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    Method to increase detection efficiency of real time PCR microarray by quartz material
    3.
    发明授权
    Method to increase detection efficiency of real time PCR microarray by quartz material 有权
    通过石英材料提高实时PCR微阵列检测效率的方法

    公开(公告)号：US09539571B2

    公开(公告)日：2017-01-10

    申请号：US13522938

    申请日：2010-01-20

    Applicant: Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    Inventor： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00

    CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    Abstract: A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    Abstract translation: 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    Microarray reader based on evanescent wave detection
    4.
    发明授权
    Microarray reader based on evanescent wave detection 有权
    基于ev逝波检测的微阵列阅读器

    公开(公告)号：US08809810B2

    公开(公告)日：2014-08-19

    申请号：US13697453

    申请日：2010-05-20

    Applicant: Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    Inventor： Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    IPC: G01N21/64

    CPC classification number: G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    Abstract: The disclosure relates to a microarray reader that includes a light source which emits light and beam shaping elements positioned near the light source to direct the light. The microarray reader further includes a microarray that is at least formed of an optical substrate and a reaction chamber in contact with the optical substrate. A buffer solution is encapsulated by the optical substrate and the reaction chamber and a holder aligns the microarray relative to the light source such that when light hits the buffer solution molecules in the buffer solution are excited to emit fluorescent light. A temperature control component is thermally engaged with the holder such that the temperature control component adjusts the temperature of the buffer solution. The microarray reader further includes a sensor that receives the fluorescent light emitted by the excited molecules in the buffer solution to generate a signal.

    Abstract translation: 本发明涉及一种微阵列读取器，其包括发射光的光源和位于光源附近的光束成形元件以引导光。 微阵列读取器还包括至少由光学基底和与光学基底接触的反应室形成的微阵列。 缓冲溶液被光学基底和反应室封装，并且保持器将微阵列相对于光源对准，使得当光照射缓冲溶液时，激发缓冲溶液中的分子发射荧光。 温度控制部件与保持器热接合，使得温度控制部件调节缓冲溶液的温度。 微阵列读取器还包括传感器，其接收由缓冲溶液中激发的分子发射的荧光以产生信号。

    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR
    5.
    发明申请
    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR 有权
    基于EVANESCENT WAVE生物传感器的微型读取器样品座

    公开(公告)号：US20130130937A1

    公开(公告)日：2013-05-23

    申请号：US13697453

    申请日：2010-05-20

    Applicant: Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    Inventor： Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    IPC: C12Q1/68

    CPC classification number: G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    Abstract: A microarray reader (500) includes a light source (501) which emits light and beam shaping elements (502) positioned near the light source (501) to direct the light. The microarray reader (500) further includes a microarray (504) that is at least formed of an optical substrate (505) and a reaction chamber (506) in contact with the optical substrate (505). A buffer solution (507) is encapsulated by the optical substrate (505) and the reaction chamber (506) and a holder (508) aligns the microarray (504) relative to the light source (501) such that when light hits the buffer solution (507) molecules in the buffer solution (507) are excited to emit fluorescent light. A temperature control component (509) is thermally engaged with the holder (508) such that the temperature control component (509) adjusts the temperature of the buffer solution (507). The microarray reader (500) further includes a sensor (510) that receives the fluorescent light emitted by the excited molecules in the buffer solution (507) to generate a signal. The microarray reader (500) provides a simple and low-cost solution to overcome the drawback of improper alignment of the microarray (504) on the temperature control component (509).

    Abstract translation: 微阵列读取器（500）包括发射光的光源（501）和位于光源（501）附近的引导光的光束成形元件（502）。 微阵列读取器（500）还包括微阵列（504），其至少由与光学基板（505）接触的光学基板（505）和反应室（506）形成。 缓冲溶液（507）由光学基底（505）和反应室（506）封装，并且保持器（508）相对于光源（501）对准微阵列（504），使得当光照射缓冲溶液 缓冲溶液（507）中的（507）分子被激发以发射荧光。 温度控制部件（509）与保持器（508）热接合，使得温度控制部件（509）调节缓冲溶液（507）的温度。 微阵列读取器（500）还包括传感器（510），其接收由缓冲溶液（507）中的激发分子发射的荧光以产生信号。 微阵列读取器（500）提供了简单且低成本的解决方案，以克服微阵列（504）在温度控制部件（509）上的不正确对准的缺点。

    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL
    6.
    发明申请
    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL 有权
    通过石墨材料提高实时荧光定量PCR检测效率的方法

    公开(公告)号：US20130040295A1

    公开(公告)日：2013-02-14

    申请号：US13522938

    申请日：2010-01-20

    Applicant: Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    Inventor： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC: C12M1/34 ,  G01N21/64

    CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    Abstract: A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    Abstract translation: 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING
    8.
    发明申请
    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING 审中-公开
    LUCIFERIN-LUCIFERASE基于微生物鉴定的MICRODEVICE

    公开(公告)号：US20110091916A1

    公开(公告)日：2011-04-21

    申请号：US12988402

    申请日：2008-04-21

    Applicant: Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    Inventor： Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    IPC: C12Q1/66 ,  C12M1/34

    CPC classification number: G01N21/763

    Abstract: A method and apparatus for determining the concentration of one or more microbes in a sample is provided. This method involves filtering a sample through a filter inside a sample tube to retain the one or more microbes on the filter. The resulting filtrate, which contains or produces adenosine triphosphate, is passed through the sample tube and enters the reporter region. In the reporter region, the adenosine triphosphate in the filtrate comes in contact with a transparent porous matrix, which includes a luciferin-luciferase complex. The adenosine triphosphate interacts with the luciferin-luciferase complex to provide light response, which is measured by a detector. The light response is compared with a calibration curve to determine the total concentration of one or more microbes in a sample.

