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    Method of monitoring gene expression
    1.
    发明申请
    Method of monitoring gene expression 审中-公开
    监测基因表达的方法

    公开(公告)号：US20050244822A1

    公开(公告)日：2005-11-03

    申请号：US10501039

    申请日：2003-01-09

    申请人： Tetsuro Kokubo ,  Masahiro Shirakawa ,  Jeremy Tame

    发明人： Tetsuro Kokubo ,  Masahiro Shirakawa ,  Jeremy Tame

    IPC分类号： G01R33/465 ,  C12N15/09 ,  C12Q1/68 ,  G01N24/08 ,  G01N33/15 ,  G01N33/483 ,  G01N33/50

    CPC分类号： C12Q1/6809 ,  C12Q1/6897 ,  G01N24/08 ,  G01R33/465 ,  C12Q2565/633

    摘要： The present invention provides a technique by which highly detailed gene expression profiling data for analyzing the gene function can be obtained safely and conveniently. It is a versatile non-destructive and high-resolution visualizing technique by which expression levels of genes can be monitored in real time, and which can be applied in vivo and to deep tissues. For instance, plasmids in which molecules that vary the NMR signal and can be quantified by PHM genes are incorporated downstream from promoters responding to the condition of medium, external stimuli and the like, are introduced into the cells. The level of polyphosphate in deep tissues and the like can then be quantified by 31P-NMR, allowing expression profiling of the promoter, both non-destructively and in real time.

    摘要翻译： 本发明提供了可以安全，方便地获得用于分析基因功能的高度详细的基因表达谱分析数据的技术。 它是一种多功能的非破坏性和高分辨率可视化技术，可以实时监测基因的表达水平，并且可以在体内和深层组织中应用。 例如，将其中改变NMR信号并且可以通过PHM基因定量的分子的质粒引入到响应于培养基，外部刺激等条件的启动子的下游引入细胞中。 深层组织中的多磷酸盐水平等可以通过31-P-NMR进行定量，从而可以非破坏性地和实时地进行启动子的表达谱分析。

    NOVEL MONOCLONAL ANTIBODY AND USE OF THE SAME
    3.
    发明申请
    NOVEL MONOCLONAL ANTIBODY AND USE OF THE SAME 审中-公开
    新型单克隆抗体及其用途

    公开(公告)号：US20100112601A1

    公开(公告)日：2010-05-06

    申请号：US12309998

    申请日：2007-08-07

    申请人： Masahiro Shirakawa ,  Hiroshi Inooka

    发明人： Masahiro Shirakawa ,  Hiroshi Inooka

    IPC分类号： G01N33/567 ,  C07K16/00

    CPC分类号： C07K16/28 ,  C07K2317/55 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56966 ,  G01N33/74 ,  G01N2333/726

    摘要： The present invention provides an anti-PAC1 monoclonal antibody capable of recognizing a PAC1 having a native structure, a PAC1 activity regulator (in particular, activity inhibitor) containing the antibody, a prophylactic/therapeutic agent for a disease associated with accentuation of a bioactivity of PAC1, containing the antibody, a diagnostic reagent for a disease associated with an abnormality of PAC1 activity, containing the antibody, and a screening method for a substance that regulates the expression of PAC1, using the antibody and a PAC1-expressing cell.

    摘要翻译： 本发明提供能够识别具有天然结构的PAC1的抗PAC1单克隆抗体，含有抗体的PAC1活性调节剂（特别是活性抑制剂），与增加生物活性相关的疾病的预防/治疗剂 含有抗体的PAC1，含有抗体的PAC1活性异常的疾病的诊断试剂，以及使用抗体和PAC1表达细胞调节PAC1的表达的物质的筛选方法。

    Magnetic resonance force microscope
    4.
    发明授权
    Magnetic resonance force microscope 失效
    磁共振力显微镜

    公开(公告)号：US07400144B2

    公开(公告)日：2008-07-15

    申请号：US11712840

    申请日：2007-03-01

    申请人： Shigenori Tsuji ,  Yohsuke Yoshinari ,  Masahiro Shirakawa ,  Tetsuro Kobubo

    发明人： Shigenori Tsuji ,  Yohsuke Yoshinari ,  Masahiro Shirakawa ,  Tetsuro Kobubo

    IPC分类号： G01V3/00 ,  G01B5/28

    CPC分类号： G01Q60/08 ,  G01Q60/52

    摘要： A magnetic resonance force microscope (MRFM) generator for producing an RF magnetic field uniformly over the whole of a sample. A cantilever with magnetic probe tip is self-excited. Under this condition, spins in the sample are controlled to produce a magnetic resonance force. A frequency demodulator measures the resonant frequency of the cantilever from the output detection signal from a cantilever displacement-measuring instrument based on the magnetic resonance force.

