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    Method to increase detection efficiency of real time PCR microarray by quartz material
    2.
    发明授权
    Method to increase detection efficiency of real time PCR microarray by quartz material 有权
    通过石英材料提高实时PCR微阵列检测效率的方法

    公开(公告)号：US09539571B2

    公开(公告)日：2017-01-10

    申请号：US13522938

    申请日：2010-01-20

    申请人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    发明人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

    CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    摘要： A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    摘要翻译： 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    Microarray reader based on evanescent wave detection
    3.
    发明授权
    Microarray reader based on evanescent wave detection 有权
    基于ev逝波检测的微阵列阅读器

    公开(公告)号：US08809810B2

    公开(公告)日：2014-08-19

    申请号：US13697453

    申请日：2010-05-20

    申请人： Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    发明人： Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    IPC分类号： G01N21/64

    CPC分类号： G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    摘要： The disclosure relates to a microarray reader that includes a light source which emits light and beam shaping elements positioned near the light source to direct the light. The microarray reader further includes a microarray that is at least formed of an optical substrate and a reaction chamber in contact with the optical substrate. A buffer solution is encapsulated by the optical substrate and the reaction chamber and a holder aligns the microarray relative to the light source such that when light hits the buffer solution molecules in the buffer solution are excited to emit fluorescent light. A temperature control component is thermally engaged with the holder such that the temperature control component adjusts the temperature of the buffer solution. The microarray reader further includes a sensor that receives the fluorescent light emitted by the excited molecules in the buffer solution to generate a signal.

    摘要翻译： 本发明涉及一种微阵列读取器，其包括发射光的光源和位于光源附近的光束成形元件以引导光。 微阵列读取器还包括至少由光学基底和与光学基底接触的反应室形成的微阵列。 缓冲溶液被光学基底和反应室封装，并且保持器将微阵列相对于光源对准，使得当光照射缓冲溶液时，激发缓冲溶液中的分子发射荧光。 温度控制部件与保持器热接合，使得温度控制部件调节缓冲溶液的温度。 微阵列读取器还包括传感器，其接收由缓冲溶液中激发的分子发射的荧光以产生信号。

    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR
    4.
    发明申请
    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR 有权
    基于EVANESCENT WAVE生物传感器的微型读取器样品座

    公开(公告)号：US20130130937A1

    公开(公告)日：2013-05-23

    申请号：US13697453

    申请日：2010-05-20

    申请人： Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    发明人： Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    IPC分类号： C12Q1/68

    CPC分类号： G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    摘要： A microarray reader (500) includes a light source (501) which emits light and beam shaping elements (502) positioned near the light source (501) to direct the light. The microarray reader (500) further includes a microarray (504) that is at least formed of an optical substrate (505) and a reaction chamber (506) in contact with the optical substrate (505). A buffer solution (507) is encapsulated by the optical substrate (505) and the reaction chamber (506) and a holder (508) aligns the microarray (504) relative to the light source (501) such that when light hits the buffer solution (507) molecules in the buffer solution (507) are excited to emit fluorescent light. A temperature control component (509) is thermally engaged with the holder (508) such that the temperature control component (509) adjusts the temperature of the buffer solution (507). The microarray reader (500) further includes a sensor (510) that receives the fluorescent light emitted by the excited molecules in the buffer solution (507) to generate a signal. The microarray reader (500) provides a simple and low-cost solution to overcome the drawback of improper alignment of the microarray (504) on the temperature control component (509).

