利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.031 秒)

    Optical fiber based fluorescent immunoassay apparatus
    2.
    发明授权
    Optical fiber based fluorescent immunoassay apparatus 失效
    基于光纤的荧光免疫测定仪

    公开(公告)号：US5449625A

    公开(公告)日：1995-09-12

    申请号：US98394

    申请日：1993-08-16

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Yasunori Sakai ,  Ken-ichi Shimada

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Yasunori Sakai ,  Ken-ichi Shimada

    IPC分类号： G01N21/64 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

    CPC分类号： G01N33/54366 ,  G01N33/5302 ,  G01N33/582 ,  Y10S435/968 ,  Y10S435/969 ,  Y10S436/80 ,  Y10S436/807

    摘要： The present invention relates to an apparatus for performing a fluoroimmunoassay, the apparatus comprising an excitation light source, a fluorescence detector, an optical fiber for an excitation light from the excitation light source to which an antigen or antibody is bound, and a spectroscope, in which the mentioned antigen or antibody is bound to an outgoing end surface of the optical fiber for the excitation light. In the apparatus the spectroscope is constructed so that it introduces the excitation light emitted from the excitation light source to a incident end surface of the optical fiber for the excitation light and introduces a fluorescence emitted from a fluorescence-labeled antibody or antigen which forms an immunological complex with the antigen or antibody to the fluorescence detector. In the apparatus the outgoing end surface of the optical fiber for the excitation light has a transmittance for the excitation light from the excitation light source. The invention also relates to a sensing chip which is used in the apparatus for performing a fluoroimmunoassay therewith.

    摘要翻译： PCT No.PCT / JP92 / 01652 Sec。 371日期：1993年8月16日 102（e）日期1993年8月16日PCT提交1992年12月17日PCT公布。 公开号WO93 / 13418 日期：1993年7月8日。本发明涉及一种用于进行荧光免疫测定的装置，该装置包括激发光源，荧光检测器，用于来自激发光源的激发光的光纤，抗原或抗体 所述抗原或抗体结合到所述光纤的出射端表面用于激发光。 在该装置中，分光镜被构造成使得从激发光源发射的激发光引入用于激发光的光纤的入射端面，并引入从形成免疫学的荧光标记的抗体或抗原发射的荧光 与荧光检测器的抗原或抗体复合。 在该装置中，用于激发光的光纤的出射端面对于来自激发光源的激发光具有透射率。 本发明还涉及用于与其进行荧光免疫测定的装置中的感测芯片。

    Method for immobilizing biocatalyst in granular form with a photo-crosslinkable resin
    3.
    发明授权
    Method for immobilizing biocatalyst in granular form with a photo-crosslinkable resin 失效
    用光交联树脂固定颗粒状生物催化剂的方法

    公开(公告)号：US5268286A

    公开(公告)日：1993-12-07

    申请号：US665360

    申请日：1991-03-06

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Masamichi Kamihira ,  Mikiko Hata ,  Takamitsu Iida ,  Masahiro Sakamoto ,  Hiroshi Itoh ,  Naoki Isobe

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Masamichi Kamihira ,  Mikiko Hata ,  Takamitsu Iida ,  Masahiro Sakamoto ,  Hiroshi Itoh ,  Naoki Isobe

    IPC分类号： C12N11/08 ,  C12N11/10 ,  C12N11/04 ,  C12N11/12

    CPC分类号： C12N11/10 ,  C12N11/08

    摘要： A biocatalyst is immobilized with a graft product of a polymer and saponified polyvinyl acetate containing a stilbazolium group as a photo-crosslinking group. The polymer is preferably gelatin, collagen, starch, cellulose, gum arabic, tragacanth gum, carrageenan, mannan, dextrin or alginic acid. The graft product is prepared by bonding the polymer to the polyvinyl acetate, either directly through functional groups of the polymer and polyvinyl acetate or through a crosslinking agent. The polymer provides affinity for the biocatalyst and by grafting the polymer to the saponified polyvinyl acetate, damage to cells upon immobilization is reduced. A liquid composition containing a biocatalyst and the graft product is added to an aqueous solution of an inorganic salt or an organic salt to form a granular gel and the gel is irradiated with actinic rays to cure the gel.

