公开(公告)号：US06217731B1

公开(公告)日：2001-04-17

申请号：US09176106

申请日：1998-10-21

申请人： Thomas E. Kane ,  Robert Eugene Fields, III

发明人： Thomas E. Kane ,  Robert Eugene Fields, III

IPC分类号： G01N2726

CPC分类号： G01N27/44782

摘要： An automated parallel electrophoresis system including a plurality of capillaries configured to accommodate samples during an electrophoresis run which includes a plurality of phases. The system also includes a control circuit coupled to the capillaries and configured to perform one of monitoring and regulating the capillaries, a display monitor and a computer processor coupled to the control circuit and the display monitor. The computer processor includes a input/output (I/O) interface configured to communicate with the control circuit and a first computer memory storing a display program which displays a graphical user interface on the display monitor. In addition, a method of monitoring and regulating an electrophoresis run performed on a parallel capillary electrophoresis system which comprises a plurality of capillaries configured to accommodate samples during an electrophoresis run. The method includes the steps of making electrical measurements on the capillaries during the electrophoresis run and displaying the measurements on a display screen.

摘要翻译： 一种自动平行电泳系统，包括多个毛细管，其被配置为在包括多个相的电泳运行期间容纳样品。 该系统还包括耦合到毛细管并被配置为执行监测和调节毛细管之一的控制电路，耦合到控制电路和显示监视器的显示监视器和计算机处理器。 计算机处理器包括被配置为与控制电路通信的输入/输出（I / O）接口和存储在显示监视器上显示图形用户界面的显示程序的第一计算机存储器。 另外，一种在并行毛细管电泳系统上执行的监测和调节电泳运行的方法，该方法包括多个毛细管，配置成在电泳过程中容纳样品。 该方法包括在电泳运行期间对毛细管进行电测量并在显示屏上显示测量的步骤。

公开(公告)号：US06982029B2

公开(公告)日：2006-01-03

申请号：US09849239

申请日：2001-05-07

申请人： Walter R. McVey ,  Thomas E. Kane

发明人： Walter R. McVey ,  Thomas E. Kane

IPC分类号： B01D59/42 ,  C07K1/26

CPC分类号： G01N27/44721 ,  G01N27/44726

摘要： An electrophoretic system having a plurality of separation lanes is provided with an automatic color calibration feature in which each lane is separately calibrated. For each lane, a dye matrix standard is created using reference fragments which migrate either before, or after, the sample fragments being electrophoresced, during the same electrophoresis run. These dye standards are detected automatically for the purpose of determining coefficients used to identify sample fragments mixed together with the reference fragments. This allows for calibrating the dye standard matrix for each of a plurality of lanes each time the electrophoresis system is used.

公开(公告)号：US20100282077A1

公开(公告)日：2010-11-11

申请号：US12660241

申请日：2010-02-23

申请人： Brian A. Jones ,  Thomas E. Kane

发明人： Brian A. Jones ,  Thomas E. Kane

IPC分类号： B44F1/08 ,  B01D53/02

CPC分类号： G01N30/12

摘要： A gas chromatography inlet liner comprises a glass tube formed by a glass wall, the glass wall having pore channels formed therein, and an indicator present in the pore channels in at least part of the inlet liner. The indicator may be any element, compound, dopant, or mixture additive that modifies color or transparency of the inlet liner.

摘要翻译： 气相色谱入口衬套包括由玻璃壁形成的玻璃管，其中形成有孔隙通道的玻璃壁和存在于入口衬里的至少一部分中的孔通道中的指示器。 指示剂可以是改变入口衬里的颜色或透明度的任何元素，化合物，掺杂剂或混合物添加剂。

公开(公告)号：US20090090630A1

公开(公告)日：2009-04-09

申请号：US12290087

申请日：2008-10-27

申请人： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

发明人： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

IPC分类号： G01N27/453

CPC分类号： C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

摘要： An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresed into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

摘要翻译： 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀门，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US07435545B2

公开(公告)日：2008-10-14

申请号：US11103356

申请日：2005-04-11

申请人： Zhaowei Liu ,  Thomas E. Kane

发明人： Zhaowei Liu ,  Thomas E. Kane

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q2565/125 ,  C12Q2565/102 ,  C12Q2545/101

摘要： According to a method of determining a size of a sample polynucleotide, a sample polynucleotide is subjected to electrophoresis in the presence of a fluorescent compound having a first fluorescence spectrum. Detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide. One or more size standards are also subjected to electrophoresis, optionally in the presence of the sample polynucleotide. If more than one size standard is used, the different size standards typically have different mobilities. The size standards are generally essentially or completely free of polynucleotides. Migration coordinates, e.g., migration times, of the sample polynucleotide and size standard(s) are determined. A size of the sample polynucleotide can be determined using the migration coordinate of the sample polynucleotide and the migration coordinate(s) of the size standard(s).

