公开(公告)号：US20080106330A1

公开(公告)日：2008-05-08

申请号：US11896899

申请日：2007-09-06

申请人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

发明人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

IPC分类号： H03F3/38

CPC分类号： H03F3/387 ,  H03F3/45475 ,  H03F3/45977 ,  H03F2200/261 ,  H03F2203/45136 ,  H03F2203/45212

摘要： In a feedback amplifier circuit, a first switching device executes an auto-zero operation by inputting a signal outputted from an amplifier to an input terminal of the amplifier during an auto-zero operation interval prior to an amplification interval. A first capacitor accumulates and holds an offset voltage at the output terminal of the amplifier during the auto-zero operation interval, and cancels an offset voltage at the input terminal of the amplifier by an accumulated and held offset voltage during an amplification interval subsequent to the auto-zero operation interval. A second switching device grounds the feedback point of the feedback circuit during the auto-zero operation interval. A second capacitor blocks a DC voltage, and accumulates and holds an offset voltage at an output terminal of the amplifier, and cancels an offset voltage at an input terminal of the amplifier by the accumulated and held offset voltage during an amplification interval.

摘要翻译： 在反馈放大器电路中，第一开关器件在放大间隔之前的自动归零操作间隔期间，通过将从放大器输出的信号输入到放大器的输入端子来执行自动归零操作。 第一电容器在自动归零操作间隔期间在放大器的输出端积累并保持偏移电压，并且在放大器的输出端之后的放大间隔期间通过累积和保持的偏移电压来消除放大器的输入端的偏移电压 自动归零运行间隔。 第二开关装置在自动归零操作间隔期间将反馈电路的反馈点接地。 第二电容器阻塞DC电压，并且在放大器的输出端累积并保持偏移电压，并且在放大间隔期间通过累积和保持的偏移电压来消除放大器输入端的偏移电压。

公开(公告)号：US07907076B2

公开(公告)日：2011-03-15

申请号：US12582305

申请日：2009-10-20

申请人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

发明人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

IPC分类号： H03M1/34

CPC分类号： H03F3/45475 ,  H03F3/387 ,  H03F2203/45512 ,  H03M1/0663 ,  H03M1/12

摘要： A differential amplifier circuit is provided with an operational amplifier and a modulator. The operational amplifier includes a feedback capacitance, and amplifies an analog input signal and outputs an amplified analog output signal. The modulator is connected to a virtual ground point of an input terminal of the operational amplifier, and the modulator switches between a pair of inputted analog differential signals to alternately select one of the analog differential signals based on a predetermined modulation control signal, and outputs a selected analog differential signal. The differential amplifier circuit alternately folds and amplifies the analog input signal within a predetermined input level limit range to generate a signal having different polarities sequentially so as to start from a voltage potential of the virtual ground point at a timing of the modulation control signal. In addition, an converter apparatus is provided with the differential amplifier circuit.

摘要翻译： 差分放大器电路具有运算放大器和调制器。 运算放大器包括反馈电容，并放大模拟输入信号并输出​​放大的模拟输出信号。 调制器连接到运算放大器的输入端的虚拟接地点，并且调制器在一对输入的模拟差分信号之间切换，以基于预定的调制控制信号交替地选择一个模拟差分信号，并输出 选择模拟差分信号。 差分放大器电路在预定输入电平限制范围内交替地折叠和放大模拟输入信号，以在调制控制信号的定时从顺序地产生具有不同极性的信号，以便从虚拟接地点的电压电位开始。 另外，转换器装置设置有差动放大电路。

公开(公告)号：US07589587B2

公开(公告)日：2009-09-15

申请号：US11896899

申请日：2007-09-06

申请人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

发明人： Takeshi Yoshida ,  Yoshihiro Masui ,  Atsushi Iwata ,  Kunihiko Gotoh

IPC分类号： H03F1/02

CPC分类号： H03F3/387 ,  H03F3/45475 ,  H03F3/45977 ,  H03F2200/261 ,  H03F2203/45136 ,  H03F2203/45212

摘要： In a feedback amplifier circuit, a first switching device executes an auto-zero operation by inputting a signal outputted from an amplifier to an input terminal of the amplifier during an auto-zero operation interval prior to an amplification interval. A first capacitor accumulates and holds an offset voltage at the output terminal of the amplifier during the auto-zero operation interval, and cancels an offset voltage at the input terminal of the amplifier by an accumulated and held offset voltage during an amplification interval subsequent to the auto-zero operation interval. A second switching device grounds the feedback point of the feedback circuit during the auto-zero operation interval. A second capacitor blocks a DC voltage, and accumulates and holds an offset voltage at an output terminal of the amplifier, and cancels an offset voltage at an input terminal of the amplifier by the accumulated and held offset voltage during an amplification interval.

