公开(公告)号：US08441080B2

公开(公告)日：2013-05-14

申请号：US12793647

申请日：2010-06-03

申请人： Tung-Ming Pan ,  Min-Hsien Wu ,  Ming-De Huang ,  Chao-Sung Lai

发明人： Tung-Ming Pan ,  Min-Hsien Wu ,  Ming-De Huang ,  Chao-Sung Lai

IPC分类号： H01L27/14

CPC分类号： A61B5/14539 ,  C12Q1/001 ,  G01N27/4145

摘要： A sensing device includes: a semiconductor layer of a field effect semiconductor having upper and lower surfaces; a conductive layer formed on the lower surface of the semiconductor layer; and a sensor layer of an insulator formed on the upper surface of the semiconductor layer. The insulator is made from lanthanide-titanium oxide.

摘要翻译： 感测装置包括：具有上表面和下表面的场效应半导体的半导体层; 形成在所述半导体层的下表面上的导电层; 以及形成在半导体层的上表面上的绝缘体的传感器层。 绝缘体由镧系元素 - 氧化钛制成。

公开(公告)号：US20110298015A1

公开(公告)日：2011-12-08

申请号：US12793647

申请日：2010-06-03

申请人： Tung-Ming Pan ,  Min-Hsien Wu ,  Ming-De Huang ,  Chao-Sung Lai

发明人： Tung-Ming Pan ,  Min-Hsien Wu ,  Ming-De Huang ,  Chao-Sung Lai

IPC分类号： H01L29/66

CPC分类号： A61B5/14539 ,  C12Q1/001 ,  G01N27/4145

摘要： A sensing device includes: a semiconductor layer of a field effect semiconductor having upper and lower surfaces; a conductive layer formed on the lower surface of the semiconductor layer; and a sensor layer of an insulator formed on the upper surface of the semiconductor layer. The insulator is made from lanthanide-titanium oxide.

摘要翻译： 感测装置包括：具有上表面和下表面的场效应半导体的半导体层; 形成在所述半导体层的下表面上的导电层; 以及形成在半导体层的上表面上的绝缘体的传感器层。 绝缘体由镧系元素 - 氧化钛制成。

公开(公告)号：US08951403B2

公开(公告)日：2015-02-10

申请号：US13478161

申请日：2012-05-23

申请人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

发明人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/22

CPC分类号： C12Q1/001 ,  G01N27/227

摘要： A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.

摘要翻译： 由使用PNIPAAm包裹酶作为生物传感器的一部分的由氧化铕形成的传感器膜的检测方法包括将1.0g NIPAAm粉末加入到20ml水中，在60℃下加热，形成NIPAAm溶液，并冷却 NIPAAm解决方案 向NIPAAm溶液中加入200μl98.7wt％的APS和50μl99wt％的TEMED，均匀混合，并使混合物反应30小时以制备透明的凝胶PNIPAAm; 向100μl1×PBS缓冲溶液中加入5mg酶，均匀混合，向缓冲液中加入100μlPNIPAAm，均匀混合缓冲溶液; 将生物传感器放置在加热器上，在37℃的恒温下加热，生物传感器是具有由EuTixOy形成的传感器膜的EIS传感器; 并进行测量。

公开(公告)号：US20130032492A1

公开(公告)日：2013-02-07

申请号：US13478161

申请日：2012-05-23

申请人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

发明人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

IPC分类号： G01N27/414

CPC分类号： C12Q1/001 ,  G01N27/227

摘要： A detection method for a sensor membrane formed of EuTixOy as part of a biosensor by using PNIPAAm for wrapping enzymes is provided with adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1× PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. wherein the biosensor is an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.

摘要翻译： 提供了通过使用PNIPAAm包裹酶作为生物传感器的一部分的EuTixOy形成的传感器膜的检测方法，在20ml水中加入1.0g NIPAAm粉末，在60℃下加热，形成NIPAAm溶液，并冷却 NIPAAm解决方案 向NIPAAm溶液中加入200μl98.7wt％的APS和50μl99wt％的TEMED，均匀混合，并使混合物反应30小时以制备透明的凝胶PNIPAAm; 向100μl1×PBS缓冲溶液中加入5mg酶，均匀混合，向缓冲液中加入100μlPNIPAAm，均匀混合缓冲溶液; 将生物传感器放置在加热器上，在37℃的恒温下加热，其中生物传感器是具有由EuTixOy形成的传感器膜的EIS传感器; 并进行测量。

公开(公告)号：US11231425B2

公开(公告)日：2022-01-25

申请号：US16567624

申请日：2019-09-11

申请人： Tzong-Shi Chiueh ,  Min-Hsien Wu ,  Hsin-Yao Wang

发明人： Tzong-Shi Chiueh ,  Min-Hsien Wu ,  Hsin-Yao Wang

IPC分类号： G01N33/68 ,  G01N33/554 ,  G01N33/569

摘要： An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of respective platelet samples.

