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公开(公告)号：US4179334A
公开(公告)日：1979-12-18
申请号：US715798
申请日：1976-08-19
IPC分类号： C12P7/64 , C12N1/00 , C12N9/20 , C12P9/00 , C12Q1/44 , C12Q1/61 , C12R1/72 , G01N33/92 , G01N31/14
CPC分类号： C12N9/20 , C12Q1/61 , Y10S435/822 , Y10S435/921
摘要： A novel process is described for hydrolyzing protein-bound triglycerides such as blood serum triglycerides comprising contacting sample containing protein-bound triglycerides with a compatible mixture of an enzyme preparation which demonstrates triglyceride hydrolase activity and, as an effector, a surfactant.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates triglyceride hydrolase activity and an effector which is a surfactant are also described, as are analytical elements comprising at least one layer which includes a hydrolysis composition which comprises such a compatible mixture of an enzyme preparation which demonstrates triglyceride hydrolase activity and a surfactant.
摘要翻译： 描述了一种用于水解蛋白质结合的甘油三酸酯例如血清甘油三酸酯的新方法，其包括使含有蛋白质结合的甘油三酸酯的样品与显示甘油三酯水解酶活性的酶制剂的相容混合物和作为效应剂的表面活性剂接触。 还描述了包含显示甘油三酯水解酶活性的酶制剂和作为表面活性剂的效应物的相容混合物的水解组合物，包括至少一层的分析元件包括水解组合物，所述水解组合物包含这样的相容混合物， 显示甘油三酯水解酶活性和表面活性剂。
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公开(公告)号：US4275151A
公开(公告)日：1981-06-23
申请号：US970498
申请日：1978-12-18
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , Y10S435/822 , Y10S435/921 , Y10S435/939
摘要： A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.
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公开(公告)号：US4275152A
公开(公告)日：1981-06-23
申请号：US62433
申请日：1979-07-30
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , Y10S435/822 , Y10S435/921 , Y10S435/939
摘要： A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.
摘要翻译： 描述了用于水解蛋白质结合的胆固醇酯的新方法，例如在血清中发现的。 该方法包括使包含蛋白质结合的胆固醇酯的样品与显示胆固醇酯水解酶活性的酶制剂的相容混合物和作为效应剂的表面活性剂接触，所述表面活性剂是包含小于约20个氧化乙烯的聚氧乙烯链的烷基苯氧基聚乙氧基乙醇 单位。 还公开了包含显示胆固醇酯水解酶活性的酶制剂和作为所述表面活性剂的效应物的相容混合物的水解组合物，以及包含至少一层包含这种水解组合物的分析元件。
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4.发明授权Process and composition for the quantification of glycerol ATP and triglycerides 失效用于定量甘油ATP和甘油三酸酯的方法和组成
公开(公告)号：US4241178A
公开(公告)日：1980-12-23
申请号：US867641
申请日：1978-01-06
摘要： A method is described for the assay of glycerol (as either free glycerol or a fatty acid ester of glycerol) in aqueous liquids such as blood serum. The method comprises the steps ofI: contacting in the presence of an electron acceptor (a) a sample to be assayed and (b) a novel reagent composition comprising1. optionally, a lipase which hydrolyzes triglycerides to glycerol;2. glycerol kinase;3. adenosine triphosphate;4. .alpha.-glycerophosphate oxidase to produce a detectable change in the presence of triglyceride or a general positive sample; andII: detecting the occurrence of said detectable change.The lipase is included when fatty acid esters of glycerol (i.e., triglycerides) are to be detected. Free glycerol from whatever source can be detected with a composition comprising 2-4 above. According to a preferred embodiment, the electron acceptor is oxygen and the reagent composition also includes a hydrogen peroxide indicator composition, i.e., reagents which interact with hydrogen peroxide to produce a detectable product. The method can be used for assay of ATP. The method and reagent composition for performing the disclosed assays can be incorporated into a matrix of absorbent material.
摘要翻译： 描述了在诸如血清的水性液体中测定甘油（作为甘油的游离甘油或脂肪酸酯）的方法。 该方法包括以下步骤：在电子受体（a）待测定的样品存在下接触，和（b）新的试剂组合物，其包含任选的将甘油三酯水解成甘油的脂肪酶; 甘油激酶; 三磷酸腺苷 4.在甘油三酸酯或一般阳性样品的存在下产生可检测的变化的α-甘油磷酸酯氧化酶; 和II：检测所述可检测变化的发生。 当要检测甘油的脂肪酸酯（即甘油三酯）时，包括脂肪酶。 可以用包含2-4以上的组合物来检测来自任何来源的游离甘油。 根据优选实施方案，电子受体是氧，试剂组合物还包括过氧化氢指示剂组合物，即与过氧化氢相互作用以产生可检测产物的试剂。 该方法可用于ATP的测定。 用于进行所公开测定法的方法和试剂组合物可以并入吸收材料的基质中。
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公开(公告)号：US4444886A
公开(公告)日：1984-04-24
申请号：US407213
申请日：1982-08-11
CPC分类号： C12N9/20 , Y10S435/839
摘要： An enzyme which catalyzes the hydrolysis of glycerol esters is disclosed. The enzyme is specific for alkyl esters wherein the alkyl group has from 1 to 4 carbon atoms inclusive. The enzyme is particularly useful in hydrolyzing a diacetyl glycerol ester. The enzyme is from the microorganism Bacillus subtilis ATCC No. 31954.
