利索-全球专利检索

  1. Login
  2. Sign Up

Search Results

9 records (took 0.012 seconds)

    METHODS FOR TEMPERATURE-MEDIATED NESTED POLYMERASE CHAIN REACTION
    2.
    发明申请
    METHODS FOR TEMPERATURE-MEDIATED NESTED POLYMERASE CHAIN REACTION 审中-公开
    温度介导的聚合酶链反应的方法

    公开(公告)号：US20170044585A1

    公开(公告)日：2017-02-16

    申请号：US15235596

    申请日：2016-08-12

    Applicant: Tetracore, Inc.

    Inventor： William M. Nelson ,  Kyle Armantrout ,  Tracy Calvin Fecteau

    IPC: C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC classification number: C12Q1/689 ,  C12Q1/6844 ,  C12Q1/701 ,  C12Q2527/107 ,  C12Q2547/101 ,  C12Q2549/119 ,  C12Q2561/113 ,  C12Q2563/107

    Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles. During the second phase, fluorescence probes anneal to the template strands and are degraded by DNA polymerase to emit fluorescence signals for detection and/or quantification of the target nucleic acid. Methods for amplifying nucleic acid in accordance with the disclosure may be employed for nucleic acid amplification and detection in clinical and research settings.

    Abstract translation: 本公开的实施方案涉及用于扩增核酸的方法，包括两个步骤：制备包含靶核酸的反应混合物的第一步骤和在热循环器中处理反应混合物的第二步骤。 在加工步骤的第一阶段期间，热循环器可以被配置为将反应混合物加热到第一温度，并将反应混合物反复冷却至第二温度，持续第一多个循环。 在第一阶段，荧光探针不退火模板链，不发出荧光信号。 在加工步骤的第二阶段期间，热循环器可以将反应混合物加热至第三温度，并将反应混合物反复冷却至第四温度第二多个循环。 在第二阶段期间，荧光探针与模板链退火，并被DNA聚合酶降解以发射用于靶核酸的检测和/或定量的荧光信号。 根据本发明的用于扩增核酸的方法可用于临床和研究环境中的核酸扩增和检测。

    SELF-METERING OF FLUID INTO A REACTION CHAMBER
    4.
    发明申请
    SELF-METERING OF FLUID INTO A REACTION CHAMBER 审中-公开
    将流体自动计入反应室

    公开(公告)号：US20170065980A1

    公开(公告)日：2017-03-09

    申请号：US15255431

    申请日：2016-09-02

    Applicant: Tetracore, Inc.

    Inventor： William M. Nelson ,  Aymeric Randanne de Vazeille ,  Kyle Armantrout

    IPC: B01L7/00 ,  C12P19/34

    Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

    Abstract translation: 自动计量反应装置具有被配置为接受不同量的流体的样品储存器; 计量储存器，其被配置为样品储存器的子部分并保持流体的反应量; 反应室流体连接到计量储存器; 以及柱塞，其包括被配置为与所述计量储存器形成密封的末端，使得当所述柱塞与所述计量储存器接触时，所述流体的反应量被密封在所述计量储存器内。 柱塞可以构造成将来自计量储存器的流体的密封反应量插入反应室。

    Self-metering of fluid into a reaction chamber
    5.
    发明授权
    Self-metering of fluid into a reaction chamber 有权

    公开(公告)号：US12103006B2

    公开(公告)日：2024-10-01

    申请号：US17006363

    申请日：2020-08-28

    Applicant: Tetracore, Inc.

    Inventor： William M. Nelson ,  Aymeric Randanne de Vazeille ,  Kyle Armantrout

    IPC: B01L7/00

    CPC classification number: B01L7/52 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2400/0478

    Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

    Self-metering of fluid into a reaction chamber
    6.
    发明授权
    Self-metering of fluid into a reaction chamber 有权

    公开(公告)号：US10758908B2

    公开(公告)日：2020-09-01

    申请号：US15255431

    申请日：2016-09-02

    Applicant: Tetracore, Inc.

    Inventor： William M. Nelson ,  Aymeric Randanne de Vazeille ,  Kyle Armantrout

    IPC: B01L7/00

    Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

    Methods for temperature-mediated nested polymerase chain reaction
    7.
    发明授权
    Methods for temperature-mediated nested polymerase chain reaction 有权

    公开(公告)号：US11713489B2

    公开(公告)日：2023-08-01

    申请号：US17080085

    申请日：2020-10-26

    Applicant: Tetracore, Inc.

    Inventor： William M. Nelson ,  Kyle Armantrout ,  Tracy Calvin Fecteau

    IPC: C12Q1/689 ,  C12Q1/6844 ,  C12Q1/70

    CPC classification number: C12Q1/689 ,  C12Q1/6844 ,  C12Q1/701 ,  C12Q1/6844 ,  C12Q2527/107 ,  C12Q2547/101 ,  C12Q2549/119 ,  C12Q2561/113 ,  C12Q2563/107

    Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles. During the second phase, fluorescence probes anneal to the template strands and are degraded by DNA polymerase to emit fluorescence signals for detection and/or quantification of the target nucleic acid. Methods for amplifying nucleic acid in accordance with the disclosure may be employed for nucleic acid amplification and detection in clinical and research settings.

Patent Agency Ranking