公开(公告)号：US20170349950A1

公开(公告)日：2017-12-07

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N33/5023

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20190085324A1

公开(公告)日：2019-03-21

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US11427869B2

公开(公告)日：2022-08-30

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  G01N33/50

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US11214797B2

公开(公告)日：2022-01-04

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  G16B5/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US11209440B2

公开(公告)日：2021-12-28

申请号：US15245748

申请日：2016-08-24

申请人： THE BROAD INSTITUTE, INC. ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Vijay Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  A61K48/00 ,  C12N15/68 ,  C12N15/86 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20190285644A1

公开(公告)日：2019-09-19

申请号：US16352355

申请日：2019-03-13

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Jellert Gaublomme

IPC分类号： G01N33/68 ,  C12N15/10

摘要： The application relates to methods and systems for proteomics and spatial mapping of biomolecules using a next generation sequencing readout to decipher biomolecular and cellular interaction networks. Specifically, disclosed are antenna networks generated by conjugating DNA antennas to proteins. The antennas carry a unique antenna identifier (UAI) sequence that can provide spatial location of the network, as well as biomolecules by transfer of the UAI to reporter oligonucleotides associated with other antennas and biomolecules. The methods and systems are also applicable to single cells.

公开(公告)号：US11072816B2

公开(公告)日：2021-07-27

申请号：US15970791

申请日：2018-05-03

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jellert Gaublomme ,  Aviv Regev

IPC分类号： C12Q1/6818 ,  G01N33/68 ,  C12N15/115 ,  G01N33/543

摘要： The application relates to proteome analysis in single cells. Specifically, disclosed are high throughput methods of detecting proteins in single cells using barcoding, aptamers and single cell sequencing. Solid supports used in recording the cell-of-origin of target proteins and target proteins expressed in the cell-of-origin are disclosed. Additionally, methods of detecting proteins and mRNA in single cells are disclosed. Additionally, methods of detecting protein interactions are disclosed. Additionally, methods of detecting post translationally modified proteins in single cells are disclosed. The application also relates to solid supports or beads and methods of producing said solid supports or beads for use in the described methods.

公开(公告)号：US11092607B2

公开(公告)日：2021-08-17

申请号：US15771987

申请日：2016-10-27

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jellert Gaublomme ,  Aviv Regev

IPC分类号： G01N33/68 ,  G01N33/58 ,  C12Q1/6869 ,  C12Q1/6811 ,  C12Q1/6823 ,  C12Q1/683

摘要： The present invention relates to methods for high multiplex protein or cellular constituent analysis in single cells or single isolated aggregations of cellular constituents. The methods provide for embedding cells or isolated aggregations of cellular constituents in a hydrogel mesh and labeling of cellular constituents with labeling ligands linked to a nucleic acid tag. Cellular constituents can be determined using sequencing methods.

公开(公告)号：US20200081017A1

公开(公告)日：2020-03-12

申请号：US16675398

申请日：2019-11-06

申请人： The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Vijay K. Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US11332736B2

公开(公告)日：2022-05-17

申请号：US16770380

申请日：2018-12-07

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Orit Rozenblatt-Rosen ,  Jellert Gaublomme ,  Bo Li ,  Kathryn Geiger-Schuller ,  Pratiksha Thakore

IPC分类号： C12N15/10

摘要： The present invention provides methods and tools for analyzing genetic interactions. The subject matter disclosed herein is generally directed to single cell genomics and proteomics. In one embodiment provided is a method of cell and nuclei hashing using sample barcodes. In another embodiment are method of performing genomewide CRISPR perturbation screens.