公开(公告)号：US12259369B2

公开(公告)日：2025-03-25

申请号：US17057361

申请日：2019-05-20

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Thomas S. McDonald ,  Joseph D. Michienzi ,  Jeffrey Musacchio ,  Rachel Mumma ,  Keith Fadgen ,  Geoff C. Gerhardt

IPC: G01N30/06 ,  B01D15/16 ,  B01D15/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/288 ,  B01J20/32 ,  G01N1/40 ,  G01N30/00 ,  G01N30/54 ,  G01N30/60 ,  G01N30/88

Abstract: Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

公开(公告)号：US12247962B2

公开(公告)日：2025-03-11

申请号：US17057242

申请日：2019-05-20

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Thomas S. McDonald ,  Joseph D. Michienzi ,  Jeffrey Musacchio ,  Rachel Mumma ,  Keith Fadgen ,  Geoff C. Gerhardt

IPC: G01N30/60 ,  G01N30/00 ,  G01N30/06

Abstract: Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge (201) comprising a barrel (204) and a column (205), and especially including reinforcement to the column permitting high-pressure separation.

公开(公告)号：US20210396722A1

公开(公告)日：2021-12-23

申请号：US17354296

申请日：2021-06-22

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Martin Gilar ,  Joseph D. Michienzi ,  Wade P. Leveille ,  Geoff C. Gerhardt ,  Jeffrey Musacchio ,  Jason F. Hill ,  Sylvain G. Cormier

IPC: G01N30/46 ,  G01N30/30 ,  G01N30/32

Abstract: Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.

公开(公告)号：US20210319994A1

公开(公告)日：2021-10-14

申请号：US17225514

申请日：2021-04-08

Applicant: Waters Technologies Corporation

Inventor： Joseph A. Jarrell ,  Martin Gilar

IPC: H01J49/02 ,  G01N27/623 ,  H01J49/40 ,  H01J49/16 ,  H01J49/00

Abstract: An ion detector comprises a surface configured to receive one or more ions and a detector configured to detect one or more ions by detecting electromagnetic radiation scattered by one or more ions at the surface.

公开(公告)号：US20180021779A1

公开(公告)日：2018-01-25

申请号：US15702007

申请日：2017-09-12

Applicant: Waters Technologies Corporation

Inventor： Bernard Bunner ,  Martin Gilar ,  Robert A. Jencks ,  Charles T. Murphy

IPC: B01L3/00 ,  G01N30/60

Abstract: A system that incorporates teachings of the subject disclosure may include, for example, a process that includes obtaining a porous medium comprising a porous material having a first shape and an initial porosity profile. The porous medium is engaged with a cavity in a fluidic device, wherein the cavity is in fluid communication with a channel of the fluidic device. The first shape of the porous material can be adjusted to a second shape resulting in the initial porosity profile being adjusted to a target porosity profile. Such adjustment can be accomplished by the engaging of the porous medium with the cavity, by pre-adjusting a shape of the porous media before insertion into the cavity, or by some combination thereof. Other embodiments are disclosed.

公开(公告)号：US12163934B2

公开(公告)日：2024-12-10

申请号：US17354234

申请日：2021-06-22

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Martin Gilar ,  Joseph D. Michienzi ,  Wade P. Leveille ,  Geoff C. Gerhardt ,  Jeffrey Musacchio ,  Jason F. Hill ,  Sylvain G. Cormier

IPC: G01N30/46 ,  G01N30/02 ,  G01N30/30 ,  G01N30/32 ,  G01N30/72 ,  G01N30/74

Abstract: The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.

公开(公告)号：US20240240170A1

公开(公告)日：2024-07-18

申请号：US18414679

申请日：2024-01-17

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20240189740A1

公开(公告)日：2024-06-13

申请号：US18437482

申请日：2024-02-09

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Armand Gatien Ngounou Wetie

IPC: B01D15/38 ,  G01N30/34

CPC classification number: B01D15/3828 ,  G01N30/34

Abstract: The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.

公开(公告)号：US20240141429A1

公开(公告)日：2024-05-02

申请号：US18498328

申请日：2023-10-31

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Catalin Doneanu ,  Matthew A. Lauber ,  Mame Maissa Gaye

IPC: C12Q1/6876 ,  C12Q1/6806

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2600/166

Abstract: The present disclosure describes methods for analyzing mRNA poly(A) tail sequences with a high resolution to determine poly(A) tail length and heterogeneity. The method involves digesting an mRNA molecule to liberate a poly(A) tail, preparing a chromatographic sample comprising the mRNA poly(A) tails, preparing a second chromatographic sample comprising a reference sequence comprising a mRNA poly(A) tails having a predetermined length, separating the first and second samples by a chromatography method, which result in one or more chromatograms, and determining a sequence length of the mRNA poly(A) tails by comparing the chromatograms of the first and second samples. Chromatography methods for analyzing the poly(A) tail may include ultraviolet size-exclusion chromatography (SEC UV), ultraviolet ion-pair reversed-phase liquid chromatography (IP RP LC UV), ultra high performance liquid chromatography (UPHLC), and combinations thereof.

公开(公告)号：US11842891B2

公开(公告)日：2023-12-12

申请号：US17225514

申请日：2021-04-08

Applicant: Waters Technologies Corporation

Inventor： Joseph A. Jarrell ,  Martin Gilar

IPC: H01J49/02 ,  G01N27/623 ,  H01J49/00 ,  H01J49/16 ,  H01J49/40 ,  G01N30/62

CPC classification number: H01J49/025 ,  G01N27/623 ,  G01N30/62 ,  H01J49/0031 ,  H01J49/164 ,  H01J49/40

Abstract: An ion detector comprises a surface configured to receive one or more ions and a detector configured to detect one or more ions by detecting electromagnetic radiation scattered by one or more ions at the surface.