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公开(公告)号：US11745159B2
公开(公告)日：2023-09-05
申请号：US17122988
申请日：2020-12-15
Applicant: Twist Bioscience Corporation
Inventor： Eugene P. Marsh , Pierre F. Indermuhle , Bill James Peck
IPC: B01J19/00
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00509 , B01J2219/00533 , B01J2219/00596 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00716 , B01J2219/00722
Abstract: Defined sequence RNA synthesis by 3′→5′ direction is now well established and currently in use for synthesis and development of vast variety of therapeutic grade RNA and Si RNA etc. A number of such synthetic RNA requires a modification or labeling of 3′-end of an oligonucleotide. The synthesis of 3′-end modified RNA requiring lipophilic, long chain ligands or chromophores, using 3′→5′ synthesis methodology is challenging, requires corresponding solid support and generally results in low coupling efficiency and lower purity of the final oligonucleotide in general because of large amount of truncated sequences containing desired hydrophobic modification. We have approached this problem by developing reverse RNA monomer phosphoramidites for RNA synthesis in 5′→3′-direction. They lead to very clean oligonucleotide synthesis allowing for introduction of various modifications at the 3′-end.
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公开(公告)号：US11697668B2
公开(公告)日：2023-07-11
申请号：US16854719
申请日：2020-04-21
Applicant: Twist Bioscience Corporation
Inventor： Pierre F. Indermuhle , Eugene P. Marsh , Andres Fernandez , William Banyai , Bill J. Peck
IPC: C07H21/04 , B01J19/00 , C07H1/00 , C12P19/34 , C23C16/455
CPC classification number: C07H21/04 , B01J19/0046 , C07H1/00 , C12P19/34 , C23C16/455 , B01J2219/0059 , B01J2219/00527 , B01J2219/00596
Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
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公开(公告)号：US10894242B2
公开(公告)日：2021-01-19
申请号：US16165952
申请日：2018-10-19
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Eugene P. Marsh , Pierre F. Indermuhle , Bill James Peck
IPC: B01J19/00
Abstract: Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.
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公开(公告)号：US10384189B2
公开(公告)日：2019-08-20
申请号：US15860445
申请日：2018-01-02
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Bill James Peck , Pierre Indermuhle , Eugene P. Marsh , Andres Fernandez , David Stern
IPC: B01J19/00
Abstract: Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.
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公开(公告)号：US09981239B2
公开(公告)日：2018-05-29
申请号：US15135434
申请日：2016-04-21
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： William Banyai , Bill James Peck , Andres Fernandez , Siyuan Chen , Pierre Indermuhle , Eugene P. Marsh
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/00497 , B01J2219/00511 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00635 , B01J2219/00637 , B01J2219/00662 , B01J2219/00722 , B01L3/50857 , C12N15/1093 , C12Q2563/159 , C12Q2565/513 , C12Q2565/629
Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
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Patent Agency Ranking
公开(公告)号：US11691118B2
公开(公告)日：2023-07-04
申请号：US16921712
申请日：2020-07-06
Applicant: Twist Bioscience Corporation
Inventor： William Banyai , Bill James Peck , Andres Fernandez , Siyuan Chen , Pierre Indermuhle , Eugene P. Marsh
CPC classification number: B01J19/0046 , C12N15/1093 , B01J2219/00317 , B01J2219/00497 , B01J2219/00511 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00635 , B01J2219/00637 , B01J2219/00662 , B01J2219/00722 , B01L3/50857 , C12N15/1093 , C12Q2563/159 , C12Q2565/513 , C12Q2565/629
Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
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公开(公告)号：US20220064206A1
公开(公告)日：2022-03-03
申请号：US17458200
申请日：2021-08-26
Applicant: Twist Bioscience Corporation
Inventor： Andres FERNANDEZ , Eugene P. Marsh , Maryam HABIBIAN
Abstract: Provided herein are compositions, devices, systems and methods for electrochemical synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.
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公开(公告)号：US20210129108A1
公开(公告)日：2021-05-06
申请号：US17122988
申请日：2020-12-15
Applicant: Twist Bioscience Corporation
Inventor： Eugene P. Marsh , Pierre F. Indermuhle , Bill James Peck
IPC: B01J19/00
Abstract: Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.
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公开(公告)号：US10669304B2
公开(公告)日：2020-06-02
申请号：US15015059
申请日：2016-02-03
Applicant: TWIST BIOSCIENCE CORPORATION
Inventor： Pierre F. Indermuhle , Eugene P. Marsh , Andres Fernandez , William Banyai , Bill J. Peck
IPC: C07H21/04 , B01J19/00 , C07H1/00 , C12P19/34 , C23C16/455
Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
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公开(公告)号：US20200299322A1
公开(公告)日：2020-09-24
申请号：US16854719
申请日：2020-04-21
Applicant: Twist Bioscience Corporation
Inventor： Pierre F. Indermuhle , Eugene P. Marsh , Andres Fernandez , William Banyai , Bill J. Peck
IPC: C07H21/04 , B01J19/00 , C07H1/00 , C12P19/34 , C23C16/455
Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
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