摘要翻译： 在反馈放大器电路中，第一开关器件在放大间隔之前的自动归零操作间隔期间，通过将从放大器输出的信号输入到放大器的输入端子来执行自动归零操作。 第一电容器在自动归零操作间隔期间在放大器的输出端积累并保持偏移电压，并且在放大器的输出端之后的放大间隔期间通过累积和保持的偏移电压来消除放大器的输入端的偏移电压 自动归零运行间隔。 第二开关装置在自动归零操作间隔期间将反馈电路的反馈点接地。 第二电容器阻塞DC电压，并且在放大器的输出端累积并保持偏移电压，并且在放大间隔期间通过累积和保持的偏移电压来消除放大器输入端的偏移电压。

公开(公告)号：US20060244521A1

公开(公告)日：2006-11-02

申请号：US11390116

申请日：2006-03-28

申请人： Takeshi Yoshida ,  Atsushi Iwata ,  Mamoru Sasaki ,  Takayuki Mashimo ,  Yoshihiro Masui ,  Junji Nakatsuka

发明人： Takeshi Yoshida ,  Atsushi Iwata ,  Mamoru Sasaki ,  Takayuki Mashimo ,  Yoshihiro Masui ,  Junji Nakatsuka

IPC分类号： H03F1/02

CPC分类号： H03F3/45775 ,  H03F3/393

摘要： In a chopper amplifier circuit operable at a low voltage utilizing a switched operational amplifier, a chopper modulator chopper-modulates an input signal according to a predetermined control signal, and outputs a chopper-modulated signal. An amplifier circuit constituted by the switched operational amplifier amplifies the chopper-modulated signal outputted from the chopper modulator, and outputs an amplified chopper-modulated signal. A chopper-demodulator of the switched operational amplifier chopper-demodulates the amplified chopper-modulated signal outputted from the amplifier circuit according to the control signal, and outputs a demodulated output signal as a chopper-amplified output signal from an output terminal. A chopper modulator chopper-modulates a demodulated signal outputted from the chopper demodulator according to the control signal, and outputs a chopper-modulated signal to an input terminal of the amplifier circuit.

摘要翻译： 在利用开关式运算放大器在低电压工作的斩波放大器电路中，斩波调制器根据预定的控制信号对输入信号进行斩波调制，并输出斩波调制信号。 由开关运算放大器构成的放大电路放大从斩波调制器输出的斩波调制信号，并输出放大的斩波调制信号。 切换运算放大器的斩波解调器根据控制信号对从放大器电路输出的放大的斩波调制信号进行斩波，并从输出端子输出作为斩波放大输出信号的解调输出信号。 斩波调制器根据控制信号对从斩波解调器输出的解调信号进行斩波调制，并将斩波调制信号输出到放大电路的输入端。

公开(公告)号：US07336123B2

公开(公告)日：2008-02-26

申请号：US11390116

申请日：2006-03-28

申请人： Takeshi Yoshida ,  Atsushi Iwata ,  Mamoru Sasaki ,  Takayuki Mashimo ,  Yoshihiro Masui ,  Junji Nakatsuka

发明人： Takeshi Yoshida ,  Atsushi Iwata ,  Mamoru Sasaki ,  Takayuki Mashimo ,  Yoshihiro Masui ,  Junji Nakatsuka

IPC分类号： H03F1/02

CPC分类号： H03F3/45775 ,  H03F3/393

摘要： In a chopper amplifier circuit operable at a low voltage utilizing a switched operational amplifier, a chopper modulator chopper-modulates an input signal according to a predetermined control signal, and outputs a chopper-modulated signal. An amplifier circuit constituted by the switched operational amplifier amplifies the chopper-modulated signal outputted from the chopper modulator, and outputs an amplified chopper-modulated signal. A chopper-demodulator of the switched operational amplifier chopper-demodulates the amplified chopper-modulated signal outputted from the amplifier circuit according to the control signal, and outputs a demodulated output signal as a chopper-amplified output signal from an output terminal. A chopper modulator chopper-modulates a demodulated signal outputted from the chopper demodulator according to the control signal, and outputs a chopper-modulated signal to an input terminal of the amplifier circuit.

