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1.发明授权Hepatitis C virus antigen polypeptide, production method therefor, and antibody detection method 失效丙型肝炎病毒抗原多肽，其制备方法和抗体检测方法
公开(公告)号：US5714314A
公开(公告)日：1998-02-03
申请号：US452287
申请日：1995-05-26
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
IPC分类号： G01N33/576 , A61K39/29 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/195 , C12N15/09 , C12N15/51 , C12P21/00 , C12P21/02 , C12R1/91 , C12Q1/70 , G01N33/53 , G01N33/68
CPC分类号： C07K14/005 , C12N2710/14143 , C12N2770/24222 , Y10S530/826 , Y10S930/223
摘要： A hepatitis C virus antigen polypeptide having a molecular weight of approximately 22 kilodaltons expressed from a hepatitis C virus structural gene region; a production method for a hepatitis C virus antigen polypeptide having a molecular weight of 22 kilodaltons and/or a peptide related thereto, wherein an expression vector having inserted thereinto a cDNA fragment of a hepatitis C virus structural gene region is inserted into a cultured cell line and the transfected cell line thus obtained is cultured; and a detection method for a hepatitis C virus antibody, wherein a hepatitis C virus antigen polypeptide is used as an antigen, and an antibody specific thereto is detected.
摘要翻译： 由丙型肝炎病毒结构基因区域表达的分子量约22千道尔顿的丙型肝炎病毒抗原多肽; 一种分子量为22千道尔顿的丙型肝炎病毒抗原多肽和/或与其相关的肽的制备方法，其中将插入有丙型肝炎病毒结构基因区域的cDNA片段的表达载体插入培养的细胞系 培养由此获得的转染细胞系; 以及丙型肝炎病毒抗体的检测方法，其中使用丙型肝炎病毒抗原多肽作为抗原，并检测其特异性抗体。
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2.发明授权Hepatitis C virus antigen polypeptide, production method therefor, and antibody detection method 失效丙型肝炎病毒抗原多肽，其制备方法和抗体检测方法
公开(公告)号：US5734019A
公开(公告)日：1998-03-31
申请号：US952543
申请日：1992-12-11
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura , Joe Chiba
IPC分类号： G01N33/576 , A61K39/29 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/195 , C12N15/09 , C12N15/51 , C12P21/00 , C12P21/02 , C12R1/91 , G01N33/569
CPC分类号： C07K14/005 , C12N2710/14143 , C12N2770/24222 , Y10S530/826 , Y10S930/223
摘要： A hepatitis C virus antigen polypeptide having a molecular weight of approximately 22 kilodaltons expressed from a hepatitis C virus structural gene region; a production method for a hepatitis C virus antigen polypeptide having a molecular weight of 22 kilodaltons and/or a peptide related thereto, wherein an expression vector having inserted thereinto a cDNA fragment of a hepatitis C virus structural gene region is inserted into a cultured cell line and the transfected cell line thus obtained is cultured; and a detection method for a hepatitis C virus antibody, wherein a hepatitis C virus antigen polypeptide is used as an antigen, and an antibody specific thereto is detected.
摘要翻译： PCT No.PCT / JP91 / 00724 Sec。 371日期：1992年12月11日 102（e）日期1992年12月11日PCT 1991年5月29日PCT PCT。 出版物WO91 / 19744 1991年12月26日，由丙型肝炎病毒结构基因区域表达的分子量约22千道尔顿的丙型肝炎病毒抗原多肽; 一种分子量为22千道尔顿的丙型肝炎病毒抗原多肽和/或与其相关的肽的制备方法，其中将插入有丙型肝炎病毒结构基因区域的cDNA片段的表达载体插入培养的细胞系 培养由此获得的转染细胞系; 以及丙型肝炎病毒抗体的检测方法，其中使用丙型肝炎病毒抗原多肽作为抗原，并检测其特异性抗体。
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3.发明授权Antigenic peptide for detecting anti-hepatitis C virus antibodies and use thereof 失效用于检测抗丙型肝炎病毒抗体的抗原肽及其用途
公开(公告)号：US5302507A
公开(公告)日：1994-04-12
申请号：US744427
申请日：1991-08-13
申请人： Joe Chiba , Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura
发明人： Joe Chiba , Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshiharu Matsuura
IPC分类号： G01N33/576 , A61K39/29 , C07K7/08 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C07K16/10 , C12Q1/70
CPC分类号： C07K14/005 , C12N2770/24222 , Y10S436/82
摘要： The antigenic peptide represented by the following amino acid sequence (SEQ ID NO: 1) ##STR1## and a method of the detection of anti-HCV antibodies wherein the above peptide is brought into contact with a sample under the conditions that the peptide is bound to anti-HCV antibodies present in the sample to form an immunological complex and the formation of the immunological complex is measured to confirm the existence of the anti HCV antibodies in the sample. The method is highly specific and sensitive to anti-HCV antibodies.