    Abstract translation: 提供了用于确定样品中一种或多种微生物浓度的方法和装置。 该方法包括通过样品管内的过滤器过滤样品以将一种或多种微生物保留在过滤器上。 将含有或产生三磷酸腺苷的所得滤液通过样品管并进入报道区。 在报道区域中，滤液中的三磷酸腺苷与透明的多孔基质接触，该基质包括荧光素 - 荧光素酶复合物。 三磷酸腺苷与荧光素 - 萤光素酶复合物相互作用以提供光响应，其由检测器测量。 将光响应与校准曲线进行比较，以确定样品中一种或多种微生物的总浓度。

    Apparatus and method of using porous material for antigen separation, identification, and quantification with electrophoresis techniques
    9.
    发明授权
    Apparatus and method of using porous material for antigen separation, identification, and quantification with electrophoresis techniques 有权
    使用多孔材料进行抗原分离，鉴定和电泳技术定量的装置和方法

    公开(公告)号：US08105844B2

    公开(公告)日：2012-01-31

    申请号：US12123714

    申请日：2008-05-20

    Applicant: Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    Inventor： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    IPC: G01N33/563

    CPC classification number: B01D57/02 ,  G01N33/582

    Abstract: An apparatus includes a porous membrane for retaining antigens from a sample as the sample passes through the membrane. The apparatus also includes a first binding region within the membrane. The first binding region includes antibodies associated with a first antigen of interest. At least some of the antigens retained in the membrane are brought into contact with the first binding region by applying an electrophoresis field across the membrane. The porous membrane could also include an electrophoresis buffer. A presence of the first antigen of interest could be detected by exposing the first binding region to a chemiluminescent reagent, and a quantity of the first antigen of interest could be determined by performing a chemiluminescent assay on the binding region.

    Abstract translation: 一种装置包括当样品通过膜时用于保留来自样品的抗原的多孔膜。 该装置还包括膜内的第一结合区域。 第一结合区包括与感兴趣的第一抗原相关的抗体。 通过在膜上施加电泳场，保留在膜中的至少一些抗原与第一结合区接触。 多孔膜还可以包括电泳缓冲液。 可以通过将第一结合区域暴露于化学发光试剂来检测感兴趣的第一抗原的存在，并且可以通过在结合区域上进行化学发光测定来确定一定数量的目的第一抗原。

    APPARATUS AND METHOD OF USING POROUS MATERIAL FOR ANTIGEN SEPARATION, IDENTIFICATION, AND QUANTIFICATION WITH ELECTROPHORESIS TECHNIQUES
    10.
    发明申请
    APPARATUS AND METHOD OF USING POROUS MATERIAL FOR ANTIGEN SEPARATION, IDENTIFICATION, AND QUANTIFICATION WITH ELECTROPHORESIS TECHNIQUES 有权
    使用多孔材料进行抗电离技术的装置和方法进行抗分离，鉴定和定量

    公开(公告)号：US20090288953A1

    公开(公告)日：2009-11-26

    申请号：US12123714

    申请日：2008-05-20

    Applicant: Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    Inventor： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    IPC: B01D57/02

    CPC classification number: B01D57/02 ,  G01N33/582

    Abstract: An apparatus includes a porous membrane for retaining antigens from a sample as the sample passes through the membrane. The apparatus also includes a first binding region within the membrane. The first binding region includes antibodies associated with a first antigen of interest. At least some of the antigens retained in the membrane are brought into contact with the first binding region by applying an electrophoresis field across the membrane. The porous membrane could also include an electrophoresis buffer. A presence of the first antigen of interest could be detected by exposing the first binding region to a chemiluminescent reagent, and a quantity of the first antigen of interest could be determined by performing a chemiluminescent assay on the binding region.

    Abstract translation: 一种装置包括当样品通过膜时用于保留来自样品的抗原的多孔膜。 该装置还包括膜内的第一结合区域。 第一结合区包括与感兴趣的第一抗原相关的抗体。 通过在膜上施加电泳场，保留在膜中的至少一些抗原与第一结合区接触。 多孔膜还可以包括电泳缓冲液。 可以通过将第一结合区域暴露于化学发光试剂来检测感兴趣的第一抗原的存在，并且可以通过在结合区域上进行化学发光测定来确定一定数量的目的第一抗原。

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