公开(公告)号：US20190085324A1

公开(公告)日：2019-03-21

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US11214797B2

公开(公告)日：2022-01-04

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  G16B5/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US11209440B2

公开(公告)日：2021-12-28

申请号：US15245748

申请日：2016-08-24

申请人： THE BROAD INSTITUTE, INC. ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Vijay Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  A61K48/00 ,  C12N15/68 ,  C12N15/86 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20150368719A1

公开(公告)日：2015-12-24

申请号：US14846219

申请日：2015-09-04

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Alexander K. Shalek ,  Rahul Satija ,  Hongkun Park

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q2600/158 ,  G01N33/5047 ,  G01N33/6893 ,  G01N2800/24

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications.

摘要翻译： 本发明一般涉及用于鉴定调节，控制或以其他方式影响树突细胞（DC）反应的调节网络的组合物和方法，例如树突状细胞成熟，树突细胞抗病毒应答和/或树突状细胞炎症 响应以及利用调节，控制或以其他方式影响各种治疗和/或诊断适应症中的树突状细胞反应的调节网络的组合物和方法。

公开(公告)号：US11566279B2

公开(公告)日：2023-01-31

申请号：US16244058

申请日：2019-01-09

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Aviv Regev ,  Evan Zane Macosko ,  Steven Andrew McCarroll ,  Alexander K. Shalek ,  Anindita Basu ,  Christopher B. Ford ,  Hongkun Park ,  David A. Weitz

IPC分类号： C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12N15/10 ,  G06K19/06

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

公开(公告)号：US20180100201A1

公开(公告)日：2018-04-12

申请号：US15844601

申请日：2017-12-17

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  Dana-Farber Cancer Institute, Inc. ,  The General Hospital Corporation

发明人： Levi A. Garraway ,  Benjamin Izar ,  Sanjay Prakadan ,  Aviv Regev ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Mario Suva ,  Itay Tirosh ,  Andrew Venteicher ,  Marc H. Wadsworth II ,  Bradley Bernstein ,  Anuraag Parikh ,  Sidharth Puram

IPC分类号： C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158

摘要： This invention relates generally to compositions and methods for identifying genes and gene networks that respond to, modulate, control or otherwise influence tumors and tissues, including cells and cell types of the tumors and tissues, and malignant, microenvironmental, or immunologic states of the tumor cells and tissues. The invention also relates to methods of diagnosing, prognosing and/or staging of tumors, tissues and cells, and provides compositions and methods of modulating expression of genes and gene networks of tumors, tissues and cells, as well as methods of identifying, designing and selecting appropriate treatment regimens. The invention also relates to the modulation of complement activity to shift cellular immunity and obtain an effective therapeutic response.

公开(公告)号：US20200081017A1

公开(公告)日：2020-03-12

申请号：US16675398

申请日：2019-11-06

申请人： The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Vijay K. Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20190263912A1

公开(公告)日：2019-08-29

申请号：US16348911

申请日：2017-11-07

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The General Hospital Corporation

发明人： Adam Haber ,  Moshe Biton ,  Rebecca H. Herbst ,  Karthik Shekhar ,  Christopher Smillie ,  Orit Rozenblatt-Rosen ,  Ramnik Xavier ,  Aviv Regev ,  Jose Ordovas-Montanes ,  Alexander K. Shalek ,  Noga Rogel

IPC分类号： C07K16/28 ,  C07K14/47 ,  C07K14/52 ,  A61K35/17 ,  C07K14/71 ,  C12N5/0783 ,  G01N33/50 ,  C12N5/071 ,  A61P1/04 ,  A61P1/14 ,  G01N33/569 ,  A61P33/00 ,  A61P31/04

摘要： An atlas of intestinal epithelial cells, intestinal epithelial stem cells and intestinal immune cells identifies new cell populations, markers, networks, and responses to stimuli. Intestinal T cells drive intestinal epithelial cell differentiation and activity. Accordingly, disclosed are methods of modulating intestinal epithelial cell differentiation, maintenance and/or function, related methods for the treatment of disease, including IBD. Also disclosed are methods and kits for identifying cell types, their differentiation, homeostasis and activation.

公开(公告)号：US12105089B2

公开(公告)日：2024-10-01

申请号：US16632018

申请日：2018-07-17

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The General Hospital Corporation

发明人： Alexander K. Shalek ,  Christopher Smillie ,  Rebecca H. Herbst ,  Moshe Biton ,  Aviv Regev ,  Jose Ordovas-Montanes ,  Ramnik Xavier

IPC分类号： G01N33/569 ,  C12Q1/6883 ,  G01N33/68

CPC分类号： G01N33/56966 ,  C12Q1/6883 ,  G01N33/6893 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N2800/065 ,  G01N2800/52

摘要： The present invention provides for a human cell atlas of the colon from healthy and diseased subjects. The atlas was obtained by single sequencing of about 117,000 cells. The present invention discloses novel markers for cell types. Moreover, genes associated with disease are identified in the colon and colon specific cell types. The invention provides for diagnostic assays based on gene markers and cell composition, as well as target cell types that express genes associated with disease. Finally, disclosed are novel cell types and methods of quantitating, detecting and isolating the cell types.

公开(公告)号：US11597964B2

公开(公告)日：2023-03-07

申请号：US15453405

申请日：2017-03-08

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Aviv Regev ,  Evan Zane Macosko ,  Steven Andrew McCarroll ,  Alexander K. Shalek ,  Anindita Basu ,  Christopher B. Ford ,  Hongkun Park ,  David A. Weitz

IPC分类号： C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12N15/10 ,  G06K19/06

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.