摘要翻译： 由以下氨基酸序列（SEQ ID NO：1）表示的抗原肽（*化学结构*）和检测抗HCV抗体的方法，其中上述肽在样品的条件下与样品接触 肽与样品中存在的抗HCV抗体结合以形成免疫复合物，并测量免疫复合物的形成以证实样品中抗HCV抗体的存在。 该方法对抗HCV抗体具有高度的特异性和敏感性。
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公开(公告)号：US5750331A
公开(公告)日：1998-05-12
申请号：US325630
申请日：1994-10-19
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, and comprises the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provide a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种丙型肝炎诊断试剂，其检测由丙型肝炎病毒感染引起的抗体，并且包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
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公开(公告)号：US5747241A
公开(公告)日：1998-05-05
申请号：US460806
申请日：1995-06-02
申请人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
发明人： Tatsuo Miyamura , Izumu Saito , Shizuko Harada , Yoshikazu Honda
IPC分类号： C07K14/18 , C12N15/09 , G01N33/576 , C12P21/02
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： A diagnostic reagent for hepatitis C, which detects an antibody induced by infection of hepatitis C virus, comprising the second envelope protein or first non-structural protein which is encoded by the gene of hepatitis C virus and has a sugar chain. This invention also provides a method for detecting an anti-hepatitis C virus antibody. The use of the diagnostic reagent for hepatitis C according to the present invention makes highly sensitive diagnosis of hepatitis C possible.
摘要翻译： 一种用于检测由丙型肝炎病毒感染引起的抗体的丙型肝炎诊断试剂，其包含由丙型肝炎病毒基因编码并具有糖链的第二包膜蛋白或第一非结构蛋白。 本发明还提供了检测抗丙型肝炎病毒抗体的方法。 根据本发明的丙型肝炎诊断试剂的使用使丙型肝炎的高度敏感性诊断成为可能。
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公开(公告)号：US6063904A
公开(公告)日：2000-05-16
申请号：US127829
申请日：1998-08-03
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
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公开(公告)号：US5830691A
公开(公告)日：1998-11-03
申请号：US636883
申请日：1996-04-24
IPC分类号： C12N15/09 , A61K39/00 , C07K14/18 , C12N15/51 , C12P21/02 , C12R1/91 , C12P21/06 , C07H19/00 , C07K1/00 , C12N5/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
摘要翻译： 一种细胞外产生丙型肝炎病毒的蛋白质的方法包括以下步骤：培养转化体，该转化体用含有编码丙型肝炎病毒蛋白的DNA片段的表达载体转化，并回收由转化体细胞外产生的丙型肝炎病毒的蛋白质 。 起源于通过该方法制备的E1区域的蛋白质可以用作制备用于预防HCV感染的疫苗的材料。 此外，含有蛋白质的诊断剂可用于HCV抗体的检测或其在血清等中的确认。 换句话说，本发明的蛋白质能够以高特异性和灵敏度诊断C型肝炎。
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公开(公告)号：US5789544A
公开(公告)日：1998-08-04
申请号：US462195
申请日：1995-06-05
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , C12N2770/36122
摘要： A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.
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公开(公告)号：US08216833B2
公开(公告)日：2012-07-10
申请号：US10553639
申请日：2003-11-19
申请人： Izumu Saito , Yumi Saito
发明人： Izumu Saito , Yumi Saito
IPC分类号： C12N15/861 , C12N15/70
CPC分类号： C12N15/86 , C12N15/70 , C12N2710/10343
摘要： A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.
摘要翻译： 提供了有效用于产生重组腺病毒载体的新型粘粒载体等。 更具体地，提供了一种粘粒载体，其特征在于：（1）含有腺病毒基因组，其具有各自具有完整核苷酸序列的腺病毒反向末端重复序列，（2）在腺病毒E1基因区域中具有缺失，和（3）含有 腺病毒基因组两侧不存在的限制酶识别序列; 使用粘粒载体产生重组腺病毒载体的方法; 以及用于产生含有粘粒载体作为组分的重组腺病毒载体的试剂。
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公开(公告)号：US20120020924A1
公开(公告)日：2012-01-26
申请号：US13152766
申请日：2011-06-03
申请人： Michio Nakai , Kazuo Komiya , Masashi Murata , Naoki Tohdoh , Izumu Saito
发明人： Michio Nakai , Kazuo Komiya , Masashi Murata , Naoki Tohdoh , Izumu Saito
IPC分类号： A61K48/00
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10361 , C12N2800/30
摘要： The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of the recombinant adenovirus vector, or a gene therapy method using the recombinant adenovirus vector.
摘要翻译： 本发明提供了一种新的腺病毒载体，其通过抑制插入到腺病毒基因组中的外源启动子诱导腺病毒基因的表达来减轻其体内给药期间的炎症，以及生成载体的方法，细胞系 用于生产重组腺病毒载体，或使用重组腺病毒载体的基因治疗方法。
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