛋白或其一部分的cDNA，并将cDNA整合到合适的表达载体中并在宿主细胞中表达。
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