公开(公告)号：US20070148746A1

公开(公告)日：2007-06-28

申请号：US11565696

申请日：2006-12-01

申请人： ULRICH SCHOERKEN ,  Carolin Meyer ,  Peter Horlacher ,  Sabine Both

发明人： ULRICH SCHOERKEN ,  Carolin Meyer ,  Peter Horlacher ,  Sabine Both

IPC分类号： C12P7/64 ,  C07C57/02

CPC分类号： C12P7/6472 ,  C12P7/6454

摘要： The invention relates to a process for the enzymatic synthesis of esters of polyunsaturated, short-chain or sensitive fatty acids with a high triglyceride content, which is made up of an enzymatic synthesis of the mixture of triglycerides and partial glycerides starting from free fatty acid, methyl or ethyl esters and glycerol, a subsequent selective enzymatic back-hydrolysis of the partial glycerides to fatty acid and glycerol and distillation-based separation of the triglycerides, and to the triglyceride-rich ester mixtures obtainable by this process. The process is preferably carried out as a one-pot process in the same reactor and in the presence of the same enzyme.

摘要翻译： 本发明涉及一种用于酶促合成具有高甘油三酯含量的多不饱和，短链或敏感脂肪酸酯的方法，其由甘油三酯和偏甘油酯从游离脂肪酸开始的混合物的酶合成， 甲基或乙酯和甘油，随后将部分甘油酯选择性酶促回水解成脂肪酸和甘油，并且基于蒸馏的甘油三酸酯分离，以及通过该方法可获得的富含甘油三酯的酯混合物。 该方法优选在同一反应器中和在相同酶存在下作为一锅法进行。

公开(公告)号：US07981641B2

公开(公告)日：2011-07-19

申请号：US12061005

申请日：2008-04-02

申请人： Ulrich Schoerken ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

发明人： Ulrich Schoerken ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

IPC分类号： C12P7/64 ,  C08L91/00

CPC分类号： C12P7/6472

摘要： Processes for the enzyme-catalyzed production of triglycerides using polyunsaturated fatty acids, in which (a) the reaction of polyunsaturated fatty acids and/or C1-4 alkyl esters thereof with glycerol in vacuo in the presence of an immobilized enzyme to form their triglycerides is accelerated by addition of an additive from the group of weakly basic salts, complexing agents and ion exchangers and/or addition of a weakly basic salt and/or addition of an entraining agent in the form of a solvent or a gas and/or addition of glycerol-binding adsorbers and/or heat treatment of the partial glyceride intermediate product, (b) the immobilized enzymes are removed from the triglyceride by separation or filtration and (c) the remaining fatty acids and/or C1-4 alkyl esters thereof are removed from the triglyceride by distillation, refining or extraction.

摘要翻译： 使用多不饱和脂肪酸进行酶催化生产甘油三酸酯的方法，其中（a）在固定化酶存在下，多不饱和脂肪酸和/或其中C 1-4烷基酯与甘油在真空中反应形成其甘油三酸酯的方法是 通过加入来自弱碱性盐，络合剂和离子交换剂的添加剂和/或加入弱碱性盐和/或以溶剂或气体的形式加入夹带剂和/或添加 甘油结合吸附剂和/或热处理部分甘油酯中间产物，（b）通过分离或过滤从甘油三酯中除去固定化的酶，和（c）除去剩余的脂肪酸和/或其中的C 1-4烷基酯 从甘油三酯通过蒸馏，精制或提取。

公开(公告)号：US20080182304A1

公开(公告)日：2008-07-31

申请号：US12061005

申请日：2008-04-02

申请人： ULRICH SCHOERKEN ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

发明人： ULRICH SCHOERKEN ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

IPC分类号： C12P7/64

CPC分类号： C12P7/6472

摘要： Processes for the enzyme-catalyzed production of triglycerides using polyunsaturated fatty acids, in which (a) the reaction of polyunsaturated fatty acids and/or C1-4 alkyl esters thereof with glycerol in vacuo in the presence of an immobilized enzyme to form heir triglycerides is accelerated by addition of an additive from the group of weakly basic salts, complexing agents and ion exchangers and/or addition of a weakly basic salt and/or addition of an entraining agent in the form of a solvent or a gas and/or addition of glycerol-binding adsorbers and/or heat treatment of the partial glyceride intermediate product, (b) the immobilized enzymes are removed from the triglyceride by separation or filtration and (c) the remaining fatty acids and/or C1-4 alkyl esters thereof are removed from the triglyceride by distillation, refining or extraction.