    摘要翻译： 微阵列读取器（500）包括发射光的光源（501）和位于光源（501）附近的引导光的光束成形元件（502）。 微阵列读取器（500）还包括微阵列（504），其至少由与光学基板（505）接触的光学基板（505）和反应室（506）形成。 缓冲溶液（507）由光学基底（505）和反应室（506）封装，并且保持器（508）相对于光源（501）对准微阵列（504），使得当光照射缓冲溶液 缓冲溶液（507）中的（507）分子被激发以发射荧光。 温度控制部件（509）与保持器（508）热接合，使得温度控制部件（509）调节缓冲溶液（507）的温度。 微阵列读取器（500）还包括传感器（510），其接收由缓冲溶液（507）中的激发分子发射的荧光以产生信号。 微阵列读取器（500）提供了简单且低成本的解决方案，以克服微阵列（504）在温度控制部件（509）上的不正确对准的缺点。

    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL
    5.
    发明申请
    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL 有权
    通过石墨材料提高实时荧光定量PCR检测效率的方法

    公开(公告)号：US20130040295A1

    公开(公告)日：2013-02-14

    申请号：US13522938

    申请日：2010-01-20

    申请人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    发明人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC分类号： C12M1/34 ,  G01N21/64

    CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    摘要： A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    摘要翻译： 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING
    8.
    发明申请
    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING 审中-公开
    LUCIFERIN-LUCIFERASE基于微生物鉴定的MICRODEVICE

    公开(公告)号：US20110091916A1

    公开(公告)日：2011-04-21

    申请号：US12988402

    申请日：2008-04-21

    申请人： Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    发明人： Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    IPC分类号： C12Q1/66 ,  C12M1/34

    CPC分类号： G01N21/763

    摘要： A method and apparatus for determining the concentration of one or more microbes in a sample is provided. This method involves filtering a sample through a filter inside a sample tube to retain the one or more microbes on the filter. The resulting filtrate, which contains or produces adenosine triphosphate, is passed through the sample tube and enters the reporter region. In the reporter region, the adenosine triphosphate in the filtrate comes in contact with a transparent porous matrix, which includes a luciferin-luciferase complex. The adenosine triphosphate interacts with the luciferin-luciferase complex to provide light response, which is measured by a detector. The light response is compared with a calibration curve to determine the total concentration of one or more microbes in a sample.

    摘要翻译： 提供了用于确定样品中一种或多种微生物浓度的方法和装置。 该方法包括通过样品管内的过滤器过滤样品以将一种或多种微生物保留在过滤器上。 将含有或产生三磷酸腺苷的所得滤液通过样品管并进入报道区。 在报道区域中，滤液中的三磷酸腺苷与透明的多孔基质接触，该基质包括荧光素 - 荧光素酶复合物。 三磷酸腺苷与荧光素 - 萤光素酶复合物相互作用以提供光响应，其由检测器测量。 将光响应与校准曲线进行比较，以确定样品中一种或多种微生物的总浓度。

    End of service life indicator for organic vapor respirator filter cartridge
    10.
    发明授权
    End of service life indicator for organic vapor respirator filter cartridge 有权
    有机蒸汽呼吸器滤芯使用寿命指示器

    公开(公告)号：US08859995B2

    公开(公告)日：2014-10-14

    申请号：US13536019

    申请日：2012-06-28

    申请人： Hailin Liu ,  Tao Pan ,  Xuanbin Liu ,  Li Wang

    发明人： Hailin Liu ,  Tao Pan ,  Xuanbin Liu ,  Li Wang

    IPC分类号： G01J1/58

    CPC分类号： A62B9/006 ,  A62B18/088 ,  G01N21/77 ,  G01N2021/7786

    摘要： Embodiments generally relate to detection of end of service life for respirator filter cartridges for organic vapor(s). Typically, detection of end of service life might use a competitive UV absorbance-fluorescence approach. Thus, a sensor that emits light upon application of UV may be placed within the cartridge, with a UV lamp and a light detector directed at the sensor. In some embodiments, a plurality of corresponding sensor and UV lamp and light detector might be used. Typically, the light level emitted by the sensor(s) might be used to estimate effective end of service life.

    摘要翻译： 实施例通常涉及用于有机蒸气的呼吸器滤芯的使用寿命终止的检测。 通常，检测使用寿命可能会使用竞争性的紫外吸收 - 荧光法。 因此，在施加UV时发出光的传感器可以被放置在盒内，UV灯和指向传感器的光检测器。 在一些实施例中，可以使用多个对应的传感器和UV灯和光检测器。 通常，由传感器发射的光级可用于估计有效的使用寿命。