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公开(公告)号：US08771615B2
公开(公告)日：2014-07-08
申请号：US12736337
申请日：2009-03-03
申请人： Tomoyuki Shibata , Natsuki Masuya , Yoshiaki Seto , Shingo Saito , Yasuyoshi Mori , Yutaka Kubota , Hidetoshi Kanda , Tsugunori Notomi , Teijirou Kanada , Youichi Takano , Yasuyuki Takeuchi , Akira Onishi
发明人： Tomoyuki Shibata , Natsuki Masuya , Yoshiaki Seto , Shingo Saito , Yasuyoshi Mori , Yutaka Kubota , Hidetoshi Kanda , Tsugunori Notomi , Teijirou Kanada , Youichi Takano , Yasuyuki Takeuchi , Akira Onishi
IPC分类号： B01L3/14
CPC分类号： B65D47/06 , B65D51/222 , B65D51/226 , B65D51/227 , B65D51/285 , B65D2251/0025 , B65D2251/0093 , B65D2251/0096 , Y10T436/2575
摘要： A compound container has a container main body forming a first accommodation chamber, an auxiliary container forming a second accommodation chamber, a mounting part on which the auxiliary container is mounted in the container main body, a cutting part which cuts a second accommodation chamber partition wall which partitions a part of the second accommodation chamber formed in the auxiliary container, a contents outlet port in the container main body, and an opening pouring element mounted on the contents outlet port and having another cutting part which cuts a first accommodation chamber partition wall which partitions a part of the first accommodation chamber formed in the container main body.
摘要翻译： 复合容器具有形成第一容纳室的容器主体，形成第二容纳室的辅助容器，辅助容器安装在容器主体中的安装部，切割第二容纳室隔壁 其分隔形成在辅助容器中的第二容纳室的一部分，容器主体中的内容物出口和安装在内容物出口上的开口浇注元件，并具有另一个切割部分，该切割部分切割第一容纳室分隔壁， 分隔形成在容器主体中的第一容纳室的一部分。
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公开(公告)号：US20110052459A1
公开(公告)日：2011-03-03
申请号：US12736337
申请日：2009-03-03
申请人： Tomoyuki Shibata , Natsuki Masuya , Yoshiaki Seto , Shingo Saito , Yasuyoshi Mori , Yutaka Kubota , Hidetoshi Kanda , Tsugunori Notomi , Teijirou Kanada , Youichi Takano , Yasuyuki Takeuchi , Akira Onishi
发明人： Tomoyuki Shibata , Natsuki Masuya , Yoshiaki Seto , Shingo Saito , Yasuyoshi Mori , Yutaka Kubota , Hidetoshi Kanda , Tsugunori Notomi , Teijirou Kanada , Youichi Takano , Yasuyuki Takeuchi , Akira Onishi
CPC分类号： B65D47/06 , B65D51/222 , B65D51/226 , B65D51/227 , B65D51/285 , B65D2251/0025 , B65D2251/0093 , B65D2251/0096 , Y10T436/2575
摘要： A compound container which is comprised of a container main body 2 forming a first accommodation chamber 20 and an auxiliary container 3 forming a second accommodation chamber 30, wherein a mounting part 22 on which the auxiliary container 3 is mounted is provided in the container main body 2 and a cutting part 221 which cuts a second accommodation chamber partition wall 30a which partitions part of the second accommodation chamber 30 formed in the auxiliary container 3 is formed, and when the auxiliary container 3 is mounted on the container main body 2, the second accommodation chamber partition wall 30a is cut, whereby the first accommodation chamber 20 and the second accommodation chamber 30 are intercommunicated. As a result, if contents which are needed to be heat-treated are heated while keeping the state where the contents are sealed within the container, the contents which are sealed in a plurality of accommodation chambers formed in the container can be mixed while keeping the contents being isolated from the outside environment by effectively preventing the contents to be heated from leaking outside as well as by preventing deterioration of sealing performance.