摘要翻译： 使用多不饱和脂肪酸酶催化生产甘油三酸酯的方法，其中（a）多不饱和脂肪酸和/或其中C 1-4烷基酯与甘油在真空中的存在下反应， 通过从弱碱性盐，络合剂和离子交换剂组中加入添加剂和/或加入弱碱性盐和/或加入溶剂形式的夹带剂来加速形成继承甘油三酯的固定化酶 或甘油结合吸附剂的气体和/或加成物和/或热处理部分甘油酯中间产物，（b）通过分离或过滤从甘油三酯中除去固定化的酶，和（c）剩余的脂肪酸和/或 通过蒸馏，精制或萃取，从甘油三酯中除去C 1-4烷基酯。

公开(公告)号：US20050233426A1

公开(公告)日：2005-10-20

申请号：US11093410

申请日：2005-03-30

申请人： Ulrich Schoerken ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

发明人： Ulrich Schoerken ,  Sabine Both ,  Carolin Meyer ,  Peter Horlacher

IPC分类号： C11C3/06 ,  C11C3/10 ,  C12P7/64

CPC分类号： C12P7/6472

摘要： Processes for the enzyme-catalyzed production of triglycerides using polyunsaturated fatty acids, in which (a) the reaction of polyunsaturated fatty acids and/or C1-4 alkyl esters thereof with glycerol in vacuo in the presence of an immobilized enzyme to form their triglycerides is accelerated by addition of an additive from the group of weakly basic salts, complexing agents and ion exchangers and/or addition of a weakly basic salt and/or addition of an entraining agent in the form of a solvent or a gas and/or addition of glycerol-binding adsorbers and/or heat treatment of the partial glyceride intermediate product, (b) the immobilized enzymes are removed from the triglyceride by separation or filtration and (c) the remaining fatty acids and/or C1-4 alkyl esters thereof are removed from the triglyceride by distillation, refining or extraction.

摘要翻译： 使用多不饱和脂肪酸酶催化生产甘油三酸酯的方法，其中（a）多不饱和脂肪酸和/或其中C 1-4烷基酯与甘油在真空中的存在下反应， 通过从弱碱性盐，络合剂和离子交换剂组中加入添加剂和/或加入弱碱性盐和/或以溶剂形式加入夹带剂来加速形成它们的甘油三酯的固定化酶 或甘油结合吸附剂的气体和/或加成物和/或热处理部分甘油酯中间产物，（b）通过分离或过滤从甘油三酯中除去固定化的酶，和（c）剩余的脂肪酸和/或 通过蒸馏，精制或萃取，从甘油三酯中除去C 1-4烷基酯。

