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1.发明授权Hepatitis C virus antigen polypeptide, production method therefor, and antibody detection method 失效丙型肝炎病毒抗原多肽，其制备方法和抗体检测方法
公开(公告)号：US5714314A
公开(公告)日：1998-02-03
申请号：US452287
申请日：1995-05-26
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
IPC分类号： G01N33/576 , A61K39/29 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/195 , C12N15/09 , C12N15/51 , C12P21/00 , C12P21/02 , C12R1/91 , C12Q1/70 , G01N33/53 , G01N33/68
CPC分类号： C07K14/005 , C12N2710/14143 , C12N2770/24222 , Y10S530/826 , Y10S930/223
摘要： A hepatitis C virus antigen polypeptide having a molecular weight of approximately 22 kilodaltons expressed from a hepatitis C virus structural gene region; a production method for a hepatitis C virus antigen polypeptide having a molecular weight of 22 kilodaltons and/or a peptide related thereto, wherein an expression vector having inserted thereinto a cDNA fragment of a hepatitis C virus structural gene region is inserted into a cultured cell line and the transfected cell line thus obtained is cultured; and a detection method for a hepatitis C virus antibody, wherein a hepatitis C virus antigen polypeptide is used as an antigen, and an antibody specific thereto is detected.
摘要翻译： 由丙型肝炎病毒结构基因区域表达的分子量约22千道尔顿的丙型肝炎病毒抗原多肽; 一种分子量为22千道尔顿的丙型肝炎病毒抗原多肽和/或与其相关的肽的制备方法，其中将插入有丙型肝炎病毒结构基因区域的cDNA片段的表达载体插入培养的细胞系 培养由此获得的转染细胞系; 以及丙型肝炎病毒抗体的检测方法，其中使用丙型肝炎病毒抗原多肽作为抗原，并检测其特异性抗体。
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2.发明授权Hepatitis C virus antigen polypeptide, production method therefor, and antibody detection method 失效丙型肝炎病毒抗原多肽，其制备方法和抗体检测方法
公开(公告)号：US5734019A
公开(公告)日：1998-03-31
申请号：US952543
申请日：1992-12-11
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
IPC分类号： G01N33/576 , A61K39/29 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/195 , C12N15/09 , C12N15/51 , C12P21/00 , C12P21/02 , C12R1/91 , G01N33/569
CPC分类号： C07K14/005 , C12N2710/14143 , C12N2770/24222 , Y10S530/826 , Y10S930/223
摘要： A hepatitis C virus antigen polypeptide having a molecular weight of approximately 22 kilodaltons expressed from a hepatitis C virus structural gene region; a production method for a hepatitis C virus antigen polypeptide having a molecular weight of 22 kilodaltons and/or a peptide related thereto, wherein an expression vector having inserted thereinto a cDNA fragment of a hepatitis C virus structural gene region is inserted into a cultured cell line and the transfected cell line thus obtained is cultured; and a detection method for a hepatitis C virus antibody, wherein a hepatitis C virus antigen polypeptide is used as an antigen, and an antibody specific thereto is detected.
摘要翻译： PCT No.PCT / JP91 / 00724 Sec。 371日期：1992年12月11日 102（e）日期1992年12月11日PCT 1991年5月29日PCT PCT。 出版物WO91 / 19744 1991年12月26日，由丙型肝炎病毒结构基因区域表达的分子量约22千道尔顿的丙型肝炎病毒抗原多肽; 一种分子量为22千道尔顿的丙型肝炎病毒抗原多肽和/或与其相关的肽的制备方法，其中将插入有丙型肝炎病毒结构基因区域的cDNA片段的表达载体插入培养的细胞系 培养由此获得的转染细胞系; 以及丙型肝炎病毒抗体的检测方法，其中使用丙型肝炎病毒抗原多肽作为抗原，并检测其特异性抗体。
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3.发明授权Antigenic peptide for detecting anti-hepatitis C virus antibodies and use thereof 失效用于检测抗丙型肝炎病毒抗体的抗原肽及其用途
公开(公告)号：US5302507A
公开(公告)日：1994-04-12
申请号：US744427
申请日：1991-08-13
申请人： Joe Chiba , Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura
发明人： Joe Chiba , Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura
IPC分类号： G01N33/576 , A61K39/29 , C07K7/08 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C07K16/10 , C12Q1/70
CPC分类号： C07K14/005 , C12N2770/24222 , Y10S436/82
摘要： The antigenic peptide represented by the following amino acid sequence (SEQ ID NO: 1) ##STR1## and a method of the detection of anti-HCV antibodies wherein the above peptide is brought into contact with a sample under the conditions that the peptide is bound to anti-HCV antibodies present in the sample to form an immunological complex and the formation of the immunological complex is measured to confirm the existence of the anti HCV antibodies in the sample. The method is highly specific and sensitive to anti-HCV antibodies.
