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1.发明授权公开(公告)号：US11793870B2
公开(公告)日：2023-10-24
申请号：US17330545
申请日：2021-05-26
发明人： Teresa B. De Los Santos , Aida E. Rieder , Fayna C. Diaz-San Segundo , Anna Kloc , John R. Coleman , Steffen Mueller , Gisselle N. Medina
IPC分类号： A61K39/135 , C12N7/00 , A61K39/00
CPC分类号： A61K39/135 , C12N7/00 , A61K2039/5254 , A61K2039/552 , C12N2770/32121 , C12N2770/32134
摘要： The present disclosure describes deoptimized foot and mouth viruses and their use for prophylactic and therapeutic treatment of mammalian subjects. The recombinant viruses provided herein include alterations in several genomic regions as well as Differentiating Infected from Vaccinated Animals (DIVA) markers.
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公开(公告)号：US11813320B2
公开(公告)日：2023-11-14
申请号：US17176989
申请日：2021-02-16
申请人： LAWRENCE LIVERMORE NATIONAL SECURITY, LLC , THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE , THE UNIVERSITY OF CONNECTICUT
发明人： Aida E. Rieder , Teresa B. De Los Santos , Luis L. Rodriguez , Devendra Rai , Fayna C. Diaz-San Segundo , Paul D. Hoeprich
CPC分类号： A61K39/135 , A61K39/12 , A61P31/14 , C12N7/00 , C12Q1/70 , G01N33/53 , A61K2039/5254 , A61K2039/55555 , A61K2039/55566 , A61K2039/55572 , C12N2770/32122 , C12N2770/32134 , C12N2770/32141 , C12N2770/32162
摘要： Genetically engineered Foot and Mouth Disease Virus (FMDV) and related engineered proteins and polynucleotides, nanolipoprotein particles, compositions, methods and systems are described. The genetically engineered FMDV is modified by the strategic insertion of a protein tag into select regions of the FMDV genome which encode viral proteins that are exposed on the surface of the FMDV viral capsid. The inserted protein tag is displayed as a decoration or attachment on the viral capsid surface.
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3.发明授权Development of a marker foot and mouth disease virus vaccine candidate that is attenuated in the natural host 有权开发在天然宿主中减毒的标志物口蹄疫病毒疫苗候选物公开(公告)号：US09180179B1
公开(公告)日：2015-11-10
申请号：US14280984
申请日：2014-05-19
IPC分类号： G01N33/569 , A61K39/135 , C12N7/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/135 , A61K2039/5252 , A61K2039/5254 , A61K2039/552 , A61K2039/55566 , C12N7/00 , C12N2770/32121 , C12N2770/32122 , C12N2770/32134 , C12N2770/32151 , C12N2770/32162 , C12N2770/32163 , C12N2770/32171 , C12Q1/701 , G01N33/56983 , G01N2469/10
摘要： We have generated novel molecularly marked FMDV A24LL3DYR and A24LL3BPVKV3DYR vaccine candidates. The mutant viruses contain a deletion of the leader coding region (LL) rendering the virus attenuated in vivo and negative antigenic markers introduced in one or both of the viral non-structural 3Dpol and 3B proteins. The vaccine platform includes unique restriction endonuclease sites for easy swapping of capsid proteins for different FMDV subtypes and serotypes. The mutant viruses produced no signs of FMD and no shedding of virulent virus in cattle. No clinical signs of disease or fever were observed and no transmission to in-contact animals was detected in pigs inoculated with live A24LL3DYR. Cattle immunized with chemically inactivated vaccine candidates showed an efficacy comparable to a polyvalent commercial FMDV vaccine. These vaccine candidates used in conjunction with a cELISA provide a suitable target for DIVA companion tests.
摘要翻译： 我们生成了新型分子标记的FMDV A24LL3DYR和A24LL3BPVKV3DYR疫苗候选物。 突变体病毒包含导致体内减毒的前导编码区（LL）的缺失和引入病毒非结构3Dpol和3B蛋白之一或两者的阴性抗原标记。 疫苗平台包括独特的限制性内切核酸酶位点，可以方便地交换不同FMDV亚型和血清型的衣壳蛋白。 突变体病毒不产生FMD的迹象，并且不会在牛中脱落毒性病毒。 没有观察到疾病或发烧的临床症状，并且在接种活A24LL3DYR的猪中未检测到接触动物的传播。 用化学灭活疫苗候选者免疫的牛显示出与多价商业FMDV疫苗相当的功效。 与cELISA一起使用的这些疫苗候选者为DIVA伴侣测试提供了合适的目标。
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公开(公告)号：US10172933B2
公开(公告)日：2019-01-08
申请号：US15785875
申请日：2017-10-17
申请人： The United States of America, as represented by the Secretary of Agriculture , LOS ALAMOS NATIONAL SECURITY, LLC
IPC分类号： A61K39/135 , C12N7/00 , A61K39/12 , A61K39/00
摘要： Synthetic foot-and-mouth disease virus (FMDV) mosaic polypeptides, and nucleic acid molecules encoding the mosaic polypeptides, are described. The mosaic polypeptides have greater T-cell epitope coverage than naturally occurring FMDV polypeptides, and include common FMDV epitopes, but exclude rare FMDV epitopes. When included as part of an FMDV genome, the mosaic polypeptides permit virus replication and assembly into FMDV particles. The mosaic polypeptide and nucleic acid compositions can be used to elicit immune responses that provide protection against a broad range of serotype A FMDV strains.
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公开(公告)号：US20230149528A1
公开(公告)日：2023-05-18
申请号：US17889737
申请日：2022-08-17
申请人： The United States of America, as represented by the Secretary of Agriculture , Triad National Security, LLC , KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
发明人： Aida E. Rieder , KATHERINE M. PFLAUM , FAYNA C. DIAZ SAN SEGUNDO , WILLIAM M. FISCHER , TATJANA SITT
IPC分类号： A61K39/135 , A61P31/14
CPC分类号： A61K39/135 , A61P31/14 , A61K2039/552
摘要： Synthetic foot-and-mouth disease virus (FMDV) mosaic polypeptides, and nucleic acid molecules encoding the mosaic polypeptides, are described. When included as part of an FMDV genome, the mosaic polypeptides permit virus replication and assembly into FMDV particles. The mosaic polypeptide and nucleic acid compositions can be used to elicit immune responses that provide protection against a broad range of serotype O FMDV strains.
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公开(公告)号：US10953085B2
公开(公告)日：2021-03-23
申请号：US15590918
申请日：2017-05-09
申请人： LAWRENCE LIVERMORE NATIONAL SECURITY, LLC , THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE
发明人： Aida E. Rieder , Teresa B. De Los Santos , Luis L. Rodriguez , Devendra Rai , Fayna C. Diaz-San Segundo , Paul D. Hoeprich
摘要： Genetically engineered Foot and Mouth Disease Virus (FMDV) and related engineered proteins and polynucleotides, nanolipoprotein particles, compositions, methods and systems are described. The genetically engineered FMDV is modified by the strategic insertion of a protein tag into select regions of the FMDV genome which encode viral proteins that are exposed on the surface of the FMDV viral capsid. The inserted protein tag is displayed as a decoration or attachment on the viral capsid surface.
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