公开(公告)号：US20050233427A1

公开(公告)日：2005-10-20

申请号：US11093534

申请日：2005-03-30

申请人： Ulrich Schoerken ,  Carolin Meyer ,  Sabine Both ,  Peter Horlacher

发明人： Ulrich Schoerken ,  Carolin Meyer ,  Sabine Both ,  Peter Horlacher

IPC分类号： C11C1/04 ,  C11C3/06 ,  C11C3/10 ,  C12P7/64

CPC分类号： C12P7/6472 ,  C11C3/06

摘要： Processes for the enzyme-catalyzed synthesis of triglycerides containing polyunsaturated fatty acids in which the synthesis is preceded by a pre-hydrolysis in which (a) C1-4 alkyl esters of polyunsaturated fatty acids are completely or partly hydrolyzed in vacuo with continuous addition of water or are partly hydrolyzed in several stages without vacuum, (b) after the hydrolysis, the water is removed by separation or distillation and (c) the synthesis of the polyunsaturated fatty acids with glycerol in vacuo in the presence of an enzyme to form their triglycerides is carried out under low-water conditions, (d) the enzymes are removed from the triglyceride by separation or filtration and (e) the remaining fatty acids and/or possible residues of C1-4 alkyl esters are removed from the triglyceride by distillation or refining; the pre-hydrolysis and the subsequent synthesis preferably being carried out as a one-pot process in the same reactor and in the presence of the same enzyme.

摘要翻译： 用于酶催化合成含有多不饱和脂肪酸的甘油三酯的方法，其中合成之前是预水解，其中（a）多不饱和脂肪酸的C 1-4烷基酯是完全或部分 在不加入水的情况下在真空中水解或在几个阶段内没有真空部分水解，（b）水解后，通过分离或蒸馏除去水，和（c）在真空中合成甘油的多不饱和脂肪酸 在低水条件下进行酶的形成甘油三酯的存在，（d）通过分离或过滤从甘油三酯中除去酶，和（e）C 1的剩余脂肪酸和/或可能的残基 通过蒸馏或精制从甘油三酯中除去-4.0烷基酯; 预水解和随后的合成优选在相同的反应器中和在相同的酶的存在下作为一锅法进行。

公开(公告)号：US07604966B2

公开(公告)日：2009-10-20

申请号：US11554799

申请日：2006-10-31

申请人： Ulrich Schoerken ,  Doris Bell ,  Peter Horlacher ,  Diana Stuhlmann ,  Carolin Meyer

发明人： Ulrich Schoerken ,  Doris Bell ,  Peter Horlacher ,  Diana Stuhlmann ,  Carolin Meyer

IPC分类号： C12P7/64

CPC分类号： C11C3/10 ,  A21D2/165 ,  A23D9/00 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12

摘要： The invention relates to structured lipid mixtures corresponding to formula (I): characterized in that R1CO, R2CO and R3CO independently of one another represent (i) linear saturated acyl groups containing 6 to 12 carbon atoms or (ii) the acyl group of conjugated linoleic acid (CLA) and/or an omega-3 or omega-6 fatty acid (OF), with the proviso that the quantity of CLA acyl groups amounts to 3 to 50 mol- % and the quantity of OF acyl groups to 5 to 25 mol- %, based on the quantity of acyl groups. The mixtures are preferably made by subjecting a mixture of component (a) a medium-chain triglyceride; and component (b) a CLA, an (OF), a TG-CLA (TG-OF) or a mixture thereof to enzymatic transesterification in the presence of a vegetable, marine or microbial oil.

摘要翻译： 本发明涉及对应于式（I）的结构化脂质混合物：其特征在于R1CO，R2CO和R3CO彼此独立地表示（i）含有6至12个碳原子的直链饱和酰基或（ii）共轭亚油酸的酰基 酸（CLA）和/或ω-3或ω-6脂肪酸（OF），条件是CLA酰基的量为3〜50摩尔％，酰基的量为5〜25 mol％，基于酰基的数量。 混合物优选通过将组分（a），中链甘油三酯的混合物， 和（b）CLA，（OF），TG-CLA（TG-OF）或其混合物在植物，海洋或微生物油的存在下进行酶促酯交换反应。

公开(公告)号：US20070111292A1

公开(公告)日：2007-05-17

申请号：US11554799

申请日：2006-10-31

申请人： Ulrich Schoerken ,  Doris Bell ,  Peter Horlacher ,  Diana Stuhlmann ,  Carolin Meyer

发明人： Ulrich Schoerken ,  Doris Bell ,  Peter Horlacher ,  Diana Stuhlmann ,  Carolin Meyer

IPC分类号： A61K31/22 ,  C12P7/64

CPC分类号： C11C3/10 ,  A21D2/165 ,  A23D9/00 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12