公开(公告)号：US20120231976A1

公开(公告)日：2012-09-13

申请号：US13241123

申请日：2011-09-22

申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

IPC分类号： C40B60/14

CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

公开(公告)号：US20110217751A1

公开(公告)日：2011-09-08

申请号：US13064006

申请日：2011-03-02

申请人： Gwo-Bin Lee ,  Song-Bin Huang ,  Min-Hsien Wu

发明人： Gwo-Bin Lee ,  Song-Bin Huang ,  Min-Hsien Wu

IPC分类号： C12N11/10 ,  C08B37/04 ,  H01F1/00 ,  B82Y30/00

CPC分类号： C12N11/10 ,  B82Y30/00 ,  H01F1/00

摘要： This present invention relates to a method for forming alginate microbeads. The method includes using a needle connected to a vibrator to continuously spot tiny alginate microdroplets in an oil layer. Subsequently, the temporarily formed alginate microdroplets sink into a CaCl2 solution to become gelled microbeads. As a whole, the method has opened up a route to perform alginate-microbead formation in a simple, continuous, controllable, uniform, cell-friendly, and less-contaminated manner.

摘要翻译： 本发明涉及一种形成藻酸盐微珠的方法。 该方法包括使用连接到振动器的针连续地在油层中发现微小的藻酸盐微滴。 随后，临时形成的藻酸盐微滴沉降成CaCl 2溶液成为胶凝微珠。 总体而言，该方法开辟了以简单，连续，可控，均匀，细胞友好且污染少的方式进行藻酸盐 - 微珠形成的途径。

公开(公告)号：US10578607B2

公开(公告)日：2020-03-03

申请号：US15817431

申请日：2017-11-20

申请人： Min-Hsien Wu ,  Wen-Pin Chou ,  Tzu-Keng Chiu ,  Po-Yu Chu

发明人： Min-Hsien Wu ,  Wen-Pin Chou ,  Tzu-Keng Chiu ,  Po-Yu Chu

IPC分类号： G01N33/49 ,  C12N5/09 ,  G01N27/327 ,  G01N33/574 ,  G01N27/447 ,  C12N13/00

摘要： A method of screening, isolating and purifying rare cells includes obtaining cell groups with rare cells from a sample, removing cells not to be screened from the cell groups, and drawing the remaining cells as a cell suspension sample. An introduction device is activated to load the cell suspension sample onto an optically-induced dielectrophoresis chip including a cell isolation zone having a division arrangement wherein at least one optical pattern is configured on the cell isolation zone for screening, isolating and purifying the rare cells. The at least one optical pattern is configured to migrate, rotate or both. The at least one optical pattern is activated to attract the rare cells into a collection tank on the chip, and a liquid drawing device is activated to draw the rare cells out of the collection tank, thereby obtaining purified rare cells from the sample.

公开(公告)号：US09068281B2

公开(公告)日：2015-06-30

申请号：US13241123

申请日：2011-09-22

申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

IPC分类号： C40B60/12 ,  C12M3/06 ,  C12M1/34

CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

公开(公告)号：US08551771B2

公开(公告)日：2013-10-08

申请号：US12653335

申请日：2009-12-10

申请人： Min-Hsien Wu ,  Wan-Chen Tsai

发明人： Min-Hsien Wu ,  Wan-Chen Tsai

IPC分类号： C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/12 ,  C12M1/22

CPC分类号： C12M25/14 ,  C12M23/12

摘要： A gel molding apparatus is adapted to be used in combination with a vessel that has a plurality of solution chambers, and includes a lid plate and a plurality of columns that project from a top surface of the lid plate. Each of the columns has a bottom side connected to the top surface of the lid plate, a top side opposite to the bottom side, and a well recessed from the top side for receiving a gel suspension and having a depth from the top side. A method for gel molding is conducted via the gel molding apparatus. A culture apparatus includes the vessel and the gel molding apparatus. A method for culture is performed through the culture apparatus.

摘要翻译： 凝胶成型装置适于与具有多个溶液室的容器组合使用，并且包括盖板和从盖板的顶表面突出的多个柱。 每个柱具有连接到盖板的顶表面的底侧，与底侧相对的顶侧，以及从顶侧凹入的用于接收凝胶悬浮体并且具有从顶侧的深度的凹部。 通过凝胶成型装置进行凝胶成型的方法。 培养装置包括容器和凝胶成型装置。 通过培养装置进行培养的方法。