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公开(公告)号：US11691118B2
公开(公告)日：2023-07-04
申请号：US16921712
申请日：2020-07-06
发明人： William Banyai , Bill James Peck , Andres Fernandez , Siyuan Chen , Pierre Indermuhle , Eugene P. Marsh
CPC分类号： B01J19/0046 , C12N15/1093 , B01J2219/00317 , B01J2219/00497 , B01J2219/00511 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00635 , B01J2219/00637 , B01J2219/00662 , B01J2219/00722 , B01L3/50857 , C12N15/1093 , C12Q2563/159 , C12Q2565/513 , C12Q2565/629
摘要： Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
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公开(公告)号：US20210129108A1
公开(公告)日：2021-05-06
申请号：US17122988
申请日：2020-12-15
IPC分类号： B01J19/00
摘要： Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.
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公开(公告)号：US10583415B2
公开(公告)日：2020-03-10
申请号：US16409608
申请日：2019-05-10
IPC分类号： B01J19/00 , C12N15/10 , C40B50/18 , C12N15/66 , C12N15/74 , C40B50/00 , C40B40/06 , C40B50/14 , C12N15/09
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US20150038373A1
公开(公告)日：2015-02-05
申请号：US14452429
申请日：2014-08-05
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
摘要翻译： 本文提供了从头合成的大型核酸文库，其错误率低。 此外，本文描述了用于制造高质量构建块（例如寡核苷酸）的装置。 可以使用微流控组件并行合成更长的核酸。 此外，本文的方法允许快速构建长，高质量基因的大型文库。 用于制造长质量和高质量核酸的大型文库的装置在本文中进一步描述。
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公开(公告)号：US12056264B2
公开(公告)日：2024-08-06
申请号：US17938228
申请日：2022-10-05
发明人： Bill James Peck
CPC分类号： G06F21/78 , C12N15/102 , C12N15/1027 , C12N15/1089 , G06F2212/1052 , G06F2212/402
摘要： Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.
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公开(公告)号：US11745159B2
公开(公告)日：2023-09-05
申请号：US17122988
申请日：2020-12-15
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00509 , B01J2219/00533 , B01J2219/00596 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00716 , B01J2219/00722
摘要： Defined sequence RNA synthesis by 3′→5′ direction is now well established and currently in use for synthesis and development of vast variety of therapeutic grade RNA and Si RNA etc. A number of such synthetic RNA requires a modification or labeling of 3′-end of an oligonucleotide. The synthesis of 3′-end modified RNA requiring lipophilic, long chain ligands or chromophores, using 3′→5′ synthesis methodology is challenging, requires corresponding solid support and generally results in low coupling efficiency and lower purity of the final oligonucleotide in general because of large amount of truncated sequences containing desired hydrophobic modification. We have approached this problem by developing reverse RNA monomer phosphoramidites for RNA synthesis in 5′→3′-direction. They lead to very clean oligonucleotide synthesis allowing for introduction of various modifications at the 3′-end.
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公开(公告)号：US10272410B2
公开(公告)日：2019-04-30
申请号：US14885965
申请日：2015-10-16
IPC分类号： B01J19/00 , C12N15/10 , C40B50/18 , C12N15/66 , C12N15/74 , C40B50/00 , C40B40/06 , C40B50/14 , C12N15/09
摘要： Provided herein are devices for the manufacturing of high-quality oligonucleic acids. Longer nucleic acids, e.g., genes, can be synthesized in parallel using microfluidic assemblies described herein. Devices described herein include silicon plates having a plurality of channels in fluid communication with a plurality of microchannels. The number of microchannels and dimensions of the microchannels provide for rapid exchange of chemical exposure during de novo synthesis of oligonucleic acids.
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公开(公告)号：US20180264428A1
公开(公告)日：2018-09-20
申请号：US15991992
申请日：2018-05-29
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US09833761B2
公开(公告)日：2017-12-05
申请号：US14885962
申请日：2015-10-16
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US09403141B2
公开(公告)日：2016-08-02
申请号：US14885963
申请日：2015-10-16
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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