摘要翻译： 复合容器，其由形成第一容纳室20的容器主体2和形成第二容纳室30的辅助容器3构成，其中安装有辅助容器3的安装部22设置在容器主体 形成有分隔形成在辅助容器3中的第二容纳室30的一部分的第二容纳室分隔壁30a的切割部221，当辅助容器3安装在容器主体2上时， 收纳室分隔壁30a被切断，由此第一容纳室20和第二容纳室30相互连通。 结果，如果在保持内容物被密封在容器内的状态的同时加热需要热处理的内容物，则密封在形成在容器中的多个容纳室中的内容物可以混合，同时保持 内容物通过有效地防止内容物被加热泄漏到外部以及防止密封性能的劣化而与外部环境隔离。
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3.发明授权Method and kit for preparation of sample for use in nucleic acid amplification 有权制备用于核酸扩增的样品的方法和试剂盒公开(公告)号：US09347055B2
公开(公告)日：2016-05-24
申请号：US12741322
申请日：2008-11-05
CPC分类号： C12N15/1006
摘要： The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.
摘要翻译： 本发明的目的是去除能够抑制生物样品和核酸提取物的核酸扩增的物质，并且允许方便和准确地评估靶核酸的存在或不存在或生物样品中靶基因的表达水平， 通过使用酶的核酸扩增方法。 本发明提供了一种制备用于核酸扩增的样品的方法，用于生物样品中的核酸扩增，该方法包括提取步骤，其中将核酸提取试剂加入到生物样品中至 获得核酸提取物和纯化步骤，其中使核酸提取物与沸石接触以获得适于核酸扩增的核酸扩增样品，以允许以高灵敏度进行核酸检测。
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公开(公告)号：US07175985B1
公开(公告)日：2007-02-13
申请号：US10129637
申请日：2002-08-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6827 , C12Q1/6858 , Y02A50/58 , C12Q2537/1373 , C12Q2531/101 , C12Q2531/119 , C12Q2525/161
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
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公开(公告)号：US07638280B2
公开(公告)日：2009-12-29
申请号：US11615742
申请日：2006-12-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y02A50/58
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
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公开(公告)号：US20070238113A1
公开(公告)日：2007-10-11
申请号：US11615742
申请日：2006-12-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y02A50/58
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
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7.发明申请METHOD AND KIT FOR PREPARATION OF SAMPLE FOR USE IN NUCLEIC ACID AMPLIFICATION 有权制备用于核酸扩增的样品的方法和试剂盒公开(公告)号：US20110015379A1
公开(公告)日：2011-01-20
申请号：US12741322
申请日：2008-11-05
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.
摘要翻译： 本发明的目的是去除能够抑制生物样品和核酸提取物的核酸扩增的物质，并且允许方便和准确地评估靶核酸的存在或不存在或生物样品中靶基因的表达水平， 通过使用酶的核酸扩增方法。 本发明提供了一种制备用于核酸扩增的样品的方法，用于生物样品中的核酸扩增，该方法包括提取步骤，其中将核酸提取试剂加入到生物样品中至 获得核酸提取物和纯化步骤，其中使核酸提取物与沸石接触以获得适于核酸扩增的核酸扩增样品，以允许以高灵敏度进行核酸检测。
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8.发明授权公开(公告)号：US08557523B2
公开(公告)日：2013-10-15
申请号：US12738413
申请日：2008-10-17
CPC分类号： C12Q1/6876 , C12Q1/6851 , C12Q2561/113 , C12Q2527/143 , C12Q2527/113 , C12Q2545/101 , C12Q2537/149 , C12Q2531/119
摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。
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9.发明申请METHOD OF NUCLEIC ACID AMPLIFICATION AND MEASURING REAGENT AND REAGENT KIT THEREFOR 失效核酸扩增方法及其测试试剂及其试剂盒公开(公告)号：US20100233715A1
公开(公告)日：2010-09-16
申请号：US12738413
申请日：2008-10-17
CPC分类号： C12Q1/6876 , C12Q1/6851 , C12Q2561/113 , C12Q2527/143 , C12Q2527/113 , C12Q2545/101 , C12Q2537/149 , C12Q2531/119
摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。
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公开(公告)号：US09909168B2
公开(公告)日：2018-03-06
申请号：US12331196
申请日：2008-12-09
申请人： Tsugunori Notomi , Tetsu Hase
发明人： Tsugunori Notomi , Tetsu Hase
CPC分类号： C12Q1/6853 , C12N15/10 , C12Q1/6844 , C12Q2525/161 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119 , C12Q2525/301
摘要： The present invention relates to an oligonucleotide having a novel structure and a method of synthesizing nucleic acid by using the same as a primer. This oligonucleotide is provided at the 5′-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the present invention provides a method of synthesizing highly specific nucleic acid on the basis of this method of synthesizing nucleic acid.
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