摘要： The invention relates to structured lipid mixtures corresponding to formula (I): characterized in that R1CO, R2CO and R3CO independently of one another represent (i) linear saturated acyl groups containing 6 to 12 carbon atoms or (ii) the acyl group of conjugated linoleic acid (CLA) and/or an omega-3 or omega-6 fatty acid (OF), with the proviso that the quantity of CLA acyl groups amounts to 3 to 50 mol-% and the quantity of OF acyl groups to 5 to 25 mol-%, based on the quantity of acyl groups. The mixtures are preferably made by subjecting a mixture of component (a) a medium-chain triglyceride; and component (b) a CLA, an (OF), a TG-CLA (TG-OF) or a mixture thereof to enzymatic transesterification in the presence of a vegetable, marine or microbial oil.

摘要翻译： 本发明涉及对应于式（I）的结构化脂质混合物：其特征在于R 1 CO，R 2 CO和R 3 CO独立地 彼此表示（i）含有6至12个碳原子的直链饱和酰基或（ii）共轭亚油酸（CLA）和/或ω-3或ω-6脂肪酸（OF）的酰基，其中 条件是基于酰基的量，CLA酰基的量为3至50摩尔％，酰基的量为5至25摩尔％。 混合物优选通过将组分（a），中链甘油三酯的混合物， 和（b）CLA，（OF），TG-CLA（TG-OF）或其混合物在植物，海洋或微生物油的存在下进行酶促酯交换反应。

公开(公告)号：US20080248187A1

公开(公告)日：2008-10-09

申请号：US12061276

申请日：2008-04-02

申请人： Ulrich Schoerken ,  Peter Kempers ,  Andreas Sander ,  Peter Horlacher

发明人： Ulrich Schoerken ,  Peter Kempers ,  Andreas Sander ,  Peter Horlacher

IPC分类号： A23L1/29 ,  C12P7/64 ,  C07C55/00

CPC分类号： C12P7/6454 ,  A23D9/013 ,  A23D9/02 ,  A23K20/158 ,  A23K50/80 ,  A23L33/12 ,  A23V2002/00 ,  C11C3/003 ,  C11C3/10 ,  A23V2200/30 ,  A23V2250/182 ,  A23V2250/1942 ,  A23V2250/192

摘要： A mixture containing fatty acid glycerides which has a high percentage content of PUFA acyl groups and a low percentage content of saturated fatty acid acyl groups is described. A process which enables the PUFA acyl groups in a mixture containing fatty acid glycerides (for example a fish oil) to be enriched and, at the same time, the content of saturated fatty acid acyl groups to be maintained at a low content is described. The process is a hydrolytic process or an alcoholysis in which the fatty acid acyl groups to be enriched are hydrolytically or alcoholytically released from the fatty acid glycerides slowly, if at all, the process being carried out in the presence of a lipase.

摘要翻译： 描述了包含脂肪酸甘油酯的混合物，其具有高百分比含量的PUFA酰基和低百分含量的饱和脂肪酸酰基。 描述了使包含脂肪酸甘油酯（例如鱼油）的混合物中的PUFA酰基富集并且同时使饱和脂肪酸酰基含量维持在低含量的方法。 该方法是水解方法或醇解，其中待富集的脂肪酸酰基缓慢地从脂肪酸甘油酯水解或醇解地释放，如果完全是在脂肪酶存在下进行的。

