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公开(公告)号：US4444886A
公开(公告)日：1984-04-24
申请号：US407213
申请日：1982-08-11
CPC分类号： C12N9/20 , Y10S435/839
摘要： An enzyme which catalyzes the hydrolysis of glycerol esters is disclosed. The enzyme is specific for alkyl esters wherein the alkyl group has from 1 to 4 carbon atoms inclusive. The enzyme is particularly useful in hydrolyzing a diacetyl glycerol ester. The enzyme is from the microorganism Bacillus subtilis ATCC No. 31954.
摘要翻译： 公开了一种催化甘油酯水解的酶。 该酶对烷基酯是特异性的，其中烷基含有1至4个碳原子。 该酶特别可用于水解二乙酰甘油酯。 该酶来自微生物枯草芽孢杆菌ATCC No.31954。
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公开(公告)号：US4275151A
公开(公告)日：1981-06-23
申请号：US970498
申请日：1978-12-18
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , Y10S435/822 , Y10S435/921 , Y10S435/939
摘要： A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.
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3.发明授权Process and composition for the quantification of glycerol ATP and triglycerides 失效用于定量甘油ATP和甘油三酸酯的方法和组成
公开(公告)号：US4241178A
公开(公告)日：1980-12-23
申请号：US867641
申请日：1978-01-06
摘要： A method is described for the assay of glycerol (as either free glycerol or a fatty acid ester of glycerol) in aqueous liquids such as blood serum. The method comprises the steps ofI: contacting in the presence of an electron acceptor (a) a sample to be assayed and (b) a novel reagent composition comprising1. optionally, a lipase which hydrolyzes triglycerides to glycerol;2. glycerol kinase;3. adenosine triphosphate;4. .alpha.-glycerophosphate oxidase to produce a detectable change in the presence of triglyceride or a general positive sample; andII: detecting the occurrence of said detectable change.The lipase is included when fatty acid esters of glycerol (i.e., triglycerides) are to be detected. Free glycerol from whatever source can be detected with a composition comprising 2-4 above. According to a preferred embodiment, the electron acceptor is oxygen and the reagent composition also includes a hydrogen peroxide indicator composition, i.e., reagents which interact with hydrogen peroxide to produce a detectable product. The method can be used for assay of ATP. The method and reagent composition for performing the disclosed assays can be incorporated into a matrix of absorbent material.
摘要翻译： 描述了在诸如血清的水性液体中测定甘油（作为甘油的游离甘油或脂肪酸酯）的方法。 该方法包括以下步骤：在电子受体（a）待测定的样品存在下接触，和（b）新的试剂组合物，其包含任选的将甘油三酯水解成甘油的脂肪酶; 甘油激酶; 三磷酸腺苷 4.在甘油三酸酯或一般阳性样品的存在下产生可检测的变化的α-甘油磷酸酯氧化酶; 和II：检测所述可检测变化的发生。 当要检测甘油的脂肪酸酯（即甘油三酯）时，包括脂肪酶。 可以用包含2-4以上的组合物来检测来自任何来源的游离甘油。 根据优选实施方案，电子受体是氧，试剂组合物还包括过氧化氢指示剂组合物，即与过氧化氢相互作用以产生可检测产物的试剂。 该方法可用于ATP的测定。 用于进行所公开测定法的方法和试剂组合物可以并入吸收材料的基质中。
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公开(公告)号：US4179334A
公开(公告)日：1979-12-18
申请号：US715798
申请日：1976-08-19
IPC分类号： C12P7/64 , C12N1/00 , C12N9/20 , C12P9/00 , C12Q1/44 , C12Q1/61 , C12R1/72 , G01N33/92 , G01N31/14
CPC分类号： C12N9/20 , C12Q1/61 , Y10S435/822 , Y10S435/921
摘要： A novel process is described for hydrolyzing protein-bound triglycerides such as blood serum triglycerides comprising contacting sample containing protein-bound triglycerides with a compatible mixture of an enzyme preparation which demonstrates triglyceride hydrolase activity and, as an effector, a surfactant.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates triglyceride hydrolase activity and an effector which is a surfactant are also described, as are analytical elements comprising at least one layer which includes a hydrolysis composition which comprises such a compatible mixture of an enzyme preparation which demonstrates triglyceride hydrolase activity and a surfactant.
摘要翻译： 描述了一种用于水解蛋白质结合的甘油三酸酯例如血清甘油三酸酯的新方法，其包括使含有蛋白质结合的甘油三酸酯的样品与显示甘油三酯水解酶活性的酶制剂的相容混合物和作为效应剂的表面活性剂接触。 还描述了包含显示甘油三酯水解酶活性的酶制剂和作为表面活性剂的效应物的相容混合物的水解组合物，包括至少一层的分析元件包括水解组合物，所述水解组合物包含这样的相容混合物， 显示甘油三酯水解酶活性和表面活性剂。
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公开(公告)号：USRE32016E
公开(公告)日：1985-10-29
申请号：US297675
申请日：1981-08-31
IPC分类号： C12N9/04 , C12Q1/26 , G01N33/487
CPC分类号： C12N9/0006 , C12Q1/26
摘要： Compositions and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.