摘要翻译： 根据测定样品多核苷酸的大小的方法，在具有第一荧光光谱的荧光化合物的存在下对样品多核苷酸进行电泳。 第一荧光光谱的光的检测指示样品多核苷酸的存在。 也可以在样品多核苷酸的存在下，一个或多个尺寸标准品进行电泳。 如果使用多于一个尺寸的标准，则不同尺寸的标准通常具有不同的移动性。 大小标准通常基本上或完全不含多核苷酸。 测定样品多核苷酸的迁移坐标，例如迁移时间和大小标准。 可以使用样品多核苷酸的迁移坐标和尺寸标准的迁移坐标来确定样品多核苷酸的大小。

公开(公告)号：US5916428A

公开(公告)日：1999-06-29

申请号：US110877

申请日：1998-07-07

申请人： Thomas E. Kane ,  Qingbo China Li ,  John R. Kernan

发明人： Thomas E. Kane ,  Qingbo China Li ,  John R. Kernan

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The capillary cartridge has two embodiments. In one embodiment, the second ends of the capillary tubes are also arranged is such an array. In a second embodiment, the second ends communicate with an interior cavity of pressure cell from which solutions, gels and the like may be introduced. The pressure cell allows for applying high pressure to clean out the capillary tubes. An apparatus for performing automated capillary gel electrophoresis using such a cartridge is also disclosed.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 毛细管盒具有两个实施例。 在一个实施例中，毛细管的第二端也布置为这样的阵列。 在第二实施例中，第二端与压力池的内部空腔连通，可以引入溶液，凝胶等。 压力池允许施加高压以清洁毛细管。 还公开了使用这种盒进行自动毛细管凝胶电泳的装置。

公开(公告)号：US08438933B2

公开(公告)日：2013-05-14

申请号：US12460246

申请日：2009-07-15

申请人： William D. Grove ,  Brandon A. Tarr ,  Gerald W. Johnston ,  Thomas E. Kane

发明人： William D. Grove ,  Brandon A. Tarr ,  Gerald W. Johnston ,  Thomas E. Kane

IPC分类号： G01F1/00

CPC分类号： G01F1/36 ,  G01F1/363

摘要： An acoustic displacement flowmeter for determining flow rate of a fluid has a fluid flow line defining a flow path for the fluid. The fluid flow line has an inlet and an outlet. A controllable valve is positioned along the fluid flow line for opening and closing the flow path as desired, and an acoustic displacement transducer having a fluid port is coupled to the fluid flow line upstream from the controllable valve. The outlet of the fluid flow line extends through a wall isolating fluid exhausted from the outlet of the fluid flow line from electrical and/or mechanical components residing inside the flowmeter, thereby preventing the exhausted fluid, if explosive, from contacting such electrical and/or mechanical components which may act as potential ignition sources. Preferably, a manifold having a rigid body is provided in which the fluid flow line is formed. An acoustic displacement transducer (ADT) chamber wall is formed on the manifold body, and has an ADT chamber inlet port formed therein, and the fluid flow line has a branched portion connecting the fluid flow line to the ADT chamber inlet port. The outlet of the fluid flow line extends through a wall formed in the manifold body to isolate fluid exhausted from the outlet of the fluid flow line from electrical and/or mechanical components residing inside the flowmeter.

摘要翻译： 用于确定流体流速的声位移流量计具有限定流体流动路径的流体流动线。 流体流动管线具有入口和出口。 沿着流体流动管线定位可控阀，以根据需要打开和关闭流路，并且具有流体端口的声位移传感器联接到可控阀上游的流体流动管线。 流体流动管线的出口延伸通过流体流动管线出口从位于流量计内部的电气和/或机械部件排出的壁的隔离壁延伸，从而防止排出的流体（如果有爆炸性物质）接触这种电气和/或 可能作为潜在点火源的机械部件。 优选地，提供具有刚性体的歧管，其中形成流体流动线。 声学位移传感器（ADT）室壁形成在歧管主体上，并具有形成在其中的ADT室入口，流体流动线具有将流体流动线连接到ADT室入口的分支部分。 流体流动管线的出口延伸穿过形成在歧管体中的壁，以将从流体流动管线的出口排出的流体与驻留在流量计内部的电气和/或机械部件分离。

公开(公告)号：US06547943B1

公开(公告)日：2003-04-15

申请号：US09577479

申请日：2000-05-25

申请人： Thomas E. Kane ,  Li Qingbo

发明人： Thomas E. Kane ,  Li Qingbo

IPC分类号： G01N2726

CPC分类号： G01N27/44743 ,  G01N27/44782

摘要： The present invention provides capillary an electrophoresis system and method for multiple simultaneous analysis of a sample. The system and method of the invention allow the ends of a plurality of capillaries to simultaneously contact a single body of electrophoresis sample contained in a compartment of a container, such as a sample contained in a well of a micro-titer tray.

摘要翻译： 本发明提供毛细管电泳系统和用于多次同时分析样品的方法。 本发明的系统和方法允许多个毛细管的端部同时接触包含在容器隔室中的单体电泳样品，例如包含在微量滴定盘的孔中的样品。

公开(公告)号：US06375819B1

公开(公告)日：2002-04-23

申请号：US09373909

申请日：1999-08-13

申请人： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

发明人： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

IPC分类号： G01N2726

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US5885430A

公开(公告)日：1999-03-23

申请号：US726877

申请日：1996-10-04

申请人： John R. Kernan ,  Thomas E. Kane

发明人： John R. Kernan ,  Thomas E. Kane

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The capillary cartridge has two embodiments. In one embodiment, the second ends of the capillary tubes are also arranged is such an array. In a second embodiment, the second ends communicate with an interior cavity of pressure cell from which solutions, gels and the like may be introduced. The pressure cell allows for applying high pressure to clean out the capillary tubes. An apparatus for performing automated capillary gel electrophoresis using such a cartridge is also disclosed.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 毛细管盒具有两个实施例。 在一个实施例中，毛细管的第二端也布置为这样的阵列。 在第二实施例中，第二端与压力池的内部空腔连通，可以引入溶液，凝胶等。 压力池允许施加高压以清洁毛细管。 还公开了使用这种盒进行自动毛细管凝胶电泳的装置。