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13 条记录 (耗时 0.025 秒)

    Patterning Hydrogels
    5.
    发明申请
    Patterning Hydrogels 审中-公开
    图案水凝胶

    公开(公告)号：US20110053270A1

    公开(公告)日：2011-03-03

    申请号：US12862967

    申请日：2010-08-25

    申请人： Theresa Chang ,  Robert Randall Hancock, JR. ,  Trista Nicole Hesch ,  Michael Lesley Sorensen ,  Ruchirej Yongsunthon

    发明人： Theresa Chang ,  Robert Randall Hancock, JR. ,  Trista Nicole Hesch ,  Michael Lesley Sorensen ,  Ruchirej Yongsunthon

    IPC分类号： B05D3/06 ,  C12N5/00

    CPC分类号： C12N5/0068 ,  C12N2533/78 ,  C12N2535/00 ,  G03F7/095 ,  G03F7/26

    摘要： A method for forming a pattern-coated substrate includes disposing a composition comprising a polysaccharide-based polymer on a substrate to generate a coated substrate. The polysaccharide-based polymer composition is substantially free of cross-linking monomers. The method further includes exposing a portion of the coated substrate to a first dose of UV radiation to induce crosslinking of the polysaccharide-based polymer, wherein a portion of the substrate is shielded from the ionizing radiation. The UV exposed coated substrate may be washed or hydrated to remove uncross-linked polysaccharide-based polymer.

    摘要翻译： 形成图案涂布基板的方法包括在基材上设置包含多糖类聚合物的组合物以产生涂布的基材。 多糖类聚合物组合物基本上不含交联单体。 该方法还包括将涂覆的基底的一部分暴露于第一剂量的UV辐射以诱导多糖类聚合物的交联，其中基底的一部分与电离辐射屏蔽。 紫外线曝光的涂覆的基底可以被洗涤或水合以除去未交联的多糖基聚合物。

    CROSS-LINKED GUMS FOR HEPATOCYTE CULTURE
    6.
    发明申请
    CROSS-LINKED GUMS FOR HEPATOCYTE CULTURE 审中-公开
    HEPATOCYTE文化的交叉关系

    公开(公告)号：US20100190255A1

    公开(公告)日：2010-07-29

    申请号：US12500088

    申请日：2009-07-09

    申请人： Theresa Chang ,  Wageesha Senaratne ,  Lung-Ming Wu

    发明人： Theresa Chang ,  Wageesha Senaratne ,  Lung-Ming Wu

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N5/067 ,  C12N2533/70

    摘要： This disclosure relates to cell culture surfaces derived from or contain gums including naturally occurring gums, plant gums, galactomannan gums or derivatives thereof including carboxyalkyl guar gum. Even more particularly, the disclosure relates to chemically or physically cross-linked modified gums where the gum surfaces are tuned to provide cell culture surfaces with physical and chemical characteristics particularly suited for hepatocyte culture. The disclosure also relates to articles of manufacture (e.g., cell culture vessels and labware) having such matrices, methods of making and providing the matrices to cell culture surfaces, and methods of using cell culture vessels having such matrices.

    摘要翻译： 本公开涉及源自或含有牙龈的细胞培养物表面，包括天然存在的树胶，植物胶，半乳甘露聚糖胶或其衍生物，包括羧烷基瓜耳胶。 更具体地，本公开涉及化学或物理交联的改性胶，其中胶表面被调谐以提供特别适合于肝细胞培养的物理和化学特性的细胞培养物表面。 本公开还涉及具有这种基质的制品（例如，细胞培养容器和实验器皿），制备和提供基质到细胞培养物表面的方法，以及使用具有这种基质的细胞培养容器的方法。

    LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF
    8.
    发明申请
    LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF 有权
    LAMININ-ENTACTIN复合物和细胞培养物品及其方法

    公开(公告)号：US20110306136A1

    公开(公告)日：2011-12-15

    申请号：US13111234

    申请日：2011-05-19

    申请人： Theresa Chang ,  Jin Liu ,  Odessa Natalie Petzold ,  Ruchirej Yongsunthon

    发明人： Theresa Chang ,  Jin Liu ,  Odessa Natalie Petzold ,  Ruchirej Yongsunthon

    IPC分类号： C12N5/071 ,  C12M3/00

    CPC分类号： C12N5/0068 ,  C12M25/00 ,  C12N2533/50 ,  C12N2533/52

    摘要： A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    摘要翻译： 一种细胞培养系统，包括：底物，底物涂层，一个或多个活细胞和覆盖源。 底物涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白•内酰胺酶复合物。 或者，基底涂层和覆盖层可以分别是层粘连蛋白·内含子复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。

    Laminin-entactin complex and cell culture article and methods thereof
    10.
    发明授权
    Laminin-entactin complex and cell culture article and methods thereof 有权
    层粘蛋白 - 内含蛋白复合物和细胞培养物及其方法

    公开(公告)号：US08513015B2

    公开(公告)日：2013-08-20

    申请号：US13111234

    申请日：2011-05-19

    申请人： Theresa Chang ,  Jin Liu ,  Odessa Natalie Petzold ,  Ruchirej Yongsunthon

    发明人： Theresa Chang ,  Jin Liu ,  Odessa Natalie Petzold ,  Ruchirej Yongsunthon

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0068 ,  C12M25/00 ,  C12N2533/50 ,  C12N2533/52

    摘要： A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    摘要翻译： 一种细胞培养系统，包括：底物，底物涂层，一个或多个活细胞和覆盖源。 基底涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白复合物。 或者，基底涂层和覆盖层可以分别是层粘连蛋白复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。