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    METHOD FOR CULTURING SKELETAL MUSCLE FOR TISSUE ENGINEERING
    2.
    发明申请
    METHOD FOR CULTURING SKELETAL MUSCLE FOR TISSUE ENGINEERING 审中-公开
    用于组织工程的骨骼肌肉培养方法

    公开(公告)号：US20160068812A1

    公开(公告)日：2016-03-10

    申请号：US14886577

    申请日：2015-10-19

    Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

    Inventor： James J. Hickman ,  Mainak Das ,  John W. Rumsey

    IPC: C12N5/077

    CPC classification number: C12N5/0652 ,  C12N5/0619 ,  C12N5/0658 ,  C12N2500/05 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/40 ,  C12N2502/1335 ,  C12N2533/30

    Abstract: The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating. A cell nutrient medium composition includes Neurobasal, an antibiotic-antimycotic composition, cholesterol, human TNF-alpha, PDGF BB, vasoactive intestinal peptides, insulin-like growth factor 1, NAP, r-Apolipoprotein E2, purified mouse Laminin, beta amyloid, human tenascin-C protein, rr-Sonic hedgehog Shh N-terminal, and rr-Agrin C terminal.

    Abstract translation: 本发明提供了一种营养培养基组合物和相关方法，用于延长肌肉细胞培养物的使用寿命。 公开了一种培养哺乳动物肌肉细胞的方法，包括制备一种或多种涂覆有共价键合的三甲氧基 - 甲硅烷基丙基 - 二亚乙基三胺（DETA）单层的载体; 通过一个或多个相关联的光学参数验证DETA单层形成; 根据培养基组成1，在无血清培养基中悬浮分离的胎鼠大鼠骨骼肌细胞; 以预定的密度将悬浮细胞电镀到制备的载体上; 使载体不受干扰以使细胞粘附到DETA单层上; 用介质1和介质2的混合物覆盖载体; 并孵化。 细胞营养培养基组合物包括Neurobasal，抗生素 - 抗真菌组合物，胆固醇，人TNF-α，PDGF BB，血管活性肠肽，胰岛素样生长因子1，NAP，r-载脂蛋白E2，纯化的小鼠层粘连蛋白，β淀粉样蛋白，人 腱生蛋白-C蛋白，rr-Sonic刺猬Shh N末端和rr-Agrin C端。

    METHOD AND SYSTEM FOR PRINTING CELLS TO A SUBSTRATE COMPRISING CELL ADHESIVE REGIONS
    7.
    发明申请
    METHOD AND SYSTEM FOR PRINTING CELLS TO A SUBSTRATE COMPRISING CELL ADHESIVE REGIONS 审中-公开

    公开(公告)号：US20200048602A1

    公开(公告)日：2020-02-13

    申请号：US16534624

    申请日：2019-08-07

    Applicant: University of Central Florida Research Foundation, Inc. ,  Hesperos, Inc.

    Inventor： James J. Hickman ,  Sandra Rothemund ,  Megan Aubin ,  Frank Alexander

    IPC: C12N5/00

    Abstract: The method of culturing cells disclosed herein includes printing cells onto a substrate that includes cell adhesive regions and cell repulsive regions. The cells are suspended in a printing medium to create a cell suspension, and a volume of the cell suspension is loaded into a printer. A cell adhesive region of the substrate is aligned beneath the printing channel of the printer, and droplets of the cell suspension are dispensed from the printing channel directly onto the cell adhesive region. Contact of the dispensed droplets with cell repulsive regions of the substrate is limited, either by targeting of the droplets to the cell adhesive regions, by repulsions generated by the cell repulsive areas, or both. The cells adhere to the cell adhesive regions to create a cell pattern, and are maintained thereafter in a physiologically suitable environment.

    Method for culturing skeletal muscle for tissue engineering
    8.
    发明授权
    Method for culturing skeletal muscle for tissue engineering 有权

    公开(公告)号：US10160953B2

    公开(公告)日：2018-12-25

    申请号：US14886577

    申请日：2015-10-19

    Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

    Inventor： James J. Hickman ,  Mainak Das ,  John W. Rumsey

    IPC: C12N5/077 ,  C12N5/0793

    Abstract: The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating. A cell nutrient medium composition includes Neurobasal, an antibiotic-antimycotic composition, cholesterol, human TNF-alpha, PDGF BB, vasoactive intestinal peptides, insulin-like growth factor 1, NAP, r-Apolipoprotein E2, purified mouse Laminin, beta amyloid, human tenascin-C protein, rr-Sonic hedgehog Shh N-terminal, and rr-Agrin C terminal.

    NOCICEPTOR-LIKE CELLS DIFFERENTIATED FROM HUMAN NEURAL PROGENITORS AND USES THEREOF
    9.
    发明申请
    NOCICEPTOR-LIKE CELLS DIFFERENTIATED FROM HUMAN NEURAL PROGENITORS AND USES THEREOF 审中-公开
    来自人类神经前体的免疫细胞样细胞及其用途

    公开(公告)号：US20170045499A1

    公开(公告)日：2017-02-16

    申请号：US15235867

    申请日：2016-08-12

    Applicant: University of Central Florida Research Foundation, Inc.

    Inventor： James J. Hickman ,  Xiufang Guo

    IPC: G01N33/50 ,  C12N5/0793

    CPC classification number: G01N33/5058 ,  C12N5/062 ,  C12N2500/90 ,  C12N2501/15 ,  C12N2503/06

    Abstract: Disclosed herein are methods of differentiating human neural progenitor cells to nociceptor-like cells. Also disclosed are methods of making an innervated skin-like construct using nociceptor-like cells differentiated from human neural progenitor cells. Also disclosed are engineered constructs for screening potentially therapeutic compounds that include a skin-like construct and nociceptor-like cells differentiated from human neural progenitor cells. Also disclosed is a method of screening potential therapies.

    Abstract translation: 本文公开了将人神经祖细胞分化为伤害感受器样细胞的方法。 还公开了使用从人神经祖细胞分化的伤害感受器样细胞制备神经支配皮肤样构建体的方法。 还公开了用于筛选潜在的治疗性化合物的工程构建体，其包括皮肤样构建体和从人神经祖细胞分化的伤害感受器样细胞。 还公开了筛选潜在疗法的方法。

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