摘要翻译： 在利用开关式运算放大器在低电压工作的斩波放大器电路中，斩波调制器根据预定的控制信号对输入信号进行斩波调制，并输出斩波调制信号。 由开关运算放大器构成的放大电路放大从斩波调制器输出的斩波调制信号，并输出放大的斩波调制信号。 切换运算放大器的斩波解调器根据控制信号对从放大器电路输出的放大的斩波调制信号进行斩波，并从输出端子输出作为斩波放大输出信号的解调输出信号。 斩波调制器根据控制信号对从斩波解调器输出的解调信号进行斩波调制，并将斩波调制信号输出到放大电路的输入端。

公开(公告)号：US4757013A

公开(公告)日：1988-07-12

申请号：US880358

申请日：1986-06-26

申请人： Masayori Inouye ,  Yoshihiro Masui

发明人： Masayori Inouye ,  Yoshihiro Masui

IPC分类号： C12N15/62 ,  C12N15/70 ,  C12P21/00 ,  C12N1/20 ,  C12N15/00 ,  C12P21/02

CPC分类号： C12N15/70 ,  C12N15/62 ,  Y10S930/12

摘要： Methods and compositions are provided for regulated expression and secretion of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with four functional fragments derived from the lipoprotein gene of E. coli. The plasmids also include a DNA fragment coding for the signal peptide of the ompA protein of E. coli, positioned such that the desired polypeptide is expressed with the ompA signal peptide at its amino terminus, thereby allowing efficient secretion across the cytoplasmic membrane. The plasmids further include a DNA sequence coding for a specific segment of the E. coli lac promoter-operator, which is positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate functional E. coli lacI gene coding for the associated repressor molecule which can interact with the lac promoter-operator to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the lipoprotein promoter and the lac promoter-operator, although transcription from either promoter is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoters. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of normally secreted proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.

摘要翻译： 提供了用于调节转化细菌宿主中多肽表达和分泌的方法和组合物。 一类新颖的质粒克隆载体包括编码与阅读阶段转录表达连接的期望多肽（或其插入位点）的DNA序列，其中四个功能片段衍生自大肠杆菌的脂蛋白基因。 质粒还包括编码大肠杆菌的ompA蛋白的信号肽的DNA片段，其定位使得所需的多肽在其氨基末端用ompA信号肽表达，从而允许跨细胞质膜的有效分泌。 质粒还包括编码大肠杆菌lac启动子 - 操纵子的特定区段的DNA序列，其定位于所需多肽的转录表达的正确取向，以及编码所述多肽的单独的功能性大肠杆菌lacI基因 可与lac启动子 - 操纵子相互作用以阻止转录的相关阻遏物分子。 所需多肽的表达在脂蛋白启动子和lac启动子 - 操纵子的控制之下，尽管来自任一启动子的转录通常被阻抑物分子阻断。 然而，阻遏物可以通过诱导剂分子选择性地失活以允许来自两个启动子的所需多肽的转录表达。 所述方法利用这种质粒将遗传能力引入微生物以产生正常分泌的蛋白质，例如医学或商业上有用的激素，酶，免疫原性蛋白质或其中间体，但仅在适当的诱导剂存在下。

公开(公告)号：US4863855A

公开(公告)日：1989-09-05

申请号：US378481

申请日：1982-05-14

申请人： Masayori Inouye ,  Kenzo Nakamura ,  Yoshihiro Masui

发明人： Masayori Inouye ,  Kenzo Nakamura ,  Yoshihiro Masui

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/62 ,  C12N15/70 ,  C12P21/00

CPC分类号： C12N15/62 ,  C12N15/70

摘要： Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with one or more functional fragments derived from an outer membrane protein gene of a Gram-negative bacterium. The plasmids also include an inducible promoter sequence positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate DNA sequence coding for a repressor molecule which can interact with the inducible promoter to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promoter is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoters. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.

