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    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR
    3.
    发明申请
    SAMPLE HOLDER FOR MICROARRAY READER BASED ON EVANESCENT WAVE BIOSENSOR 有权
    基于EVANESCENT WAVE生物传感器的微型读取器样品座

    公开(公告)号：US20130130937A1

    公开(公告)日：2013-05-23

    申请号：US13697453

    申请日：2010-05-20

    申请人： Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    发明人： Zhenhong Sun ,  Xuanbin Liu ,  Tao Pan

    IPC分类号： C12Q1/68

    CPC分类号： G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    摘要： A microarray reader (500) includes a light source (501) which emits light and beam shaping elements (502) positioned near the light source (501) to direct the light. The microarray reader (500) further includes a microarray (504) that is at least formed of an optical substrate (505) and a reaction chamber (506) in contact with the optical substrate (505). A buffer solution (507) is encapsulated by the optical substrate (505) and the reaction chamber (506) and a holder (508) aligns the microarray (504) relative to the light source (501) such that when light hits the buffer solution (507) molecules in the buffer solution (507) are excited to emit fluorescent light. A temperature control component (509) is thermally engaged with the holder (508) such that the temperature control component (509) adjusts the temperature of the buffer solution (507). The microarray reader (500) further includes a sensor (510) that receives the fluorescent light emitted by the excited molecules in the buffer solution (507) to generate a signal. The microarray reader (500) provides a simple and low-cost solution to overcome the drawback of improper alignment of the microarray (504) on the temperature control component (509).

    摘要翻译： 微阵列读取器（500）包括发射光的光源（501）和位于光源（501）附近的引导光的光束成形元件（502）。 微阵列读取器（500）还包括微阵列（504），其至少由与光学基板（505）接触的光学基板（505）和反应室（506）形成。 缓冲溶液（507）由光学基底（505）和反应室（506）封装，并且保持器（508）相对于光源（501）对准微阵列（504），使得当光照射缓冲溶液 缓冲溶液（507）中的（507）分子被激发以发射荧光。 温度控制部件（509）与保持器（508）热接合，使得温度控制部件（509）调节缓冲溶液（507）的温度。 微阵列读取器（500）还包括传感器（510），其接收由缓冲溶液（507）中的激发分子发射的荧光以产生信号。 微阵列读取器（500）提供了简单且低成本的解决方案，以克服微阵列（504）在温度控制部件（509）上的不正确对准的缺点。

    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL
    4.
    发明申请
    METHOD TO INCREASE DETECTION EFFICIENCY OF REAL TIME PCR MICROARRAY BY QUARTZ MATERIAL 有权
    通过石墨材料提高实时荧光定量PCR检测效率的方法

    公开(公告)号：US20130040295A1

    公开(公告)日：2013-02-14

    申请号：US13522938

    申请日：2010-01-20

    申请人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    发明人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC分类号： C12M1/34 ,  G01N21/64

    CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    摘要： A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    摘要翻译： 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    Method to increase detection efficiency of real time PCR microarray by quartz material
    5.
    发明授权
    Method to increase detection efficiency of real time PCR microarray by quartz material 有权
    通过石英材料提高实时PCR微阵列检测效率的方法

    公开(公告)号：US09539571B2

    公开(公告)日：2017-01-10

    申请号：US13522938

    申请日：2010-01-20

    申请人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    发明人： Zhenhong Sun ,  Tao Pan ,  Xuanbin Liu

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

    CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816

    摘要： A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

    摘要翻译： 提供了使用ev逝波检测技术定量分析靶核酸的反应器和用于定量分析靶核酸的方法。 反应器包括具有空腔的衬底，布置在衬底上的缓冲层，布置在缓冲层上的石英盖板以及入口和出口。 反应器对于PCR处理是热和化学稳定的并且适用于ev逝波检测技术。

    Microarray reader based on evanescent wave detection
    6.
    发明授权
    Microarray reader based on evanescent wave detection 有权
    基于ev逝波检测的微阵列阅读器

    公开(公告)号：US08809810B2

    公开(公告)日：2014-08-19

    申请号：US13697453

    申请日：2010-05-20

    申请人： Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    发明人： Xuanbin Liu ,  Tao Pan ,  Zhenhong Sun

    IPC分类号： G01N21/64

    CPC分类号： G01N21/64 ,  C12Q1/686 ,  G01N21/6452 ,  G01N21/648

    摘要： The disclosure relates to a microarray reader that includes a light source which emits light and beam shaping elements positioned near the light source to direct the light. The microarray reader further includes a microarray that is at least formed of an optical substrate and a reaction chamber in contact with the optical substrate. A buffer solution is encapsulated by the optical substrate and the reaction chamber and a holder aligns the microarray relative to the light source such that when light hits the buffer solution molecules in the buffer solution are excited to emit fluorescent light. A temperature control component is thermally engaged with the holder such that the temperature control component adjusts the temperature of the buffer solution. The microarray reader further includes a sensor that receives the fluorescent light emitted by the excited molecules in the buffer solution to generate a signal.

    摘要翻译： 本发明涉及一种微阵列读取器，其包括发射光的光源和位于光源附近的光束成形元件以引导光。 微阵列读取器还包括至少由光学基底和与光学基底接触的反应室形成的微阵列。 缓冲溶液被光学基底和反应室封装，并且保持器将微阵列相对于光源对准，使得当光照射缓冲溶液时，激发缓冲溶液中的分子发射荧光。 温度控制部件与保持器热接合，使得温度控制部件调节缓冲溶液的温度。 微阵列读取器还包括传感器，其接收由缓冲溶液中激发的分子发射的荧光以产生信号。

    Programmable oligonucleotide micro array
    9.
    发明授权
    Programmable oligonucleotide micro array 有权
    可编程寡核苷酸微阵列

    公开(公告)号：US09005931B2

    公开(公告)日：2015-04-14

    申请号：US12810078

    申请日：2007-12-24

    申请人： Zhenhong Sun ,  Wendy Wang ,  Hang Liao

    发明人： Zhenhong Sun ,  Wendy Wang ,  Hang Liao

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  B01L7/00

    CPC分类号： C12Q1/6837 ,  B01L7/52 ,  C12Q1/686 ,  Y02A50/57 ,  C12Q2565/628 ,  C12Q2561/113 ,  C12Q2537/143 ,  C12Q2527/107

    摘要： A programmable probe design of DNA micro array and detection methodology is provided. DNA probes, which are complemented with the target DNA, are designed and classified into groups according to optimum hybridization temperature. The probes are arrayed by the group and immobilized on the substrate surface of the DNA micro array. The control system, imaging system and temperature control system are programmed to cooperate with each other during the detection process. This design increases the detection capabilities of the parallel-analysis system.

    摘要翻译： 提供了DNA微阵列和检测方法的可编程探针设计。 根据最适杂交温度，设计与靶DNA互补的DNA探针。 探针由组排列并固定在DNA微阵列的底物表面上。 控制系统，成像系统和温度控制系统被编程为在检测过程中相互配合。 该设计增加了并行分析系统的检测能力。

    Reaction buffer for microarray
    10.
    发明授权
    Reaction buffer for microarray 有权

    公开(公告)号：US09657337B2

    公开(公告)日：2017-05-23

    申请号：US12520987

    申请日：2006-12-29

    申请人： Leon Xu ,  Zhenhong Sun

    发明人： Leon Xu ,  Zhenhong Sun

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6837 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2565/501

    摘要： Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-200 mM of a salt, about 10-30 mM Tris-HCl, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-150 uM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.