摘要翻译： 由以下氨基酸序列（SEQ ID NO：1）表示的抗原肽（*化学结构*）和检测抗HCV抗体的方法，其中上述肽在样品的条件下与样品接触 肽与样品中存在的抗HCV抗体结合以形成免疫复合物，并测量免疫复合物的形成以证实样品中抗HCV抗体的存在。 该方法对抗HCV抗体具有高度的特异性和敏感性。
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公开(公告)号：US5747241A
公开(公告)日：1998-05-05
申请号：US460806
申请日：1995-06-02
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576 , C12P21/02
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, comprising the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provides a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种用于检测由丙型肝炎病毒感染引起的抗体的丙型肝炎诊断试剂，其包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
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公开(公告)号：US5750331A
公开(公告)日：1998-05-12
申请号：US325630
申请日：1994-10-19
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, and comprises the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provide a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种丙型肝炎诊断试剂，其检测由丙型肝炎病毒感染引起的抗体，并且包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
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公开(公告)号：US6063904A
公开(公告)日：2000-05-16
申请号：US127829
申请日：1998-08-03
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
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公开(公告)号：US5830691A
公开(公告)日：1998-11-03
申请号：US636883
申请日：1996-04-24
IPC分类号： C12N15/09 , A61K39/00 , C07K14/18 , C12N15/51 , C12P21/02 , C12R1/91 , C12P21/06 , C07H19/00 , C07K1/00 , C12N5/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
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公开(公告)号：US5789544A
公开(公告)日：1998-08-04
申请号：US462195
申请日：1995-06-05
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
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公开(公告)号：US07101551B2
公开(公告)日：2006-09-05
申请号：US10203754
申请日：2001-02-13
申请人： Seima Itami , Tatsurou Shibui , Makoto Seki , Yoshihisa Yotsumoto , Yoshiharu Matsuura , Tatsuo Miyamura
发明人： Seima Itami , Tatsurou Shibui , Makoto Seki , Yoshihisa Yotsumoto , Yoshiharu Matsuura , Tatsuo Miyamura
CPC分类号： A61K31/737 , A61K2039/505 , C07K16/109 , C07K2317/21 , C07K2317/622
摘要： The present invention provides a substance which inhibits the binding between E2/NS1 protein of hepatitis C virus and a cell infectious with hepatitis C virus, a cell expressing CD81, or CD81. The present invention can provide a novel medicament which has an anti-viral effects such as an inhibitory action against HCV infection.
摘要翻译： 本发明提供抑制丙型肝炎病毒的E2 / NS1蛋白与感染丙型肝炎病毒的细胞，表达CD81或CD81的细胞的结合的物质。 本发明可以提供具有抗HCV感染的抑制作用的抗病毒作用的新型药物。
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公开(公告)号：US20050053620A1
公开(公告)日：2005-03-10
申请号：US10495974
申请日：2001-11-20
申请人： Mitsuo Honda , Kazuhiro Matsuo , Takeaki Ohsu , Tatsuo Miyamura , Yoshiharu Matsuura , Koji Ishii , Kenzo Kato
发明人： Mitsuo Honda , Kazuhiro Matsuo , Takeaki Ohsu , Tatsuo Miyamura , Yoshiharu Matsuura , Koji Ishii , Kenzo Kato
CPC分类号： C12N7/00 , A61K39/00 , A61K2039/5256 , A61K2039/53 , C07K14/005 , C12N15/86 , C12N2710/24043 , C12N2740/16122
摘要： The invention provides a recombinant vaccinia virus strain DIs that possesses a polynucleotide encoding a foreign antigenic protein in the non-essential gene region of the chromosome DNA and expresses the antigenic protein; and provides a highly-safe, vaccinia virus vaccine containing the recombinant virus strain DIs as the active ingredient. The invention also provides a method of using the vaccinia virus strain DIs as a vector for protein expression.
摘要翻译： 本发明提供了一种重组痘苗病毒株DIs，其具有编码染色体DNA非必需基因区域中外源抗原蛋白的多核苷酸并表达该抗原蛋白; 并提供含有重组病毒株DIs作为活性成分的高度安全的痘苗病毒疫苗。 本发明还提供了使用痘苗病毒株DIs作为蛋白质表达载体的方法。
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