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1.Method for diversifying the chemical composition of proteins produced in vivo by genetically disabling the editing function of their aminoacyl tRNA synthetases 失效
标题翻译: 通过遗传上禁用其氨基tRNA合成酶的编辑功能使体内产生的蛋白质的化学成分多样化的方法公开(公告)号:US07524646B2
公开(公告)日:2009-04-28
申请号:US11054192
申请日:2005-02-08
申请人: Volker Doring , Leslie A. Nangle , Tamara L. Hendrickson , Valerie De Crecy-Lagard , Paul Schimmel , Philippe Marliere
发明人: Volker Doring , Leslie A. Nangle , Tamara L. Hendrickson , Valerie De Crecy-Lagard , Paul Schimmel , Philippe Marliere
摘要: The present invention is directed to a method to diversify the chemical composition of proteins produced in vivo comprising the step of disabling, particularly by mutagenesis, the editing function of one of its aminoacyl tRNA synthetases. The present invention is also directed to nucleic acid sequences encoding such mutated aminoacyl tRNA synthetases having their editing site mutated and capable of mischarging its cognate tRNA with a noncanonical amino acid. Also described herein is an improved method for obtaining transformed cells capable of synthetizing in vivo proteins comprising at least a noncanonical amino acid and their use for the production of such proteins.
摘要翻译: 本发明涉及使体内产生的蛋白质的化学组成多样化的方法,其包括特别是通过诱变使其氨酰tRNA合成酶之一的编辑功能失活的步骤。 本发明还涉及编码这种突变的氨基酰基tRNA合成酶的核酸序列,其具有突变的编辑位点并且能够与非科学氨基酸对其同源tRNA进行错配。 本文还描述了一种用于获得能够合成体内蛋白质的转化细胞的改进方法,其包含至少一种非科学氨基酸及其用于产生这种蛋白质的用途。
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2.Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use 失效
标题翻译: 参与链霉素生物合成的多肽,编码这些多肽的核苷酸序列及其用途公开(公告)号:US5891695A
公开(公告)日:1999-04-06
申请号:US403852
申请日:1995-05-10
申请人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
发明人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
IPC分类号: C12N15/09 , C07K5/078 , C07K7/06 , C07K14/37 , C12N1/21 , C12N9/00 , C12N9/06 , C12N9/10 , C12N15/31 , C12N15/52 , C12P17/18 , C12P21/04 , C12R1/01 , C07H21/04 , C12N1/20
CPC分类号: C12N9/0012 , C07K5/06182 , C07K7/06 , C12N15/52 , C12N9/1085 , C12N9/93 , C12R1/465
摘要: The invention concerns nucleotide sequences coding for a polypeptide involved in Streptogramin biosynthesis, recombinant cells containing said sequences, and use thereof.
摘要翻译: PCT No.PCT / FR93 / 00923 Sec。 371日期:1995年5月10日 102(e)日期1995年5月10日PCT提交1993年9月25日PCT公布。 出版物WO94 / 08014 日期1994年04月14日本发明涉及编码参与链霉素生物合成的多肽的核苷酸序列,含有所述序列的重组细胞及其用途。
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3.Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use 失效
标题翻译: 参与链霉素生物合成的多肽,编码这些多肽的核苷酸序列及其用途公开(公告)号:US06670157B1
公开(公告)日:2003-12-30
申请号:US09635359
申请日:2000-08-09
申请人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
发明人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
IPC分类号: C12P1714
CPC分类号: C12R1/465 , C07K5/06182 , C07K7/06 , C12N9/0012 , C12N9/1085 , C12N9/93 , C12N15/52 , C12P17/14
摘要: The invention concerns methods for preparing pristinamycin IIB and methods for preparing modified pristinamycin IIB.
摘要翻译: 本发明涉及制备普瑞霉素IIB的方法和制备改性的原始霉素霉素IIB的方法。
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4.Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use 有权
标题翻译: 参与链霉素生物合成的多肽,编码这些多肽的核苷酸序列及其用途公开(公告)号:US07090999B2
公开(公告)日:2006-08-15
申请号:US10716803
申请日:2003-11-20
申请人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
发明人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
IPC分类号: C12P17/00
CPC分类号: C12R1/465 , C07K5/06182 , C07K7/06 , C12N9/0012 , C12N9/1085 , C12N9/93 , C12N15/52 , C12P17/14
摘要: The invention concerns methods for preparing pristinamycin IIA or IIB.
摘要翻译: 本发明涉及制备嘌呤霉素IIA或IIB的方法。
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5.Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use 有权
标题翻译: 涉及链霉素生物合成的多肽,编码这些多肽的核苷酸序列及其用途公开(公告)号:US06171846B2
公开(公告)日:2001-01-09
申请号:US09231818
申请日:1999-01-15
申请人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia LaCroix , Denis Thibaut , Monique Zagorec , Laurent DeBussche , Valerie De Crecy-Lagard
发明人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia LaCroix , Denis Thibaut , Monique Zagorec , Laurent DeBussche , Valerie De Crecy-Lagard
IPC分类号: C12N120
CPC分类号: C12N9/0012 , C07K5/06182 , C07K7/06 , C12N9/1085 , C12N9/93 , C12N15/52 , C12R1/465
摘要: The invention concerns mutant microorganisms blocked in a step of Streptogramin biosynthesis and methods for preparing cells blocked in a step of Streptogramin biosynthesis.
摘要翻译: 本发明涉及在链霉素生物合成步骤中被阻断的突变体微生物以及在Streptogramin生物合成步骤中制备细胞的方法。
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6.Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use 失效
标题翻译: 参与链霉素生物合成的多肽,编码这些多肽的核苷酸序列及其用途公开(公告)号:US6077699A
公开(公告)日:2000-06-20
申请号:US510646
申请日:1995-08-03
申请人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
发明人: Veronique Blanc , Francis Blanche , Joel Crouzet , Nathalie Jacques , Patricia Lacroix , Denis Thibaut , Monique Zagorec , Laurent Debussche , Valerie De Crecy-Lagard
CPC分类号: C12N9/0012 , C07K5/06182 , C07K7/06 , C12N15/52 , C12N9/1085 , C12N9/93 , C12R1/465
摘要: Disclosed is the nucleic acid sequences for polypeptides and enzymes involved in the biosynthesis of streptogramins from Streptomyces sp. and the corresponding amino acid sequences. Also disclosed are recombinant vectors and cells as well as methods of making the polypeptides, enzymes and streptogramins using the recombinant host cells.
摘要翻译: 公开了涉及从链霉菌属(Streptomyces sp。)生物合成链霉素的多肽和酶的核酸序列。 和相应的氨基酸序列。 还公开了重组载体和细胞以及使用重组宿主细胞制备多肽,酶和链霉素的方法。
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