摘要翻译： 提供了用于在转化的细菌宿主中调节多肽表达的方法和组合物。 一类新颖的质粒克隆载体包括编码所需多肽（或其插入位点）的DNA序列，其与阅读阶段的转录表达连接，其与来自革兰氏阴性细菌的外膜蛋白基因的一种或多种功能片段连接。 质粒还包括位于适当取向的诱导型启动子序列，用于所需多肽的转录表达，以及编码可与诱导型启动子相互作用以阻止其转录的阻遏物分子的单独DNA序列。 所需多肽的表达受组成型启动子和诱导型启动子的控制，尽管来自任一启动子的转录通常被阻遏物分子阻断。 然而，阻遏物可以通过诱导剂分子选择性地失活以允许来自两个启动子的所需多肽的转录表达。 所述方法利用这种质粒将遗传能力引入微生物以产生蛋白质，例如医学或商业上有用的激素，酶，免疫原性蛋白质或其中间体，但仅在适当的诱导剂存在下。

公开(公告)号：US5723117A

公开(公告)日：1998-03-03

申请号：US139862

申请日：1993-10-22

申请人： Satoru Nakai ,  Seiji Akamatsu ,  Yoshihiro Masui ,  Tsutomu Nishida ,  Takashi Kamogashira ,  Yoshikatu Hirai

发明人： Satoru Nakai ,  Seiji Akamatsu ,  Yoshihiro Masui ,  Tsutomu Nishida ,  Takashi Kamogashira ,  Yoshikatu Hirai

IPC分类号： A61K38/00 ,  C07K14/545 ,  A61K38/20

CPC分类号： C07K14/545 ,  A61K38/00

摘要： A drug for preventing and curing liver diseases comprising at least one member selected from interleukin-1 and its derivatives as an effective component. The drug meaningfully suppresses GOT and GPT activities in blood which are markers of liver diseases, and also suppresses symptoms such as choloplania.

摘要翻译： 一种预防和治疗肝病的药物，其包含选自白介素-1及其衍生物中的至少一种作为有效成分。 该药有意义地抑制作为肝脏疾病标志物的血液中的GOT和GPT活性，并抑制诸如胆红素等症状。

公开(公告)号：US5120534A

公开(公告)日：1992-06-09

申请号：US386073

申请日：1989-07-28

申请人： Yoshikatsu Hirai ,  Satoru Nakai ,  Koutoku Aihara ,  Kazuyoshi Kawai ,  Mayumi Kaneta ,  Takashi Kamogashira ,  Yoshihiro Masui

发明人： Yoshikatsu Hirai ,  Satoru Nakai ,  Koutoku Aihara ,  Kazuyoshi Kawai ,  Mayumi Kaneta ,  Takashi Kamogashira ,  Yoshihiro Masui

IPC分类号： A61K38/00 ,  C07K14/545

CPC分类号： C07K14/545 ,  A61K38/00

摘要： The present invention provides a medicament for treating thrombocytopenia comprising as an active component at least one polypeptide selected from IL-1 and derivative thereof and a IL-1.alpha. derivative having an amino acid sequence of the formula (.alpha.) which is so modified as to fulfill at least one of the requirements of: deletion of the 16-position Arg; replacement of the 16-position Arg by another amino acid residue; deletion of the sequence of the 1-position Ser to the 14-position Phe and deletion of the amino acid sequence of the 1-position Ser to the 15-position Met.

公开(公告)号：US5756675A

公开(公告)日：1998-05-26

申请号：US254419

申请日：1994-06-06

申请人： Yoshikatsu Hirai ,  Satoru Nakai ,  Koutoku Aihara ,  Kazuyoshi Kawai ,  Mayumi Kaneta ,  Takashi Kamogashira ,  Yoshihiro Masui

发明人： Yoshikatsu Hirai ,  Satoru Nakai ,  Koutoku Aihara ,  Kazuyoshi Kawai ,  Mayumi Kaneta ,  Takashi Kamogashira ,  Yoshihiro Masui

IPC分类号： A61K38/00 ,  C07K14/545 ,  C07K14/54

CPC分类号： C07K14/545 ,  A61K38/00

摘要： The present invention provides a medicament for treating thrombocytopenia comprising as an active component at least one polypeptide selected from IL-1 and derivative thereof and a Il-1.alpha. derivative having an amino acid sequence of the formula (.alpha.) which is so modified as to fulfill at least one of the requirements of: deletion of the 16-position Arg; replacement of the 16-position Arg by another amino acid residue; deletion of the sequence of the 1-position Ser to the 14-position Phe and deletion of the amino acid sequence of the 1-position Ser to the 15-position Met.

摘要翻译： 本发明提供了治疗血小板减少症的药物，其包含至少一种选自IL-1及其衍生物的多肽作为活性成分和具有式（α）的氨基酸序列的II-1α衍生物，其被修饰为 满足至少一项要求：删除16位Arg; 用另一个氨基酸残基取代16位Arg; 将1位Ser序列删除至14位Phe，并将1位Ser的氨基酸序列缺失至15位Met。