公开(公告)号：US20130331588A1

公开(公告)日：2013-12-12

申请号：US14000443

申请日：2012-03-03

申请人： Dieter Hietsch ,  Peter Horlacher

发明人： Dieter Hietsch ,  Peter Horlacher

IPC分类号： C07C67/54 ,  C07C69/587

CPC分类号： C07C67/54 ,  C07C67/60 ,  C07C69/587 ,  C11C1/10 ,  C11C3/003

摘要： The present invention relates to a method for working up a mixture which contains esters of EPA and/or DHA with monohydric alcohols having 1 to 6 carbon atoms, esters of other fatty acids with monohydric alcohols having 1 to 6 carbon atoms, and free cholesterol, wherein the method comprises adding a transesterification catalyst to the mixture, converting at least some of the free cholesterol into esterified cholesterol and thereafter distilling the mixture, wherein the distillation is carried out in such a manner that a product is obtained which contains EPA and/or DHA, both in the form of esters thereof with monohydric alcohols having 1 to 6 carbon atoms, in a higher relative amount, based on all fatty acids in free or bound form present in the product, than said mixture. In addition, the present invention relates to a product which is obtainable by the method according to the invention, wherein the product contains 10 to 99.99% by weight of esters of EPA and/or of DHA with monohydric alcohols having 1 to 6 carbon atoms and 0.0001 to a maximum of 0.3% by weight of cholesterol in free or bound form.

摘要翻译： 本发明涉及一种用于处理含有EPA和/或DHA的酯与具有1至6个碳原子的一元醇的混合物的混合物的方法，其它脂肪酸与具有1至6个碳原子的一元醇的酯和游离胆固醇， 其中所述方法包括向所述混合物中加入酯交换催化剂，将至少一些游离胆固醇转化为酯化胆固醇，然后蒸馏混合物，其中蒸馏是以获得含有EPA和/或 DHA，以其与具有1至6个碳原子的一元醇的酯的形式，以相对于所述混合物中存在的游离或结合形式的所有脂肪酸的相对量较高。 另外本发明涉及通过本发明的方法获得的产品，其中该产品含有10至99.99重量％的EPA和/或DHA的酯与具有1至6个碳原子的一元醇的酯和 0.0001至最大0.3重量％的游离或结合形式的胆固醇。

公开(公告)号：US08399042B2

公开(公告)日：2013-03-19

申请号：US12746626

申请日：2008-11-28

申请人： Rainer Eskuchen ,  Jörg Schwarzer ,  Peter Horlacher ,  Jürgen Gierke ,  Robert Salacz

发明人： Rainer Eskuchen ,  Jörg Schwarzer ,  Peter Horlacher ,  Jürgen Gierke ,  Robert Salacz

IPC分类号： A23D7/00

CPC分类号： A23L2/00 ,  A23L29/10 ,  A23L33/10 ,  A23L33/11 ,  A23L33/12 ,  A23P10/47 ,  A23V2002/00 ,  A23V2200/222 ,  A23V2250/2136

摘要： The invention relates to aqueous dispersions containing (a) 5 to 50% by weight of sterols and/or stanols; and (b) 0.5 to 20% by weight of citric acid esters of mono- and/or diglycerides, based on the total weight of the dispersion, wherein the citric acid esters of mono- and/or diglycerides are partially neutralised; and to a process for the production of an aqueous sterol/stanol-containing dispersion, which process comprises (a) dissolving citric acid esters of mono- and/or diglycerides that are partly neutralised, in water; (b) dispersing sterols and/or stanols in the emulsifier solution to form a pre-dispersion; and (c) grinding the pre-dispersion in a ball-mill until the dispersion has a particle mass diameter, d90, of less than 5 μm. Optionally, this dispersion is spray-dried.

摘要翻译： 本发明涉及含有（a）5至50重量％的甾醇和/或甾醇的水分散体; 和（b）基于分散体总重量的0.5至20重量％的甘油单酯和/或甘油二酯的柠檬酸酯，其中甘油单酯和/或甘油二酯的柠檬酸酯部分中和; 以及生产含水甾醇/含Stanol的分散体的方法，该方法包括（a）将部分中和的甘油单酯和/或甘油二酯的柠檬酸酯溶解在水中; （b）将甾醇和/或甾醇分散在乳化剂溶液中以形成预分散体; 和（c）在球磨机中研磨预分散体，直到分散体具有小于5μm的颗粒质量直径d 90。 任选地，将该分散体喷雾干燥。