    摘要翻译： 生物催化剂用聚合物的接枝产物和含有咔唑基作为光交联基团的皂化聚乙酸乙烯酯固定化。 聚合物优选为明胶，胶原，淀粉，纤维素，阿拉伯胶，黄蓍胶，角叉菜胶，甘露聚糖，糊精或海藻酸。 通过将聚合物直接通过聚合物和聚乙酸乙烯酯的官能团或通过交联剂直接结合聚乙酸乙烯酯来制备接枝产物。 聚合物对生物催化剂提供亲和性，并且通过将聚合物接枝到皂化的聚乙酸乙烯酯上，固定时对细胞的损伤降低。 将含有生物催化剂和接枝产物的液体组合物加入到无机盐或有机盐的水溶液中以形成颗粒状凝胶，并用光化射线照射凝胶以固化凝胶。

    Method for producing optical fiber having formyl groups on core surface thereof
    4.
    发明授权
    Method for producing optical fiber having formyl groups on core surface thereof 失效
    在其芯表面上具有甲酰基的光纤的制造方法

    公开(公告)号：US5354574A

    公开(公告)日：1994-10-11

    申请号：US80615

    申请日：1993-06-22

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Ken-ichi Shimada ,  Kazue Ohe ,  Yasunori Sakai

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Ken-ichi Shimada ,  Kazue Ohe ,  Yasunori Sakai

    IPC分类号： G01N33/545 ,  G01N33/547 ,  B05D5/06

    CPC分类号： G01N33/547 ,  G01N33/545

    摘要： The present invention is directed to a method for producing an optical fiber having formyl groups on a core surface. In one embodiment, a plastic optical fiber having formyl groups on a core surface is obtained by immersing the core surface of the plastic optical fiber in a treating solution containing at least a 0.5 to 40 mM alkali metal hydroxide and a compound having formyl groups in water or lower alcohol solvent. In the other embodiment, a plastic optical fiber having formyl groups on a core surface is obtained by introducing a vic-diol group, a primary hydroxyl group or an alkenyl group on the core surface, and then oxidizing it. Turbidity on the core surface of the optical fiber thus obtained is minimized, thereby reducing the transmittance losses of light.

    摘要翻译： 本发明涉及一种在芯表面上制备具有甲酰基的光纤的方法。 在一个实施方案中，通过将塑料光纤的芯表面浸渍在含有至少0.5至40mM碱金属氢氧化物和在水中具有甲酰基的化合物的处理溶液中来获得在芯表面上具有甲酰基的塑料光纤 或低级醇溶剂。 在另一实施方式中，通过在芯表面上引入邻二醇基，伯羟基或烯基，然后将其氧化来获得在芯表面上具有甲酰基的塑料光纤。 由此获得的光纤的芯表面上的浊度最小化，从而降低光的透射率损失。

    Transgenic Bird And Method Of Constructing The Same
    5.
    发明申请
    Transgenic Bird And Method Of Constructing The Same 审中-公开
    转基因鸟及其构建方法

    公开(公告)号：US20070214511A1

    公开(公告)日：2007-09-13

    申请号：US10585693

    申请日：2004-11-05

    申请人： Takashi Yamashita ,  Takuya Shindo ,  Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    发明人： Takashi Yamashita ,  Takuya Shindo ,  Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    IPC分类号： A01K67/027 ,  C12N15/89 ,  C12N5/16 ,  C12P21/00 ,  C12Q1/68

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01

    摘要： The present invention provides a G1 transgenic bird or an offspring thereof by: incubating a fertilized avian egg, microinjecting, into the early embryo thereof at a stage except for and after the blastodermic stage just after egg laying, a replication-deficient retroviral vector coding for a useful protein, allowing the egg to hatch out to thereby obtain a G0 transgenic chimeric bird, and mating the G0 transgenic chimeric bird with another G0 transgenic chimeric bird or an offspring thereof or with a wild-type bird. The present invention is a G1 transgenic bird or an offspring thereof by: incubating a fertilized avian egg, microinjecting, into the early embryo thereof at a stage except for and after the blastodermic stage just after egg laying, a replication-deficient retroviral vector coding for a useful protein, allowing the egg to hatch out to thereby obtain a G0 transgenic chimeric bird, and mating the G0 transgenic chimeric bird with another G0 transgenic chimeric bird or an offspring thereof or with a wild-type bird.

    摘要翻译： 本发明通过以下方式提供G1转基因鸟或其后代：在产卵后的胚根期以外的阶段将受精禽卵孵育至其早期胚胎，将其编码为复制缺陷型逆转录病毒载体 一种有用的蛋白质，允许卵孵出，从而获得G0转基因嵌合鸟，并将G0转基因嵌合鸟与另一种G0转基因嵌合鸟或其后代或野生型鸟交配。 本发明是一种G1转基因鸟或其后代，其特征在于：在产卵后的胚根期以外的阶段将受精禽卵孵化至其早期胚胎，将其编码为复制缺陷型逆转录病毒载体 一种有用的蛋白质，允许卵孵出，从而获得G0转基因嵌合鸟，并将G0转基因嵌合鸟与另一种G0转基因嵌合鸟或其后代或野生型鸟交配。

    &bgr;1,3-galactosyltransferase and DNA encoding the same
    6.
    发明授权
    &bgr;1,3-galactosyltransferase and DNA encoding the same 失效
    β1,3-半乳糖基转移酶和编码相同的DNA