摘要翻译： 公开了一种催化甘油酯水解的酶。 该酶对烷基酯是特异性的，其中烷基含有1至4个碳原子。 该酶特别可用于水解二乙酰甘油酯。 该酶来自微生物枯草芽孢杆菌ATCC No.31954。
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公开(公告)号：USRE32016E
公开(公告)日：1985-10-29
申请号：US297675
申请日：1981-08-31
IPC分类号： C12N9/04 , C12Q1/26 , G01N33/487
CPC分类号： C12N9/0006 , C12Q1/26
摘要： Compositions and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.
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公开(公告)号：US4276377A
公开(公告)日：1981-06-30
申请号：US91218
申请日：1979-11-05
CPC分类号： C12N9/78 , C12Q1/34 , G01N2333/978 , Y10S435/85
摘要： A urease-free creatinine iminohydrolase enzyme preparation obtained from an aerobic soil microorganism. The enzyme of the preparation preferably has a molecular weight of from about 250,000 to 300,000; a maximum activity at a pH between 7 and 8 as measured at 37.degree. C.; a K.sub.m of about 3 to 5 mM for creatinine as measured at 37.degree. C., pH 7.5; and a specific activity for creatinine of at least about 1.0 unit per milligram of protein in the preparation as measured at 37.degree. C., pH 7.5. The preferred enzyme preparation is derived from the aerobic soil microorganism ATCC 31,546. Assay methods, compositions, and elements containing the aforementioned urease-free creatinine iminohydrolase for the determination of creatinine in an aqueous liquid are also disclosed.
摘要翻译： 从需氧土壤微生物获得的无脲酶肌酐亚氨基水解酶制剂。 制剂的酶优选具有约25万至30万的分子量; 在37℃下测量的pH在7和8之间的最大活性。 在37℃，pH7.5下测量的肌酸酐的Km为约3至5mM; 并且在37℃，pH7.5下测定，所述制剂中肌酸酐的比活性为至少约1.0单位/毫克蛋白质。 优选的酶制剂来自需氧土壤微生物ATCC 31,546。 还公开了含有上述无脲酶肌酐亚氨基水解酶的测定方法，组合物和元件，用于测定水性液体中的肌酐。
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公开(公告)号：US4374930A
公开(公告)日：1983-02-22
申请号：US312474
申请日：1981-10-19
申请人： Roy E. Snoke , Charles T. Goodhue
发明人： Roy E. Snoke , Charles T. Goodhue
CPC分类号： C12N9/0006 , Y10S435/815
摘要： A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of:(a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid;(b) separating the solution from the steroid; and(c) contacting the steroid with an eluting solution containing a surfactant at a concentration between 0.001 percent and 10 percent so as to recover the adsorbed cholesterol oxidase.In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microorganism growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution containing the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid which comprises the step of first suspending the steroid in a water-miscible organic solvent.
摘要翻译： 公开了一种纯化和回收胆固醇氧化酶的方法。 胆固醇氧化酶通过包括以下步骤的方法纯化：（a）将待纯化的胆固醇氧化酶的溶液与包含未结合的水不溶性类固醇（其是胆固醇氧化酶的非底物）的色谱组合物接触，以吸附胆固醇氧化酶 对类固醇; （b）将溶液与类固醇分离; 和（c）使类固醇与含有0.001％至10％浓度的表面活性剂的洗脱溶液接触，以回收吸附的胆固醇氧化酶。 在优选的实施方案中，含有待纯化的胆固醇氧化酶的溶液是具有胆固醇氧化酶活性的微生物生长培养基，并且在将溶液与类固醇接触的步骤之前，将溶液与离子交换色谱材料接触。 在另一个实施方案中，含有表面活性剂的洗脱溶液还含有至少约0.005摩尔浓度的金属盐。 还公开了一种形成包含未结合类固醇的组合物的填充床的方法，该方法包括首先将类固醇悬浮在水混溶性有机溶剂中的步骤。
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公开(公告)号：US4166763A
公开(公告)日：1979-09-04
申请号：US749546
申请日：1976-12-10
IPC分类号： C12N9/02 , C12Q1/26 , G01N33/487 , G01N33/543 , C07G7/02 , G01N31/14
CPC分类号： C12Q1/26 , Y10S435/805 , Y10S435/885
摘要： Compositons and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.
摘要翻译： 提供了包含基本上不含过氧化氢酶并优选由粪肠球菌ATCC 12755产生的乳酸氧化酶的复合物和多层分析元件用于乳酸或乳酸盐，特别是在血清中的定量分析。 乳酸氧化酶催化乳酸或乳酸盐还原为丙酮酸盐和过氧化氢，乳酸或乳酸盐的量通过检测产生的过氧化氢的量来确定。 优选地，使用过氧化物酶催化的检测系统使用比色法检测过氧化氢。
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公开(公告)号：US5418151A
公开(公告)日：1995-05-23
申请号：US926874
申请日：1992-08-07
CPC分类号： C12P19/02 , C12P19/14 , C12P19/44 , Y10S435/921 , Y10S435/931 , Y10S435/933
摘要： There is provided a regio-selective method for the resolution of carbohydrate monoester mixtures, by treating such mixtures with one or more selective enzymes. The resolution of such mixtures results in monoesters comprising significantly purer isolates of one desired isomer.
摘要翻译： 通过用一种或多种选择性酶处理这种混合物，提供了用于分解碳水化合物单酯混合物的区域选择性方法。 这种混合物的分辨率导致包含一种所需异构体的显着更纯的分离物的单酯。
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