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公开(公告)号：US4276377A
公开(公告)日：1981-06-30
申请号：US91218
申请日：1979-11-05
CPC分类号： C12N9/78 , C12Q1/34 , G01N2333/978 , Y10S435/85
摘要： A urease-free creatinine iminohydrolase enzyme preparation obtained from an aerobic soil microorganism. The enzyme of the preparation preferably has a molecular weight of from about 250,000 to 300,000; a maximum activity at a pH between 7 and 8 as measured at 37.degree. C.; a K.sub.m of about 3 to 5 mM for creatinine as measured at 37.degree. C., pH 7.5; and a specific activity for creatinine of at least about 1.0 unit per milligram of protein in the preparation as measured at 37.degree. C., pH 7.5. The preferred enzyme preparation is derived from the aerobic soil microorganism ATCC 31,546. Assay methods, compositions, and elements containing the aforementioned urease-free creatinine iminohydrolase for the determination of creatinine in an aqueous liquid are also disclosed.
摘要翻译： 从需氧土壤微生物获得的无脲酶肌酐亚氨基水解酶制剂。 制剂的酶优选具有约25万至30万的分子量; 在37℃下测量的pH在7和8之间的最大活性。 在37℃，pH7.5下测量的肌酸酐的Km为约3至5mM; 并且在37℃，pH7.5下测定，所述制剂中肌酸酐的比活性为至少约1.0单位/毫克蛋白质。 优选的酶制剂来自需氧土壤微生物ATCC 31,546。 还公开了含有上述无脲酶肌酐亚氨基水解酶的测定方法，组合物和元件，用于测定水性液体中的肌酐。
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公开(公告)号：US4275152A
公开(公告)日：1981-06-23
申请号：US62433
申请日：1979-07-30
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , Y10S435/822 , Y10S435/921 , Y10S435/939
摘要： A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units.Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.
摘要翻译： 描述了用于水解蛋白质结合的胆固醇酯的新方法，例如在血清中发现的。 该方法包括使包含蛋白质结合的胆固醇酯的样品与显示胆固醇酯水解酶活性的酶制剂的相容混合物和作为效应剂的表面活性剂接触，所述表面活性剂是包含小于约20个氧化乙烯的聚氧乙烯链的烷基苯氧基聚乙氧基乙醇 单位。 还公开了包含显示胆固醇酯水解酶活性的酶制剂和作为所述表面活性剂的效应物的相容混合物的水解组合物，以及包含至少一层包含这种水解组合物的分析元件。
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公开(公告)号：US4275166A
公开(公告)日：1981-06-23
申请号：US91217
申请日：1979-11-05
CPC分类号： C12N9/00 , C12N9/78 , Y10S435/816 , Y10S435/85
摘要： A process for the recovery of an intracellular enzyme from an aerobic soil microorganism is disclosed. The recovery method is carried out by(a) forming an aqueous suspension of microbial cells containing the desired intracellular enzyme,(b) disrupting the microbial cells in the suspension to release the enzyme from the cells, and(c) before, during, or after step (b) and prior to removal of disrupted microbial cells and other cellular components, introducing a water-miscible organic solvent into the suspension to form a mixture of the organic solvent and the enzyme-containing suspension.The desired enzyme is retained in the liquid phase of the mixture formed in step (c) while undesired cellular components such as other microbial cell proteins precipitate therefrom.
摘要翻译： 公开了从需氧土壤微生物中回收细胞内酶的方法。 回收方法是通过（a）形成含有所需细胞内酶的微生物细胞的水性悬浮液，（b）破坏悬浮液中的微生物细胞以从细胞释放酶，和（c）在细胞内， 在步骤（b）之后并且在除去破坏的微生物细胞和其它细胞组分之前，将水混溶性有机溶剂引入悬浮液中以形成有机溶剂和含酶悬浮液的混合物。 期望的酶保留在步骤（c）中形成的混合物的液相中，而不希望的细胞组分如其它微生物细胞蛋白从其中沉淀出来。
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公开(公告)号：US4316954A
公开(公告)日：1982-02-23
申请号：US141748
申请日：1980-04-18
申请人： Roy E. Snoke , Theodore W. Esders
发明人： Roy E. Snoke , Theodore W. Esders
CPC分类号： C12Q1/26 , C12Q1/34 , C12Q1/527 , G01N2333/988 , Y10S435/805
摘要： A composition and element for assaying a neuraminic acid comprises a neuraminic acid aldolase, pyruvate oxidase and an electron acceptor. A sample containing a neuraminic acid can be contacted with the composition and the product detected spectrophotometrically.
摘要翻译： 用于测定神经氨酸的组合物和元件包括神经氨酸醛缩酶，丙酮酸氧化酶和电子受体。 可以将含有神经氨酸的样品与组合物接触，并用分光光度法检测产物。
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10.发明授权Thermostable polymerase specific antibody-containing DNA amplification composition and kit 失效耐热聚合酶特异性抗体的DNA扩增组合物和试剂盒
公开(公告)号：US5587287A
公开(公告)日：1996-12-24
申请号：US224218
申请日：1994-04-07
申请人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
发明人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
IPC分类号： C12N9/98 , C07K16/40 , C12N9/12 , C12N15/09 , C12P21/08 , C12Q1/68 , C12R1/91 , G01N33/53 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6804 , C07K16/40 , C12N9/1252 , C12Q1/6848
摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。
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