    公开(公告)号：US06828128B2

    公开(公告)日：2004-12-07

    申请号：US09900038

    申请日：2001-07-09

    申请人： Katsuhide Miyake ,  Masaki Watanabe ,  Shinji Iijima

    发明人： Katsuhide Miyake ,  Masaki Watanabe ,  Shinji Iijima

    IPC分类号： C12P1918

    CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12Y204/01062

    摘要： The present invention provides a protein having a &bgr;1,3-galactosyltransferase activity derived from a microorganism; a DNA encoding the protein; a recombinant DNA containing the DNA and a vector; a transformant obtained by introducing the recombinant DNA into a host cell; a method for producing a protein having a &bgr;1,3-galactosyltransferase activity using the transformant; and a method for producing a galactose-containing carbohydrate using the transformant.

    摘要翻译： 本发明提供一种具有衍生自微生物的β1,3-半乳糖基转移酶活性的蛋白质; 编码蛋白质的DNA; 含有DNA和载体的重组DNA; 通过将重组DNA导入宿主细胞获得的转化体; 使用该转化体生产具有β1,3-半乳糖基转移酶活性的蛋白质的方法; 以及使用该转化体生产含半乳糖的碳水化合物的方法。

    Method of constructing transgenic bird using lentivirus vector and transgenic bird obtained thereby
    7.
    发明申请
    Method of constructing transgenic bird using lentivirus vector and transgenic bird obtained thereby 审中-公开
    使用慢病毒载体和由此获得的转基因鸟构建转基因鸟的方法

    公开(公告)号：US20060259997A1

    公开(公告)日：2006-11-16

    申请号：US10569268

    申请日：2004-08-27

    申请人： Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    发明人： Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    IPC分类号： A01K67/027 ,  C12N5/06 ,  C12N15/867

    CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A01K2227/30 ,  C12N15/86 ,  C12N2740/15043 ,  C12N2740/15045

    摘要： The present invention provides a lentivirus vector which comprises a coat protein containing VSV-G; a recombinant bird host which is constructed by infecting a bird host with the lentivirus vector; a method of constructing the same; a transgenic chimeric bird which is constructed by infection with the lentivirus vector and offspring thereof. The present invention also provides a method of constructing a G0 transgenic chimeric bird and offspring thereof which comprises infecting fertilized bird egg early embryos with a lentivirus vector comprising a coat protein containing VSV-G, and incubating the embryos; and a G0 transgenic chimeric bird and offspring thereof which is constructed by the method mentioned above. The present invention provides a transgenic bird which is constructed by infecting a bird early embryos with a lentivirus vector comprising a coat protein pseudotyped with VSV-G.

    摘要翻译： 本发明提供一种慢病毒载体，其包含含有VSV-G的外壳蛋白; 通过用慢病毒载体感染鸟类宿主构建的重组禽类宿主; 一种构建它的方法; 通过感染慢病毒载体及其后代构建的转基因嵌合鸟。 本发明还提供了构建G0转基因嵌合鸟及其后代的方法，其包括用含有VSV-G的外壳蛋白的慢病毒载体感染受精鸟卵早期胚胎，并培养胚胎; 和通过上述方法构建的G0转基因嵌合鸟及其后代。 本发明提供一种转基因鸟，其通过用包含用VSV-G假型包被的蛋白质的慢病毒载体感染鸟类早期胚胎而构建。

    β1,3-galactosyltransferase and DNA encoding the same
    10.
    发明授权
    β1,3-galactosyltransferase and DNA encoding the same 失效
    β1,3-半乳糖基转移酶和编码相同的DNA

    公开(公告)号：US07238100B2

    公开(公告)日：2007-07-03

    申请号：US10865873

    申请日：2004-06-14

    申请人： Katsuhide Miyake ,  Masaki Watanabe ,  Shinji Iijima

    发明人： Katsuhide Miyake ,  Masaki Watanabe ,  Shinji Iijima

    IPC分类号： C07H21/04 ,  C12P9/18 ,  C12N9/10 ,  C12N1/21 ,  C12N15/63

    CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12Y204/01062

    摘要： The present invention provides a protein having a β1,3-galactosyltransferase activity derived from a microorganism; a DNA encoding the protein; a recombinant DNA containing the DNA and a vector; a transformant obtained by introducing the recombinant DNA into a host cell; a method for producing a protein having a β1,3-galactosyltransferase activity using the transformant; and a method for producing a galactose-containing carbohydrate using the transformant.

    摘要翻译： 本发明提供一种具有衍生自微生物的β1,3-半乳糖基转移酶活性的蛋白质; 编码蛋白质的DNA; 含有DNA和载体的重组DNA; 通过将重组DNA导入宿主细胞获得的转化体; 使用该转化体生产具有β1,3-半乳糖基转移酶活性的蛋白质的方法; 以及使用该转化体生产含半乳糖的碳水化合物的方法。