    摘要翻译： 一种用于在整个样品上均匀地产生RF磁场的磁共振力显微镜（MRFM）发生器。 具有磁性探针尖端的悬臂是自激的。 在这种条件下，控制样品中的自旋以产生磁共振力。 频率解调器基于磁共振力从悬臂位移测量仪器的输出检测信号测量悬臂的谐振频率。

    Magnetic resonance force microscope
    5.
    发明申请
    Magnetic resonance force microscope 失效
    磁共振力显微镜

    公开(公告)号：US20070216412A1

    公开(公告)日：2007-09-20

    申请号：US11712840

    申请日：2007-03-01

    申请人： Shigenori Tsuji ,  Yohsuke Yoshinari ,  Masahiro Shirakawa ,  Tetsuro Kobubo

    发明人： Shigenori Tsuji ,  Yohsuke Yoshinari ,  Masahiro Shirakawa ,  Tetsuro Kobubo

    IPC分类号： G01V3/00

    CPC分类号： G01Q60/08 ,  G01Q60/52

    摘要： A magnetic resonance force microscope (MRFM) generator for producing an RF magnetic field uniformly over the whole of a sample. A cantilever with magnetic probe tip is self-excited. Under this condition, spins in the sample are controlled to produce a magnetic resonance force. A frequency demodulator measures the resonant frequency of the cantilever from the output detection signal from a cantilever displacement-measuring instrument based on the magnetic resonance force.

    摘要翻译： 一种用于在整个样品上均匀地产生RF磁场的磁共振力显微镜（MRFM）发生器。 具有磁性探针尖端的悬臂是自激的。 在这种条件下，控制样品中的自旋以产生磁共振力。 频率解调器基于磁共振力从悬臂位移测量仪器的输出检测信号测量悬臂的谐振频率。

    Substrate probe for use in measuring enzyme activity
    8.
    发明授权
    Substrate probe for use in measuring enzyme activity 有权
    用于测量酶活性的底物探针

    公开(公告)号：US08481254B2

    公开(公告)日：2013-07-09

    申请号：US12675511

    申请日：2008-09-08

    申请人： Fumio Yamauchi ,  Kimihiro Yoshimura ,  Katsuaki Kuge ,  Tetsuya Yano ,  Masahiro Shirakawa ,  Yasuhiro Aoyama ,  Shinsuke Sando

    发明人： Fumio Yamauchi ,  Kimihiro Yoshimura ,  Katsuaki Kuge ,  Tetsuya Yano ,  Masahiro Shirakawa ,  Yasuhiro Aoyama ,  Shinsuke Sando

    IPC分类号： C12Q1/00

    CPC分类号： C12Q1/54 ,  C12Q1/32 ,  C12Q1/34 ,  G01N24/088 ,  G01R33/281 ,  G01R33/4633 ,  G01R33/465 ,  G01R33/483 ,  G01R33/5601 ,  Y02P20/582

    摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.

    摘要翻译： 能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。 通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为 至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在 活动。 或者，通过多维核共振成像方法进行酶活性的成像。

    Umbrella support attachment for bicycle
    9.
    发明授权
    Umbrella support attachment for bicycle 失效
    伞支撑附件为自行车

    公开(公告)号：US5961015A

    公开(公告)日：1999-10-05

    申请号：US903006

    申请日：1997-07-30

    申请人： Masahiro Shirakawa

    发明人： Masahiro Shirakawa

    IPC分类号： B62J11/00

    CPC分类号： B62J11/00 ,  Y10S224/915

    摘要： An umbrella support attachment for a bicycle is provided which comprises a distal portion support member including a fastening portion to be attached to a seat stay of the bicycle and a support portion for supporting a distal portion of the umbrella, and a proximal portion support member including a fastening portion to be attached to a frame bar of the bicycle and a clipping portion for supporting a proximal portion of the umbrella. The umbrella support attachment is effective to carry an elongate object such as an umbrella on a bicycle without interfering with the operation of the bicycle and smooth movement of movable components of the bicycle.

    摘要翻译： 提供了一种用于自行车的伞形支撑附件，其包括远侧部分支撑构件，所述远端部分支撑构件包括附接到自行车的座椅支撑件的紧固部分和用于支撑伞的远侧部分的支撑部分，以及近端部分支撑构件， 固定在自行车的框架杆上的紧固部分和用于支撑伞的近端部分的夹持部分。 伞形支撑附件有效地将诸如伞的细长物体携带在自行车上，而不会干扰自行车的操作并且使自行车的可移动部件平滑地移动。